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Laboratory Centrifuge
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Laboratory Centrifuge

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The centrifuge used to separate solids …

The centrifuge used to separate solids
suspended in a liquid by sedimentation utilizing rotational movements in controlled periods of time.

Published in: Education, Business, Technology
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  • 1. Centrifuge  The centrifuge used to separate solids suspended in a liquid by sedimentation.  The rotational movements allow forces much greater than gravity to be generated in controlled periods of time.  In the laboratory, centrifuge can used to separate blood components: red cells, white cells, platelets to carry out further analysis tests and treatments.  There is a wide range of centrifuges capable of serving specific industry and research.
  • 2. Theory of operation  When a body of mass [m] turns around a central point [O], it is subjected to a centripetal force [F] directed towards the rotation axis with a magnitude F = mω2r.  where [m] is the mass of the body, [r] is the radius and ω is the angular speed.  Denser particles will settle at the bottom of the tube in shorter periods of time, while lighter ones require longer periods of time.
  • 3. Centrifuge
  • 4. Components of the centrifuge  On and off control, operation time control (timer),       rotation speed control, temperature control (in refrigerated centrifuges), vibration control (safety mechanism) and brake system Refrigeration system (in refrigerated centrifuges). Base Lid/cover Casing Electric motor Rotor. There are different types of rotors. The most common are the fixed angle, the swinging buckets, the vertical tube and the almost vertical tube types.
  • 5. Preventive Maintenance Monthly Annually   Verify that electronic cards are clean and     Verify that the centrifuge external components are free of dust and stains. Clean the rotor compartment using a mild detergent. Test that the rotors’ connecting and adjustment mechanisms are in good condition. Keep the points lubricated as the manufacturer recommends. Verify the locking /safety mechanism of the centrifuge’s cover. Check the lubrication state of elements such as for O-rings as the manufacturer recommends. Verify the state of gaskets and watertight joints.     well connected. Test operation controls needed for selection of the different parameters of the centrifuge: speed, time, temperature, alarms selectors and analogous or digital instruments. Verify compliance with electrical standards. Use an electric safety analyzer: earth resistance test, escaping current test. Verify the actual rotation speed against the selected one using a normal load. The testing is done with a tachometer or a photo tachometer. Confirm the functioning of the brake system.
  • 6. There is a vibration. •The rotor is unbalanced.  Balance the rotor’s load. Fill all the opposite tubes with the same level of liquid of same density.  Distribute the weight of the opposite tubes symmetrically.  Load fixed angle or vertical tube rotors symmetrically  Select a rotation outside of the critical speed range. •The speed selected is near the rotor’s critical speed range  Select a rotation outside of the critical speed range. •The rotor is incorrectly mounted.  Verify the rotor’s assembly. Test that it is well adjusted •There is a lack of lubrication in the rotor’s supports.  Lubricate the pivoting axis according to the manufacturer’s recommendation.
  • 7. The tubes are cracked or broken. The tubes can be broken or become fragile if they are used below the recommended temperature.  If the sample is frozen, warm to 2 °C The tubes become fragile with age and use.  Discard expired tubes, use new ones. before centrifuging. Evaluate how the tubes behave at low temperatures before centrifuging
  • 8. Thank You.

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