• Share
  • Email
  • Embed
  • Like
  • Save
  • Private Content
It's all about Science!
 

It's all about Science!

on

  • 45 views

Chemistry may be an individual science but it is related to other sciences to like Biology. Today's post includes an experiment we did in Biology class. We learned how to extract DNA from strawberries ...

Chemistry may be an individual science but it is related to other sciences to like Biology. Today's post includes an experiment we did in Biology class. We learned how to extract DNA from strawberries and it was really exciting, interesting and easy! So, I made a video for you, describing how you can extract strawbbery DNA by yourselves. I also added some pictures from the day we did the experiment in class in order to make it easier for you to understand the whole process. Good luck!

Statistics

Views

Total Views
45
Views on SlideShare
44
Embed Views
1

Actions

Likes
0
Downloads
0
Comments
0

1 Embed 1

http://hcoonamatata.blogspot.gr 1

Accessibility

Categories

Upload Details

Uploaded via as Microsoft PowerPoint

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

    It's all about Science! It's all about Science! Presentation Transcript

    • from strawberries
    •  1 resealable plastic bag  Strawberries (fresh or frozen)  2 teaspoons of dish detergent  1 teaspoon of salt  ½ cup of deionized water  2 plastic cups  Filtering apparatus  Ice cold 90 percent rubbing alcohol  1 wooden popsicle stick or plastic coffee stirrer  Gloves  1 vial
    •  Pull off any green leaves on the strawberries.  Put some strawberries into the plastic bag, remove the air and seal it.  Gently smash the strawberries and crush them completely. This starts to break open the cells and release the DNA.
    •  In a plastic cup make your buffer solution: mix together the deionized water, the salt and the detergent.  Add 2 teaspoons of the buffer solution into the bag with the strawberries. This will further break open the cells.  Reseal the bag and continue smashing the strawberries gently for a while.
    •  Place the coffee filter inside the other plastic cup.  Open the bag and pour the strawberry liquid into the filter. You can gently squeeze the remaining liquid into the cup.  Put approximately 4 ml of the liquid into the vial.
    •  Tilt the vial a little bit and then pour slow and carefully about 16 ml of cold rubbing alcohol (until it reaches 20 ml). Do NOT mix or stir. You have just isolated the DNA from the rest of the material contained in the cells of the strawberry.  Within a few seconds, watch for the development of a white cloudy substance (DNA) in the top layer above the strawberry extract layer.  Pick up the DNA using a plastic coffee stirrer or a wooden stick.
    • This is your DNA…
    • You can also save the DNA you extracted into a small vial containing alcohol. In the vial with the strawberry liquid and the alcohol, DNA will continue appearing and if you shake it a little bit, it will have a smooth reddish color.
    • You can keep both vials in normal atmosphere conditions without damaging the DNA. Just have in mind to change the alcohol after a few years!