the diiference between serum and plasma

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the diiference between serum and plasma

  1. 1. By; - Nahom Ephrem and Selamawit Demeke
  2. 2. Introduction  Blood, fluid pumped by the heart that circulates throughout the body via the arteries, veins, and capillaries  An adult man of average size normally has about 5.6 liters of blood  Although blood appears to be red liquid it is actually composed of yellowish liquid called plasma and billions of cells.
  3. 3. Cont’d…  Blood = Cells + plasma  Plasma is a clear, straw-colored liquid portion of the blood in which the other cells are suspended.  Serum is the undiluted, extracellular portion of blood after adequate coagulation is complete.  In laboratory they can be separated and measured in order to help with the diagnosis and monitoring of diseases.
  4. 4. What is plasma?  Plasma is a clear, straw-colored liquid portion of the blood in which the other cells are suspended. Or  Plasma = water + proteins + dissolved substances  It is 90-92 percent water  Plasma is a transporting medium for cells and a variety of substances vital to the human/animals body.  Importantly, plasma contains proteins for blood clotting and defending the body against infection.
  5. 5. Preparation / Separation of plasma 1. The blood is mixed with an appropriate amount of anticoagulant like: - --Heparin, --Oxalate or --Ethylene Di-amine Tetra Acetic acid (EDTA)  This preparation should be mixed immediately and thoroughly to avoid clotting. 2. The solution is then centrifuged for 5-10 minutes at 2000 -2500 rpm. 3. The supernatant fluid is then separated and then labeled properly.
  6. 6. Cont’d… Plasma proteins Albumins = 60% Globulins = 35% Fibrinogen = 4% The other 1% of blood protein content = regulatory proteins, lipoproteins, iron-binding proteins With anticoagulant Component Percent Water ~92 Proteins 6–8 Salts 0.8 Lipids 0.6 Glucose (blood sugar) 0.1
  7. 7.  Serum  The clear liquid that can be separated from clotted blood  Blood serum is about 90% water with dissolved proteins, minerals, hormones and carbon dioxide and is an important source of electrolytes  In blood, the serum is the component that is neither a blood cell (serum does not contain white or red blood cells) nor a clotting factor; it is the blood plasma with the fibrinogens removed i.e.  Serum = Plasma - Clotting factors
  8. 8.  Preparation / Separation of Serum  The blood is allowed to clot at room temperature for 15 to 30 minutes.  When the blood has clotted completely, it is rimmed or ringed with an applicator stick,  Then centrifuged for 5-10 minutes at 2,500 revolutions per minute (rpm).  Finally the supernatant fluid is then separated making use of a Pasteur pipette, and labeled accordingly.
  9. 9. No anticoagulant
  10. 10. Summary  Plasma  Serum  Fluid obtained when anti- coagulated blood has been centrifuged  Anti-coagulants are needed for separation  Fibrinogen is present in plasma  Does not need "standing"; it could be centrifuged as soon as it has been mixed thoroughly.  plasma is delivered to the patients who lack blood cells  Fluid obtained when coagulated blood has been centrifuged  Anti-coagulants are not needed  Fibrinogen is absent  Serum takes a longer time to prepare  Serum is the most preferred part of blood used in checking blood groups and diagnosis of diseases

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