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Notch–RBP-J signaling regulates the transcription factor IRF8 to promote inflammatory macrophage polarization
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Notch–RBP-J signaling regulates the transcription factor IRF8 to promote inflammatory macrophage polarization

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Notch–RBP-J signaling regulates the transcription factor IRF8 to promote inflammatory macrophage polarization

Notch–RBP-J signaling regulates the transcription factor IRF8 to promote inflammatory macrophage polarization

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  • 1. nature immunology ArticlesNotch–RBP-J signaling regulates the transcription factor IRF8 to promote inflammatory macrophage polarization. Haixia Xu, Jimmy Zhu,Sinead Smith, Julia Foldi, Baohong Zhao, Allen Y Chung, Hasina Outtz, JanKitajewski, Chao Shi, Silvio Weber, Paul Saftig, Yueming Li, Keiko Ozato,Carl P Blobel, Lionel B Ivashkiv & Xiaoyu Hu Mohamed Antar Aziz Molecular biology lab. 2012310880
  • 2. Notch–RBP-J signaling regulates the transcription factor IRF8 to promote inflammatory macrophage polarization INTRODUCTION RESULTS DISCUSSION
  • 3. INTRODUCTION* Macrophages serve essential sentinel and effector functions in innate immunity and the transition to adaptive immunity. *the functional phenotype of macrophages is regulated by functional phenotype transcription factors that define alternative activation states. M2 macrophages have lower expression of M1 macrophages are characterized by the production of inflammatory mediators and have key roles in inflammatory mediators, (IL-12) (iNOS), in response to wound healing, host defense against helminthes microbial product–mediated activation of Toll-like and the resolution of inflammation receptors (TLRs) and cytokines such (IFN-γ)
  • 4. *Members of the interferon-regulatory factor (IRF) family of proteins aretranscriptional regulators of macrophage polarization, with IRF5 and IRF4 associated with polarization to the M1 state and M2 state, respectively*Translation-control mechanisms often target the process of translation initiation, during which the recruitment and assembly of translation- initiation factors, including the main cap-binding protein eIF4E.* IRF8 is induced by IFN-γ and contributes to the induction of several genes, including Ifnb1 (which encodes IFN-β)4, Il12b (which encodes the p40 subunit of IL-12 (IL-12p40))5, Il12a (which encodes the p35 subunit of IL- 12)6 and Nos2 (which encodes iNOS), in response to stimulation via TLRs.
  • 5. MAPK NF-kB IRFERK1/2 JNK P38
  • 6. *The Notch signaling pathway regulates the differentiation, proliferation, survival and development of cells ADAM γ- secretase RBP-J NUCLEUS
  • 7. RESULTS*RBP-J controls the expression of prototypical M1 macrophage–associated genes.
  • 8. Together these results showed that RBP-J was essential for the expression ofgenes characteristic of the core M1 macrophage response in vitro and for the manifestation of key myeloid effector functions in vivo.
  • 9. RBP-J has a key role in signal transduction via the canonical Notch pathway.
  • 10. which Notch receptors were responsible for the activation of the M1 macrophage– associated genes.
  • 11. Collectively, these results indicated that the Notch1–ADAM10–γ-secretase–RBP-J axis regulated the expression of M1 macrophage–associated genes.
  • 12. RBP-J controls IRF8 expression and functionThe recruitment of IRF8 to its target-gene promoters is necessary for the binding of RNA polymerase II and subsequent transcriptional activation
  • 13. RBP-J regulated the expression of M1 macrophage–associatedgenes through IRF8.
  • 14. The rapid synthesis of IRF8 protein induced downstream ofTLR4 signaling was dependent on RBP-J.
  • 15. RBP-J controls activation of the MNK1-eIF4E axis
  • 16. RBP-J targets IRAK2 upstream of MNK1-eIF4E
  • 17. MNK1-eIF4E controls the TLR4-induced synthesis of IRF8 protein
  • 18. DISCUSSION• We found that RBP-J, the main nuclear transducer of signaling via Notch receptors, augmented Toll-like receptor 4 (TLR4)-induced expression of key mediators of classically activated M1 macrophages and thus of innate immune responses to Listeria monocytogenes.• Notch–RBP-J signaling controlled expression of the transcription factor IRF8 that induced downstream M1 macrophage–associated genes.• RBP-J promoted the synthesis of IRF8 protein by selectively augmenting kinase IRAK2– dependent signaling via TLR4 to the kinase MNK1 and downstream translation-initiation control through eIF4E.• There is signaling network in which signaling via Notch–RBP-J and TLRs is integrated at the level of synthesis of IRF8 protein and identify a mechanism by which heterologous signaling pathways can regulate the TLR-induced inflammatory polarization of macrophages.