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산모 흡연 연구과제
 

산모 흡연 연구과제

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    산모 흡연 연구과제 산모 흡연 연구과제 Presentation Transcript

    • 임신 초기 임신부의흡연 노출 평가진단검사의학과곽호석
    • Smoking patterns during pregnancy평균 4주평균 7주
    • Quantitation of Metabolites of NNK afterCessation of Smokeless Tobacco UseTime post-cessation (days)SmokingNNAL t1/2: 10-18 days > cotinine t1/2 : 1days0.0%20.0%40.0%60.0%80.0%100.0%120.0%0 2 4 6 8 10 12 14 16 18 20Cocncentration(%)DayCotinineNNAL
    • The metabolic activation andinactivation of NNK* 11β-HSD: 11β-hydroxysteroid dehydrogenaseSteroid hormonesDrug: diureticsDrug: block 11β-HSDDrugDrug: flavonoidFlavanoneSimilar structure: hormones
    • Total NNAL after exposure to sidestream smoke (4hr)
    • Urine Concentrations of a Tobacco-Specific NitrosamineCarcinogen in the U.S. Population from SecondhandSmoke Exposure
    • NNAL concentration according to cigarette brand미국 벡터(Vector)사는 지난 2001년 발암성 물질을 줄인 담배‘OMNI’를 선보인 데 이어, 2003년에는 저니코틴 담배 ‘QUEST’를출시했다. 이 담배는 기존 담배와 비교해 니코틴 함량을 97% 낮춘GM(유전자조작)담배로 제조됐다. 그러나 향과 맛 등의 주요 특징들은 기존 담배와 거의 동일한 것으로 전해졌다.A Vector Tobacco website claimed Omni produceda 53% reduction in exposure to NNK.
    • NNAL 분석
    • Comparison of analytical methodAuthor Urine vol. LOQ(pg/mL)Analyticalconcentration of LOQSample preparationHou Hongwei etal. (2012)5 mL 0.99 50 pg/mLEnzymereaction(overnight),MIPShowket H.Bhat et al.(2011) 0.25 mL 20 50 pg/mLEnzyme reaction(20h),derivatization(12h),SPEDominiqueKavvadias et al.(2009)6 mL 5 300 pg/mLEnzyme reaction(14h),MIP, SPEYang Xia et al.(2005) 5 mL 1.7 425 pg/mLEnzyme reaction(24h),MIP
    •  Sample preparation• Enzyme reaction: beta-glucuronidase (overnight)• NNAL extraction: MIP(molecular imprinting polymer) column (2hr)• Measurement of NNAL: LC-MS/MS (6min/sample)
    • Chromatograms of the ion transition for NNAL and itsdeuterated internal standard in urine sample of a smoker5 ng/mL 500 ng/mLNNAL-d3 NNAL-d3NNAL NNALUrine: 1mL (20cigarette/day, male)Non-enzyme digestion, MIP column
    • Microwave-Assisted Enzyme-Catalyzed Reactions
    • Metabolism of TSANNNA has been described as a veryreactive aldehyde which has neverbeen quantified in tobacco or tobaccosmoke. There has been considerableinterest in recent yearsin the chemical composition ofsmokeless tobacco products basedaround health concerns associatedwith their use
    • 경청해 주셔서 감사합니다.