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My#Role#on#the#Project#
/  Tes2ng#an2bodies#
/  Determining#sensi2vity#of#IRIS#for#pseudotyped#VSV#detec2on#
/  Determinin...
Hi, my name is Collaborator X and
I'm responsible for optimizing the
protocol that we use to coat the
chips with antibodie...
Read the full story
behind this
slide makeover
Chip%Prepara*on%Protocol%
Chip%Spo(ng%&%Washing:%
%
0%An*body%solu*ons%are%spo7ed%at%305%mg/ml%in%PBS%with%50%mM%Trehalose...
Spotting and Washing Pre-incubation scan
Incubation with virus Post-incubation scan
500
520
540
560
580
600
LED
LED
Future&Work&
*  Dilu.on&experiment&for&Marburg*pseudotyped&VSV&
*  10^4&PFU/ml&detec.on&in&30&minutes&
&
*  Tes.ng&of&New&...
Obtain dilution curves for!
Marburg- and Lassa-pseudotype VSV
Obtain dilution curves for!
Marburg- and Lassa-pseudotype VSV
Try to detect viruses at lower !
concentrations (104 PFU/ml)...
Obtain dilution curves for!
Marburg- and Lassa-pseudotype VSV
Try to detect viruses at lower !
concentrations (104 PFU/ml)...
Click on the envelope
to get an email with
the next slide makeover
(probably on sunday)
3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2
3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2
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3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

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3 Ways to Obliterate Bullet Points From Your Slides

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3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

  1. 1. awesome presentations newsletter ! slide makeover 2
  2. 2. My#Role#on#the#Project# /  Tes2ng#an2bodies# /  Determining#sensi2vity#of#IRIS#for#pseudotyped#VSV#detec2on# /  Determining#repeatability# /  Valida2ng#new#IRIS#instruments#(IRIS#on#med#campus)# /  Standardizing#the#chip#prepara2on/incuba2on#protocol## /  To#improve#sensi2vity#and#repeatability#with#surface#op2miza2on#
  3. 3. Hi, my name is Collaborator X and I'm responsible for optimizing the protocol that we use to coat the chips with antibodies. My job is to make sure that each chip has as few foreign particles as possible, and that each antibody has a high affinity for the virus we are trying to detect. After incubating the chips, I scan them and analyze the data. Then, I try to come up with ways to lower the variability and improve the sensitivity of our results. “
  4. 4. Read the full story behind this slide makeover
  5. 5. Chip%Prepara*on%Protocol% Chip%Spo(ng%&%Washing:% % 0%An*body%solu*ons%are%spo7ed%at%305%mg/ml%in%PBS%with%50%mM%Trehalose.%%% %% 0%ADer%overnight%incuba*on%in%the%spo7er%(at%60%%humidity),%chips%are%washed%in% PBST%for%30%minutes,%rinsed%in%1X%PBS%for%3%minutes%and%dI%for%3%minutes%and%dried% with%argon.% Incuba4on%of%Chips%with%Concentrated%Samples:% ! 0%Chips%are%scanned%on%IRIS%for%pre0incuba*on%par*cle%counts.% %% 0%Chips%are%incubated%with%1%ml%of%the%virus%sample%for%2%hours%on%shaker.%% 0%ADer%incuba*on%ends,%chips%are%washed%in%1X%PBS%for%3%*mes,%each%1%min%and%then% 0.1X%PBS%for%1%min,%dipped%in%dI%for%10%seconds%and%pulled%out%of%the%water%slowly%to%let% the%water%slide%down%the%chip.% %% 0%Chips%are%scanned%with%IRIS%for%analysis.%
  6. 6. Spotting and Washing Pre-incubation scan Incubation with virus Post-incubation scan 500 520 540 560 580 600 LED LED
  7. 7. Future&Work& *  Dilu.on&experiment&for&Marburg*pseudotyped&VSV& *  10^4&PFU/ml&detec.on&in&30&minutes& & *  Tes.ng&of&New&Lassa&An.bodies&(7&of&them)&&& *  Dilu.on&curve&for&Lassa*pseudotyped&VSV&for&LOD&determina.on&
  8. 8. Obtain dilution curves for! Marburg- and Lassa-pseudotype VSV
  9. 9. Obtain dilution curves for! Marburg- and Lassa-pseudotype VSV Try to detect viruses at lower ! concentrations (104 PFU/ml) after ! shorter incubation periods (30 minutes)
  10. 10. Obtain dilution curves for! Marburg- and Lassa-pseudotype VSV Try to detect viruses at lower ! concentrations (104 PFU/ml) after ! shorter incubation periods (30 minutes) Test 7 new Lassa antibodies
  11. 11. Click on the envelope to get an email with the next slide makeover (probably on sunday)
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