Cell Boundaries<br />Chapter 6<br />
What are boundaries?<br />We have borders surrounding countries<br />We have boundaries on a playing field<br />Why are bo...
What are the borders of a cell?<br />Cell membrane<br />Thin, flexible barrier<br />Cell wall<br />Plant, algae, fungi, pr...
Cell Membrane<br />What does it do for cell?<br />Controls what goes in and out <br />Regulates molecules moving from one ...
Cell Membrane<br />Lipid bilayer<br />What are lipids?<br />What does bi- mean?<br />What’s a layer?<br />A cell membrane ...
Cell membrane<br />Phospholipids bilayer<br />Made of a negatively charged phosphate “head”<br />PO43-<br />Attracts water...
Other things in the membrane…<br />Proteins embedded in lipid bilayer<br />Carbohydrates attached to proteins<br />So many...
Proteins<br />Proteins help things get across membrane<br />Kinda like a pump<br />
Carbohydrates…<br />Chemical identification cards<br />“ID” card of cell<br />Helps individual cells id each other<br />
Cell Walls<br />Outside cell membrane (does NOT replace membrane!!!)<br />Plants, algae, fungi, prokaryotes<br />Have pore...
Cell walls made of…<br />Protein and carbs<br />Plant cell walls made up of CELLULOSE<br />This is a tough carbohydrate<br...
A biological membrane<br />Cells exist in liquid environments<br />Things need to get in and out of cell<br />Different wa...
Concentration (conc.)<br />Solution<br />Mix of 2 or more substances<br />Solutes<br />Substance dissolved in solution<br ...
Diffusion<br />Particles constantly move<br />Collide randomly<br />Spread out randomly<br />Diffusion is moving from area...
Equilibrium<br />When the conc. Of a system is the same throughout <br />(same conc. on both sides)<br />
What happens when we reach equilibrium?<br />Particles continue moving across membrane but in both directions!***No more c...
If things can cross a membrane we call the membrane PERMEABLE<br />If things canNOT cross a membrane we call the membrane ...
Biological membranes are…<br />SEMI-PERMEABLE<br />A.K.A.<br />Selectively permeable<br />Cell membranes are picky<br />
What’s this have to do with cells?<br />Cell have liquid inside and are found in liquid environment<br />We have substance...
What diffuses across the membrane???<br />Small, uncharged (non-polar) molecules<br />Examples: <br />Carbon dioxide<br />...
Cells are always trying to Maintain Equilibrium by…3 ways…<br />PASSIVE Transport<br />2 types<br />Requires NO energy<br ...
Osmosis<br />Water (H2O) can pass easily through most biological membranes<br />Def: <br />Diffusion of Water through a se...
How Osmosis works…<br />We have water molecules and some other molecules, let’s say sugar<br />If we have more water on on...
Water moves from areas of HIGH conc. To areas of LOW conc.<br />Water moves across till equilibrium is reached<br />Isoton...
Osmotic Pressure<br />Central vacuole fills with water and exerts and out ward pressure on cell membrane and cell wall<br ...
means by which cells keep the concentration of cell cytoplasm or blood at a suitable concentration.</li></li></ul><li>Prob...
Osmotic Pressure<br />Pressure exerted by osmosis on the HYPERTONIC side of a semi-permeable membrane<br />Think about blo...
Why don’t all our cells burst?<br />What are our cells (animal) contained in?<br />Fresh water?<br />No…..blood or other f...
Which is a RBC in isotonic solution?<br />RBC in hypertonic solution?<br />RBC in hypotonic solution?<br />
How do big molecules diffuse across a membrane so quickly if the membrane is selectively permeable?<br />
Problems for diffusion…<br />Things too large (like Glucose!)<br />Charged molecules and Polar molecules<br />Positive/neg...
Facilitated Diffusion<br />What does facilitate mean?<br />Proteins are the extra help<br />“escorts” across the membrane<...
Facilitated Diffusion<br /><ul><li>takes place through proteins, or assemblies of proteins, embedded in the plasma membran...
Facilitated Diffusion (continued)<br />FAST<br />SPECIFIC<br />Still diffusion so we only see it from high concentration t...
What about when we want to go against the concentration?<br />(From low concentration to high?)<br />
What do we need???<br />ENERGY!!!!<br />
ACTIVE transport<br />Process that moves molecules against the concentration gradient<br />Requires ENERGY<br />A protein ...
Build up of Na+ ions on one side of membrane from Na+/K+ pump <br />Now Na+ will flow thru another channel that allows one...
Endocytosis<br />When cells need to take in large material<br />Process of taking material into the cell by process of inf...
Two types of endocytosis:<br />1. Phagocytosis<br />“cell eating”<br />Extensions of cytoplasm surround the particle and p...
Exocytosis<br />Exo- means…<br />Cyto means…<br />Sis means…<br />When cell releases large amounts of material<br />Excret...
Cells are always trying to Maintain Equilibrium by…3 ways…<br />PASSIVE Transport<br />2 types<br />Requires NO energy<br ...
MEMBRANE MAMBO!!!<br />
Membrane Mambo<br />10 minutes to plan<br />2 teams/Cells<br />Nucleus for each team<br />Each member must be assigned a m...
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Cell membrane powerpoint diffusion and osmosis revised

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Transcript of "Cell membrane powerpoint diffusion and osmosis revised"

  1. 1.
  2. 2. Cell Boundaries<br />Chapter 6<br />
  3. 3. What are boundaries?<br />We have borders surrounding countries<br />We have boundaries on a playing field<br />Why are borders important?<br />
  4. 4. What are the borders of a cell?<br />Cell membrane<br />Thin, flexible barrier<br />Cell wall<br />Plant, algae, fungi, prokaryotes<br />Strong supporting layer<br />
  5. 5. Cell Membrane<br />What does it do for cell?<br />Controls what goes in and out <br />Regulates molecules moving from one liquid side of the cell to the other liquid side of the cell<br />Protects<br />Supports<br />
  6. 6. Cell Membrane<br />Lipid bilayer<br />What are lipids?<br />What does bi- mean?<br />What’s a layer?<br />A cell membrane is made of two<br /> layers of lipid molecules<br />
  7. 7. Cell membrane<br />Phospholipids bilayer<br />Made of a negatively charged phosphate “head”<br />PO43-<br />Attracts water because the phosphate is charged (-)<br />Water is a polar , slightly positive ends and slightly negative ends<br />Attached to the phosphate group are 2 fatty acid chains<br />Hydrophobic= don’t like water<br />So the inside of the cell membrane doesn’t let water in but the outside allows cells to be dissolved in aqueous environments<br />
  8. 8.
  9. 9. Other things in the membrane…<br />Proteins embedded in lipid bilayer<br />Carbohydrates attached to proteins<br />So many different molecules in membrane, we call it a “mosaic” of different molecules<br />
  10. 10.
  11. 11. Proteins<br />Proteins help things get across membrane<br />Kinda like a pump<br />
  12. 12. Carbohydrates…<br />Chemical identification cards<br />“ID” card of cell<br />Helps individual cells id each other<br />
  13. 13.
  14. 14. Cell Walls<br />Outside cell membrane (does NOT replace membrane!!!)<br />Plants, algae, fungi, prokaryotes<br />Have pores to allow things in<br />Function: SUPPORT and PROTECT<br />
  15. 15.
  16. 16. Cell walls made of…<br />Protein and carbs<br />Plant cell walls made up of CELLULOSE<br />This is a tough carbohydrate<br />Wood and paper<br />
  17. 17. A biological membrane<br />Cells exist in liquid environments<br />Things need to get in and out of cell<br />Different ways to do this….<br />The Cell Membrane<br />
  18. 18. Concentration (conc.)<br />Solution<br />Mix of 2 or more substances<br />Solutes<br />Substance dissolved in solution<br />Concentration <br />Molarity (M) is # of Moles of a substance per liter of solution<br />6.02 x 1023 “small things” (molecules, atoms, ions, electrons, ions, etc)<br />Moles is a unit for measuring EXTREMELY small things<br />is mass of SOLUTE in a given volume of solution (g/L)<br />What is the concentration of 12 grams of salt dissolved in 3 liters of water?<br />12g/3L= 4g/L<br />
  19. 19. Diffusion<br />Particles constantly move<br />Collide randomly<br />Spread out randomly<br />Diffusion is moving from area of HIGH conc. to area of LOW conc.<br />This is what we call the CONCENTRATION GRADIENT<br />
  20. 20.
  21. 21. Equilibrium<br />When the conc. Of a system is the same throughout <br />(same conc. on both sides)<br />
  22. 22. What happens when we reach equilibrium?<br />Particles continue moving across membrane but in both directions!***No more changes in concentration<br />
  23. 23. If things can cross a membrane we call the membrane PERMEABLE<br />If things canNOT cross a membrane we call the membrane IMPERMEABLE<br />
  24. 24. Biological membranes are…<br />SEMI-PERMEABLE<br />A.K.A.<br />Selectively permeable<br />Cell membranes are picky<br />
  25. 25. What’s this have to do with cells?<br />Cell have liquid inside and are found in liquid environment<br />We have substances (solutes) inside and outside cell<br />Unequal concentrations means we get DIFFUSION!!!<br />B/c diffusion depends on random particle movements, substances diffuse across membranes without requiring the cell to use energy<br />
  26. 26. What diffuses across the membrane???<br />Small, uncharged (non-polar) molecules<br />Examples: <br />Carbon dioxide<br />Oxygen<br />
  27. 27. Cells are always trying to Maintain Equilibrium by…3 ways…<br />PASSIVE Transport<br />2 types<br />Requires NO energy<br />Goes with Conc. Gradient<br />types:<br />SIMPLE DIFFUSION-<br />No protein required<br />Small, uncharged particles<br />FACILITATED DIFFUSION-<br />CHANNEL or CARRIER proteins<br />Trans-membrane protein channel<br />Protein with a specific shape; open-close mechanism<br />Ex. Osmosis and ligand-gated channels<br />ACTIVE Transport<br />1 type<br />REQUIRES Energy<br />Goes Against Conc. Gradient<br />ACTIVE TRANSPORT<br />Involves “transporter” membrane protein and ENERGY (ATP)<br />
  28. 28. Osmosis<br />Water (H2O) can pass easily through most biological membranes<br />Def: <br />Diffusion of Water through a semi-permeable membrane<br />Small molecules of water can sometimes diffuse across easily (no protein needed)<br />AQUAPORIN:<br />What does “aqua” mean???<br />Protein in cell membrane that allows water through (speeds up diffusion of water across the membrane)<br />Type of Facilitated diffusion (more on this later)<br />
  29. 29. How Osmosis works…<br />We have water molecules and some other molecules, let’s say sugar<br />If we have more water on one side of the membrane (HIGH conc. of H2O), then the water will diffuse across…<br />Membrane will let water thru but not sugar <br />Water can move back and forth (not sugar)<br />
  30. 30. Water moves from areas of HIGH conc. To areas of LOW conc.<br />Water moves across till equilibrium is reached<br />Isotonic: <br />when conc. Of both solutions is equal<br />Hypertonic: “above strength”<br />When we begin with more sugar in water<br />More concentrated sugar solution on side A<br />Hypotonic: “below strength”<br />When we end with less sugar <br />Dilute sugar solution on side A<br />
  31. 31.
  32. 32.
  33. 33. Osmotic Pressure<br />Central vacuole fills with water and exerts and out ward pressure on cell membrane and cell wall<br />Cell wall does NOT allow cell to expand past a certain size<br />Osmoregulation<br /><ul><li>Osmoregulation
  34. 34. means by which cells keep the concentration of cell cytoplasm or blood at a suitable concentration.</li></li></ul><li>Problems in Plant Cell<br />
  35. 35. Osmotic Pressure<br />Pressure exerted by osmosis on the HYPERTONIC side of a semi-permeable membrane<br />Think about blowing up a balloon…<br />Can cause serious problems for cell<br />Cell is full of sugar, salts, proteins, molecules<br />Inside of cell is Hypertonic<br />Osmotic pressure should make fresh water go…?<br />In or Out?<br />In<br />Cell will become inflated/swollen<br />Too much cell will burst like a balloon<br />
  36. 36. Why don’t all our cells burst?<br />What are our cells (animal) contained in?<br />Fresh water?<br />No…..blood or other fluids, which are ISOTONIC<br />The conc. Of sugars, salts, proteins and molecules in these fluids is the same as the conc. in the cell<br />Bacteria and plant cells<br />They DO come in contact with fresh water…what do they have PROTECTING them?<br />CELL WALL<br />Prevent cells from expanding even when there’s a lot of osmotic pressure<br />But, cell walls are prone to injuries when there is too much osmotic pressure<br />
  37. 37. Which is a RBC in isotonic solution?<br />RBC in hypertonic solution?<br />RBC in hypotonic solution?<br />
  38. 38.
  39. 39.
  40. 40. How do big molecules diffuse across a membrane so quickly if the membrane is selectively permeable?<br />
  41. 41. Problems for diffusion…<br />Things too large (like Glucose!)<br />Charged molecules and Polar molecules<br />Positive/negative<br />Opposites attract but likes do not…<br />These all present serious problems for things getting across membranes…<br />
  42. 42. Facilitated Diffusion<br />What does facilitate mean?<br />Proteins are the extra help<br />“escorts” across the membrane<br />We call these membrane proteins…<br />Protein channels<br />Carrier proteins<br />LIGAND receptor proteins<br />
  43. 43. Facilitated Diffusion<br /><ul><li>takes place through proteins, or assemblies of proteins, embedded in the plasma membrane</li></ul>Protein channels<br />Carrier proteins<br />LIGAND receptor proteins<br />
  44. 44. Facilitated Diffusion (continued)<br />FAST<br />SPECIFIC<br />Still diffusion so we only see it from high concentration to low<br />Does NOT require energy<br />
  45. 45.
  46. 46. What about when we want to go against the concentration?<br />(From low concentration to high?)<br />
  47. 47. What do we need???<br />ENERGY!!!!<br />
  48. 48. ACTIVE transport<br />Process that moves molecules against the concentration gradient<br />Requires ENERGY<br />A protein pumps small molecules and ions across a cell membrane against the conc. Gradient<br />Direct and Indirect Active transport (see animation)<br />Forms of molecular transport proteins are used to pump small molecules and ions across membrane even against the conc. grad.<br />Direct: every 3 Na+ ions pumped across for every 2 K+ ions<br />Indirect: Build up of ions on one side opens up another channel to shuttle in other molecules (Na+/Glucose channel)<br />Electrochemical gradient<br />membrane potential (cell membrane is negative)<br />Na+ opens build up on the outside of the cell…draws water out of the cell so it doesn’t swell or burst!<br />To pump large molecules and clumps…<br />2 other processes:<br />Exocytosis: exo-means….cyto- means…..-sis means….<br />Endocytosis: endo-means….cyto- means…..-sis means….<br />They can change shape of membrane<br />
  49. 49. Build up of Na+ ions on one side of membrane from Na+/K+ pump <br />Now Na+ will flow thru another channel that allows one glucose in too!<br />Pretty convenient!<br />
  50. 50. Endocytosis<br />When cells need to take in large material<br />Process of taking material into the cell by process of infolding, or pockets, of the cell membrane<br />Pocket breaks loose from cell membrane and forms a vacuole or vesicle inside the cell<br />Two Types…<br />Phagocytosis<br />Pinocytosis<br />
  51. 51.
  52. 52. Two types of endocytosis:<br />1. Phagocytosis<br />“cell eating”<br />Extensions of cytoplasm surround the particle and package it within a food vesicle<br />Cell then engulfs the package<br />Amoebas<br />2. Pinocytosis<br />“cell drinking”<br />When cell needs to take up liquid<br />Tiny pockets in cell membrane form<br />Fill with liquid or many smaller molecules<br />Then pinch off to form vesicles inside of cell<br />
  53. 53.
  54. 54.
  55. 55. Exocytosis<br />Exo- means…<br />Cyto means…<br />Sis means…<br />When cell releases large amounts of material<br />Excretes stuff<br />Membrane of vacuole surrounding particle inside cell fuses with the cell membrane<br />The contents in vacuole are then forced out of the cell<br />We see this in removal of water by contractile vacuoles<br />
  56. 56.
  57. 57.
  58. 58. Cells are always trying to Maintain Equilibrium by…3 ways…<br />PASSIVE Transport<br />2 types<br />Requires NO energy<br />Goes with Conc. Gradient<br />types:<br />SIMPLE DIFFUSION-<br />No protein required<br />Small, uncharged particles<br />FACILITATED DIFFUSION-<br />CHANNEL or CARRIER proteins<br />Trans-membrane protein channel<br />Protein with a specific shape; open-close mechanism<br />Ex. Osmosis and ligand-gated channels<br />ACTIVE Transport<br />1 type<br />REQUIRES Energy<br />Goes Against Conc. Gradient<br />ACTIVE TRANSPORT<br />Involves “transporter” membrane protein and ENERGY (ATP)<br />
  59. 59. MEMBRANE MAMBO!!!<br />
  60. 60. Membrane Mambo<br />10 minutes to plan<br />2 teams/Cells<br />Nucleus for each team<br />Each member must be assigned a molecular role (must be labeled)<br />Membrane protein(s)<br />Molecule A (ex. Glucose)<br />Molecule B (ex. Na+)<br />Molecule C (ex. Water)<br /> Remember molecules are ALWAYS moving!!!<br />MUST act out the following types of membrane transport:<br />Simple Diffusion<br />Osmosis<br />Facilitated diffusion (channel, carrier and ligand)<br />Active transport (direct and indirect)<br />Be prepared to determine the concentration on either side of the membrane<br />Your “Nucleus” must turn in a paper with every ones name and roles<br />Summary of how each scenario will be acted out<br />
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