CYTOLOGY OF CSF

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CYTOLOGY OF CSF

  1. 1. PRESENTATION ON“CYTOLOGY OF CSF”BYMAIMOON ZAFAR
  2. 2. The cerebrospinal Fluid [CSF] isclearcolorlesstransparentThe Tissue fluid present in the cerebral ventriclesspinal canal
  3. 3. ProteinsGlucoseCholesterolNa+Ca+UreaCreatinineLactic acid
  4. 4. Lumbar puncture or spinal tap
  5. 5. Check Physical Appearance (gross)Slide Making (cytocentrifuge technique)Staining of slidesMicroscopic evaluation of slides
  6. 6. PHYSICAL APPEREANCENormal• Crystal clear, colorlessAbnormal• hazy, cloudy, turbid, milky, bloody, Xanthrochromic• Unclear specimens may contain increased lipids, proteins,cells or bacteria• Clots indicate traumatic tap• Milky – increased lipids• Oily – contaminated with x-ray• Xanthrochromic – Yellowing discoloration of supernatant(may be pinkish, or orange• Brown or Dark CSF –methemoglobin from a hematoma.• Green CSF --hyperbilirubinaria
  7. 7.  PHYSICAL APPEREANCE
  8. 8. CYTOCENTRIFUGE TECHNIQUE Use of centrifugal force to direct specimen onto cytoslides 2 slides are made cause sample is in small quantity
  9. 9.  1-2 drops sample + 1-2 drops of alcohol (95%) Spin at 600 rpm for 5 mins Remove and fix in alcohol
  10. 10. STAINING TECHNIQUEOF CSF SLIDESSTAININGPapanicolaou stain done Harris heamtoxylin(nuclear stain) OG-6 (cytoplasm satin ) EA 50 (immature cells) Heamtoxlyin Water Acid water Water Lithium carbonate 70% ehtyl alcohol 80%ethyl alcohol 95%ehtyl alcohol OG-6 EA 50 95% ethyl alcohol Xylene phenol Xylene Cover slip/ DPX
  11. 11. Normal CSF is essentially acellularEntire smear should be evaluated for• abnormal cells• inclusions within cells• Clusters• Presence of intracellular organism
  12. 12. Types of cells Lymphocytes & monocytes / macrophages Malignant cells Ependymal cells (epithelial-like cells) Leukemia cells Neutrophils RBC WBC
  13. 13. Meningeal infections• viral,bacterial,fungal• Clear to cloudySubarachnoid hemorrhage• ruptured cerebral aneurysm, or may resultfrom head injury• Bloody CSFCNS malignancy• Brain tumors
  14. 14. Lymphocytes & monocytes /macrophagesMono / macro, segs and lymph
  15. 15. Ependymal cellsNeutrophils
  16. 16. malignant cells Eosinophils
  17. 17. leukemic cells
  18. 18. • To confirm diagnosis of a disease• Evaluate for intracranial hemorrhage• Diagnose malignancies, leukemia• Investigate central nervous system disorders
  19. 19. THANK YOU FOR YOURPATIENCE ANY QUESTIONS?

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