Glycine and Serine Amino acid Presentation Transcript
GLYCINE AMINO ACIDDr. Mohammed Ellulu
What is the Glycine?2 Glycine is the smallest amino acids. Chemical formula is (NH2CH2COOH). Chemical structure: Colourless, sweet-tasting crystalline, hydrophilic or hydrophobic; due to its side chain is only one hydrogen atom.
Biosynthesis3 Glycine is non-essential A.A. Biosynthesized in the body from the A.A. Serine, which is in turn derived from 3-phosphoglycerate. The enzyme Serine hydroxymethyltransferase catalyses this transformation via the cofactor pyridoxal phosphate: Serine + tetrahydrofolate → Glycine + N5,N10- Methylene tetrahydrofolate + H2O
Biosynthesis in liver4 Catalyzed by glycine synthase (glycine cleavage enzyme), to get a reversible conversion. CO2 + NH4+ + N5,N10-Methylene tetrahydrofolate + NADH + H+→ Glycine + tetrahydrofolate + NAD+ Most proteins incorporate only small quantities of glycine. A notable exception is collagen, which contains about 35% glycine.
Absorption of Amino Acids5 Conceptually identical to monosaccharides. Luminal plasma membrane bears sodium- dependent amino acid transporters. Depends on the electrochemical gradient of sodium across the epithelium. Absorption of di/tripeptides in small intestinal epithelial cell by cotransport with H+ ions.
Metabolism and degradation6
Physiological actions of Glycine7 Create muscle tissue. Maintaining healthy central nervous and digestive systems. Provide protection via antioxidants from some types of cancer. Almost 1/3 of collagen, which keeps the skin and connective tissue firm and flexible. Glucogenic: supply body with glucose needed for energy. Help reduce the symptoms of prostatic hyperplasia in men.
8 Studies about Glycine
Amino acid promotes sleep (Ajinomoto,2004)9 A study of the brains of rats revealed that glycine accumulates in the pineal gland, a part of the brain associated with the rhythms of waking and sleeping. 3g of glycine has tested on a group of men with sleep problems. Taking the supplement within an hour before going to bed. The researchers said the subjects showed brainwave patterns associated with non-REM sleep sooner and slept longer than those who did not take the amino acid.
Cancer Addicted to Glycine (Jain et al, 2012)10 According to study, the amino acid glycine fuels fast-growing cancer cells. Glycine is consumed by cancer cells but is released by normal cells. Food diaries from 60 human cancer cell lines show that they all consume large amounts of the amino acid glycine when growing fast. The team used liquid chromatography coupled with mass spectrometry.
Collagen for Osteoarthritis (world congress, 2003)11 Joint diseases account for the majority of all chronic conditions in people aged 65 and older and osteoarthritis represents the single most prevalent age-related disease. Dr Hans-Konrad Selbmann, from the German University in Tuebingen, highlighted a study within 1991: Adam et al compared four treatments to reduce the patients levels of pain: 1- collagen hydrolysate. 2- gelatine. 3- gelatine-glycine-calcium-fluid combination. 4- albumin proteins. The study found that all three of the gelatine treatments were effective and significantly superior to the protein supplementation.
womens health (AHPA, 2005)12 New information on botanicals from American Herbal Products Association such as” red clover (Trifolium pratense) used for menopause, Chaste tree (Vitex agnus-castus) used to ease PMS, and soy (Glycine max), which is considered by some as an alternative to hormone replacement therapy.
Anti-carcinogenic factors13 Vitiva CEO, a special neutraceutical company in Slovenia, studied the rosemary-derived anti- oxidants, and yeast powder for the reduction of acrylamide in fried foods. Acrylamide is a carcinogen discovered by Swedish scientists in 2002. Yeast contains almost: L-cysteine, glycine and L- lysine.
References14 Ajinomoto Company (2004). Japanese amino acids supplier Ajinomoto is considering commercializing food supplements containing glycine, based on studies showing the amino acid could help people sleep better. William Reed Business Media SAS. NutraIngredients publications. Jain M, et al (2012). Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science 336:1040-1044. The 8th world congress of the Osteoarthritis Research Society International took place in Berlin from 12-15 October 2003. American Herbal Products Association (2005). The first International Symposium on the Role of Botanicals in Womens Health. The symposium will take place on 16 & 17 February at Rutgers University in New Brunswick, New Jersey.
SERINE AMINO ACID
What is Serine?16 Chemical formula HO2CCH(NH2)CH2OH. Chemical structure: One of the proteinogenic amino acids. By hydroxyl group, serine is classified as a polar amino acid.
Biosynthesis17 The biosynthesis of serine starts with the oxidation of 3-phosphoglycerate to 3-phosphohydroxy- pyruvate and NADH. Reductive amination of this ketone followed by hydrolysis gives serine. Serine hydroxymethyltransferase catalyzes conversions of L-serine to glycine and 5,6,7,8- tetrahydrofolate to 5,10-methylenetetrahydrofolate
Serine deficiency19 Serine deficiency is a rare, inherited, metabolic disorder that interferes with normal growth and development, particularly in the central nervous system. Unlike similar neurodevelopmental disorders, serine deficiency can be treated. Children with serine deficiency are born with an abnormally small head (called microcephaly).
Biological functions20 Metabolic participates in the biosynthesis of purines and pyrimidines. precursor to several amino acids including glycine and cysteine. precursor to numerous metabolites, including folate, which is the principal donor of one-carbon fragments in biosynthesis.
Biological functions21 Structural role catalytic function of many enzymes. occur in the active sites of chymotrypsin, trypsin. nerve gases and insecticides shown to act by combining with a residue of serine in the active site of acetylcholine esterase. Serine proteases are a common type of protease.
Biological functions22 Signalling D-Serine, synthesized in the brain by serine racemase from L-serine serves as a neurotransmitter. D-serine is a potent agonist at the glycine site of the NMDA-type glutamate receptor. For the receptor to open, glutamate and either glycine or D-serine must bind to it. D-serine is a more potent agonist at the glycine site on the NMDAR than glycine itself.
Biological functions23 Gustatory sensation Pure D-Serine is an off-white crystalline powder with dirty aroma. L-Serine is sweet with sour tastes at high concentration. D-Serine is sweet with an additional minor sour taste at medium and high concentrations.
Glycine and Serine amino acids share in metabolism pathway24
25 Studies about Serine
Cheese protect against IBD (Sprong et al, 2009)26 The study, published in the Journal of Dairy Science, suggests that dietary cheese whey protein could reduce symptoms and markers of inflammatory bowel disease (IBD), after it was found to reduce gene expression of inflammation markers and diminish the clinical symptoms of IBD such as diarrhea and fecal blood loss in an animal model. The study recommended that whey protein rich in: threonine, serine ,cysteine.
Soy and Casein decrease muscle27 protein breakdown (Luiking et al, 2010) The study involved 22 healthy 22 year olds randomly assigned to receive moderate-nitrogen and carbohydrate casein and soy meals. Researchers from the University of Arkansas for Medical Sciences, Maastricht University….US. The synthesis of muscle protein between the groups, did not suggest differences in changes in soy and casein, as they were rich in glutamate, glutamine, serine, histidine, isoleucine and branched chain amino acids.
Energy abounds in soft drinks market28 Energy drinks in the UK the Jordan Grand Prix brand has jumped on the bandwagon with the creation of the EJ-10 Liquid Performance Drink, named after Jordan Grand Prixs founder Eddie Jordan and the 10 active ingredients in the drink. Jordan EJ-10 is a hypotonic drink targeting drivers. It contains phosphatidyl serine, maltodextrins and isolated proteins, but no caffeine or taurine. Mintels Global New Products Database: www.gnpd.com
Chemi Nutraceuticals29 Chemi Nutraceuticals is the new US branch of parent company C hemi, a privately held pharmaceutical and nutraceutical company based in Milan, Italy. Chemi, with cGMP certified manufacturing facilities in Italy and Brazil, is best known in the US nutritional arena for its introduction of phosphatidyl serine (PS), the popular dietary supplement used to enhance learning, memory, and concentration.
References30 Sprong RC , Schonewille AJ and van der Meer R (2009). Cheese whey protein may help to reduce genetic markers and symptoms associated with inflammatory bowel disease, according to a new study in rats. Journal of Dairy Science. 93: 1364– 1371. Luiking YC, Engelen MPKJ, Soeters PB, Boirie Y and Deutz NEP (2010). Protein from soy or casein both benefit the synthesis of muscle protein at the same rate. Clinical Nutrition. doi: 10.1016.