Autoclave

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Autoclave is a critical instrument used in laboratory,

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Autoclave

  1. 1. Sterilization  Sterilization is a term referring to any process that removes or kills all forms of microbial organisms such as fungi, bacteria, viruses, spore forms, etc. present on a surface, contained in a fluid, or in a compound such as biological culture media.  Sterilization can be achieved by applying heat, chemicals, irradiation, high pressure, and filtration or combinations of them.
  2. 2. The autoclave is a equipment used to remove microorganisms (Virus, Bacteria, fungus etc.) and spores using high pressure and high temperature steam sterilization.
  3. 3. PURPOSE OF THE AUTOCLAVE 1. To prepare materials for bacteriological cell cultures(test tubes, pipettes, Petri dishes, etc.) without contamination. 2. Prepare elements used for taking samples. (needles, tubes, containers). 3. Sterilize contaminated material.
  4. 4. OPERATION PRINCIPLES  when the pressure higher than atmosphere a higher     temperature than 1000C needs to boil the water. This steam contain higher energy than usual. Autoclaves make pressurized saturated steam in a chamber passing electrical current through a heating element in controlled manner. This energized steam take place required sterilization. This method is known as steam sterilization.
  5. 5. MAIN COMPONENTS 1. Heating Elements 2. Temperature Controller 3. Pressure Sensor 4. Chamber 5. Door gasket 6. Solenoid valve 7. Water level Sensor
  6. 6. BLOCK DIAGRAM
  7. 7. STERILIZATION PROCESS
  8. 8. Heating Elements
  9. 9. Temperature Controller Thermostat Pt 100
  10. 10. Pressure Sensor
  11. 11. Chamber
  12. 12. Door gasket
  13. 13. Solenoid valve
  14. 14. Water level Sensor
  15. 15. Symbols need to remind
  16. 16. Program selection Program nº Mode Temperature / pressure Sterilization time Drying time 1 Solid 121ºC / 1bar 20 - 2 Solid 126ºC / 1.33 bar 30 - 3 Solid 134C / 2 bar 18 - 4 Solid 134ºC / 2 bar 10 - 5 Liquid 121ºC / 1bar 30 - 6 Solid + Dry 121ºC / 1bar 30 35 7 Solid + Dry 126ºC / 1.33 bar 20 35 8 Solid + Dry 126ºC / 1.33 bar 18 35 9 Solid + Dry 134ºC / 2 bar 10 35 0 - - - -
  17. 17. Start 1. Main switch 2. Open door indicator lamp. 3. Lack of water indicator lamp. 4. End cycle indicator lamp. 5. Liquid mode selector. 6. Solid mode selector. 7. Solid+Drying mode selector. 8. Temperature indicator display. 9. Temperature select pushbutton. 10. Parameter up pushbutton. 1 1. Parameter down pushbutton. 12. Drying time indicator. 13. Time indicator display. 14. Time pushbutton. 15. Start pushbutton. 16. Program indicator display. 17. Program pushbutton. 18. Stop pushbutton. 20. Alarm indicator lamp.
  18. 18. Top view 21. Water drainage valve 22. Steam drainage valve. 23. Internal tank filling
  19. 19. Rear view 24. Air filter. 25. Safety valve. 26. Drying control thermostat. 27. Safety thermostat. 28. Drainage valve. 29. Steam drainage valve 30. Water inlet. 31. Condensation outlet 32. Tank drainage.
  20. 20. Cleaning Procedure  NEVER use bleach, steel wool, a steel brush or anything abrasive to scrub or clean the Chamber. 1. remove instruments and trays from the autoclave. 2. spread the contents of a packet in chamber evenly. 3. Select and start program No. 1 (without dry). 4. At the end of the cycle drain the water from the reservoir. 5. Fill the water reservoir with distilled water. 6. Repeat a sterilization cycle without Chamber Brite ™ powder, to remove any excessive dirt in the pipes. 7. Select and start program No. 1 (without dry). When the cycle is finished it will automatically exhaust. 8. At the end of the cycle drain the water from the reservoir. 9. Now autoclave ready to use.
  21. 21. Preventive Maintenances  Quarterly 1. Clean all the filters.(Water, Air ) 2. Fill the water level of feed tank up to maximum limit 3. Check that the manometers function as expected. 4. Check the accuracy of temperature reading. 5. Activate the safety valves manually to verify that they are operating well. 6. Conduct a general sterilization process write down pressure, temperature, required times for completing each phase of the cycle, put it on registration system. 7. Verify that it is functioning within tolerances defined by manufacturer 8. Check any abnormal sound in solenoids. 9. Clean the chamber and remove limescale if any, advice to use only distilled water. 10. Check the earth leakage current, protective earth.
  22. 22. limescale  Limescale is the hard, off- white, chalky deposit found in kettles, hot-water boilers  Add little amount of any acid heat the chamber.  Deposited CaCO3 will be removed.  Then rinse off with plenty of water.
  23. 23. Quality of Process  Sterilization process indicators  They check instrument’s control parameters like temperature, time      and pressure. Chemical indicators Adhesive tapes that go inside a component or on packages. Biological indicators The spore indicator is placed in the sterilizing load. After the process, it is incubated, analyzed and it is determined if the cycle meets with the sterilization requirements.

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