CENTENARIO DE LA CREACIÓN DE LOS ESTUDIOS MICROBIOLÓGICOS EN LA UNIVERSIDAD NACIONAL DE LA PLATA 1912 – 2012 V JORNADAS DE MICROBIOLOGIA CLINICA, INDUSTRIAL Y AMBIENTAL DE LA PROVINCIA DE BUENOS AIRES La Plata, Prov. de Buenos Aires 8, 9 y 10 de octubre de 2012 Carrera de Microbiología Clínica e Industrial Facultad Ciencias Veterinarias. UNLP.BIOFILMS EN DIFERENTES INDUSTRIAS. ALTERNATIVAS PARA SUCONTROLGuiamet PatriciaINIFTA, Departamento de Química, Facultad de Ciencias Exactas, UNLP, CCT La Plata - CONICET. CC16, Suc. 4 (1900), La Plata, Argentina.Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CONICET.firstname.lastname@example.org
Reuniones relevantes ( + de 70)■ First Workshop Argentine-USA (CONICET-NSF)on Biodeterioration of Materials, INIFTA, La Plata,1984.
■ Dahlem Conference on the Structure and Functions of Biofilms, Berlin, Germany, 1988.“Redox/metal dissolution in biofilms” H.A.Videla.
Medio Productos Ausencia de O2 corrosivos Zona anaeróbicaCátodo pH Cátodo Ánodo Metal
“Fungal contaminants of jet fuels”. (1995). H.A.Videla, P.S.Guiamet, E.H.Reinoso. Biodeterioration & Biodegradation 9 (A. Bousher, N. Chaudne, R. G. Edyvean, ed.), p. 615-620." Activity of isothiozolone biocide against Hormoconis resinae in pure and mixed biofilms". (1996). P.S.Guiamet and C.C.Gaylarde. World Journal Microbiology & Biotechnology, Vol.12, p. 395-397." Microbiological Spoilage of Aqueous Based Surface Coatings". (1996). P.S.Guiamet and H.A.Videla, Corrosion Reviews, Vol. 14, (1-2), p.47-57.
“Antifouling effects of two satured copper coating applied on carbonPanel de cobre - Panel testigostructures" (2008) de cobre ) steel (sin cubierta Guiamet P., Gómez de Saravia S. Revista Metalurgia 44 (5): 398-405 (ISSN 0034-8570)
Understanding biocorrosion in practical cases ofdifferent industrial systems Patricia Guiamet., Paola Lavin, Mónica Zapponi, Sandra Gómez de Saravia
The aims are a) to present several case studies of MIC found in three plants of extraction andsecondary oil recovery by water injection, due to operating problems. Plants are located in theprovinces of Neuquén (systems 1 and 2) and Mendoza (system 3) b) to suggest possiblesolutions for these problems.
System 1• SRB concentration up to 100 times higher than the maximum value of 10 4,generating then H2S.• The most important problem was connected with the use of a non oxidizingbiocide (acrolein). It was started with two patching per week of 1100 ppm, each ofthem during one hour.• At the end of 2004 a dispersant plus biocide was used. Pressure increased somuch that serious damages occurred in the system.• Some 15m3/hr. of fresh water from the Colorado river enter to the watertreatment system into the washing tank. This could be a source of nutrients for theSRB.
System 2• SRB (1015 mo/mL) were obtained. These high values observed in differentsystems are frequently due to staff dismissals caused by socioeconomic problemsor to operators working without the suitable training.•Tetrakishydroxymethyl phosphonium sulphate (THPS) was applied.There are high H2S concentrations of genetic and microbiological origin.•The treatment of water to be reinjected is not very effective. BRS PIAS•There are too much solids. 16 14 12 10 cal do s 8 6 4 2 0 Ene Feb Mar Abr May Jun Jul Ago Ent. Planta Sda. Planta Pozo Iny, o Colector
System 3 • Blockage of the wells by solids (> 40% per year). The well blockage causes asignificant injection pressure increase. • There is a generalized problem of corrosion in the valves. • The processes of the plant are not automatic . It is an operator who regulates thepumps and do other operations manually, so the smallest periods of concentrations isenough to cause problems. • There are high concentrations of sulphides would be deposited on the pipes. • Biocides used were glutaraldehyde, acrolein and others.
ALTERNATIVAS PARA SU CONTROL “An innovative method for preventing biocorrosion through microbial adhesion inhibition”. (1999). P.S.Guiamet, S.G.Gomez de Saravia and• Productos Naturales H.A.Videla. International Biodeterioration & Biodegradation,43 (1-2), 31-35. “ The antimicrobial activity of an aqueous extract of Brassica nigra “. Gómez de Saravia S. G.and Gaylarde C.C. International Biodeterioration and Biodegradation 41 (1998) 145-148Cichorium intybusCentaurea cyanus• Productos Químicos " Activity of isothiozolone biocide against Hormoconis resinae in pure and mixed biofilms". (1996). P.S.Guiamet and C.C.Gaylarde. World Journal Microbiology & Biotechnology, Vol.12, p. 395-397.
Global solution proposal for problems of the systems 1, 2 and 3∗ Service of microbiological management of the systems and a set ofconsultancies and specific services.∗ To collect, organize and display all the information related with microorganisms,as a tool for monitoring, controlling and preventing the bacteria presence in the oilsystems.∗ To train the operators and responsible staff of the plants in order to decrease atthe maximum operational mistakes∗ Theoretical and practical courses on biofouling, biofilms and biocorrosion issueswill be provided to the operators.∗ Collecting qualitative-quantitative data from planktonic and sessile bacteriaproducing biofilms of the systems.∗ Monitoring through sampling devices∗ To propose the use of the suitable biocides according to studies performed byauthorized staff.
Conclusiones Bacterias heterotróficas Potencial riesgo mesófilas aeróbicas de biocorrosión Bacterias productoras de ácido Bacterias sulfato reductoras Bacterias reductoras de sulfitoBajos valores de pH Riesgo de corrosión inorgánica Altas concentraciones de cloruros y sulfatos en el lixiviado
Plan de seguimiento a largo plazo… Estudios Estudiosfisicoquímicos microbiológicos EVITAR PARADAS DE PLANTA, RECAMBIO DE PIEZAS Y PÉRDIDAS ECONÓMICAS SIGNIFICATIVAS