How well do you know the Krebs cycle? Let me explain these steps.
In cellular respiration 2 pyruvate are created but only 1 is needed to go through the Krebs cycle, thus it goes around twice so remember what you end up with it doubled. Takes place in the mitochondria.
1) AcetylCoA adds two carbon fragment so that it can oxaloacetate to make citrate.
2) Citrate is converted to isocitrate by removing one water molecule to add another.
3) citrate loses a CO2 molecule, where the resulting compound would than be oxidized making NAD to NADH. Making 1 NADH.
4) Another CO2 is lost which than cause NAD to become NADH, than the left over molecule attaches to coenzyme A, but is unstable. Making 1 NADH.
5) CoA is than displaced by a phosphate group which is formed into GDP, which makes GTP, than ADP, which formed ATP. Making 1 ATP.
6) 2 hydrogen's are transformed into FAD to form FADH2. producing 1 FADH2.
7) the addition of water molecules cause the bonds in substrate to rearrange.
8) The substrate is than oxidized making NAD to NADH, than regenerating oxaloacetate. Making 1 NADH.