Your SlideShare is downloading. ×
How fat stem cells could or not form muscle cells (Eng)
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×
Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply

How fat stem cells could or not form muscle cells (Eng)

1,480

Published on

Published in: Health & Medicine, Technology
1 Comment
0 Likes
Statistics
Notes
  • Be the first to like this

No Downloads
Views
Total Views
1,480
On Slideshare
0
From Embeds
0
Number of Embeds
1
Actions
Shares
0
Downloads
11
Comments
1
Likes
0
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
No notes for slide

Transcript

  • 1. hMADS cells myogenic potentiality an in vitro investigation Ari Massoudi UMR 6543 CNRS « Stem cells and differentiation » Centre de Biochimie Université de Nice-Sophia Antipolis Faculte des Sciences
  • 2. in vitro culture and expansion of adherent cells adipocytes Stroma-vascular cells Adipose tissues from human neonate and infant donors human Multipotent Adipose-Derived Stem cells (hMADS cells) after several months Rodriguez et al. J Exp Med . 2005.
    • self-renewal abitily
    • high telomerase activity
    • normal karyotype
    • non-tumorigenic
    • multipotency
    hMADS cells present fundamental stem cells properties :
  • 3. Mesenchymal Multipotence ALP Ostéonectine Ostéocalcine Ostéopontine Col I  1 … CBFA-1 Osterix SMADs VD 3 R FosB Adipocytes CD36 GAPDH LPL aFABP Leptine Adiponectine Adipsine … Pref-1 C/EBP  C/EBP  PPAR  PPAR  C/EBP  Induction adipogénique Induction ostéogénique progéniteur précurseur Ostéocytes Alizarin red ORO hMADS cells
  • 4. In vivo hMADS cell myofiber contribution hMADS cells intra-muscular injection previous work of the lab : Human dystrophin expression hMADS nuclei detected in muscles Rodriguez et al. J Exp Med . 2005. several months later mdx mouse Aims : to understand how hMADS cells contibute to skeletal muscle
  • 5. proliferation proliferating myoblasts cellular fusion differentiated myotubes Myogenesis quiescent myoblasts activated myoblasts G0/G1transition at confluency G1/G0 transition by contact inhibition commitment MyoD myf5 Pax7 MyoD myf5 myogenin Myogenin Herculin
  • 6. nuclear-LacZ hMADS cells mouse C2C12 myoblasts + human nuclei  -gal + human- mouse hybrid myotubes hMADS cells contribute to myotube formation Similar results with : primary wt or mdx mouse myoblasts primary wt human myoblasts human myoblasts from Duchenne Muscular Dystrophy
  • 7. hMADS cells in co-culture expressed human muscle genes D 0 D 1 D 2 D 4 hMADS cells / C2C12 co-cultures h = human specific primers m = mouse specific primers h sarcospan h dystrophin h muscle creatine kinase h enolase 3 h desmin h actin m hprt muscle markers human muscle cells mouse C2C12 RT-PCR
  • 8. hMADS/C2C12 hybrid myotubes expressed human  -sarcoglycan protein hMADS nuclei stained with anti- human nuclei Ab anti human  sarcoglycan ( confocal analysis )
  • 9. Human Dystrophin-defective myotubes (from DMD myoblasts) hMADS / DMD co-culture dystrophin staining hMADS cells complemente dystrophin deficiency in human DMD myotubes
  • 10. 3) How have hMADS cells contributed to myotube formation ? 1) hMADS cells are able to fuse with myoblasts 2) After fusion, hMADS cells express muscle genes
  • 11. Hypothesis : Do myoblasts induce myogenic transdetermination of hMADS cells ? Strategy : Assessing " key " Muscle Determination Factors (MDF) expression by hMADS cells in co-cultures How have hMADS cells contributed to myotube formation ? Pax7 ? MyoD ? Myogenin ? hMADS cells Myoblasts inducing factors ? transdetermination « myo »-hMADS cells
  • 12. Myogenin + myoblasts GFP-hMADS cells in co-culture do not express Muscle Determination Factors hMADS cell myotube contribution was not due to transdetermination Similarly : GFP-hMADS cells Myoblasts : Pax7+ GFP-hMADS cells : Pax7- Myoblasts : MyoD+ GFP-hMADS cells : MyoD-
  • 13. Mouse encoded-myogenin in hMADS nuclei of hybid myotubes GFP hMADS nucleus Hoescht smooth ponctuate murine nucleus merge myoG hum myogenin hum actin mouse hprt hMADS/C2C12 co-culture myotubes RT-PCR human muscle cells mouse C2C12
  • 14. myoblasts myotubes human myoblasts GFP-hMADS + mouse C2C12 human nestin staining differentiation No cross-reaction with mouse nestin Human nestin was expressed " only " in hMADS/C2C12 myotubes
  • 15. Conclusion Post-fusion conversion by the myotube differentiation factors By a classical Determination / Differentiation process 2 nd hypothesis 1 st hypothesis Myogenic conversion of hMADS nuclei :  sarcoglycan + dystrophin + nestin + sarcospan + desmin + enolase3 + muscle creatin kinase + Myogenin-expressing myoblasts hMADS cells cellular fusion myotube MDFs ( myogenin, herculin, MEFs ) Model of hMADS myogenic conversion
  • 16. Perspectives Myogenic conversion of hMADS nuclei :  sarcoglycan + dystrophin + nestin + sarcospan + desmin + enolase3 + muscle creatin kinase + myotube MRFs ( myogenin, herculin, MEFs ) MDF involement in hMADS nuclei reprogramation Factors involved in hMADS / myoblasts fusion Global hMADS gene modification Myogenin-expressing myoblasts hMADS cells cellular fusion
  • 17.  
  • 18.
    • 46 conditions de culture testées en présence ou en l’absence de sérum
    • milieux conditionnés provenant de myoblastes
    • membrane natives provenant de myoblastes
    • différents type de matrice extracellulaire
    Aucune des conditions testées n’a permit d’induire un programme myogénique Conditions de culture testées pour promouvoir la myogenèse des cellules hMADS
  • 19. Expression de Nestine humaine Co-culture cellules hMADS / myoblastes C2C12 souris Myotube hybride Myotubes humain * * * *
  • 20. Cellules souches (CS) Progéniteurs (Pro) Auto-renouvellement Condition obligatoire, mais… L’auto-renouvellement peut aussi concerner les Pro Multipotence Certaines CS sont « unipotentes » (spermatogonies) Certains Pro sont « multipotents » Clonogénicité Certaines cellules souches ne poussent pas ou mal en dilution clonal Clonogénicté est aussi vrai pour les Pro Phénotype « side population » Controversé ! Ne concernent que quelques rares CS - Activité télomérase Ne concernent que quelques rares CS - Activité phagocytaire ++++ + Non-immunogénicité Vrai pour quelques rares cellules souches (MSC) +/- Marqueurs spécifiques Ne concernent que quelques rares CS (cellules ES, HSC, cellules satellites…)

×