Webinar: Elisa data analysis made easy with MasterPlex ReaderFit

Live Webinar: Elisa Data Analysis with MasterPlex ReaderFit Robert Lynde and Allen Liu September 10 th , 2009

Overview 1) File Import Wizard 2) Automatic Detection of Outliers 3) Marking Wells and Grouping of Replicates 4) Entering Standard Information 5) Best Fit and Best Fit Parameters 6) Plotting Unknown Data Points on the Standard Curve 7) WYSIWYG Data Table 8) Chart Customization 9) Templates 10) Custom Reports 11) Virtual Plate

File Import Wizard
The File Import Wizard can recognize and import text & Excel files with raw data 2 different formats:
Plate
List
10/22/09

Automatically Detect Outliers
Mark and select thresholds based on:
Raw signal (e.g. absorbance, luminescence, fluorescence, RLU etc.)
Concentration
Standard Deviation
Options are located under Plate Preferences->Plate tab

Marking Wells & Grouping of Replicates
There are 4 different well types:
Background
Standard
Auto Fill Feature – works really nicely for serially diluted standards
Manual Input – one can also type in the values
Unknown
Control
The following stats will be calculated based on your replicates (for both raw signal and Concentration):
Standard Deviation
% CV
Mean
Bonus Tips
Group replicates with the group stamping feature
Pasting sample names
Batch dilution factor for unknowns

Best Fit and Best Fit Parameters
Leave it to the software to do all the hard work for you with the Best Fit feature
Optimize based on your chosen parameter with the default being RMSE
Optimize the Best Fit based on:
Root mean square error (RMSE)
R-square
Standard deviation of % Recovery
Options are located under Plate Preferences->Plate tab

Plotting Unknown Data Points on the Standard Curve
Get a quick visual overview of where your unknown data points fall on the standard curves
Useful for determining whether your standard range is sufficient
Useful for determining whether the unknown data points fall onto to the linear or dynamic range
Bonus Tip – Zoom in and out on standard curve chart using the Shift and Alt keys

WYSIWYG Data Table
Customize the Data Table by:
Adding/Removing data columns
Filtering data
Sorting data
And then simply export to many different output formats including Excel

Chart Customization
Easily change and configure many chart properties such as:
Legends
Axes
Labels
General Appearance
Bonus Tip – Sample order on X axis

Templates
Save all your work into templates and reload them in seconds
Templates are available for:
Plate layout and standard information
Data Table
Charts

Custom Reports
The Data Table is extremely flexible but it is not the one-size-fits-all solution
This is where the Custom Report feature leaves off
One free custom report with purchase and additional reports available as a service

Virtual Plate
Easily merge different plate files together with the Virtual Plate feature
Handy for:
Using standards on one plate for another plate
Stitching together 2 incomplete files from the same run
When 96 wells just is not enough for your assay

Resources
MiraiBio offers the following resources:
Community: http://www.miraibio.com/community
Blog : http://www.miraibio.com/blog
Knowledge Base: http://www.miraibio.com/knowledge-base.html
Store: http://store.miraibio.com
Live Chat: located on homepage and most product pages
Support email: [email_address]
Sales: [email_address]
10/22/09

Webinar: Elisa data analysis made easy with MasterPlex ReaderFit

by MiraiBio Group of Hitachi Solutions America, Ltd. on Oct 22, 2009

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