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Dna Extraction Principles
Dna Extraction Principles
Dna Extraction Principles
Dna Extraction Principles
Dna Extraction Principles
Dna Extraction Principles
Dna Extraction Principles
Dna Extraction Principles
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Dna Extraction Principles

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Deb put together a wonderful presentation to follow while our 8th graders extracted DNA from strawberries.

Deb put together a wonderful presentation to follow while our 8th graders extracted DNA from strawberries.

Published in: Technology
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  • Mathur

    Send me your mail so I can send it to you
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  • how to download this file can you plz send copy of slide it's urgent
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  • stupid, not helpful at all
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  • Feel free. A parent volunteer actually put it together for us.
    - Megan
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  • Tank you. It discovered it also downloaded fine for me. Do you mind I use/ adapt it to use with my students? (I am a portuguese teacher, with 9 grade kids)
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  • 1. DNA Extraction Principles How do you get DNA out of a whole organism?
  • 2. How do you get DNA out of a strawberry? Plant Cell Wall: cellulose and protein matrix Cell membrane: lipids and protein Plant Cell Cytoplasm: proteins etc Nucleus: DNA & protein
  • 3. What does the detergent and NaCl do to the cell parts? Mashing the berry disrupts the cells and membranes
    • What is in the bag?
    • Clumps of whole cells and nuclei
    • Cell Membranes: lipids and protein
    • Organelles made of protein
    • 4. Plant Cell Wall: cellulose and protein
    • 5. DNA bound to protein
  • 4. What does detergent and NaCl do?
    • Detergent: dissolves the membranes and disrupts proteins.
    • 2. Na+ ions break apart the protein that is stuck to the DNA.
    How do we separate the clumps of cells from the small molecules?
  • 5. Filtering -Clumps of cellular debris get stuck in the filter. -Small molecules flow thru. How do we get the DNA to separate from the pink liquid?
  • 6. Precipitate the DNA with cold alcohol
    • DNA is not soluble in cold alcohol
    • All small molecules stay in solution except DNA
    • Long strands of DNA spool onto the stick
  • 7. Summary
    • 1. Break open cells by mashing the fruit.
    • 2. Dissolve organelles and cell membranes with detergent.
    • 3. Separate DNA from proteins with salt:
      • Na(+) is attracted to (–)DNA so it bumps off the protein.
    • 4. Filter out the clumps with a coffee filter
    • 5. DNA precipitates in cold alcohol and is spooled out.
  • 8. DNA Properties
      • a. Texture – strands of cellular DNA are very long (thus we can spool it)
      • b. Solubility – DNA is soluble in water, insoluble in alcohol
      • c. pH – ?
      • d. Can you see individual
      • strands of DNA?

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