11 20-09 mbsb single molecule techniques.ppt

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11 20-09 mbsb single molecule techniques.ppt

  1. 1. • – – –• – – –
  2. 2. • – – – –
  3. 3. • – – –•••
  4. 4. •••
  5. 5. Image from: http://www.bnl.gov/rhic/news/102307/story3.asp
  6. 6. Images from: Hecht Optics 4th ed. (2002)
  7. 7. Image from: http://d-d-photographics.comImage from: http://www.aanda.org
  8. 8. •Images from: Hecht Optics 4th ed. (2002)
  9. 9. • • •• ••
  10. 10. Image from: http://www.olympusmicro.com/primer/techniques/fluorescence/anatomy/ix70fluorescence.html
  11. 11. ••
  12. 12. e- current out(photon)
  13. 13. Image from: Zhang et. al. J. Phys. B: At. Mol. Opt. Phys. 42 (2009)
  14. 14. •••
  15. 15. Excitation InImage from: Zheng-Xing et al. ACTA BIOCHIMICA et Fluorescence OutBIOPHYSICA SINICA. 2003, 35(4): 381-386
  16. 16. Excitation InPinhole Fluorescence Out
  17. 17. Image from: http://www.olympusfluoview.com/theory/confocalintro.html
  18. 18. Fluorescence Out
  19. 19. 102 nm10 um
  20. 20. •••
  21. 21. ••
  22. 22. V Image from: Smith et al Science. 243 4888 (1989)
  23. 23. Image from: Deutsch Science. 240 4854 (1988)
  24. 24. Image from: Bensimon et. al. Phys. Rev. Lett. 74 (1995)
  25. 25. Image from: Kim & Larson Nucleic Acids Research. (2007)
  26. 26. Image from: Kim & Larson Nucleic Acids Research. (2007)
  27. 27. Image from: Galletto et al. Nature. 443 (2006)
  28. 28. Image from: Galletto et al. Nature. 443 (2006)
  29. 29. Image from: Galletto et al. Nature. 443 (2006)
  30. 30. •••
  31. 31. Image from: Hein et al PNAS. 105 38 (2008)
  32. 32. Confocal STED In vivo stained ERImage from: Hein et al PNAS. 105 38 (2008)
  33. 33. Image from: Hein et al PNAS. 105 38 (2008)
  34. 34. Image from: Greenfield et al PLoS Biology. (2009)
  35. 35. •••
  36. 36. • QE of > 0.1 • > 20,000 cm-1 M-1Table from: Gell, Brockwell & Smith Handbook of Single Molecule Spectroscopy. (2006)
  37. 37. •••
  38. 38. Image from: Abe et al Biochemistry. 42:51 (2003)
  39. 39. Image from: English et al Nat. Chem. Biol. 2:2 (2006)
  40. 40. Image from: English et al Nat. Chem. Biol. 2:2 (2006)
  41. 41. Image from: Sase et al PNAS. 94 (1997)
  42. 42. Image from: Sase et al PNAS. 94 (1997)
  43. 43. Image from: Yildiz et al Science. 27 (2003)
  44. 44. Image from: Yildiz et al Science. 27 (2003)
  45. 45. Image from: Yildiz et al Science. 27 (2003)
  46. 46. Image from: Liu et al Science. 322 (2008)
  47. 47. Image from: Liu et al Science. 322 (2008)
  48. 48. Image adapted from: Shirude & Balasubramanian Biochemie. 90:8 (2008)
  49. 49. Image from: Shirude & Balasubramanian Biochemie. 90:8 (2008)
  50. 50. Image from: PDB entry 3CRX Image from: Ha Biochemistry. 43 (2004)
  51. 51. Image from: Ha Biochemistry. 43 (2004)
  52. 52. 2nm 4nmImage from: http://www.aist.go.jp/
  53. 53. • – –• – –
  54. 54. Image from: Chen et al Biophys J. 92 (2007)
  55. 55. Image from: Capitanio et al. J. Opt. A: Pure Appl. Opt. 9 (2007)
  56. 56. Image from: Sönnichsen Plasmons in metal nanostructures. (2001)
  57. 57. • – – – –• – – –
  58. 58. Image from: Sönnichsen et. al. Nature Biotechnology. 23 (2005)
  59. 59. Image from: Reinhard et. al. PNAS. 104:8 (2006)
  60. 60. Image from: Jun et. al. PNAS. 106:42 (2009)
  61. 61. Image from: Jun et. al. PNAS. 106:42 (2009)

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