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CYTOLOGY OF CSF
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CYTOLOGY OF CSF

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  • 1. PRESENTATION ON“CYTOLOGY OF CSF”BYMAIMOON ZAFAR
  • 2. The cerebrospinal Fluid [CSF] isclearcolorlesstransparentThe Tissue fluid present in the cerebral ventriclesspinal canal
  • 3. ProteinsGlucoseCholesterolNa+Ca+UreaCreatinineLactic acid
  • 4. Lumbar puncture or spinal tap
  • 5. Check Physical Appearance (gross)Slide Making (cytocentrifuge technique)Staining of slidesMicroscopic evaluation of slides
  • 6. PHYSICAL APPEREANCENormal• Crystal clear, colorlessAbnormal• hazy, cloudy, turbid, milky, bloody, Xanthrochromic• Unclear specimens may contain increased lipids, proteins,cells or bacteria• Clots indicate traumatic tap• Milky – increased lipids• Oily – contaminated with x-ray• Xanthrochromic – Yellowing discoloration of supernatant(may be pinkish, or orange• Brown or Dark CSF –methemoglobin from a hematoma.• Green CSF --hyperbilirubinaria
  • 7.  PHYSICAL APPEREANCE
  • 8. CYTOCENTRIFUGE TECHNIQUE Use of centrifugal force to direct specimen onto cytoslides 2 slides are made cause sample is in small quantity
  • 9.  1-2 drops sample + 1-2 drops of alcohol (95%) Spin at 600 rpm for 5 mins Remove and fix in alcohol
  • 10. STAINING TECHNIQUEOF CSF SLIDESSTAININGPapanicolaou stain done Harris heamtoxylin(nuclear stain) OG-6 (cytoplasm satin ) EA 50 (immature cells) Heamtoxlyin Water Acid water Water Lithium carbonate 70% ehtyl alcohol 80%ethyl alcohol 95%ehtyl alcohol OG-6 EA 50 95% ethyl alcohol Xylene phenol Xylene Cover slip/ DPX
  • 11. Normal CSF is essentially acellularEntire smear should be evaluated for• abnormal cells• inclusions within cells• Clusters• Presence of intracellular organism
  • 12. Types of cells Lymphocytes & monocytes / macrophages Malignant cells Ependymal cells (epithelial-like cells) Leukemia cells Neutrophils RBC WBC
  • 13. Meningeal infections• viral,bacterial,fungal• Clear to cloudySubarachnoid hemorrhage• ruptured cerebral aneurysm, or may resultfrom head injury• Bloody CSFCNS malignancy• Brain tumors
  • 14. Lymphocytes & monocytes /macrophagesMono / macro, segs and lymph
  • 15. Ependymal cellsNeutrophils
  • 16. malignant cells Eosinophils
  • 17. leukemic cells
  • 18. • To confirm diagnosis of a disease• Evaluate for intracranial hemorrhage• Diagnose malignancies, leukemia• Investigate central nervous system disorders
  • 19. THANK YOU FOR YOURPATIENCE ANY QUESTIONS?