Laboratory Centrifuge

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The centrifuge used to separate solids
suspended in a liquid by sedimentation utilizing rotational movements in controlled periods of time.

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Laboratory Centrifuge

  1. 1. Centrifuge  The centrifuge used to separate solids suspended in a liquid by sedimentation.  The rotational movements allow forces much greater than gravity to be generated in controlled periods of time.  In the laboratory, centrifuge can used to separate blood components: red cells, white cells, platelets to carry out further analysis tests and treatments.  There is a wide range of centrifuges capable of serving specific industry and research.
  2. 2. Theory of operation  When a body of mass [m] turns around a central point [O], it is subjected to a centripetal force [F] directed towards the rotation axis with a magnitude F = mω2r.  where [m] is the mass of the body, [r] is the radius and ω is the angular speed.  Denser particles will settle at the bottom of the tube in shorter periods of time, while lighter ones require longer periods of time.
  3. 3. Centrifuge
  4. 4. Components of the centrifuge  On and off control, operation time control (timer),       rotation speed control, temperature control (in refrigerated centrifuges), vibration control (safety mechanism) and brake system Refrigeration system (in refrigerated centrifuges). Base Lid/cover Casing Electric motor Rotor. There are different types of rotors. The most common are the fixed angle, the swinging buckets, the vertical tube and the almost vertical tube types.
  5. 5. Preventive Maintenance Monthly Annually   Verify that electronic cards are clean and     Verify that the centrifuge external components are free of dust and stains. Clean the rotor compartment using a mild detergent. Test that the rotors’ connecting and adjustment mechanisms are in good condition. Keep the points lubricated as the manufacturer recommends. Verify the locking /safety mechanism of the centrifuge’s cover. Check the lubrication state of elements such as for O-rings as the manufacturer recommends. Verify the state of gaskets and watertight joints.     well connected. Test operation controls needed for selection of the different parameters of the centrifuge: speed, time, temperature, alarms selectors and analogous or digital instruments. Verify compliance with electrical standards. Use an electric safety analyzer: earth resistance test, escaping current test. Verify the actual rotation speed against the selected one using a normal load. The testing is done with a tachometer or a photo tachometer. Confirm the functioning of the brake system.
  6. 6. There is a vibration. •The rotor is unbalanced.  Balance the rotor’s load. Fill all the opposite tubes with the same level of liquid of same density.  Distribute the weight of the opposite tubes symmetrically.  Load fixed angle or vertical tube rotors symmetrically  Select a rotation outside of the critical speed range. •The speed selected is near the rotor’s critical speed range  Select a rotation outside of the critical speed range. •The rotor is incorrectly mounted.  Verify the rotor’s assembly. Test that it is well adjusted •There is a lack of lubrication in the rotor’s supports.  Lubricate the pivoting axis according to the manufacturer’s recommendation.
  7. 7. The tubes are cracked or broken. The tubes can be broken or become fragile if they are used below the recommended temperature.  If the sample is frozen, warm to 2 °C The tubes become fragile with age and use.  Discard expired tubes, use new ones. before centrifuging. Evaluate how the tubes behave at low temperatures before centrifuging
  8. 8. Thank You.

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