Speaker:XineYeah,Tang<br />
Rhizopusoryzae producing triacylglycerollipase<br />mono- and diacylglycerol lipase (mdlB) derived from Aspergillusoryzae....
Introduction<br />R. oryzae, which originally produces a 1,3-position-specific triacylglycerol lipase, is one of the most ...
Studies by our group revealed that BSP-immobilized R. oryzae can efficiently catalyze the methanolysis with the addition o...
Mono- and diacylglycerol lipase is an enzyme that hydrolyzes MGandDG, but doesnothydrolyzetriglyceride (TG). Themono-and d...
Materials and methods<br />Two fungal strains, R. oryzae IFO4697 and A. oryzaeniaD300,were used.<br />Reticulated polyuret...
Construction of lipase expression vector and transformation of A. oryzae(skip)<br />
Results and discussion<br />Substrate specificity of mdlB<br />
Effect of water content on methanolysis usingmdlB-producing A. oryzae<br />
Composition of reaction mixtures in methanolysis usingmdlB-producing A. oryzae<br />
Two-step batch methanolysis in PBR<br />
Two-step batch methanolysis in PBR<br />
Two-step batch methanolysis in PBR<br />
Conclusions<br />In this study, we attempted to use a mono- and diacylglycerol lipase to convert residual partial glycerid...
The newly developed mdlB-producing A. oryzaeshowed an effective methanolysis activity toward MG and DG. In the presence of...
Ten repeated-batch methanolysiscycles in the PBR, containing both R. oryzae and mdlBproducing A. oryzae, maintained a high...
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Use of mono- and diacylglycerol lipase as immobilized fungal whole cells to convert residual partial glycerides enzymatically into fatty acid methyl esters

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Use of mono- and diacylglycerol lipase as immobilized fungal whole cells to
convert residual partial glycerides enzymatically into fatty acid methyl esters

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Use of mono- and diacylglycerol lipase as immobilized fungal whole cells to convert residual partial glycerides enzymatically into fatty acid methyl esters

  1. 1. Speaker:XineYeah,Tang<br />
  2. 2. Rhizopusoryzae producing triacylglycerollipase<br />mono- and diacylglycerol lipase (mdlB) derived from Aspergillusoryzae.<br />
  3. 3. Introduction<br />R. oryzae, which originally produces a 1,3-position-specific triacylglycerol lipase, is one of the most studied fungi in lipase-catalyzed methanolysis<br />
  4. 4. Studies by our group revealed that BSP-immobilized R. oryzae can efficiently catalyze the methanolysis with the addition of a certain amount of water , and that a packed-bed reactor (PBR) using BSPimmobilizedR. oryzae can be used for long-term repeated-batch methanolysis, with the methyl ester (ME) content around 80–90% during 10 batch cycles .<br />
  5. 5. Mono- and diacylglycerol lipase is an enzyme that hydrolyzes MGandDG, but doesnothydrolyzetriglyceride (TG). Themono-and diacylglycerollipase-encoding genes have been cloned fromthefilamentous fungi, Penicilliumcamembertii (mdlA) and A. oryzae<br />(mdlB) .<br />
  6. 6.
  7. 7. Materials and methods<br />Two fungal strains, R. oryzae IFO4697 and A. oryzaeniaD300,were used.<br />Reticulated polyurethane foam (Bridgestone Co. Ltd., Osaka, Japan) with a particle voidageofmore than 97% and a pore size of 50 pores per linear inch (ppi) was cut into 6mm×6mm×3mm cuboids for use asBSPs.<br />
  8. 8. Construction of lipase expression vector and transformation of A. oryzae(skip)<br />
  9. 9. Results and discussion<br />Substrate specificity of mdlB<br />
  10. 10. Effect of water content on methanolysis usingmdlB-producing A. oryzae<br />
  11. 11. Composition of reaction mixtures in methanolysis usingmdlB-producing A. oryzae<br />
  12. 12. Two-step batch methanolysis in PBR<br />
  13. 13. Two-step batch methanolysis in PBR<br />
  14. 14. Two-step batch methanolysis in PBR<br />
  15. 15. Conclusions<br />In this study, we attempted to use a mono- and diacylglycerol lipase to convert residual partial glycerides efficiently into their corresponding MEs. The newly developed mdlB-producing A.<br />
  16. 16. The newly developed mdlB-producing A. oryzaeshowed an effective methanolysis activity toward MG and DG. In the presence of 5% water, the methanolysis of intermediate products using mdlB-producing A. resulting in less than 0.1% of MG and DG.<br />
  17. 17. Ten repeated-batch methanolysiscycles in the PBR, containing both R. oryzae and mdlBproducing A. oryzae, maintained a high ME content of over 90% with MG and DG at 0.08–0.69 and 0.22–1.45%, respectively. These results indicate that the use of mdlB-producing A. oryzaeallows maintaining MG and DG at low levels during long-term repeatedbatchmethanolysis. We thus conclude that the proposed method is effective for improving enzymatic biodiesel production.<br />

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