DNA Technology

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DNA Technology

  1. 1. APPLICATIONS OF DNA TECHNOLOGY One of the defining features of modern biology is the extensive use of the technology of gene manipulation . It is now possible to manipulate DNA directly to produce recombinant DNA . The manipulated genes can be replaced back into the original, or a different, organism to produce transgenic plants and animals.
  2. 2. Applications of DNA Technology <ul><li>1) The Human Genome Project: genetic mapping, DNA sequencing, genome analysis/comparison </li></ul><ul><li>2) Human Therapeutics: detecting genetic disorders, gene therapy </li></ul><ul><li>3) Forensic Uses: DNA profiling </li></ul><ul><li>4) Agriculture: transgenic plants, production of BST </li></ul>
  3. 3. The Human Genome Project <ul><li>The genome of an organism is it’s complete complement of genetic information </li></ul><ul><li>Completed in 2003, the international Human Genome Project was a 13-year project coordinated by the U.S. Department of Energy and the National Institutes of Health. </li></ul><ul><li>Project goals were to … </li></ul><ul><li>- identify all the approximately 20,000-25,000 genes in human DNA, </li></ul><ul><li>- determine the sequences of the 3 billion chemical base pairs that make up human DNA </li></ul><ul><li>- store this information in databases </li></ul><ul><li>- improve tools for data analysis </li></ul><ul><li>- address the ethical, legal, and social issues that may arise from the project. </li></ul>
  4. 4. <ul><li>The human genome project has been achieved using 3 approaches: </li></ul><ul><li>a) GENETIC MAPPING </li></ul><ul><li>b) PHYSICAL MAPPING </li></ul><ul><li>c) DNA SEQUENCING </li></ul><ul><li>Firstly however, the desired DNA sequences must be amplified. The process used to do this is the POLYMERASE CHAIN REACTION (PCR) </li></ul>

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