Dna fingerprinting

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Dna fingerprinting

  1. 1.  DNA fingerprinting is the method of extracting a person’s DNA and finding their specific DNA banding pattern. This pattern is unique to every person and depends on the order of nitrogen bases in their DNA.
  2. 2.  The three types of DNA fingerprinting are: RFLP STR Mitochondrial DNA Analysis
  3. 3. 1. Extract the DNA from cells or tissues of the body.2. Add an restriction enzyme that cuts the DNA.3. Put the cut DNA pieces on to a nylon sheet, then place the sheet on a gel and let it soak. (Gel Electrophoresis)4. Add probes that produce the pattern of the DNA fingerprint.
  4. 4. 1. Extract the DNA2. Add radioactive probes. This shows points in the DNA, no person has the probes in the same spot on their DNA.
  5. 5.  Mitochondrial DNA Analysis uses the same steps as RFLP except it uses mitochondrial DNA instead of DNA from the nucleus.
  6. 6.  Find suspects of crimes or eliminate them as a suspect. Identify victims of crime or catastrophe. Identify parentage or family relationships. Diagnose or find cures for inherited disorders.
  7. 7.  There are advantages for every type.1. RFLP was the first type of DNA fingerprinting.2. Mitochondrial analysis is better than RFLP and STR because you can use it on older, degraded DNA.3. STR is faster than RFLP and can be used on a smaller amount of DNA.
  8. 8.  Privacy, if we stored a person’s fingerprint we would have access very personal information. Space, if we started taking DNA we wouldn’t have enough room for it all.
  9. 9.  http://www.ornl.gov/sci/techresources/H uman_Genome/elsi/forensics.shtml http://www.defending.com/dna- fingerprinting-process http://www.odec.ca/projects/2004/fren4 j0/public_html/dna_fingerprinting.htm http://www.accessexcellence.org/RC/A B/BA/DNA_Fingerprinting_Basics.php

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