11 20-09 mbsb single molecule techniques.ppt
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11 20-09 mbsb single molecule techniques.ppt

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    11 20-09 mbsb single molecule techniques.ppt 11 20-09 mbsb single molecule techniques.ppt Presentation Transcript

    • • – – –• – – –
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    • •••
    • Image from: http://www.bnl.gov/rhic/news/102307/story3.asp
    • Images from: Hecht Optics 4th ed. (2002)
    • Image from: http://d-d-photographics.comImage from: http://www.aanda.org
    • •Images from: Hecht Optics 4th ed. (2002)
    • • • •• ••
    • Image from: http://www.olympusmicro.com/primer/techniques/fluorescence/anatomy/ix70fluorescence.html
    • ••
    • e- current out(photon)
    • Image from: Zhang et. al. J. Phys. B: At. Mol. Opt. Phys. 42 (2009)
    • •••
    • Excitation InImage from: Zheng-Xing et al. ACTA BIOCHIMICA et Fluorescence OutBIOPHYSICA SINICA. 2003, 35(4): 381-386
    • Excitation InPinhole Fluorescence Out
    • Image from: http://www.olympusfluoview.com/theory/confocalintro.html
    • Fluorescence Out
    • 102 nm10 um
    • •••
    • ••
    • V Image from: Smith et al Science. 243 4888 (1989)
    • Image from: Deutsch Science. 240 4854 (1988)
    • Image from: Bensimon et. al. Phys. Rev. Lett. 74 (1995)
    • Image from: Kim & Larson Nucleic Acids Research. (2007)
    • Image from: Kim & Larson Nucleic Acids Research. (2007)
    • Image from: Galletto et al. Nature. 443 (2006)
    • Image from: Galletto et al. Nature. 443 (2006)
    • Image from: Galletto et al. Nature. 443 (2006)
    • •••
    • Image from: Hein et al PNAS. 105 38 (2008)
    • Confocal STED In vivo stained ERImage from: Hein et al PNAS. 105 38 (2008)
    • Image from: Hein et al PNAS. 105 38 (2008)
    • Image from: Greenfield et al PLoS Biology. (2009)
    • •••
    • • QE of > 0.1 • > 20,000 cm-1 M-1Table from: Gell, Brockwell & Smith Handbook of Single Molecule Spectroscopy. (2006)
    • •••
    • Image from: Abe et al Biochemistry. 42:51 (2003)
    • Image from: English et al Nat. Chem. Biol. 2:2 (2006)
    • Image from: English et al Nat. Chem. Biol. 2:2 (2006)
    • Image from: Sase et al PNAS. 94 (1997)
    • Image from: Sase et al PNAS. 94 (1997)
    • Image from: Yildiz et al Science. 27 (2003)
    • Image from: Yildiz et al Science. 27 (2003)
    • Image from: Yildiz et al Science. 27 (2003)
    • Image from: Liu et al Science. 322 (2008)
    • Image from: Liu et al Science. 322 (2008)
    • Image adapted from: Shirude & Balasubramanian Biochemie. 90:8 (2008)
    • Image from: Shirude & Balasubramanian Biochemie. 90:8 (2008)
    • Image from: PDB entry 3CRX Image from: Ha Biochemistry. 43 (2004)
    • Image from: Ha Biochemistry. 43 (2004)
    • 2nm 4nmImage from: http://www.aist.go.jp/
    • • – –• – –
    • Image from: Chen et al Biophys J. 92 (2007)
    • Image from: Capitanio et al. J. Opt. A: Pure Appl. Opt. 9 (2007)
    • Image from: Sönnichsen Plasmons in metal nanostructures. (2001)
    • • – – – –• – – –
    • Image from: Sönnichsen et. al. Nature Biotechnology. 23 (2005)
    • Image from: Reinhard et. al. PNAS. 104:8 (2006)
    • Image from: Jun et. al. PNAS. 106:42 (2009)
    • Image from: Jun et. al. PNAS. 106:42 (2009)