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  • Mixtures = analyte
  • Adsorbent = atoms that accumulate on the surface of the material

Transcript

  • 1. CHROMATOGRAPHY Nur Eliya Jasmi Nur Amalina Mohd Noor Nur Hamizah Salim Nur Azirah Norzaini Tan
  • 2. What is CHROMATOGRAPHY ? colour to write
  • 3. WHAT IS CHROMATOGRAPHY ?
    • A technique for separating mixtures into their components in order to analyze , identify , and purify the mixture or components.
  • 4. DEFINITION
    • Analyze : To examine the mixture or structure or something especially by separating it into its parts.
    • Purify : To make something pure by removing substances that are not wanted out of another substances that contains it.
    • Identify : To recognize something and to determine the identity of mixture.
  • 5. CHROMATOGRAM
    • The visual output of the chromatograph. Different patterns on the chromatogram correspond to different components of the separated mixture
  • 6. CHROMATOGRAM x axis – Retention time y axis – Solute concentration that leaving a chromatographic column. Retention time : Time taken for the analyte to pass through the system under set conditions.
  • 7. PHASE IN CHROMATOGRAPHY ?
  • 8. STATIONARY PHASE ADSORBENT : atoms that accumulate on the surface of the material
  • 9. TYPE OF STATIONARY PHASE
  • 10. MOBILE PHASE ELUENT : solvent that carry the analyte in elution.
  • 11. MOBILE PHASE SUPER CRITICAL FLUID: mobile phase is a fluid above and close to its critical temperature and pressure.
  • 12. COMPARISON OF THE PHASE MOBILE PHASE GAS GAS CROMATOGRAPHY (GC) LIQUID LIQUID CHROMATOGRAPHY (LC) STATIONARY PHASE LIQIUD GAS-LIQUID CHROMATOGRAPHY (GLC) SOLID GAS-SOLID CHROMATOGRAPHY (GSC) LIQUID LIQUID-LIQUID CHROMATOGRAPHY (LLC) SOLID LIQUID-SOLID CHROMATOGRAPHY (LSC)
  • 13. ANALOGY…
  • 14. R F VALUE It is the distance travelled by the sample or analyte divided by distance travelled by the solvent front in chromatography.
  • 15. EXAMPLE OF R F VALUE
  • 16. MECHANISME OF CHROMATOGRAPHY
  • 17. ADSORPTION
    • Surface phenomenon where interaction takes place only on the surface of one substance.
  • 18. ABSORPTION <> ADSORPTION
  • 19. ABSORPTION <> ADSORPTION
  • 20. ELUTION
    • The process of extracting a substance that is adsorbed to another by washing it with a solvent.  
    •    
  • 21. ELUTION
  • 22. PARTITION S aq  <==> S org
  • 23. PARTITION
  • 24. SUMMARIZE OF CHROMATOGRAPHY
  • 25. IF COLOURLESS ???
  • 26. ULTRAVIOLET DETECTOR
  • 27. ULTRAVIOLET DETECTOR MECHANISM
  • 28. THE END