11 20-09 mbsb single molecule techniques.ppt
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11 20-09 mbsb single molecule techniques.ppt Presentation Transcript

  • 1. • – – –• – – –
  • 2. • – – – –
  • 3. • – – –•••
  • 4. •••
  • 5. Image from: http://www.bnl.gov/rhic/news/102307/story3.asp
  • 6. Images from: Hecht Optics 4th ed. (2002)
  • 7. Image from: http://d-d-photographics.comImage from: http://www.aanda.org
  • 8. •Images from: Hecht Optics 4th ed. (2002)
  • 9. • • •• ••
  • 10. Image from: http://www.olympusmicro.com/primer/techniques/fluorescence/anatomy/ix70fluorescence.html
  • 11. ••
  • 12. e- current out(photon)
  • 13. Image from: Zhang et. al. J. Phys. B: At. Mol. Opt. Phys. 42 (2009)
  • 14. •••
  • 15. Excitation InImage from: Zheng-Xing et al. ACTA BIOCHIMICA et Fluorescence OutBIOPHYSICA SINICA. 2003, 35(4): 381-386
  • 16. Excitation InPinhole Fluorescence Out
  • 17. Image from: http://www.olympusfluoview.com/theory/confocalintro.html
  • 18. Fluorescence Out
  • 19. 102 nm10 um
  • 20. •••
  • 21. ••
  • 22. V Image from: Smith et al Science. 243 4888 (1989)
  • 23. Image from: Deutsch Science. 240 4854 (1988)
  • 24. Image from: Bensimon et. al. Phys. Rev. Lett. 74 (1995)
  • 25. Image from: Kim & Larson Nucleic Acids Research. (2007)
  • 26. Image from: Kim & Larson Nucleic Acids Research. (2007)
  • 27. Image from: Galletto et al. Nature. 443 (2006)
  • 28. Image from: Galletto et al. Nature. 443 (2006)
  • 29. Image from: Galletto et al. Nature. 443 (2006)
  • 30. •••
  • 31. Image from: Hein et al PNAS. 105 38 (2008)
  • 32. Confocal STED In vivo stained ERImage from: Hein et al PNAS. 105 38 (2008)
  • 33. Image from: Hein et al PNAS. 105 38 (2008)
  • 34. Image from: Greenfield et al PLoS Biology. (2009)
  • 35. •••
  • 36. • QE of > 0.1 • > 20,000 cm-1 M-1Table from: Gell, Brockwell & Smith Handbook of Single Molecule Spectroscopy. (2006)
  • 37. •••
  • 38. Image from: Abe et al Biochemistry. 42:51 (2003)
  • 39. Image from: English et al Nat. Chem. Biol. 2:2 (2006)
  • 40. Image from: English et al Nat. Chem. Biol. 2:2 (2006)
  • 41. Image from: Sase et al PNAS. 94 (1997)
  • 42. Image from: Sase et al PNAS. 94 (1997)
  • 43. Image from: Yildiz et al Science. 27 (2003)
  • 44. Image from: Yildiz et al Science. 27 (2003)
  • 45. Image from: Yildiz et al Science. 27 (2003)
  • 46. Image from: Liu et al Science. 322 (2008)
  • 47. Image from: Liu et al Science. 322 (2008)
  • 48. Image adapted from: Shirude & Balasubramanian Biochemie. 90:8 (2008)
  • 49. Image from: Shirude & Balasubramanian Biochemie. 90:8 (2008)
  • 50. Image from: PDB entry 3CRX Image from: Ha Biochemistry. 43 (2004)
  • 51. Image from: Ha Biochemistry. 43 (2004)
  • 52. 2nm 4nmImage from: http://www.aist.go.jp/
  • 53. • – –• – –
  • 54. Image from: Chen et al Biophys J. 92 (2007)
  • 55. Image from: Capitanio et al. J. Opt. A: Pure Appl. Opt. 9 (2007)
  • 56. Image from: Sönnichsen Plasmons in metal nanostructures. (2001)
  • 57. • – – – –• – – –
  • 58. Image from: Sönnichsen et. al. Nature Biotechnology. 23 (2005)
  • 59. Image from: Reinhard et. al. PNAS. 104:8 (2006)
  • 60. Image from: Jun et. al. PNAS. 106:42 (2009)
  • 61. Image from: Jun et. al. PNAS. 106:42 (2009)