Developing near real-time DEP hardware Nahrizul Adib Kadri [email_address]
What’s DEP? <ul><li>Electrophoresis </li></ul><ul><ul><li>Charged particles moving in uniform field </li></ul></ul>
What’s DEP? <ul><li>Dielectrophoresis </li></ul><ul><ul><li>Dielectric particles moving in non-uniform field </li></ul></u...
What’s DEP? <ul><li>Theory bit </li></ul><ul><ul><li>(the only time an equation appears in this presentation… promise!) </...
What’s DEP used for? <ul><li>Cell movement can be manipulated by frequency changes, so DEP is good for cell  collection  a...
What’s DEP used for? <ul><li>Compare cell viability </li></ul>G.H. Markx, C.L. Davey / Enzyme and Microbial Technology 25 ...
What’s DEP used for? <ul><li>Compare effects of chemicals on cells </li></ul>F.H. Labeed et al. / Biochimica et Biophysica...
What’s DEP used for? <ul><li>Predict cell properties </li></ul>
What’s DEP used for? <ul><li>Everything’s fine and dandy  BUT  DEP is not available commercially, nor used widely </li></u...
What’s DEP used for? <ul><li>The answer(?):  Multiple experiments at the same time ! </li></ul><ul><ul><li>Different frequ...
http://www.youtube.com/watch?v=lLZbr3vP-Zs
DEP: The setup <ul><li>What’s needed: </li></ul>Imaging/recording device Microscope Electrode Cells Signal generator
DEP: The (new) setup <ul><li>What’s (really) needed: </li></ul>Imaging/recording device Microscope Electrode Cells Multipl...
Real-time DEP
Real-time DEP
Real-time DEP
Real-time DEP <ul><li>That’s it! (for now, hopefully) </li></ul><ul><li>Any questions:  </li></ul><ul><ul><li>[email_addre...
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100428 ug2

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A short introduction on DEP and the work that I'm doing at Biomedical Engineering Centre, University of Surrey

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100428 ug2

  1. 1. Developing near real-time DEP hardware Nahrizul Adib Kadri [email_address]
  2. 2. What’s DEP? <ul><li>Electrophoresis </li></ul><ul><ul><li>Charged particles moving in uniform field </li></ul></ul>
  3. 3. What’s DEP? <ul><li>Dielectrophoresis </li></ul><ul><ul><li>Dielectric particles moving in non-uniform field </li></ul></ul>Positive DEP Negative DEP
  4. 4. What’s DEP? <ul><li>Theory bit </li></ul><ul><ul><li>(the only time an equation appears in this presentation… promise!) </li></ul></ul>
  5. 5. What’s DEP used for? <ul><li>Cell movement can be manipulated by frequency changes, so DEP is good for cell collection and sorting </li></ul>Morgan et al. Biophysical Journal 77 (1999) 516-525
  6. 6. What’s DEP used for? <ul><li>Compare cell viability </li></ul>G.H. Markx, C.L. Davey / Enzyme and Microbial Technology 25 (1999) 161–171
  7. 7. What’s DEP used for? <ul><li>Compare effects of chemicals on cells </li></ul>F.H. Labeed et al. / Biochimica et Biophysica Acta 1760 (2006) 922–929
  8. 8. What’s DEP used for? <ul><li>Predict cell properties </li></ul>
  9. 9. What’s DEP used for? <ul><li>Everything’s fine and dandy BUT DEP is not available commercially, nor used widely </li></ul><ul><ul><li>Despite being described back in 1950s </li></ul></ul><ul><li>… but why? </li></ul><ul><ul><li>Small assays only </li></ul></ul><ul><ul><ul><li>Restricted to research use </li></ul></ul></ul><ul><ul><li>Time consuming </li></ul></ul><ul><ul><ul><li>One spectrum takes 40-60 minutes </li></ul></ul></ul>
  10. 10. What’s DEP used for? <ul><li>The answer(?): Multiple experiments at the same time ! </li></ul><ul><ul><li>Different frequencies, or </li></ul></ul><ul><ul><li>Same frequencies </li></ul></ul>10kHz 2MHz 300um
  11. 11. http://www.youtube.com/watch?v=lLZbr3vP-Zs
  12. 12. DEP: The setup <ul><li>What’s needed: </li></ul>Imaging/recording device Microscope Electrode Cells Signal generator
  13. 13. DEP: The (new) setup <ul><li>What’s (really) needed: </li></ul>Imaging/recording device Microscope Electrode Cells Multiple output signal generator
  14. 14. Real-time DEP
  15. 15. Real-time DEP
  16. 16. Real-time DEP
  17. 17. Real-time DEP <ul><li>That’s it! (for now, hopefully) </li></ul><ul><li>Any questions: </li></ul><ul><ul><li>[email_address] </li></ul></ul><ul><ul><li>facebook.com/nahrizuladib </li></ul></ul>
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