Improving your results with MasterPlex QT 2010
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Improving your results with MasterPlex QT 2010

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In this webinar, you will learn how to improve the speed & integrity of your results using MasterPlex QT with an emphasis on curve-fitting....

In this webinar, you will learn how to improve the speed & integrity of your results using MasterPlex QT with an emphasis on curve-fitting.

Topics will include:

1) A close up look at the 4-PL and 5-PL non-linear regression models

2) Heteroscedasticity & Weighting

3) Ensuring the quality of your data with the QC Manager

4) How to use time-saving features such as templates & Best Fit

5) New features in MasterPlex QT 2010

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  • Most popular model equation in the Luminex world Recommended on most manuals for commercial kits
  • 4PL curve with 3 replicates 4 & 5PL assume equal standard deviations for all standard groups This phenomenon is called heteroscedasticity where we see nonconstant variance To account for heteroscedastic data such as this

Improving your results with MasterPlex QT 2010 Presentation Transcript

  • 1. Presenters: Robert Lynde Deputy Director Sales Operations & Strategic Alliances Manager Allen Liu Supervisor Technical Support & Project Management Webinar: Improving your results with MasterPlex QT July 29, 2010 This presentation accompanies the free webinar recording at: https://www1.gotomeeting.com/register/351125992
  • 2.
    • A close up look at the 4-PL and 5-PL model equations
    • Heteroscedasticity & Weighting
    • BONUS: What’s New in MasterPlex QT 2010
    • Ensuring the quality of your data with the Quality Control Manager
    • How to use time-saving features such as templates & Best Fit
    Topics
  • 3. 4 Parameter Logistic (4PL)
    • F(x) = ((A-D)/(1+((x/C)^B))) + D
    • Nonlinear regression
    • Monotonic
      • e.g. For every MFI, there is only one concentration
    • Symmetrical around the midpoint or inflection point
  • 4. 4 Parameter Logistic (4PL) A = minimum asymptote D = maximum asymptote C = inflection point B = slope factor/steepness of curve < low standard OR lowest extrapolated value > high standard OR highest extrapolated value
  • 5. Caution on Extrapolation tiny change in MFI = HUGE change in concentration
  • 6. 5 Parameter Logistic (5PL)
    • F(x) = A + (D/(1+(X/C)^B)^ E )
    • Nonlinear regression
    • Monotonic
    • Key difference = asymmetry
    • E = asymmetry factor
    • E = 1 = 4PL model equation
  • 7. 5 Parameter Logistic (5PL)
  • 8. Assumptions of the 4PL & 5PL
    • 2 assumptions about how the data are scattered around the curve…
      • The scatter follows a Gaussian or “normal” distribution
      • The standard deviation of the scatter (of the response values or MFI) is the same for all values of x (or concentrations in our case)
  • 9. Heteroscedasticity
  • 10. Weighting
    • 1/Y^2 (Relative weighting) – Most commonly used
    • 1/Y – Most effective when the scatter follows a Poisson distribution
    • Mainly affects points on the lower portion of the curve
    • Test by doing before/after on the % Recovery
  • 11.
      • Expanded Platform Support
        • Meso Scale Discovery (MSD) platform compatibility
      • Improved Data Integrity
        • Built-in Quality Control Manager
        • Added new Upper Limit of Detection calculation for QC Manager
      • Further Time-saving
        • Auto calculation for plate templates
      • Dose Response Analysis
        • EC or IC50 calculation
        • EC or IC(anything) calculation
      • Enhanced Reporting
        • Merge group wells in the Data Table
        • Customizable standard curve chart titles & x/y-axes
    What’s new for MasterPlex QT 2010?
  • 12.
    • Free 14-Day Trial: http://www.MiraiBio.com/QT
    • Support: [email_address]
    • Sales: [email_address]
    • MiraiBio Support Menu
      • Community
      • Knowledge Base
      • Blog
    • Webinars: http://www.MiraiBio.com/Webinars
    Resources