Marius L. Matache*, Iulia G. David, Carmen Hura, Laurenţiu Rozylowicz, Gabriel Chişamera, Diana Onose   Bioaccumulation of...
<ul><li>OCP concentration in levels of the trophic chain; </li></ul><ul><ul><li>Molluscs, </li></ul></ul><ul><ul><li>Fish,...
<ul><li>Lower Prut Floodplain Natural Park - recently established protected area, SE border Romania - Moldova; </li></ul>S...
Study area <ul><li>Prut River: more than 900 km, water from a 27,000 km 2  watershed; </li></ul><ul><li>Agricultural  and ...
Sampling&Methods <ul><li>Samples processing:  petroleum ether extraction, Na 2 SO 4  anh., fluorysil column purification, ...
DDTs in  Sinanodonta woodiana <ul><li>Anoxic environment – slow metabolisation; </li></ul><ul><li>alpha- and beta-HCH - ab...
DDTs in bird eggs&feathers <ul><li>DDE, DDD - highest concentration in  Sturnus vulgaris  eggs ; </li></ul><ul><li>Frugivo...
DDTs in bird eggs&feathers <ul><li>Anas sp., Fulica atra  eggs – lower concentration than in Danube Delta*; </li></ul><ul>...
HCHs in bird eggs and feathers <ul><li>Lower levels of concentration compared with the DDTs; </li></ul><ul><li>gama-HCH: 3...
HCHs in bird eggs and feathers <ul><li>Gamma-HCH/ΣHCH in feathers   50% in all the samples and   90% in 1/3 of samples. ...
Bird eggs and feather comparison 7.41 3.62 ΣHCH 33.98 42.76 ΣHCH 5.0 3.5 Gamma-HCH 6.4 27.0 Gamma-HCH 18.7 13.8 DDT 16.0 8...
DDTs organotropism in fish <ul><li>Gills – most exposed organ; </li></ul>DDD DDE DDT
HCHs organotropism in fish <ul><li>Alpha-, Gamma-HCH – preferentially accumulate in gills; </li></ul><ul><li>Muscle concen...
Conclusions <ul><li>DDT group – main OCP in the wetlands ecosystems from the Lower Prut floodplain; </li></ul><ul><li>OCP ...
Acknowledgements <ul><li>Romanian Ministry of Education, Research, Youth and Sport through the PN II project no. 32111/200...
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Matache Chemistry and Life

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Paper presented at the 5th Meeting on Chemistry and Life, Brno (Czech Republic), September 2011

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Matache Chemistry and Life

  1. 1. Marius L. Matache*, Iulia G. David, Carmen Hura, Laurenţiu Rozylowicz, Gabriel Chişamera, Diana Onose Bioaccumulation of some organochlorine pesticides in biological samples from the Lower Prut Floodplain Natural Park *corresponding author, e-mail: [email_address]
  2. 2. <ul><li>OCP concentration in levels of the trophic chain; </li></ul><ul><ul><li>Molluscs, </li></ul></ul><ul><ul><li>Fish, </li></ul></ul><ul><ul><li>Birds (eggs and feathers) samples; </li></ul></ul><ul><li>DDTs&HCHs organotropism in fish; </li></ul>Study objectives
  3. 3. <ul><li>Lower Prut Floodplain Natural Park - recently established protected area, SE border Romania - Moldova; </li></ul>Study area <ul><li>More than 8,000 ha, significant resources of wildlife&habitats of communitarian importance </li></ul><ul><li>two European Natura 2000 sites: ROSCI0105 and ROSCI0213; </li></ul>
  4. 4. Study area <ul><li>Prut River: more than 900 km, water from a 27,000 km 2 watershed; </li></ul><ul><li>Agricultural and industrial pollution sources; </li></ul><ul><li>DDT and metabolites, HCH (alpha, beta, gamma, delta); </li></ul><ul><li>Romania – history of OCP production; </li></ul><ul><li>Birds: non-invasive sampling; </li></ul>
  5. 5. Sampling&Methods <ul><li>Samples processing: petroleum ether extraction, Na 2 SO 4 anh., fluorysil column purification, hexane : ethylic ether extraction; </li></ul><ul><li>OCP determination: GC-ECD; </li></ul><ul><ul><li>AOC-20i: Injection volum: 1.0 µl; </li></ul></ul><ul><ul><li>Column: Temperature: 120 ºC; Column Information (Rtx-CLPesticides); </li></ul></ul><ul><ul><li>SPL 1: Temperature: 250 ºC; Injection Mode: Split; Sampling Time: 1.00 min.; Carrier Gas: He; </li></ul></ul><ul><ul><li>ECD (detector) : Temperatura: 310.0 ºC; Makeup Gas: N2/Air; </li></ul></ul>
  6. 6. DDTs in Sinanodonta woodiana <ul><li>Anoxic environment – slow metabolisation; </li></ul><ul><li>alpha- and beta-HCH - absent; </li></ul><ul><li>Delta – main isomer; </li></ul>
  7. 7. DDTs in bird eggs&feathers <ul><li>DDE, DDD - highest concentration in Sturnus vulgaris eggs ; </li></ul><ul><li>Frugivore - pollution source located in the agricultural ecosystems nearby; </li></ul><ul><li>DDT – maximum values for Anas platyrynchos; </li></ul><ul><li>DDE – dominant in eggs; </li></ul><ul><li>DDT/(DDE+DDD)  1 in 12/17 samples; </li></ul><ul><li>In 5/17 samples – max 1.1; </li></ul>eggs
  8. 8. DDTs in bird eggs&feathers <ul><li>Anas sp., Fulica atra eggs – lower concentration than in Danube Delta*; </li></ul><ul><li>Feathers - DDT is dominant – slower metabolisation ; </li></ul><ul><li>DDT/(DDE+DDD) - 8/23 samples  1 – max 1.5; </li></ul>*Aurigi et al., 2000, Sci, Tot. Env. feathers
  9. 9. HCHs in bird eggs and feathers <ul><li>Lower levels of concentration compared with the DDTs; </li></ul><ul><li>gama-HCH: 3.5 ng/g in Larus argentatus and 27.4 ng/g in Sturnus vulgaris ; </li></ul><ul><li>ΣHCH : 42.8 ng/g in Anas sp.; </li></ul><ul><li>alpha-, beta- and delta- HCH occasional and at very low levels of concentration; </li></ul>eggs
  10. 10. HCHs in bird eggs and feathers <ul><li>Gamma-HCH/ΣHCH in feathers  50% in all the samples and  90% in 1/3 of samples. </li></ul><ul><li>Lindane use instead of technical HCH; </li></ul>feathers
  11. 11. Bird eggs and feather comparison 7.41 3.62 ΣHCH 33.98 42.76 ΣHCH 5.0 3.5 Gamma-HCH 6.4 27.0 Gamma-HCH 18.7 13.8 DDT 16.0 87.6 DDT 6.1 4.7 DDD 6.4 39.9 DDD 12.9 7.9 DDE 11.5 124.5 DDE Feathers Eggs Larus argentatus Feathers Eggs Anas sp.
  12. 12. DDTs organotropism in fish <ul><li>Gills – most exposed organ; </li></ul>DDD DDE DDT
  13. 13. HCHs organotropism in fish <ul><li>Alpha-, Gamma-HCH – preferentially accumulate in gills; </li></ul><ul><li>Muscle concentration – homogenous; </li></ul><ul><li>Beta, Delta – not significant; </li></ul>Alpha Gamma
  14. 14. Conclusions <ul><li>DDT group – main OCP in the wetlands ecosystems from the Lower Prut floodplain; </li></ul><ul><li>OCP levels of concentration  health threatening levels from literature; </li></ul><ul><li>Both DDTs and HCHs – gills accumulation; </li></ul><ul><li>OCP elimination (eggs laying vs. feather excretion) depends on each species characteristics; </li></ul>
  15. 15. Acknowledgements <ul><li>Romanian Ministry of Education, Research, Youth and Sport through the PN II project no. 32111/2008. </li></ul><ul><li>Dr. Marius Matache was supported by the strategic grant POSDRU/89/1.5/S/58852, Project „Postdoctoral programme for training scientific researchers” cofinanced by the European Social Found within the Sectorial Operational Program Human Resources Development 2007 – 2013. </li></ul>

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