Biosynthesis of amino acids

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  • 1. Biosynthesis of Amino Acids M.Prasad Naidu MSc Medical Biochemistry, Ph.D,.
  • 2. Overview
  • 3. Overview (cont)
  • 4. Biosynthetic Families Metabolic Precursors Amino Acids -ketoglutarate Glutamate Glutamine Proline Arginine 3-Phophoglycerate Serine Glycine Cysteine
  • 5. Biosynthetic Families (cont) Metabolic Precursors Amino Acids Oxaloacetate Aspartate, Asparagine Methionine, Threonine, Lysine Pyruvate Alanine, Valine, Leucine Isoleucine
  • 6. Biosynthetic Families (cont) Metabolic Precursors Amino Acids Phosphoenolpyruvate Tryptophan and erythrose 4-phosphate Phenlyalanine Tyrosine Ribose 5-phophate Histidine
  • 7. Ketoglutarate Family Ketoglutarate Glutamate Glutamine Proline Arginine
  • 8. -Ketoglutamate to glutamate/glutamine Glutamate + NH4 + + ATP Glutamine synthetase Glutamine + ADP + Pi + H+ -Ketoglutamate + glutamine + NADPH + H+ Glutamate synthase 2 glutamate + NADP+
  • 9. -Ketoglutamate to glutamate/glutamine Net Reaction: -Ketoglutarate + NH4 + + NADPH + ATP L-glutamate + NADP+ + ADP + Pi
  • 10. Ketoglutarate Family Ketoglutarate Glutamate Glutamine Proline Arginine
  • 11. Synthesis of Proline
  • 12. Synthesis of Proline
  • 13. Synthesis of Arginine
  • 14. Synthesis of Arginine
  • 15. 3-Phosphoglycerate Family 3-Phosphoglycerate Serine Glycine Cysteine
  • 16. Synthesis of Serine and Glycine
  • 17. Synthesis of Cysteine
  • 18. Oxoloacetate Family Oxaloacetate Aspartate Asparagine Methionine Lysine Threonine
  • 19. Synthesis of Aspartate and Asparagine Oxaloacetate AspartateTransamination from glutamate Amidation with Glutamine donating NH4 + Asparagine
  • 20. Synthesis of Lysine, Methionine, and Threonine • See handout
  • 21. Pyruvate Family Pyruvate Alanine Valine Leucine Isoleucine
  • 22. Synthesis of Alanine Pyruvate AlanineTransamination from glutamate
  • 23. Synthesis of Isoleucine, Valine,and Leucine • See handout
  • 24. Phosphoenolpyruvate and erythrose 4-phosphate Family Phosphoenolpyruvate + erythrose 4-phosphate Chorismate Tryptophan Tyrosine Phenylalanine
  • 25. Synthesis of Chorismate
  • 26. Synthesis of Tryptophan
  • 27. Synthesis of Tyrosine and Phenylalanine
  • 28. Synthesis of Tyrosine and Phenylalanine (cont)
  • 29. Biosynthesis of Histidine • See handout
  • 30. Possible Test Questions 1) Provide the 6 metabolic precursors and the amino acids which they lead to. 2) Duplicate the Amino Acid Synthesis Overview Flow Chart. Expand the sections need to show the synthesis of Glutamate, Glutamine, Proline, Arginine, Serine, Cysteine, Glycine, and starting from Chorismate Tryptophan, Tyrosine and Phenylalanine. (40 points!!)