• Save
Smart strip don: a new, fast, reliable lateral flow for vomitoxin
Upcoming SlideShare
Loading in...5
×
 

Smart strip don: a new, fast, reliable lateral flow for vomitoxin

on

  • 123 views

For acceptance controls, for low throughput analysis, for outdoor, on-field screening, Smart Strip DON is Tecna NEW, quick, reliable lateral flow device for the qualitative and quantitative detection ...

For acceptance controls, for low throughput analysis, for outdoor, on-field screening, Smart Strip DON is Tecna NEW, quick, reliable lateral flow device for the qualitative and quantitative detection of deoxynivalenol (vomitoxin) in cereals. Easy to manage, easy and fast to perform, adaptable to different needs, Smart Strip DON is the right tool to analyse such mycotoxin! For further information visit Tecna website www.tecnalab.com or contact us at export@tecnalab.com!

Statistics

Views

Total Views
123
Views on SlideShare
120
Embed Views
3

Actions

Likes
0
Downloads
1
Comments
0

1 Embed 3

https://www.linkedin.com 3

Accessibility

Categories

Upload Details

Uploaded via as Microsoft PowerPoint

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

Smart strip don: a new, fast, reliable lateral flow for vomitoxin Smart strip don: a new, fast, reliable lateral flow for vomitoxin Presentation Transcript

  • Development of a quantitative lateral-flow immunoassay for the detection of deoxynivalenol in cereals
  • About deoxynivalenol 2 •Fusarium spp. (mainly F. graminearum and F. culmorum) WHO •15 – 30 °C, water activity > 0.85 WHEN •Cereals (mainly wheat, durum wheat, barley, maize and their derivatives) WHERE •Human gastroenteritis with nausea, diarrhea and vomiting. •The toxicity in animals is reflected by feed refusal and growth retardation. •All animal species are susceptible (pigs > mice > rats > poultry ≈ ruminants). WHAT
  • Screening techniques 3 Fastness–Easeofuse–suitabilityfor acceptancecontrols Sensitivity–Characterizationofanayltes–Skill requirement
  • Lateral flow immunoassay 4 Lateral flow immunoassays are tests based on a kind of immunochromatography. All reagents are gereally inside the device and this makes the test suitable to be perfromed by almost everyone, everywhere. As the sample is loaded on the sample pad, it migrates toward the conjugate pad, where it dissolves the tracer, an anti-deoxynivalenol antibody colloidal gold conjugate. Thanks to capillarity, the liquid flow migrates through the membrane, reaching the immunoreactive lines. On the Test (T) line a conjugate is absorbed. This is a competitor to the free deoxynivalenol molecules eventually present in the sample. The other line is the Control (C) line, where anti-species secondary antibodies are adsorbed and which represent the positive control of the assay. In case the control line would not be coloured, at the end of the assay time, the test is to be considered invalid.
  • Why? 5  Lateral flow devices are hence characterized by fastness and ease of use.  They don’t need many dedicated laboratory equipments.  They are a good option for factories, plants and small industies with low throughput analysis. Is now avaliable on the market for the QUALITATIVE & QUANTITATIVE detection of deoxynivalenol (vomitoxin) in cereals
  • The test kit Kit insert Dilution buffer Pouches containing the strips Barcode Businesscard 6
  • Product presentation  Assay principle: competitive lateral flow immunoassay for the qualitative and quantitative detection of deoxynivalenol  Applications: cereals  Measuring range: 500 - 12500 ppb  Assay time: 10 minutes for quantitative results, 3 minutes for qualitative results  Sample preparation: water extraction, centrifugation, dilution 7
  • Sample preparation Weight 5 g of ground sample and add deionized or distilled water Shake thoroughly for 3 minutes Allow sample to settle down Centrifuge the upper phase 1 minute Dilute with the provided dilution buffer 8
  • Assay implementation 9 Pipet the diluted extract in the sample well Wait for 10 minutes Put the developed cassettes in the reader
  • The readers 10 LAB LFD READER SMART READER • Multiplex laboratory reader • Ideal for testing more samples together • Ideal for using different assays per time • Up to 5 different assays can be read at a time • Portable, stand-alone reader, no computer necessary. • Up to 2 different assays can be read at a time • Suitable for acceptance controls: distinguish compliant and non compliant cereal samples in 3 minutes only.
  • 11  The quantification depends on the ratio of the colour intensity of the TEST LINE (T) and the CONTROL LINE (C).  The T/C value is interpolated by the reader onto a MASTER- calibration curve that is uploaded with a barcode businesscard for every kit batch.  The Control line must be developed and homogeneous, otherwise the test is invalid  Both readers are able to verify wether the assay is compliant or not Results achievement
  • Qualitative screening Acceptance control must be rapid Check whether your sample is < 750 ppb or > 1000 ppb in 3 minutes The Smart Reader is suitable to pre-view results. Then you can wait 7 minutes more and obtain the concentraiton. Validation  Specificity 100%: no compliant samples are detected as positive  Sensitivity 100%: all > 500 ppb samples are detected as positive. 12
  • Remarks: why SMART STRIP DON 13 # Plastic cassettes provide high manageability of the devices. A lot of room is available to write the name or the code of each sample, thus ensuring its traceability. # The sample preparation is drammatically easy: no solvent, incubator, vortex is necessary. # There are no reagents to prepare and mix, no strip to cut or dry: the sample extract is ready to be dropped on the sample pad. The reaction is incubated 10 minutes at room temperature. # The end user is free to choose the most fit-for-purpose reader within two alternative options: while the LAB LFD READER allows to save time when more samples are run together, the SMART READER is the right tool to accelerate the acceptance control, allowing to obtain a first qualitative information about deoxynivalenol content in 3 minutes only, with a sensitive cut-off at 500 ppb!
  • Appendix 14  Other analytical methods can be chosen to have different performances  ELISAs are now available in different formats 1. Master-curve calibrated, the most cost effective layout, ideal for those implementing a few samples 2. Fast, single replicate layout: 15 – 20 minutes assays 3. Double replicates, to have higher accuracy, reliability and precision
  • Contacts 15