MIqPCR - MIQE - RDML

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MIqPCR - MIQE - RDML

  1. 1. MIqPCR A Untergasser
  2. 2. Scope of the guidelines  Aims to describe quantitative PCR.  Technical guideline to create a meaningful RDML file correctly (overcome limits of XML).  Extended/replaced by MIQE in future.
  3. 3. Level of implementation  MIqPCR was published with RDML v1.0.  MIQE is in the focus of the journals.  qPCR-Ninja is developed to support RDML and MIQE.
  4. 4. Status, contributors, funding  MIqPCR is dead.  MIQE is under active development
  5. 5. MIQE Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT
  6. 6. Scope of the guidelines  MIQE - minimum information for publication of quantitative real-time PCR experiments  entire qPCR workflow  experimental design  sample information  sample processing  target information  oligos and probes  qPCR protocol  qPCR validation  data analysis
  7. 7. Level of implementation  creation of public awareness  presentations on numerous scientific meetings  company tours  part of qPCR courses  letter to editors  follow up papers  standard data format RDML (listed as MIqPCR @ MIBBI)  cited in 185 papers since 2009  guidelines adopted by journals  Clinical Chemistry  Nucleic Acids Research  BMC-Series journals
  8. 8. RDML  1.0 since 2008  1.1 to be released soon  2.0 planned  Taken up by different companies  Life Technologies  Bio-Rad  Biogazelle
  9. 9. Status & funding  Continued effort to create public awareness  Active discussion with industry on what is acceptable under the MIQE label  Planning for MIQE 2.0  Strengthen link with RDML  Enhance development of website  No dedicated MIQE funding

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