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The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3
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The wood frog as a model to study the pathogenesis and host-pathogen interactions of frog virus 3

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2013 International Symposium on Ranaviruses …

2013 International Symposium on Ranaviruses
by Maria Forzan

Published in: Education, Lifestyle, Technology
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  • Images:Snailtail.com – D hydeiSilkwormshop.com – D melanogsterAnimal Planet website (how stuff works) – Earth wormFlukefarms.com – meal wormClipart - cricket
  • 0.25ml(104.1 pfu)/adult
  • C03-10 dead ranavirus Apr 22, 2013
  • Spleens T7CTL – T6 – T7
  • Transcript

    • 1. Rana sylvatica (Lithobates sylvaticus) as a model to study FV3 María J. Forzán1,2, Kathleen M. Jones2 1Canadian Cooperative Wildlife Health Centre 2Atlantic Veterinary College 2nd Ranavirus Symposium Tennessee, July 2013
    • 2. Laboratory vs. field research • Laboratory vs. field research Source: Yale University Historical Library
    • 3. Species selection • Native species • Widespread • Sympatric • Suitable for captive breeding
    • 4. • Native species • Widespread • Sympatric • Suitable for captive breeding Species selection the wood frog
    • 5. Wood frog • Fam. Ranidae (Rana or Lithobates) • 9 species Canada • Terrestrial • Spread across NA • Broadly sympatric • Naturally host of ranavirus • Life span >3-5 y
    • 6. Wood frogs in the lab • Housing/feeding of pre- & post-metamorphic frogs • breeding in captivity? • Dosing of infective virus (FV3) • Collection and evaluation of blood samples • cellular and humoral responses • Collection of tissues • pathogenesis • PCR • virus isolation • histopathology • immunohistochemistry
    • 7. Eggs for tadpoles and metamorphs
    • 8. Eggs for tadpoles and metamorphs
    • 9. Eggs for tadpoles and metamorphs
    • 10. Froglets from tadpoles
    • 11. Froglets from tadpoles
    • 12. Froglets from tadpoles
    • 13. Housing tadpoles-metamorphs
    • 14. Adults
    • 15. Adults @AVC
    • 16. Housing • Temperature 17-23°C • Adults fed • CK, MW, EW • Froglets fed • Dm, Dh, CK, MW • Tadpoles fed • FF, Blet Extremely hard! • 75% adults (44/59, 6mo) • 72% froglets (81/112, 8mo) Date (mm-dd) Cage Frogs Food left Fed Comments Alive Dead C01 4x 1CK Ø 8MW Not hungry C02 C04 C05 C06 C07 C08 C09 C10 C03
    • 17. Oral dosing 107.7 pfu / ml
    • 18. Oral dosing 0.25ml / adult 0.05ml / 1-y-old
    • 19. LD50 1-year-old frogs (3.5 cm SV) • raised from tadpoles • oral infection – 0.05ml • euthanized when terminal (*) Dose (pfu/frog) 105.4 104.4 103.4 102.4 101.4 100.4 Control
    • 20. LD50 1-year-old frogs (3.5 cm SV) • raised from tadpoles • oral infection – 0.05ml • euthanized when terminal (*) Dose (pfu/frog) % (*) 105.4 100 104.4 100 103.4 80 102.4 20 101.4 0 100.4 0 Control 0 103.5 pfu/frog or 104.8 pfu/ml
    • 21. Pathogenesis • Adults (wild-caught) • 5.3 cm SV • Killed at 0.25, 0.5, 1, 2, 4, 9 and 14 days post- infection • Blood, tissues and abdominal fluid collected
    • 22. Blood samples
    • 23. Blood cells: N, Eo, B, Lym, Th, Ery
    • 24. End day
    • 25. Shedding – fecal swab (PCR)
    • 26. Immunity – Abs, IF-α/β
    • 27. Pathogenesis – PCR, H&E , IHC
    • 28. Pathogenesis – PCR, H&E , IHC
    • 29. Pathogenesis – PCR, H&E , IHC
    • 30. Spleens: Ctrl(-), 9 dpi, 14 dpi
    • 31. Done / In progress Housing and feeding of pre- and post-metamorphic frogs Dosing of infective virus (FV3) Collection and evaluation of blood samples • cellular and humoral responses • Pathogenesis • PCR, virus isolation, histopathology, immunohistochemistry
    • 32. Acknowledgments Unaccredited images from: Snailtail.com, Silkwormshop.com, Flukefarms.com, Animal Planet , CALA, Acadia University and Env. Canada websites
    • 33. Rana sylvatica (Lithobates sylvaticus) as a model to study FV3 • Native to North America • Fam. Ranidae (Rana or Lithobates) • Widespread (Canada, Eastern USA) • 9 species Canada • broadly sympatric • Suitable for captive breeding (possibly) • Naturally host of ranavirus • Life span >3-5 y
    • 34. Wood frogs in the laboratory • Housing/feeding of pre- and post-metamorphic frogs • recommendations • breeding in captivity (?) • Dose of infective virus (FV3) • LD50 • oral dosing • Blood samples • cellular & humoral responses • Tissues • pathogenesis • PCR, virus isolation • histopathology, immunohistochemistry

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