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Chromatography

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  • Mixtures = analyte
  • Adsorbent = atoms that accumulate on the surface of the material
  • Transcript

    • 1. CHROMATOGRAPHY Nur Eliya Jasmi Nur Amalina Mohd Noor Nur Hamizah Salim Nur Azirah Norzaini Tan
    • 2. What is CHROMATOGRAPHY ? colour to write
    • 3. WHAT IS CHROMATOGRAPHY ?
      • A technique for separating mixtures into their components in order to analyze , identify , and purify the mixture or components.
    • 4. DEFINITION
      • Analyze : To examine the mixture or structure or something especially by separating it into its parts.
      • Purify : To make something pure by removing substances that are not wanted out of another substances that contains it.
      • Identify : To recognize something and to determine the identity of mixture.
    • 5. CHROMATOGRAM
      • The visual output of the chromatograph. Different patterns on the chromatogram correspond to different components of the separated mixture
    • 6. CHROMATOGRAM x axis – Retention time y axis – Solute concentration that leaving a chromatographic column. Retention time : Time taken for the analyte to pass through the system under set conditions.
    • 7. PHASE IN CHROMATOGRAPHY ?
    • 8. STATIONARY PHASE ADSORBENT : atoms that accumulate on the surface of the material
    • 9. TYPE OF STATIONARY PHASE
    • 10. MOBILE PHASE ELUENT : solvent that carry the analyte in elution.
    • 11. MOBILE PHASE SUPER CRITICAL FLUID: mobile phase is a fluid above and close to its critical temperature and pressure.
    • 12. COMPARISON OF THE PHASE MOBILE PHASE GAS GAS CROMATOGRAPHY (GC) LIQUID LIQUID CHROMATOGRAPHY (LC) STATIONARY PHASE LIQIUD GAS-LIQUID CHROMATOGRAPHY (GLC) SOLID GAS-SOLID CHROMATOGRAPHY (GSC) LIQUID LIQUID-LIQUID CHROMATOGRAPHY (LLC) SOLID LIQUID-SOLID CHROMATOGRAPHY (LSC)
    • 13. ANALOGY…
    • 14. R F VALUE It is the distance travelled by the sample or analyte divided by distance travelled by the solvent front in chromatography.
    • 15. EXAMPLE OF R F VALUE
    • 16. MECHANISME OF CHROMATOGRAPHY
    • 17. ADSORPTION
      • Surface phenomenon where interaction takes place only on the surface of one substance.
    • 18. ABSORPTION <> ADSORPTION
    • 19. ABSORPTION <> ADSORPTION
    • 20. ELUTION
      • The process of extracting a substance that is adsorbed to another by washing it with a solvent.  
      •    
    • 21. ELUTION
    • 22. PARTITION S aq  <==> S org
    • 23. PARTITION
    • 24. SUMMARIZE OF CHROMATOGRAPHY
    • 25. IF COLOURLESS ???
    • 26. ULTRAVIOLET DETECTOR
    • 27. ULTRAVIOLET DETECTOR MECHANISM
    • 28. THE END

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