Nephrogenic diabetes insipidus, which involves the inability to concentrate urine or adjust to subtle changes in extracellular fluid osmolarity, results from the unresponsiveness of renal tubular osmoreceptors to ADH.
Dipole refers to unequal sharing of electrons in a chemical compound. A polar molecule has unequal sharing, where one side is more charged, such as water, the oxygen is negatively charged, and the hydrogen positive.A molecule with electrical charge distributed aymmetrically about its structureMaterials with a high dielectric constants are good insulators
Stomach -> HCl (parietal cells, decreases pH) needed by pepsinogen to be converted into pepsinMouth – carbohydrate digestionProtein – start in the stomach, due to pepsin and intrinsic factors (reabsorption vit B12)Mucus barrierGastrin controls secretionGastric emptying – increased by gastroenterogens???
Pancreatitis – due to the activation of zymogens
Maximum volume that the gallbladder can hold is only 30 to 60 milliliters.
>By far the most potent stimulus for causing the gallbladderContractions is the hormone cholecystokinin>Sphincter of oddi guards the exit of the common bile duct into the duodenum.
> It is important in the digestion and absorption of lipids>form micelles - with the hydrophobic sides towards the fat and hydrophilic towards the outside
Pancreatic problem – no absorption of ADEKBile – cholesterol, bile pigments, salts (must have equal concentrations) Imbalance: increase cholesterol bile stones
Iron and most soluble proteins is absorbed in Duodenum Bile acids are absorbed in terminal ileum
>Quite turbid because of the presence of >Nacl. Sodium bicarbonate, other inorganic salts>neutralize the acid chyme from stomach>ex. saccharidases, maltase, sucrase, lactase split saccharides, maltose, sucrose and lactose respectively
Despite this, water makes up about 70% of the fecal weight.
*Since the large intestine can only absorb about 8 liters of water in a day.
Separate hard lumps, like nuts (hard to pass).2. Sausage-shaped but lumpy.3. Like a sausage but with cracks on its surface.4. Like a sausage or snake, smooth and soft.5. Soft blobs with clear cut edges (passed easily).6. Fluffy pieces with ragged edges, a mushy stool.7. Watery stool, entirely liquid.>>>>>Types 1 and 2 indicate constipation, with 3 and 4 being the "ideal stools" especially the latter, as they are the easiest to pass, and 5–7 being further tending towards diarrhea or urgency.
While the concentration of glucose in a fluid does not necessarily help to determine if a fluid is a transudate or an exudate, it can help determine what might be causing an exudate.
Sodium potassium chlorine Low levels of magnesium and calcium
More casein than whey in infant formula milkWhey softens stools
Tanner 3 – milk production; due to ductile and lobular differentiationCitrate increases when it is near delivery
Contraction of myoepithelial cells (a fxn of oxytocin)
Seminar report body fluids
Body FluidsSection A, Group 2ALFONSO, ALJAMA, ALUZANAMURAO, ARELLANO, ARROYO
Water Predominant chemical component of living organisms Universal Solvent Has a dipolar structure and exceptional capacity for forming hydrogen bonds.
pH Scale The acidity of aqueous solutions is generally reported using the logarithmic pH scale. Bicarbonate and other buffers normally maintain the pH of extracellular fluid between 7.35 and 7.45
Water Molecules form Dipoles A water molecule is an irregular, slightly skewed tetrahedron with oxygen at its center.
Water Molecules Form Dipoles Water is a dipole Water, a strong dipole, has a high dielectric constant. Its strong dipole and high dielectric constant enable water to dissolve large quantities of charged compounds such as salts.
Water Molecules Form HydrogenBonds Hydrogen bonding favors the self-association of water molecules into ordered arrays .
Water Molecules Form HydrogenBonds Hydrogen bonding profoundly influences the physical properties of water and accounts for its exceptionally high viscosity, surface tension, and boiling point. Hydrogen bonding enables water to dissolve many organic biomolecules that contain functional groups which can participate in hydrogen bonding.
Interaction with Water Influencesthe Structure of Biomolecules
Covalent & Noncovalent BondsStabilize Biologic Molecules: These forces (covalent and noncovalent bonds), which can be either attractive or repulsive, involve interactions both within the biomolecule and between it and the water that forms the principal component of the surrounding environment.
Electrostatic Interactions Interactions between charged groups shape biomolecular structure. Electrostatic interactions between oppositely charged groups within or between biomolecules are termed salt bridges.
Van der Waals Forces Van derWaals forces arise from attractions between transient dipoles generated by the rapid movement of electrons on all neutral atoms.
Water is an excellent nucleophile Metabolic reactions often involve the attack by lone pairs of electrons on electron-rich molecules termed nucleophiles on electron-poor atoms called electrophiles. Nucleophiles and electrophiles DO NOT necessarily possess a formal negative or positive charge.
Water is an excellent nucleophile Water, whose two lone pairs of sp3 electrons bear a partial negative charge, is an excellent nucleophile Nucleophilic attack by water generally results in the cleavage of the amide, glycoside, or ester bonds that hold biopolymers together. This process is termed hydrolysis.
Water is an excellent nucleophile Conversely, when monomer units are joined together to form biopolymers such as proteins or glycogen, water is a product.
Water Molecules Exhibit a Slight butImportant Tendency to Dissociate: Since water can act both as an acid and as a base, its ionization may be represented as an intermolecular proton transfer that forms a hydronium ion (H3O+) and a hydroxide ion (OH−):
Total body ﬂuid is distributed between two compartments: Extracellular ﬂuid interstitial ﬂuid blood plasma Intracellular ﬂuid
Transcellular Fluid small compartment containing body fluids formed by the secretion of epithelial cell and is contained within epithelial lined spaces includes ﬂuid in the synovial, peritoneal, pericardial, and intraocular spaces, as well as the cerebrospinal ﬂuid specialized type of extracellular ﬂuid constitute about 1 to 2 liters.
In an average 70-kilogram adulthuman: Total body water is about 42 liters or 60 percent of the body weight This percentage can change depending on: Age Gender Degree of obesity.
Gender and Body Fat Women normally have more body fat than men They contain slightly less water than men in proportion to their body weight
Extracellular and Intracellular Fluid cell membrane is semipermeable, only water and small, noncharged molecules can move freely between interstitial and intracellular compartment. Ion can not cross easily. All kinds of ionic pump or channel on cell membrane determine the uneven distribution
Extracellular and Intracellular Fluid ICF and ECF are different in ionic composition
Intracellular Fluid About 28 of the 42 liters of ﬂuid in the body are inside the 75 trillion cells Constitutes about 40 percent of the total body weight Each cell contains its individual mixture of different constituents, but the concentrations are similar from one cell to another Composition of cell ﬂuids is remarkably similar even in different animals
Extracellular Fluid All the ﬂuids outside the cells Account for about 20 percent of the body weight, or about 14 liters in a normal 70-kilogram adult Two largest compartments: (1) interstitial ﬂuid more than three fourths of the extracellular ﬂuid, and (2) plasma almost one fourth of the extracellular ﬂuid, or about 3 liters *Plasma - non-cellular part of the blood - exchanges substances with the interstitial ﬂuid (pores of the capillary membranes) Constantly mixing plasma and interstitial ﬂuids have about the same composition except for proteins (  in the plasma)
Hypervolemia Term used for fluid overload, overhydrated and water excess It occurs when the body takes in more water than it excretes * Factors that cause Hypervolemia: Cardiac failure Renal failure (Kidney’s Damage) High sodium intake Over infusion of intravenous fluids.
Hypervolemia Infants are especially likely to develop overhydration (first month of life)* For adults, drinking too much water rarely causes overhydration when the bodys systems are working normally Brain- the organ most vulnerable to the effects of overhydration*
Hypervolemia Treatment Limit fluidintake In more serious cases, diuretics may be prescribed to increase urination Identifying and treating any underlying condition (such as impaired heart or kidney function) is a priority
Hypervolemia NOTE: The best advice is to drink when you are thirsty and to aim to drink 6-8 glasses, 2-3 litres per day; *Consider the differences in physical activity, climate and diet *Less fluid intake for lower intensity exercise in milder conditions and more for superior athletes competing at higher intensities in warmer environments
Hypervolemia• The best guidance is for individuals to remain adequately hydrated, but not overhydrated
The average adult body contains 40 liters of H2O. This amount, called total body water, remains fairly constant under normal circumstances.
Maintenance of Water Homeostasis It is a balancing act because the amount of water taken in must equal the amount of water lost.
Disturbance of Water Homeostasis Two categories Gain or loss of Extracellular fluid volume Gain or loss of solute In many instances disturbances of water and homeostasis involve imbalances of both volume and solute.
Mechanism of Fluid Balance Antidiuretic Hormone Thirst mechanism Aldosterone Sympathetic Nervous System
Electrolytes Cations Anions Electrolytes are not evenlydistributed within the body, and their uneven distribution allows many important metabolic reactions to occur
Purpose of Electrolytes Help control water balance and fluid distribution in the body Create an electrical gradient across cell membranes that is necessary for muscle contraction and nerve transmission Regulate the acidity (pH) of the blood Help regulate the level of oxygen in the blood Are involved in moving nutrients into cells and waste products out of cells
Specific Function Sodium- affects how much urine the kidney produces and is involved in the transmission of nerve impulses and muscle contraction Potassium- regulate fluid balance in cells, the transmission of nerve impulses, and in muscle contractions Calcium-build and maintain bones. It also plays a role in nerve impulse transmission and muscle contraction.
Specific Function Magnesium- involved in protein synthesis and cellular metabolism Chloride- involved in regulating blood pressure Phosphate- helps control the acidity level (pH) of the blood; also causes calcium to be deposited in bones
Saliva (Composition) Inorganic composition dependent on stimulus and rate of salivary flow Major components are Na+, K+, HCO3-, Ca++, Mg++, and Cl-.  of ions varies with the rate of secretion which is stimulated during the postprandial period. Organic constituents synthesized, stored, and secreted by the acinar cells Major products are amylase, lipase, glycoprotein (mucin, which forms mucus when hydrated), and lysozyme (attacks bacterial cell walls to limit colonization of bacteria in the mouth)
Saliva (Enzyme Content) 2 major types of protein secretion: Serous secretion contains ptyalin, an amylase enzyme for digesting starches and cleansing agent for the oral cavity *Saliva has a pH between 6.0 and 7.0 (favorable range for the digestive action of ptyalin) Mucus secretion contains mucin for lubricating and for surface protective purposes
Saliva (Enzyme Content) Parotid glands - serous type of secretion Submandibular and Sublingual glands - both seroussecretion and mucus Buccal glands - secrete only mucus *Aside from amylase, saliva has presence of other enzymessuch as maltase, catalase, lipase, urease, and protease.
Saliva (Control Mechanism) Salivary glands controlled mainly by parasympathetic nervous signals, from the superior and inferior salivatory nuclei in the brain stem Salivatory nuclei excited by both taste and tactile stimuli from the tongue, mouth and pharynx * salivation taste stimuli, especially sour taste (caused by acids), tactile stimuli, such as the presence of smooth objects in the mouth
Saliva (Control Mechanism) Salivation can be stimulated or inhibited by nervous signals arriving in the salivatory nuclei from CNS Appetite area of the brain located in proximity to the parasympathetic centers of the anterior hypothalamus, functions to in response to signals from the taste and smell areas of the cerebral cortex or amygdala.
Saliva (Control Mechanism) Saliva helps remove the irritating factor in the GIT (diluting or neutralizing the irritant substances) Sympathetic nerves from Superior cervical ganglia Salivary glands (slight increase in salivation) 2 factor - blood supply to the glands (nutrition) Parasympathetic nerve signals copious salivation moderately dilate the blood vessels increased salivatory gland nutrition
Saliva (Functions) Digestive function (enzymes) Moistens and lubricates food swallowed easily Holds the taste-producing substances brings in contact with the taste buds Dilutes salts, acids protecting the mucosa (teeth)
Saliva (Function on Oral Hygiene) Under basal awake conditions about 0.5 ml/ min of saliva (mucous type) *During sleep, secretion becomes very little. Saliva helps prevent deteriorative processes in several ways:1. Wash away pathogenic bacteria and food particles that provide their metabolic support2. Contains thiocyanate ions and lysozyme3. Contains protein antibodies that can destroy oral bacteria, including some that cause dental caries *In the absence of salivation, oral tissues often become ulcerated and infected caries of the teeth can become rampant.
Saliva (Tests) Saliva is easy to access and collection is non-invasive Used to identify individuals with disease (presence of biomarkers) and to monitor progress under treatment Viral infections such as human immunodeficiency virus (HIV), herpes, hepatitis C, and Epstein-Barr virus infection polymerase chain reaction (PCR) techniques Bacterial infections, such as Helicobacter pylori, can likewise be detected in saliva Monitoring drugs levels
Saliva (Clinical Disorders) *Abnormal production of the salivary glands can cause serious complications & adverse effects to salivary functions. Xerostomia (dry mouth) is caused by impaired salivary secretion Congenital or develop as part of an autoimmune process Decrease in secretion reduces pH in the oral cavity tooth decay and is associated with esophageal erosions difficulty swallowing.
Gastric Juice Stomach mucosa has two important types of tubular glands: (1) oxyntic glands (gastric glands) and (2) pyloric glands. Oxyntic (acid-forming) glands - secrete HCL, pepsinogen, intrinsic factor, and mucus 3 types of cells: (1) mucous neck cells (mucus); (2) peptic or chief cells (pepsinogen); and (3) parietal or oxyntic cells (hydrochloric acid and intrinsic factor) Pyloric glands - secrete mainly mucus (protection of the pyloric mucosa from the stomach acid) and hormone gastrin.
Composition: Inorganic Constituents Secretory rate Higher the concentration of ions [K+] is always higher in gastric juice than in plasma (prolonged vomiting may lead to hypokalemia). At high rates of secretion, gastric juice resembles an isotonic solution of HCl Gastric HCl converts pepsinogens to active pepsins and provides the acid pH at which pepsins are active
Composition: Inorganic Constituents Rate of gastric H+ secretion varies considerably among individuals Basal (unstimulated) rates of gastric H+ production 1 to 5 mEq/hr During maximal stimulation, HCl production from 6 to 40 mEq/hr Total number of parietal cells in the stomach partly responsible for the wide range in basal and stimulated rates of HCl secretion.
Gastric JuiceComposition: Organic Constituents Pepsin proteases secreted by the chief cells, active at pH 3 & below Pepsinogen the inactive proenzyme of pepsin Pepsinogens - contained in membrane-bound zymogen granules in the chief cells - converted to active pepsins by the cleavage of acid- labile linkages (the lower the pH, the more rapid the conversion)
Gastric JuiceEnzyme Content Pepsin Pepsinogen - no digestive activity, activated to pepsin when comes in contact with HCL Pepsin - proteolytic enzyme (optimum pH 1.8 to 3.5), above pH 5 almost no proteolytic activity (inactivated) Major products of pepsin action large peptide fragments and some free amino acids Gastric protein digestion important in peptides and amino acids generation stimulants for cholecystokinin release in the duodenum * Gastric peptides are therefore instrumental in the initiation of the pancreatic phase of protein digestion
Gastric JuiceEnzyme Content Gastric Lipase Initiating the digestion of lipids in the stomach. Converts triacylglycerols into fatty acids and diacylglycerols Initial hydrolysis important since some of the water-immiscible triacylglycerols are converted to products with both polar and non- polar groups (stable interface with the aqueous environment)
Gastric JuiceControl Mechanism: SECRETION Gastric secretion occur in three phases: (1) cephalic phase, (2) gastric phase, and (3) intestinal phase
Gastric JuiceControl Mechanism Cephalic phase Occurs even before food enters the stomach, especially while it is being eaten Results from the sight, smell, thought, or taste of food, and the greater the appetite, the more intense is the stimulation Neurogenic signals originate in the cerebral cortex and appetite centers of the amygdala and hypothalamus transmitted through the dorsal motor nuclei of the vagus vagus nerves to the stomach
Gastric JuiceControl Mechanism Gastric phase Once food enters the stomach excites the long vagovagal reﬂexes from the stomach to the brain and back to the stomach local enteric reﬂexes gastrin mechanism cause secretion of gastric juice during several hours while food remains in the stomach Accounts for about 70 per cent of the total gastric secretion associated with eating a meal (1500 ml).
Gastric JuiceControl Mechanism Intestinal phase Presence of food in the upper portion of the small intestine (duodenum) cause stomach secretion of small amounts of gastric juice (partly because of small amounts of gastrin released by the duodenal mucosa)
Gastric JuiceControl Mechanism: INHIBITION Affected by other post-stomach intestinal factors Presence of food in the small intestine reverse enterogastric reﬂex transmitted through the myenteric nervous system, extrinsic sympathetic and vagus nerves inhibits stomach secretion Can be initiated: distending the small bowel, presence of acid (upper intestine), presence of protein breakdown products, or by irritation of the mucosa This is part of the complex mechanism for slowing stomach emptying when the intestines are already ﬁlled.
Gastric JuiceControl Mechanism: INHIBITION Release of several intestinal hormones Secretin - important for control of pancreatic secretion but opposes stomach secretion Gastric inhibitory peptide, vasoactive intestinal polypeptide, and somatostatin - slight to moderate effects in inhibiting gastric secretion Functional purpose of inhibitory gastric secretion: to slow passage of chyme from the stomach when the small intestine is already ﬁlled or already overactive Enterogastric inhibitory reﬂexes plus inhibitory hormones also reduce stomach motility at the same time that they reduce gastric secretion
Gastric JuiceFunctions Gastric juice is characterized by the presence of hydrochloric acid and therefore a low pH less than 2 as well as the presence of proteases of the pepsin family Acid serves to kill off microorganisms and also to denature proteins *Denaturation makes proteins more susceptible to hydrolysis by proteases Serves in the initial hydrolysis of lipids with the help of gastric lipase enzyme
Gastric JuiceTests Qualitative tests include those for butyric acid, lactic acid, occult blood, bile and trypsin. Presence of the first two acids yeast or other microorganisms in the gastric secretions lack of hydrochloric acid If blood is present ulcers, hemorrhages and other pathologic states Either bile or trypsin evidence of regurgitation of intestinal contents Quantitative procedures total acidity contributed by HCL, organic acids and acid salts, neutralized or buffered by various constituents of the gastric juice and food
Gastric JuiceClinical Disorders Achlorhydia - absence of hydrochloric acid (pernicious anemia, gastric carcinoma) Hypoacidity - if HCL is not entirely absent but below normal (pregnancy, gastric carcinoma, gastritis and constipation, secondary anemia, and chronic debilitative diseases) Hyperacidity - acidity is elevated (duodenal ulcer and gallbladder disease) *It should be emphasized that the acidity cannot exceed a certain value (pH 0.87) since the parietal cells secrete a fluid of constant composition
Pancreatic Juice The pancreas, parallel to and beneath the stomach, is a large compound gland similar to the internal structure of salivary glands Exocrine secretion: combined product of enzymes and sodium bicarbonate secretions ﬂows through a long pancreatic duct joins the hepatic duct empties into the duodenum through the papilla of Vater, surrounded by the sphincter of Oddi. The pancreas also secretes insulin (not secreted by the same pancreatic tissue secreting pancreatic juice) Insulin is secreted directly into the blood—not into the intestine—by the islets of Langerhans that occur in islet patches throughout the pancreas
Pancreatic JuiceComposition Alkaline in nature with a pH of about 8. Large volumes of sodium bicarbonate solution are secreted by the small ductules and larger ducts leading from the acini Bicarbonate ions play an important role in neutralizing the acidity of the chyme emptied from the stomach into the duodenum. The pancreatic digestive enzymes are secreted by pancreatic acini
Pancreatic JuiceEnzyme Content Pancreatic secretion contains multiple enzymes for digesting all of the three major types of food: proteins, carbohydrates, and fats Proteins: Trypsin and chymotrypsin split whole and partially digested proteins into peptides of various sizes (do not cause release of individual amino acids) Carboxypolypeptidase split some peptides into individual amino acids completing digestion of some proteins all the way to the amino acid state
Pancreatic JuiceEnzyme Content Proteolytic digestive enzymes - inactive forms first: trypsinogen, chymotrypsinogen, and procarboxypolypeptidase Activated after secreted into the intestinal tract Trypsinogen activated by an enzyme called enterokinase (secreted by the intestinal mucosa when chyme comes in contact with it) or by previously activated trypsin Chymotrypsinogen and Procarboxypolypeptidase activated by trypsin to form chymotrypsin and carboxypolypeptidase
Pancreatic JuiceEnzyme Content Carbohydrates: Pancreatic amylase which hydrolyzes starches, glycogen, and most other carbohydrates (except cellulose) form mostly disaccharides and a few trisaccharides Fat: Pancreatic lipase hydrolyzing neutral fat into fatty acids and monoglycerides Cholesterol esterase hydrolysis of cholesterol esters Phospholipase splits fatty acids from phospholipids
Pancreatic JuiceControl Mechanism 3 Basic stimuli for pancreatic secretion Acetylcholine released from the parasympathetic vagus nerve endings and from other cholinergic nerves in the enteric nervous system Cholecystokinin secreted by the duodenal and upper jejunal mucosa when food enters the small intestine Secretin secreted by the duodenal and jejunal mucosa when highly acid food enters the small intestine
Pancreatic JuiceControl Mechanism Acetylcholine and cholecystokinin stimulate the acinar cells of the pancreas production of large quantities of pancreatic digestive enzymes (relatively small quantities of water and electrolytes) *Without water, most of the enzymes remain temporarily stored in the acini and ducts until more ﬂuid secretion comes along to wash them into the duodenum Secretin stimulates secretion of large quantities of water solution of sodium bicarbonate by the pancreatic ductal epithelium
Pancreatic JuiceControl Mechanism Pancreatic secretion occurs in three phases: (1) cephalic phase, (2) gastric phase and (3)intestinal phase Cephalic phase same nervous signals from the brain that cause secretion in the stomach cause acetylcholine release by the vagal nerve endings in the pancreas causes moderate amounts of enzymes to be secreted into the pancreatic acini *Accounting for about 20 per cent of the total secretion of pancreatic enzymes after a meal
Pancreatic JuiceFunctions Secretions from pancreas quantitatively the largest contributors to enzymatic digestion of food Provides additional important secretory products that are vital for normal digestive function including Water and bicarbonate ions neutralizing gastric acid so that the small intestinal lumen has a pH approaching 7.0. reduces injury to small intestinal mucosa by such acid acting in combination with pepsin *Pancreatic enzymes are inactivated by high levels of acidity
Pancreatic JuiceTests The fact that secretin stimulates the flow of pancreatic juice has been made use as a test of external pancreatic functions Double-lumen tube passed (longer end reaches the third portion of the duodenum and the shorter end remains in the stomach) continuous aspiration (-20 to 30 mmHg) prevents the overflow of gastric juice into the duodenum and sucks out both gastric juice and duodenal contents into separate containers secretin is injected intravenously after a basal flow has been obtained volume of flow and bicarbonate concentration is measured
Pancreatic JuiceTests Outpouring of pancreatic juice Under normal conditions, duodenal fluid lose its biliary color If the bile color remains a non-functioning gallbladder is indicated The total volume varies normally from 135 to 250 ml in 1 hour, and the bicarbonate, from 90 to 130 mEq *In pancreatitis with extensive destruction of parenchymal structures, there is usually a diminution in the volume of pancreatic juice and bicarbonate output
Pancreatic JuiceClinical DIsorders Damaged pancreas/ ducts blocked large quantities of pancreatic secretion become pooled in the damaged areas Pancreatitis enzymes secreted by pancreatic acinar cells become proteolytically activated before reaching appropriate site of action (small intestinal lumen) Pancreatic juice contains trypsin inhibitors reduce the risk of premature activation Trypsin can itself be degraded by other trypsin molecules *Specific mutation in trypsin renders it resistant to degradation by other trypsin molecules.
Pancreatic JuiceClinical DIsorders Pancreas reacts very sensitively to an impairment of the protein metabolism Decreased supply of proteins leads impairment of the endogenous stimulation of pancreas atrophy of acinous cells and ﬁbrosis of pancreas (e.g. long-term fasting) Decreased contents of pancreatic enzymes Decreased secretion of their proenzymes, insuﬃcient intraluminal activation or inactivation of enzymes *Celiac sprue secretions of lipase and trypsinogen are decreased trypsin deﬁciency deﬁciency of chymotrypsin and other enzymes which are activated from proenzymes by trypsin
BILE An alkaline, brownish- yellow or greenish-yellow fluid that is secreted by the liver, stored in the gallbladder, and discharged into the duodenum and aids in the emulsification, digestion, an d absorption of fats.
Bile Composition Water (85%), bile salts (10%),(Cholic, chenodeoxycholic, deoxycholi c, and lithocholic acid) mucus• pigments (3%), bile pigments e.g bilirubin glucuronide• fats (1%), such as Phospholipids (lecithin) , cholesterol• 0.7% inorganic salts
Bile Composition In concentrating process in gallbladder, water and large portions of electrolytes are reabsorbed by gallbladder mucosa Bile salts and lipid substances cholesterol and lecithin, are not reabsorbed The concentrated bile is composed of bile salts, cholesterol, lecithin, and bilirubin.
Bile Pigments Bilirubin and Biliverdin*Urobilin Urobilinogen Bilirubin Biliverdin(Brown) (colorless) (red) (green)
Bile Secretion Release of hormone Bile is subsequently secretin and CCK stored & concentrated in(chocystokinin) from the the gallbladder betweenduodenum increase bile meals secretion Bile is normally stored in gallbladder until needed in duodenum*
Bile Secretion After meal, bile enters the duodenum as a result of combined effects of : Gall bladder emptying Increased bile secretion by liver The amount of bile secreted per day ranges from 250 ml to 1 litre
Emptying of Gallbladder Gallbladder begins toempty, when food (fatty Gallbladder emptying is foods) reach the rhythmical contractions of duodenum about 30 the gallbladder* minutes after a meal* Effective emptying requires simultaneous relaxation of sphincter of oddi*
Bladder bile vs. Liver bile bile (%)Constituent Bladder bile (%) LiverWater 82.3-89.8 96.5-97.5Solids 10.2-17.7 2.5-3.5Bile Salts 5.7-10.8 0.9-1.8Mucus and Pigments 1.5-3.0 0.4-0.5Cholesterol and other 0.5-4.7 0.2-0.4lipidsInorganic Salts 0.6-1.1 0.7-0.8
ENTEROHEPATIC CIRCULATION About 95% of the salts secreted in bile are reabsorbed actively in the terminal ileum and re-used. Blood from the ileum flows directly to the hepatic portal vein and returns to the liver where the hepatocytes reabsorb the salts and return them to the bile ducts to be re- used, sometimes two to three times with each meal.
Function of Bile Juice* Bile acts as a surfactant , helping to emulsify the fats in the food. Bile salt anions have a hydrophilic side and a hydrophobic side, and therefore tend to aggregate around droplets of fat ( triglycerides and phosphiolipids ) to form micelles. The hydrophilic sides are positively charged due to the lecithin and other phospholipids that compose bile, and this charge prevents fat droplets coated with bile from re-aggregating into larger fat particles. Fat in micelles* form provide a large surface area for the action of the enzyme pancreatic lipase in the digestion of lipids.
Function of Bile Juice The alkaline bile has the function of neutralizing any excess stomach acid before it enters the ileum. Bile salts also act as bactericides, destroying many of the microbes that may be present in the food.
Preventing Metabolic Deficit Without the presence of bile salts in the intestinal tract, up to 40 percent of the ingested fats are lost into the feces, and the person often develops a metabolic deficit because of this nutrient loss.
Abnormalities associated with bile Gall stone- majority of gall stones are made up of cholesterol , (cholesterol tends to accrete into lumps in the gallbladder) Causes of gall stones; - Too much absorbtion of water from the bile . - Too much cholesterol in bile. - Inflammation of the epithelium.
Small intestine juice small intestine is where most chemical digestionand fluid absorption takes place Water and lipids are absorbed by passive diffusion throughout the small intestine. Sodium Bicarbonate is absorbed by active transport and glucose and amino acid co-transport Fructose is absorbed by facilitated diffusion
Intestinal Juice Intestinal juice is not as definite an entity as pancreatic juice or gastric juice because it varies at different levels of intestinal tract* Succus entericus is influenced by the hormone secretin Enterocrinin* stimulates mucosal glands
Intestinal Juice* leukocytes Epithelial cells Mucus Organic material 1.5% Solid 8.3 pH* eg. mucoproteinscomponents* and enzyme*
Feces Formation and CompositionFeces is the waste material passed out from the bowels through the anus. It is usually solid to semi- solid in consistency but can be hard in constipation or watery with diarrhea
Feces Formation Large amounts of water and electrolytes are absorbed in the first half of colon.* Water absorption transforms the fluid chyme into a mush-like consistency by the time it passes through the transverse colon. It solidifies further along its passage down the descending colon.
Composition of Feces About 75% of fecal weight is made up of water. The other 25% is composed of solid matter which contains : Undigested fiber and solidified components of digestive juices (30%) Bacteria (30%) Fat (10% to 20%) Inorganic matter (10% to 20%) Protein (2% to 3%)
Water and Electrolytes in the Colon Electrolytes(bicarbonate) are secreted by the wall of the large intestine into the lumen to neutralize any acidic byproducts of bacterial metabolism. Sodium and chloride are absorbed by the intestinal wall which creates a concentration gradient to facilitate water absorption.
Feces *Any extra fluid remain in the colon give a liquid consistency to the feces (loose stool). It also increases defecation frequency by triggering the local defecation reflex.
Feces The Bristol Stool Chartor Bristol Stool Scale is a medical aid designed to classify the form of human feces into seven categories The form of the stool depends on the time it spends in the colon.
Color of feces Feces usually has a brown color, ranging from a tan hue to a darker-brown color. Bilirubin is passed out in the bile and the action of bacteria and air in the gut breaks it down into stercobilin and urobilin, which gives stool its typical color.
CharacteristicsCharacteristics of urine under physiological conditions Characteristic Normal Quantity or Quality Volume 115 to 180 L /day (women) 130 t0 200 L / day (men) Color Amber Odor Not unpleasant (aromatic) Turbidity Clear and transparent pH 4.6 to 8.0
Color Odor Amber Does not have unpleasant Pigment: urochrome smell (aromatic) Other pigments: Other odors arise from food uroerythrin, uroporphyrins, Mercaptan-like odor after riboflavin eating asparagus Oil of wintergreen (has methyl salicylate) strong odor of evergreens
Turbidity pH Urine is clear and Varies from 4.6 to 8.0 transparent Protein diet gives rise to After standing for a long acidic pH oxidation of sulfur in sulfur containing time flocculent material amino acids into sulfuric (mucoprotein + acid nucleoprotein + epithelial Vegetable and fruit diet cells) separates gives rise to alkaline urine Urine samples taken right after eating meals are alklalinesecretion of H+ in the gastric juice
Ions in the urine Anions Cations The major anion is chloride Sodium and potassium are The amount is equal to the the major cations amount that was ingested Total excretion of Na+ varies In salt-poor diets, Cl- may be between 2.0 and 4.0 g/day absent K+ 1.5 to 2.0 g/day.
Nitrogenous organic compounds Component Amount Urea α the total nitrogen Urea 12 to 36 g per intake day (70-g Uric acid – end product of adult) purine metabolism; α purine Uric acid 0.7 g of uric intake acid per day Creatinine α muscle mass Creatinine 18 to 32 and Creatine predominant in (coefficient) 10 to 25 in children and pregnant women women Hippuric acid 0.7 g per day Hippuric acid - formed in the liver Indican 5 to 25 mg per Indican – potassium salt day
Glycosuria The normal glucose range is 10 to 20 mg per 100 mL or urine High amounts of sugars lead to glycosuria Glucosuria Pentosuria Lactosuria Galactosuria Fructosuria
Pentosuria Tests for pentosuria Occurs after eating unusual Benzidine + acetic acid + amounts of fruits and fruit urine mixture is heated juices then cooled rose-pink Idiopathic pentosuria – color (+ for pentose) occurs in the absence of L- (+) Benedict’s reagent xylulose dehydrogenase (a (-) baker’s yeast genetic disease) fermentation Xylulose is excreted in the urine
Lactosuria Tests for lactosuria Moderate amounts of (+) baker’s yeast lactose secretion is found in fermentation lactating women (+) mucic acid test Does not occur in pregnancy (-) Barfoed’s test Yields lactosazone
Fructosuria Tests for fructosuria Occurs in association with (+) Benedict’s reagent diabetes mellitus (+) baker’s yeast The site of difficulty is the fermentation liver (it’s where fructose is Yields glucosazone and stored as glycogen phenylhydrazine There is a deficiency in the enzyme fructokinase
Galactosuria Tests for galactosuria A consequence of reduction of alkaline copper galactosemia solutions Galactose is not detectable slight fermentation by in the urine prior to the baker’s yeast introduction of milk in the (+) Barfoed’s test diet (+) mucic acid test.
Proteinuria Tests for Proteinuria presence of proteins, most Nitric acid ring test especially albumin, in the Sulfosalicylic acid test – urine. degree of turbidity (+) Plasma proteins may pass Heat and acetic acid test – damaged renal epithelium ring of white turbidity (+)
Lipuria presence of large amounts of fat in the urine Urine is opalescent, turbid, or milky when voided The high blood fat (lipemia) that sometimes occurs in diabetes mellitus and lipoid nephrosis may lead to lipuria. Also in patients with fractures of the long bones with injury to the bone marrow
The lymphatic system maintains volume and pressure of the extracellular fluid returns excess water and dissolved substances from the interstitial fluid to the circulation Lymph is the fluid that is present in the lymphatic capillaries that drain the interstitial spaces.
Mechanism of lymph flow Travels down the pressure gradient. Muscular and respiratory pumps push the lymph forward via the action of semilunar valves. blood capillaries interstitial fluid lymph capillaries lymphatic veins lymph nodes lymph ducts and finally brachiocephalic veins and vena cavae.
Composition Lymph has a similar composition to blood plasma. The only difference is that it contains lower percentage of protein than blood plasma. Lymph albumin:globulin ratio > plasma albumin:globulin ratio Albumin is smaller than globulin it diffuses from plasma to lymph more readily than the latter. Fibrinogen, prothrombin, and leukocytes are present in lymph, too.
The composition of lymph varies with the state of digestion. After a meal, the fat content rises since more than half the fat absorbed goes by this route. The lymph becomes milky if the food contains much fat. Lymph has the same composition as the ISF Asit flows through the lymph nodes it comes in contact with blood, and tends to accumulate more cells (particularly, lymphocytes) and proteins.
Anedema that is caused by the blockage of lymph return
CSF functions in protecting the brain from sudden changes in pressures. Maintaining a stable environment removing waste products of the cerebral hemisphere.
Production of CSFchoroid plexus P P subarachnoid in the cisternae space ventriclesarachnoid villi venous blood
Characteristics of CSF under physiological conditions Characteristic Normal Quantity or Quality Volume 20 mL per day Color Colorless Specific gravity 1.004 to 1.008 Turbidity Clear pH 7.35 to 7.40
Component Concentration or ratio in CSF Glucose <4.5 mmol/L Lactate <2.1 mmol/L Proteins 0.15 to 0.45 g/LIgG (blood to CSF 500:1 ratio) IgG index 0.66
CSF is collected using lumbar puncture. The appearance and pressure of CSF are evaluated to know what kind of pathology is present in an individual.
Lange’s colloidalgold test Done by mixing decreasing dilutions of CSF with colloidal gold solution Normal CSF causes no change in the appearance of the orange-red colored • Paretic curve solution • Fluids with large globulin Fluids with pathologic content conditions produce color • Such curves are contained in change depending on the casese with general paralysis condition and dilutiona • Multiple sclerosis, lead poisoning with brain involvement
Luetic curve • Meningitic curve Fluids with limited globulin • Large amounts of globulin and and moderate albumin content globulin Certain forms of cerebro-spinal • All meningitic conditions show syphilis this kind of curve Also in brain tumor, poliomyelitis, and cerebral arteriosclerosis
Sperm & Semen (Contents) Fructose – this serves as the primary nutrient source of the sperm cells. Citric Acid – no definite function for the sperm. But is a basis for diagnosing acute and chronic prostatitis Fibrinogen – forms a clot when the semen is introduced to the reproductive tract, forms a clot for 15 mins then disintegrates to promote sperm motility.
Prostaglandins – makes the female reproductive tract more receptive to sperm movement and possibly causes backward, reverse peristaltic movement to propel the sperm towards the ovaries. (A sperm cell can reach the upper ends of the fallopian tube within 5 minutes) Profibrinolysin – is changed to fibrinolysin to dissolve the clot formed by the fibrinogen Calcium ion – enters the sperm when it is within the female reproductive tract thus giving the sperm a whiplash motion.
Contained in the acrosome Hyaluronidase – depolymerizes the hyaluronic acid polymers in the intercellular cement that holds the ovarian granulosa cells together. Proteolytic enzymes – digest proteins in the structural elements of tissue cells that still adhere to the ovum
The Prostate glands secretes a thin milky fluid that contains calcium, citrate ion, phosphate ion, a clotting enzyme and profibrinolysin. Alkalinic pH is to combat the Acidic pH of the vagina (pH of 3.5 to 4.0). The sperm becomes motile when the pH rises to about 6.0 to 6.5.
Control Mechanism Spermatogenesis Testosterone, secreted by the Leydig cells, essential for growth and division of the testicular germinal cells, first stage in forming sperm cells. Luteinizing hormone, stimulate the Leydig cells to release testosterone
Control Mechanism Follicle-stimulating hormone, stimulates the Sertoli cells; without this stimulation, the conversion of the spermatids to sperm will not occur. Estrogens, essential for spermiogenesis Growth hormone, promotes early division of the spermatogonia
Diagnostic Procedures Semen Allergy Test - Women with seminal plasma protein allergy (SPPA) have an immunologic response to human semen. The immunological mechanism of semen allergy is a type I hypersensitivity reactions.
Semen Analysis - A low sperm count is diagnosed as part of a semen analysis test. Sperm count is generally determined by examining semen under a microscope to see how many sperm appear within squares on a grid pattern. In some cases, a computer may be used to measure sperm count.
Semen analysis results Normal sperm densities range from 20 million to greater than 100 million sperm per milliliter of semen. You are considered to have a low sperm count if you have fewer than 20 million sperm per milliliter. Some men have no sperm in their semen at all. This is known as azoospermia
Transudate and Exudate(Introduction) There are various places in the body where fluids can accumulate. When fluids accumulate inside a cavity they are referred to as effusions. Effusions can occur in the pleural, pericardial, and peritoneal cavities of the body.
Transudate A transudate is a fluid that accumulates in cavities due to a malfunction of the filtering membranes of cavity linings. The fluid balance between the linings to become disrupted, which in turn leads to the buildup of fluid on one side of the membrane.
Exudate An exudate is a fluid that accumulates inside a cavity due to the presence of foreign materials such as bacteria, viruses, parasites, fungi, and tumor cells.
An exudate forms as a result of all these cells (both leukocytes and foreign material) and their metabolites filling the cavity.
How to differentiate transudatefrom exudate The major test used to differentiate between a transudate or an exudate is the concentration of total protein in a fluid. Transudates generally have total protein concentration less than 3.0 g/dL while exudates generally have a total protein greater than 3.0 g/dL.
Rivalta Test Rivalta test is used in order to differentiate a transudate from an exudate. A test tube is filled with distilled water and acetic acid is added. To this mixture one drop of the effusion to be tested is added. If the drop dissipates, the test is negative, indicating a transudate. If the drop precipitate, the test is positive, indicating an exudate.
Composition, Enzyme Content,Diagnostic Procedures: Lactate dehydrogenase Lactate dehydrogenase is an enzyme that is used by cells in metabolism and production of energy. When there is a large presence of cells and cell death such as in infection and inflammation the concentration of LDH in the area increases. Transudates will have LDH levels lower than 200 units/L while exudates will have LDH levels higher than 200 units/L.
Glucose and Amylase Decreased values in the concentration of glucose in an exudate can occur in bacterial infections, malignancies, rheumatoid arthritis, and tuberculosis. Concentrations of amylase can accumulate in an exudate in response to esophageal rupture, pancreatitis, and pancreatic cancer. No matter what chemistry testing is ordered on a fluid it is best to compare it in relation to serum levels to help assess whether a fluid is a transudate or an exudate
Leukocytes Transudates are generally clear and pale yellow in appearance as they are basically filtrates of plasma. These fluids contain very little cellular material. The leukocyte count is usually less than 1.0 X 109/L and the erythrocyte count is less than 100.0 X 109/L. The leukocytes that are present consist of monocytes and lymphocytes.
Exudates will generally appear cloudy or turbid. May appear yellow, brown, greenish, and even bloody. In some instances they may even be clotted due to the presence of fibrinogen The leukocyte count will usually be greater than 1.0 X 109/L and include neutrophils, lymphocytes, monocytes, eosinophils , and even basophils.
Function Supply nutrients to cartilage Act as lubricant to joint surfaces Carry away waste products
String Test- viscosity and clarity of the fluid can be examined. Tests Viscosity Clarity Normal Fluid When dropped from a Transparent & colorless- syringe, forms a string of light yellow greater than 10-15cm Inflammatory Fluid Low viscosity & flows like Cloudy & yellow/green H20 Non-inflammatory Fluid Clear & yellow Haemorrhagic fluid Cloudy & red/ red-brown *Normal synovial fluid is clear, pale, yellow, viscid, and does not clot
Chemical Tests Glucose- typically a bit lower than blood glucose levels. May be significantly lower with joint inflammation and infection. Protein- increased with bacterial infection Lactate dehydrogenase- increased level may be seen in rheumatoid arthritis, infectious arthritis, or gout. Uric acid- increased with gout
Microscopic Examination Total cell count- number of RBC’s and WBC. Increased WBC may be seen in infections such as gout & rheumatoid arthritis. WBC differential- determines the percentages of different types of WBC. ↑ neutrophil- seen with bacterial infections Greater that 2% eosinophil- suggest Lyme disease
Clinical Disorders Increase volume of the synovial fluid results in a variety of pathological process Non-inflammatory- Osteoarthritis, neuroarthropathy Inflammatory- rheumatoid arthritis, gout Septic- Bactericidal or fungal infection Haemorrhagic- Haemophilia or trauma
Enzyme content Tears secreted by the lacrimal gland contains the enzyme lysozyme which protects the cornea from infection by hydrolyzing the mucopeptide of the polysaccharide cell walls of many microorganisms.
Function Keeps the epithelium moist, thereby protecting the outer covering of the eye from damage due to dryness Creates a smooth optical surface on the front of the cornea Acts as the main supplier of oxygen & other nutrients to the cornea Carries waste products from the cornea Improves the quality of retinal image by smoothing out irregularities of the cellular surfaces Provide enzymes that destroy bacteria that can harm the eye
Schirmer Test Determines whether the eyes produces test enough to keep it moist This test is used when a person experiences very dry eyes or excessive watering of the eyes Performed by placing filter paper inside the lower lid of the eye & after a few minutes, the paper is removed & tested for moisture content. Flourescein eye drops are also used to test if tears can flow through the lacrimal ducts into the noseMore than 10 mm of moisture on the filter paper in 5 minutes is normal
Meniscometry Meniscometry is a minimally invasive test, which is particularly useful in assessing tear volume indirectly by measuring tear meniscus radius. The smaller the radius of curvature, the smaller the volume of tears present and the greater the capillary suction of fluid back into the menisci from the tear film.
Crocodile Tears An uncommon consequence of nerve regeneration subsequent to Bells palsy or other damage to the facial nerve in which efferent fibers from the superior salivary nucleus become improperly connected to nerve axons projecting to the lacrimal glands (tear ducts), causing one to shed tears (lacrimate) during salivation while smelling foods or eating.
Keratoconjunctivitis sicca Dry eye syndrome A relativelycommon condition, especially in older patients, that is characterized by inadequate tear film protection of the cornea because of either inadequate tear production or abnormal tear film constitution, which results in excessively fast evaporation or premature destruction of the tear film.
Human Breast Milk Composition & Function Fats Needed for the development of nervous system Insulation & component of cell membrane Lipid (grams/100ml milk) 4.38 FA (8C) trace PUFA 0.6(14%) Carbohydrates Provide energy (lactose is the predominant carb.) Carbohydrate (grams/100ml milk) lactose 7 oligosaccharides 0.5
Human Breast Milk Proteins Needed for protein synthesis for growth & development Composed mostly of whey (60%-80%) & casein Protein (grams/100 ml milk) 1.03 casein 0.3 a-lactalbumin 0.3 0.3 lactoferrin 0.2 IgA 0.1 IgG 0.001 0.001 lysozyme 0.05 serum albumin 0.05 ß-lactoglobulin - Contains lactoferrin that has bacteriostatic effect Contains antibodies, bifidus factor & digestive enzymes
Human Breast Milk Vitamins, minerals and other nutrients Contains a host of vitamins, minerals and other nutrients that support a host of different biochemical processes essential for lifeAsh (%) 0.20 Riboflavin (mg) 0.036Calcium (mg) 25-35 Niacin (mg) 0.177Iron (mg) 0.03 Pantothenic acid 0.223Magnesium (mg) 3 Vitamin B6 (mg) 10Phosphorous (mg) 13-16 Folacin (mg) 5Potassium (mg) 51 Vitamin B12 (mcg) 0.045Sodium (mg) 17 Vitamin A (mg) 58Zinc (mg) 0.17 Vitamin D (mg) 0.04Ascorbic acid (mg) 5 Vitamin E (mg) 0.34Thiamine (mg) 20 Vitamin C (mg) 4
Human Breast Milk Control mechanism Lactogenesis Transforms a mammary gland from its undifferentiated state to fully differentiated state in late pregnancy Stage I – occurs in mid-pregnancy ↑ in lactose, total protein and immunoglobulins ↓ in Na+ and Cl- Stage II – onset of milk secretion ↑ in lactose (further increase) and citrate
Human Breast Milk Control mechanism Lactation Abrupt ↓ in progesterone (removal of placenta) initiates milk secretion Prolactin Stimulates and maintains mammary glandular ductal growth and milk protein synthesis Oxytocin Promotes “milk let-down” reflex
Human Breast Milk Enzymes Lipase For fat breakdown Lactoperoxidase and lysozymes Bactericidal effect Other enzymes Transport moieties for other substances such as zinc, selenium & magnesium
Human Breast Milk Diseases associated with breastmilk Mastitis infection of the tissueof the breast that occurs most frequently during the time of breastfeeding causes pain, swelling, redness, and increased temperature of the breast occurs when bacteria, often from the babys mouth, enter a milk duct through a crack in the nipple Resolves through antibiotic medication
Human Breast Milk Subareolar abscess abscess or growth on the areolar gland, which is located in the breast under or below the areola cause is blockage of the small glands or ducts under the areola, with development of an infection under the skin Symptoms may occur as: Drainage and possible pus from lump beneath areolar area Fever General ill-feeling Swollen, tender lump beneath areolar area Treatment done with antibiotics
Human Breast Milk Duct ectasia syndrome occurs when a milk duct beneath nipple becomes dilated → duct walls thicken → duct fills with fluid → milk duct can then become blocked or clogged with a thick, sticky substance usually improves without treatment Antibiotics or surgery only instituted if signs/symptoms persist Tenderness in the nipple or surrounding breast tissue Redness A lump or thickening An inverted nipple
Human Breast Milk Breast engorgement can occur due to : sudden increased milk production that is common during the first days after the baby is delivered when the baby suddenly stops breastfeeding either because it is starting to eat solid foods or it is ill and has a poor appetite may also be caused when the mother does not nurse or pump the breast as much as usual alveoli become over-distended which can lead to the rupture of the milk-secreting cells can lead to cessation of milk production Severe engorgement of the breast can lead to breast infection
Human Breast Milk Current research for breast milk testing breast milk could be used to predict whether she is at risk of developing breast cancer, according to scientists Cells in the milk can easily be tested to see if they contain certain genes linked to the illness the DNA of the milk appears altered to those who have shown to be (+) to cancer as evidenced by their respective biopsies
Sweat Composition Highly variable, but Na+ and Cl- are the major electrolytes Mineral composition varies depending on the person’s activity sodium (0.9 gram/liter) potassium (0.2 g/l) calcium (0.015 g/l) magnesium (0.0013 g/l) zinc (0.4 milligrams/liter) copper (0.3–0.8 mg/l) iron (1 mg/l) chromium (0.1 mg/l) nickel (0.05 mg/l) lead (0.05 mg/l)
Sweat Function produced by glands in the deeper layer of the skin, the dermis main function is to control body temperature additional function of sweat is to help with gripping, by slightly moistening the palms
Sweat Enzyme Contains cysteine proteinase inhibitors inhibits papain which is known to cleave the Fc portion of immunoglobulins
Sweat Test Sweat Chloride Test common and simple test used to evaluate a patient who is suspected of having cystic fibrosis (CF) iontophoresis is employed to produce the necessary volume of sweat for the test normal sweat chloride = 10-35 mEqs/l sweat chloride value < 60 mEqs/l is indicative of CF Those having intermediate values are advised to have the test repeated on a periodic basis
Sweat Diseases associated with sweat Hyperhydrosis abnormal increased sweating In most cases, cause is unknown. In some cases, however, causes are: Obesity Hormonal changes associated with menopause (hot flushes) Illnesses associated with fever, such as infection or malaria An overactive thyroid gland (hyperthyroidism) Diabetes Certain medications
Sweat Idiopathic hyperhydrosis most common form of excessive sweating Cause is unknown can develop during childhood or later in life can affect any part of the body, but the palms and soles or the armpits are the most commonly affected occurs even during cool weather, but it is worse during warm weather and when a person is under emotional stress
Sweat Apocrine bromhidrosis most prevalent form of bromhidrosis bacterial decomposition of apocrine secretion contribute to its pathogenesis yields ammonia and short-chain fatty acids having strong odors
Sweat Eccrine bromhidrosis Happens when eccrine sweat softens keratin bacterial degradation of the keratin yields a foul smell Can be caused by ingestion of some foods or drugs like: Garlic Onion Curry Alcohol certain drugs (eg: penicillin, bromides)
Mechanisms of Detoxification• Primarily a function of the liver • Has 2 phases • Phase I • Directly neutralizes or converts a substrate into an intermediate one. • Phase II • Renders a non-toxic final product.
Phase I Involves a group of enzymes – cytochrome P450 family Uses O2 and NADH as a cofactor Employs varied reactions depending on the substrate to be detoxified Free radicals are produced in the process
Phase I Some substances that can cause overactivity of cytochrome P450 enzymes: Caffeine Alcohol Pesticides Sulfonamides Barbiturates
Phase I Phase I can directly neutralize some chemicals like: Caffeine
Phase I Overactivity of Phase I Leads to wide range of chemical tolerances Depletion of antioxidants Accumulation of intermediate substance Predisposition to liver cell damage Underactivity of Phase I Low tolerance to wide range of chemicals Longer detoxification time
Phase I Inducers of Phase I Foods from brassica family Cabbage, broccoli, Brussels sprouts Also stimulates Phase II detoxification pathway Contains Indole-3-carbinol, a powerful anti-cancer Citrus fruits Oranges, lemons and tangerines Contains limonene that is a strong inducer of Phase I & II
Phase I Inhibitors of Phase I Drugs benzodiazepines; antihistamines; cimetidine; ketoconazole Foods naringenin from grapefruit juice curcumin from turmeric capsaicin form chili pepper eugenol from clove oil quercetin from onions Aging
Phase II Involves conjugation reactions to neutralize toxins Essentially, there are 6 pathways Glutathione conjugation Amino acid conjugation Methylation Sulfation Acetylation Glucuronidation
Phase II Inducers of Phase II Sulfation: Cysteine, methionine, taurine Acetylation: most vegetables and fruits but especially cruciferous vegetables Glucuronidation: Fish oils, limonene-containing foods
Phase II Inhibitors of Phase II Glutathione conjugation: Selenium deficiency, vitamin B2 deficiency, glutathione deficiency, zinc deficiency Amino acid conjugation: Low protein diet Methylation: Folic acid or vitamin B12 deficiency
Phase II Inhibitors of Phase II Sulfation: Non-steroidal anti-inflammatory drugs (e.g. aspirin), tartrazine (yellow food dye), molybdenum Acetylation: Vitamin B2, B5, or C deficiency Glucuronidation: Aspirin, probenecid