Dna Extraction Principles

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Deb put together a wonderful presentation to follow while our 8th graders extracted DNA from strawberries.

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  • Mathur

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  • how to download this file can you plz send copy of slide it's urgent
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  • stupid, not helpful at all
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  • Feel free. A parent volunteer actually put it together for us.
    - Megan
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  • Tank you. It discovered it also downloaded fine for me. Do you mind I use/ adapt it to use with my students? (I am a portuguese teacher, with 9 grade kids)
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Dna Extraction Principles

  1. 1. DNA Extraction Principles How do you get DNA out of a whole organism?
  2. 2. How do you get DNA out of a strawberry? Plant Cell Wall: cellulose and protein matrix Cell membrane: lipids and protein Plant Cell Cytoplasm: proteins etc Nucleus: DNA & protein
  3. 3. What does the detergent and NaCl do to the cell parts? Mashing the berry disrupts the cells and membranes <ul><li>What is in the bag? </li></ul><ul><li>Clumps of whole cells and nuclei </li></ul><ul><li>Cell Membranes: lipids and protein </li></ul><ul><li>Organelles made of protein </li></ul><ul><li>4. Plant Cell Wall: cellulose and protein </li></ul><ul><li>5. DNA bound to protein </li></ul>
  4. 4. What does detergent and NaCl do? <ul><li>Detergent: dissolves the membranes and disrupts proteins. </li></ul><ul><li>2. Na+ ions break apart the protein that is stuck to the DNA. </li></ul>How do we separate the clumps of cells from the small molecules?
  5. 5. Filtering -Clumps of cellular debris get stuck in the filter. -Small molecules flow thru. How do we get the DNA to separate from the pink liquid?
  6. 6. Precipitate the DNA with cold alcohol <ul><li>DNA is not soluble in cold alcohol </li></ul><ul><li>All small molecules stay in solution except DNA </li></ul><ul><li>Long strands of DNA spool onto the stick </li></ul>
  7. 7. Summary <ul><li>1. Break open cells by mashing the fruit. </li></ul><ul><li>2. Dissolve organelles and cell membranes with detergent. </li></ul><ul><li>3. Separate DNA from proteins with salt: </li></ul><ul><ul><li>Na(+) is attracted to (–)DNA so it bumps off the protein. </li></ul></ul><ul><li>4. Filter out the clumps with a coffee filter </li></ul><ul><li>5. DNA precipitates in cold alcohol and is spooled out. </li></ul>
  8. 8. DNA Properties <ul><ul><li>a. Texture – strands of cellular DNA are very long (thus we can spool it) </li></ul></ul><ul><ul><li>b. Solubility – DNA is soluble in water, insoluble in alcohol </li></ul></ul><ul><ul><li>c. pH – ? </li></ul></ul><ul><ul><li>d. Can you see individual </li></ul></ul><ul><ul><li>strands of DNA? </li></ul></ul>

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