Environmental applications brochure

Environmental Applications

There’s no question that there’s a heightened focus on the environment— and the impact that modern industry and our own lifestyles have on it. The wealth of research directly linking contaminants in our water, air and soil to illness and disease has stepped up efforts, particularly in North America, to reduce the amount of waste and chemical by- products we add to our surroundings. In the United States, the Environmental Protection Agency (EPA) is the governing body that sets limits on the amount of chemical by-products that may be found in the water that we drink and the soil in which we grow our food and sustain our livestock. For many years Metrohm has worked closely with the EPA on methods that determine these limits—and our instruments are highly valued for their precision and adaptability as applications are refined and improved. You won’t find a better brand instrument for your analysis than Metrohm. All manufacturing and assembly takes place in our own production facilities in Switzerland by our very own Metrohm employees. They don’t cut corners, and we never use inferior parts or raw materials. That’s why our systems come with the best warranties in the industry – we stand behind our products longer than other brands because we know we’ve built them to last! So take a minute to discuss your applications and any special requirements with us, you’ll be glad you did!2

Quick Reference:EPA Method Description Metrohm Systems Page No.120.1 Conductance (Specific Conductance, µmhos at 25°C) 856 Conductivity Module 18-19130.2 Total Hardness (mg/L as CaCO3) (Titrimetric, EDTA) 855 Robotic TitroSampler 18-19 pH Analysis of Drinking, Surface and Saline Waters,150.1 Domestic and Industrial Wastes and Acid Rain 855 Robotic TitroSampler 18-19 (Atmospheric Deposition) Determination of Dissolved Hexavalent Chromium in 850 Professional IC,218.6 Drinking Water, Groundwater and Industrial Wastewater 12-13 881 Compact IC pro Effluents by Ion Chromatography 850 Professional IC, Determination of Inorganic Anions by Ion300.0/9056 881 Compact IC pro, 18-19 Chromatography 882 Compact IC plus Determination of Inorganic Anions in Drinking Water by 850 Professional IC,300.1 4-5 Ion Chromatography 881 Compact IC pro310.1 Alkalinity (Titrimetric, pH 4.5) 855 Robotic TitroSampler 18-19 Determination of Perchlorate in Drinking Water by Ion 850 Professional IC,314.0 8-9 Chromatography 881 Compact IC pro Determination of Inorganic Oxyhalide Disinfection By- products in Drinking Water. Using Ion Chromatography 850 Professional IC,317.0 6-7 with the Addition of a Post-column Reagent for Trace 881 Compact IC pro Bromate Analysis Determination of Inorganic Oxyhalide Disinfection By- products in Drinking Water Using Ion Chromatography 850 Professional IC,326.0 6-7 with the Addition of a Suppressor-acidified Post-column 881 Compact IC pro Reagent for Trace Bromate Analysis330.1 Chlorine, Total Residual (Titrimetric, Amperometric) 855 Robotic TitroSampler 18-19 Determination of Perchlorate in Drinking Water by Ion Chromatography with Suppressed Conductivity and 850 Professional IC,332.0 10-11 Electrospray Ionization Mass Spectrometry (IC/ESI/MS or 881 Compact IC pro IC/ESI/MS/MS)340.2 Fluoride (Potentiometric, Ion Selective Electrode) 855 Robotic TitroSampler 18-19 Nitrogen, Ammonia (Potentiometric, Ion Selective350.3 855 Robotic TitroSampler 18-19 Electrode) Perchlorate in Water, Soils and Solid Wastes Using 850 Professional IC,SW 6860 Ion Chromatography/Electrospray Ionization/Mass 10-11 881 Compact IC pro Spectrometry (IC/ESI/MS or IC/ESI/MS/MS) Determination of Hexavalent Chromium in DrinkingSW 7199/draft 850 Professional IC, Water, Groundwater and Industrial Wastewater Effluents 12-13SW 7194 881 Compact IC pro by Ion ChromatographyASTM Method Description Metrohm Systems Determination of Dissolved Alkali and Alkaline Earth 850 Professional IC,D6919-09 Cations and Ammonium in Water and Wastewater by 881 Compact IC pro, 14-15 Ion Chromatography 882 Compact IC plusAdditional Description Metrohm SystemsApplications 850 Professional IC,EPA 6800 Trace Metal Speciation by IC-ICP/MS – Hg, As & Cr 881 Compact IC pro, 16-17 882 Compact IC plus Toll-free 866-METROHM (638-7646) 3

METHOD Anions by EPA 300.0 (A&B) and 300.1 (A&B) For this popular method, our budget-friendly Compact IC Our patented Dosino® top-down dosing technology, with 858 Sample Processor system automates your routine eliminates the formation of air bubbles—a common side- sample prep tasks as well as the final analysis. effect of traditional dosing that negatively affects results. It also speeds up your run because one sample is being It can: prepare the eluent from concentrates; filter the diluted at the same time another is being analyzed. samples; prepare the multipoint calibration curve and dilute those samples that are over the calibration. Automatic multi-point calibration from a single high standard saves countless hours of manual prep work, as For added convenience, this system is also available with an the system will precisely measure the right amount of inline eluent generator. standard and perform all the tasks up to and including the calculations. System highlights A state-of-the-art ultrafiltration cell works on the Powerful, yet easy-to-use MagIC Net software. It simplifies cross-sectional flow principle to give you long and your daily data management; it quickly and easily verifies uncompromised operational life (see picture below); that your samples fall within the calibration range and your depending on your matrix, the filter can last anywhere from continuous calibration check samples are in compliance 100 to 300 samples before it needs changing—goodbye with the method. Just highlight the samples of interest, and expensive syringe filters and filter caps! And because it is with the click of one button it graphs the results for quick automatically rinsed with a combination of DI water and visual verification. Stop spending hours looking through sample after each injection, there is no threat of carry-over individual data files—this quick-click feature does it for you! contamination to worry about. These systems will also save time and reduce the amount of manual work associated with the method. For example, tried-and-true automated intelligent dilution saves you from having to figure out the correct calculations, only to then manually dilute your samples and rerun them. More on ultrafiltration pg. 204

METHOD Column: A Supp 5-150Standard Anions by 300.0 Eluent: 3.2mM Na2CO3/1.0mM NaHCO3 cond Flow Rate: 0.7mL/minμS/cm 2 Temperature: 25°C Injection Volume: 20µL 24 Detection: Suppressed conductivity 1 5 7 22 4 3 Peak RT Component Concentration 20 1 3.4 min Fluoride 2 mg/L 2 5.4 min Chloride 5 mg/L 6 18 3 6.6 min Nitrite 5 mg/L 4 8.5 min Bromide 10 mg/L 16 5 9.9 min Nitrate 10 mg/L 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 min 6 12.5 min Phosphate 10 mg/L 7 14.7 min Sulfate 10 mg/L Column: A Supp 7-250 Eluent: 3.6mM Na2CO3 Flow Rate: 0.7mL/min Temperature: 45°COxyhalides & Standard Anions by 300.1 (A&B) Injection Volume: 20µL cond Detection: Suppressed conductivityμS/cm 4 28 Peak RT Component Concentration 1 5.0 min Fluoride 2 mg/L 26 10 2 7.8 min Chlorite 10 mg/L 12 24 3 3 8.2 min Bromate 20 mg/L 6 8 1 4 9.3 min Chloride 3 mg/L 22 2 5 10.2 min System Peak - 7 20 11 6 12.0 min Nitrite 10 mg/L 18 7 15.6 min Bromide 20 mg/L 9 5 8 16.6 min Chlorate 20 mg/L 16 9 18.0 min DCA 5 mg/L 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 min 10 19.1 min Nitrate 10 mg/L 11 21.7 min Phosphate 20 mg/L 12 25.0 min Sulfate 15 mg/L Application work: AW CH6-0968-112008 Determination of anions & oxyhalides Toll-free 866-METROHM (638-7646) 5

METHOD Bromate by EPA 317.0 and 326 The use of ozone as a disinfectant is gaining in popularity, System highlights even though its by-product bromate has been identified For stand-alone bromate detection our Compact 881 IC as a potential carcinogen—creating the need for its and new 887 UV/VIS detector is an excellent and cost- monitoring at trace levels. The EPA has set its daily exposure effective system—perfect for the budget-conscious lab only (through household products and drinking water) maximum concerned with this single analysis, or any lab with a high contamination limit (MCL) at 10ppb; with an MCL goal of enough sample volume to dedicate an instrument for this 0ppb. analysis. 62.50 Column: A Supp 10-250 Concentration 6 Eluent: 20mM NaHCO3 Flow Rate: 1mL/min Temperature: 45°C Injection Volume: 1mL 5 Detection: UV/VIS 4 3 12 Area 1 2 3 4 5 6 7 8 9 10 11 12 + 02 Bromate Calibration Method ASupp10_250Malvoz.mtw Equation Q = 43.663·A RSD 0.472 % (mAu) Correlation Coefficient 0.999997 Standards Bromate (ug/L) 3.5 Chlorite Levels 1, 2, 5, 10, 30, 50 3.0 2.5 Bromate Nitrite 2.0 Iodate 1.5 1.0 0.5 Peak RT Component Concentration 0.0 1 5.0 min Iodate 1 µg/L -0.5 2 6.6 min Chlorite 1 µg/L -1.0 3 9.4 min Bromate 1 µg/L 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 min 4 13.8 min Nitrite 1 µg/L Application work: AW BE6-0028-052009b Determination of iodate, chlorite, bromate and nitrite in drinking and waste water6

For the majority of labs that test for more than just bromate limit.and/or need high sensitivity for trace-level analysis, there’sno system out there that can match the sub-ppb levels thatour 850 Professional IC and 887 UV/VIS Detector can reach.This system setup can be used in series with a conductivitydetector to detect low- to sub-ppb levels of commonanions and other oxyhalides along with bromate.Our IC systems feature post-column reagent (PCR) additionusing a high-pressure pump or Dosino top-down dosingsystem, which eliminates the need for gas tanks andpressurized bottles. Using the pump or Dosino, you canelectronically control the flow rate and consistently maintainthe ratio of PCR to mobile phase, which is critical for thecolor chemistry to properly work. This also significantlyreduces noise levels, paving the way for a better detection Toll-free 866-METROHM (638-7646) 7

METHOD Perchlorate by EPA 314.0 Perchlorate, a common contaminant of ground and Two systems allow you to easily perform this method— drinking water, has been identified as a culprit of thyroid choose the one that best suits your needs. Both easily dysfunction and a contributor to other health issues. So it’s detect, calibrate and report results down to 0.5 ppb levels— no wonder that many regulatory agencies, including the even in the presence of 1,000 ppm levels of chloride, EPA, have set limits on its concentration. carbonate and sulfate. No need to use an SPE cartridge to remove interfering ions from total dissolved solids. Typically a Metrohm A Supp 7 or Dual 4 column can be used for the analysis. (μS/cm) x min Column: A Supp 7-250 0.28 Eluent: 10mM Na2CO3/45% Acetonitrile 0.26 0.24 Flow Rate: 0.7mL/Min 0.22 Temperature: 45°C 0.20 0.18 Injection Volume: 500µL 0.16 Detection: Suppressed conductivity 0.14 0.12 0.10 0.08 0.06 Perchlorate Calibration 0.04 0.02 Method Perchlorate USEPA 314.0.imet 0.00 0.0 4.0 8.0 12.0 16.0 20.0 24.0 28.0 ppb RSD 1.382 % Correlation coefficient: 0.999932 Standards Perchlorate (ClO4 )-1 ug/L 0.50, 1.00, 3.02, 5.02, 10.04, Levels 20.15, 26.75 ug/L μS/cm 0.548 0.544 0.540 Perchlorate 0.533 0.536 0.532 Perchlorate by 314.0 0.528 0.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 min DI water spiked with 1000ppm each of chloride, carbonate and sulfate & Perchlorate at 0.5ppb Application work: AW US6-0130-022008 Determination of trace level perchlorate8

METHODSystem highlightsOur 850 Professional IC with 872 Extension Moduleperforms both regular anions and perchlorate in a singleinjection; and if you are looking to perform a two-dimensional IC (EPA 302), this is your ideal set-up.For a simple, dedicated system, you can’t go wrong withthe 881 Compact IC and 858 Autosampler. It’s as preciseand accurate as its professional counterpart, just with lessbells & whistles to match your less complex analysis. Toll-free 866-METROHM (638-7646) 9

METHOD Perchlorate by EPA 332/SW 6860 When confirmation is required for perchlorate, EPA System highlights Methods 332 and SW 6860 are the methods of choice. Both our Compact and Professional IC systems can easily The separation takes place inside the IC using an A Supp attach to a single quadrupole (MS) or triple quadrupole (MS/ 7 or Dual 4 column followed by detection using a mass MS) with electro spray ionizer (ESI). Our 850 Professional, spectrometer. Solvent is added directly into the mobile as well as our 881 and 882 Compact models can directly phase, which enhances the MS ionizer’s function and connect to Agilent® MS; running MagIC Net MS software increases column life by minimizing organics adhering to in the background allows Agilent’s Chemstation® software the column. The solvent-compatible MSM Suppressor is to completely control the system. designed to handle harsh solvents, which lowers overall operating costs as well. PERCHLORATE - 8 Levels Used. 8 Points. 8 Points Used. 33 QCs y = 3.0079*x - 0.0016 Relative Responses 6.75 R^2 = 0.99826813 6.5 6.25 6 5.75 Perchlorate Calibration 5.5 5.25 5 10ppt – 10ppb 4.75 4.5 R2 > 0.998 4.25 Column: A Supp 7-250 4 3.75 Eluent: 10mM Na2CO3/45% Acetonitrile 3.5 3.25 Flow Rate: 0.7mL/Min 3 2.75 Temperature: 45°C 2.5 2.25 Injection Volume: 100µL 2 Detection: Mass Spectrophotometer 1.75 1.5 1.25 1 0.75 0.5 0.25 0 -0.25 -0.5 -0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 Relative Concentration10

x102 7.8 7.6 7.4 0.5 ppb LCMRL 7.2 7 7 injections 6.8 6.6 Peak area % RSD = 1.4 6.4 6.2 6 5.8 5.6 5.4 5.2 5 4.8 4.6 4.4 4.2 4 3.8 3.6 3.4 3.2 3 2.8 2.6 2.4 2.2 2 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 0 21 21.5 22 22.5 23 23.5 24 24.5 25 Counts vs. Acquisition Time (min) MS Overlay Chromatogram Toll-free 866-METROHM (638-7646) 11

METHOD Hexavalent Chromium by EPA 218.6/SW 7199/Draft 7194 The measurement of hexavalent chromium in drinking System highlights water and wastewater is governed by EPA Method 218.6, As with bromate detection, our IC systems feature post- and in soils by SW 846 Method 7199. Both methods use column reagent addition using a high pressure pump or anion exchange chromatography followed by diphenyl Dosino top-down dosing system, which eliminates the carbazide (DPC) post-column chemistry. This produces a need for gas tanks and pressurized bottles. Using the pump colored complex of hexavalent chromium, which can then or Dosino, you can electronically control the flow rate and be detected by a UV/VIS detector. consistently maintain the ratio of PCR to mobile phase, which is critical for the color chemistry to properly work. This also significantly reduces noise levels, paving the way for a better detection limit. Cr+6_Dosino mAU Cr+6 3.2 Column: A Supp 5-150 Eluent: 12.8mM Na2CO3/4.0mM NaHCO3 Flow Rate: 0.8mL/Min 2.4 Temperature: 45°C Injection: 1275µL 1.6 Detection: UV/VIS 0.8 0.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 min 1ppb Cr+6 Cr VI Calibration Method Cr+6_218.6_Dosino_ASUPP5-150 RSD 0.8476% Cr+6 (Cr+6_Dosino) Correlation coefficient: 0.999989 (mAU) x min Standards Cr 6+ ng/L Levels 12.5,125,250,500,1000 & 5000ng/L 4.0 3.0 2.0 1.0 0.0 0.0 1000.0 2000.0 3000.0 4000.0 5000.0 ppt12

METHODVarious configurations of both the 850 Professional and881 Compact IC with 887 UV/VIS detector are availablefor stand-alone analysis—or analyses in series/parallel witha conductivity detector for additional anion and cationdetection.Flexible 850 cation high-pressure gradient systems allowyou to add an anion channel in the future without havingto purchase another instrument, software or autosampler.If you don’t foresee the need for such flexibility, thenconsider our 881 Compact IC with 887 UV/VIS detector.This system still gives you the ability to run hexavalentchromium with the same accuracy and precision, at a price/performance ratio more aligned to your requirements. Toll-free 866-METROHM (638-7646) 13

METHODCations by ASTM D6919-09Our Professional and Compact series cation IC systems can If you are planning to run only cations, or you’re willingsimultaneously determine the inorganic alkali and alkaline to switch runs between anions and cations in an effortearth cations—lithium, sodium, potassium, magnesium to save money on your IC purchase, then consider theand calcium—as well as ammonium cations in reagent more basic Compact 881 and 882 systems. They offer thewater, drinking water and wastewaters in a non-suppressed same precision and accuracy, just with less bells & whistlesmode. (and, it’s important to note, less flexibility—should your requirements change down the road).System highlightsSeveral 850 Professional models are available to run cations C4 or C5 columns can be used for the determination: inalone or anions and cations simultaneously, in series or in both cases, sodium and ammonia are baseline separated;parallel, with one autosampler. the C5 column is preferred in analyzing ammonia in presence of high levels of sodium. 6 Column: Metrosep C4-150 Eluent: 1.7mM HNO3/0.7mM DPA Flow Rate: 0.9mL/Min NH4 5 Temperature: 25°C Injection Volume: 20µL Detection: Conductivity 4 signal (µS/cm) Na Mg 3 K Li Ca 2 1 0 4 8 12 16 20 time (min) Toll-free 866-METROHM (638-7646) 15

METHOD Hg, As & Cr speciation by EPA 6800 Add a Metrohm IC to your ICP-MS for speciation System highlights studies! Inter-species transformations of chromium (or For speciation of metals and organometallics such as other species) require the use of specific isotope dilution mercury, selenium, arsenic and chromium, a hyphenated mass spectrometry (SIDMS). EPA Method 6800 is applied technique combines ion chromatography and inductively to evaluate and efficiently compensate for potential coupled plasma with a mass spectrometer (IC-ICP/MS). transformations during the sample preparation and measurement. It is especially useful for the determination of chromium (or other species) when species inter-conversion caused by sample storage, handling, or preparation is suspected. 200 Intensity [1000-counts] 150 blank monomethylarsenate dimethylarsenate arsenite arsenate 100 arsenobetaine 50 0 5 10 15 20 Time [min] 3000 Hg2+ 2500 199 Hg 200 Hg Intensity [counts] 2000 202 Hg 1500 CH3Hg+ 1000 500 0 1 2 3 4 5 Time [min]16

Our IC’s metal-free flow path is required because theseparation often uses highly buffered mobile phase; thesesamples have a high ionic strength that attacks the stainlesssteel of traditional HPLC components which, over time,leads to high maintenance costs. The PEEK flow path of ourIC system is inert to these matrices—there’s no corrosion ormetal leaching from the components, which helps minimizecontamination issues associated with low ppb and sub-ppbmeasurements.Our 850 Professional and 881/882 Compact IC systemscan directly connect to an Agilent ICP/MS; MagIC NetMS software runs in the background to enable completecontrol of the system and analyses by Agilent’s Chemstationsoftware. Toll-free 866-METROHM (638-7646) 17

AUTOMATED SYSTEMS Automated Environmental Analyzers It’s no surprise that with EPA methods, especially, precision testing with accurate, reproducible results is key. And it’s pretty much the norm now, that we’re all tasked to do more with less – less staff, less resources, less budget, less time. Metrohm automated systems are designed to address these everyday challenges you face in your workday. They’ll handle all the manual work you no longer have time — or staff — to do, and they’ll do it at a level of precision that beats the best, most seasoned taskmaster can do manually. And faster, too. Available in 9 models/configurations to handle any sample prep task and analyze drinking water and wastewater, these systems can automate up to 100 samples at a time and automatically pipette volumes from 10 mL to 50 mL; our Chlorine-specific system even handles volumes up to 100 mL. To avoid any interference at the titration step, conductivity measurements take place in the beaker prior to sampling. Additional features include: • A space-saving, built-in electrode input with temperature compensation and DI water pump • External pump for vessel aspiration • Various sample rack options to meet your specific application requirements • Easy-to-use tiamo ® software allows you to store and recall data at the touch of a button, as well as print, export and back up data to LIMS and other network systems18

AUTOMATED SYSTEMS DET pH 46.1 - DET pH 9 FP1 EP1 8 7 6pH 5 FP2 EP2 4 3 2 0 2 4 6 7 10 12 14 V [mL]Preprogrammed EPA methods include: pH pH pH pHSystem/Application(s) Alkalinity Alkalinity Alkalinity Chlorine Alkalinity Flouride Hardness Ammonia 150.1 150.1 150.1 150.1EPA Methods 310.1 310.1 310.1 330.1 310.1 340.2 130.2 350.3Conductivity optional via Yes Yes Yes Yes NoEPA 120.1ISE standard addition N/A Yes N/A Yes N/AavailableSample size 10 ml–50 ml 10 ml–50 ml 10 ml–50 ml 10 ml–50 ml 100 mlSampling technique Transfer pipette Transfer pipette Transfer pipette Transfer pipette Overflow pipetteTitration in external Yes Yes Yes Yes Yesvessel Toll-free 866-METROHM (638-7646) 19

AUTOMATED SYSTEMS TitrIC systems combine titration & IC This unique system only from Metrohm, automates the analysis of alkalinity, pH, conductivity, anions and cations. 855 Robotic Titrosampler Available in several configurations to best match your lab’s 28...100 samples, 75...200 mL applications and throughput, they all pair high-precision Titrando titrator and sample processor or titrosampler with Ultrafiltration Conductivity an 850 proffesional IC system. Anions Temperature F-, Cl-, NO2-, Br-, Simply place the samples—up to 100 of them—on the rack NO3-, HPO42-, SO42- pH value (the titrator also acts as the autosampler for the IC system), Cations Titration KA 8.2 (p value) and the TitrIC software takes over: it quickly determines all Li+, Na+, NH4+, ionic components with exceptional precision, and provides K+, Mg2+, Ca2+ Titration KA 4.3 (m value) completely reliable results. TitrIC Database, report Pre-configured systems are available from the most basic TitrIC-4 (temperature, alkalinity, pH, conductivity, anions) to the most advanced TitrIC-7 (additional features include cations and inline ultrafiltration).20

AUTOMATED SYSTEMSOnline and Atline Process AnalyzersAs a nation that continues to push the envelope of industry, Our systems are fully customizableit is important that all chemical production processes— With more than 30 years of expertise and experience inregardless of the type of industry they fall in—be monitored analytical process monitoring, we customize our systems tofor efficiency and quality. Our Applikon online and at-line perfectly match any process or application — from simple tosystems are fully integrated right at the process line for the complex. Our systems are all designed to keep productioncontinuous, precise monitoring of contaminants, precious efficient, cost-effective and, above all, in compliance withmetals and other ions/compounds. local, regional and governmental regulations.Perfectly suited to these environmental applications:• Hexachrome removal during drinking water production. This by-product has been identified as a carcinogen – and a known contaminant in the drinking water of many municipalities. Our systems are used to help water treatment plants monitor their production of safe drinking water.• Ammonia monitoring in sewage water treatment plants. Because high amounts of ammonia in spent water negatively impacts the quality of our natural water supply, our online analyzers measure around-the-clock to guarantee wastewater conformity with all local and legal requirements.• Cyanide and heavy metals in mining process waters. Water is vital to extracting precious metals from all kinds of ores—one of them being cyanide. Our systems excel at monitoring the levels of this highly toxic compound, as well as many others, in the spent process water. Toll-free 866-METROHM (638-7646) 21

FEATURE SPOTLIGHT An IC suppressor that lasts 10 years—really Other brands may follow the razor/blade marketing The difference is in the details: strategy becasue there’s big money to be made in We leave membrane technology behind—there’s no repeat consumables business. But that’s certainly not the membrane to clog, no long start-up nor will you see Metrohm philosophy. We believe that by giving you the deteriorated/diminished peaks or inconsistent results over best, most cost-effective system possible, you will become time. It also offers significantly lower noise, which translates our customer—and remain loyal, no tricks or gimmicks to better sensitivity. And 100% solvent compatibility opens necessary. up enormous possibilities for eluent modification. Our solvent-compatible suppressor is guaranteed to last 10 years—with no change in performance/results—or the replacement suppressor is free. Metrohm’s suppressor technology provides the best return on investment Let’s do the math: Metrohm IC sample Plug in your numbers 1 No. of samples analyzed per year 1,000 2 Suppressor replacement cost (ea.) $650* $ 3 No. of suppressors you replace per year 0 4 Yearly suppressor cost (multiply lines 2 & 3) $0.00 $ 5 If your throughput remains the same for 10 years x10 x10 6 10-year replacement cost (multiply lines 4 & 5) $0 $ *Price listed in USD as of 6/30/201122

FEATURE SPOTLIGHTUltrafiltration explainedSave your column, and save your budget A two-channel peristaltic pump pushes sample into theOur ultrafiltration cell traps unwanted particles suspended cell and pulls what’s needed through the filter, dischargingin your sample that would otherwise clog the column and what’s not. This intelligent push-pull process keeps the filteraffect your results. Think of it as a sandwich: two disc layers from clogging with unnecessary particle buildup, extendingseparated by a filter in-between. its lifespan and being a much more cost-effective alternative to systems that require syringe filters and filter caps.Need more cost-savings proof? Let’s do some more math: The Metrohm IC way Plug in your numbers 1 No. of samples analyzed per year 1000 2 No. of samples a single filter can handle 200 3 No. of filters required per year (divide line 1 by line 2) 5 4 Cost per filter $6.00* $ 5 Yearly filter cost (multiply lines 3 & 4) $30.00* $ 6 No. of filter caps per year 0 7 Cost per filter cap (if applicaple) $0.00 $ Yearly filter cap cost, if applicaple 8 $0.00 $ (multiply lines 6 & 7) 9 Yearly consumables grand total (add lines 5 & 8) $30.00 $*Price listed in USD as of 6/30/2011 With Metrohm IC you can save THOUSANDS each year on filtration consumables alone. Toll-free 866-METROHM (638-7646) 23

CONTACT INFORMATION Contact us anywhere in North America 866-METROHM (638-7646) • www.metrohmna.com Titration • Ion Chromatography • Electrochemistry Process Analytics United States Canada Applikon USA Business Hours: 8:00 a.m. to 7:00 p.m. EST 8:30 a.m. to 5:00 p.m. EST 8:00 a.m. to 5:00 p.m. CST Toll-free: 866-METROHM (638-7646) Toll-free Phone & Fax Local: 813-316-4700 Local: 905-569-0664 Tel: 866-354-2899 Fax: 813-316-4900 Fax: 905-569-0665 Fax: 866-354-2909 Phone/Web: www.metrohmusa.com www.metrohmca.com www.applikonusa.com info@metrohmusa.com info@metrohmca.com info@applikonusa.com Metrohm USA, Inc. Metrohm Canada, Ltd. Applikon Analyzers, Inc. 6555 Pelican Creek Circle 4160 Sladeview Crescent, #6 1701 Northpark Drive, #25 Riverview, FL 33578 Mississauga, ONT L5L 0A1 Kingwood, TX 77339 Address: SPECIALIZED SUPPORT/SERVICE CONTACTS customersupport@metrohmusa.com customersupport@metrohmca.com customersupport@applikonusa.com Customer Support: service@metrohmusa.com service@metrohmca.com service@applikonusa.com Repair: applicationslab@metrohmusa.com applicationslab@metrohmca.com applicationslab@applikonusa.com Applications Help:US & Canada, toll-free: 866-METROHM (638-7646)www.metrohmusa.com • www.metrohmca.com9702.C2.1001 © 2011 Metrohm USA, Inc. Metrohm and design® is a registered trademark of Metrohm Ltd.Product appearance, specifications, and/or prices are subject to change without notice.

Environmental applications brochure

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