2 8 Mutation

1,531 views
1,396 views

Published on

Published in: Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
1,531
On SlideShare
0
From Embeds
0
Number of Embeds
1
Actions
Shares
0
Downloads
52
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

2 8 Mutation

  1. 1. <ul><li>1. Convert the following mRNA strand into DNA : </li></ul><ul><li> … CUGACGUACG…. </li></ul><ul><li>2. Take the following DNA strand through Central Dogma and produce the protein : </li></ul><ul><li> GACATACTCAACTGCAATT </li></ul>
  2. 2. <ul><li>Convert the following mRNA strand into DNA: </li></ul><ul><li>… CUGACGUACG… </li></ul><ul><li>GACTGCATGC </li></ul><ul><li>Take the following DNA strand through Central Dogma and produce the amino acid chain: </li></ul><ul><li>GACATACTCAACTGCAATT </li></ul><ul><li>CUGU AUG-AGU-UGA-CGUUAA </li></ul><ul><li>Met- Ser-Stop </li></ul>
  3. 3. <ul><li>DNA and DNA replication (Mon) </li></ul><ul><li>Transcription (Wed) </li></ul><ul><li>CSI Las Vegas (Thu) </li></ul><ul><li>Translation and Transcription Practice (Fri) </li></ul>
  4. 4. <ul><li>B1 </li></ul><ul><li>B2 </li></ul><ul><li>B3 </li></ul><ul><li>B5 </li></ul>
  5. 6. <ul><li>A mutation is a change in DNA sequence (order of nucleotides). </li></ul>
  6. 7. <ul><li>Mutations are important because they increase genetic variation . </li></ul>
  7. 8. <ul><li>Mutations can be good as well as bad. </li></ul><ul><li>Ex: A Protein to run faster </li></ul><ul><li>Ex: A protein that causes Sickle Cell Anemia </li></ul>
  8. 9. <ul><li>A mutagen is something that causes a mutation. </li></ul><ul><li>Ex: radiation, chemicals, high temp. </li></ul>
  9. 10. <ul><li>There are 2 main types of gene mutations. </li></ul><ul><ul><li>Point mutations </li></ul></ul><ul><ul><li>Frameshift mutations </li></ul></ul>
  10. 11. <ul><li>Point mutation-a change in one base pair in a DNA sequence. </li></ul><ul><li>Example: A U G=Met </li></ul><ul><li> A A G=Lys </li></ul>
  11. 12. <ul><li>A point mutation may cause an amino acid to change, which will change the structure of the protein being made. </li></ul><ul><li>We call this a MISSENSE mutation </li></ul>
  12. 13. Normal Point mutation mRNA Protein Stop Stop mRNA Protein Replace G with A
  13. 14. <ul><li>If the point mutation results in the same amino acid produced, we call this a SILENT mutation </li></ul><ul><li>AG U  AG C </li></ul><ul><li>SER  SER </li></ul>
  14. 15. <ul><li>Frameshift mutation- adding or deleting nucleotides to a DNA sequence. </li></ul><ul><li>A frameshift mutation is much worse than a point mutation because it causes the entire sequence to be thrown off </li></ul>
  15. 16. <ul><li>A mutation the ends in an early stop codon is called a nonsense mutation. </li></ul>
  16. 17. <ul><li>DNA: THE CAT ATE THE HAT </li></ul><ul><li> </li></ul><ul><li> </li></ul><ul><li>We get: HEC ATA TET HEH AT </li></ul>Delete this T
  17. 18. mRNA Protein Frameshift mutation
  18. 20. <ul><li>Is this a point mutation or a frameshift mutation? </li></ul><ul><li>It’s a point mutation because only one nucleotide changed! </li></ul>
  19. 21. <ul><li>THE DOG BIT THE CAT </li></ul><ul><li>THE DOG BIT THE CAR </li></ul><ul><li>Point or frameshift? </li></ul><ul><li>Point! </li></ul>
  20. 22. <ul><li>THE DOG BIT THE CAT </li></ul><ul><li>THE DOB ITT HEC AT </li></ul><ul><li>Point or frameshift? </li></ul><ul><li>-frameshift </li></ul>

×