Principles of Cellular and Molecular ImmunologyDocument Transcript
Universitat de les
UIB Illes Balears
Master in Microbiology
2006-2007 Academic Year
Course title: Principles of Cellular and Molecular Immunology
Course code: a cumplimentar por el Centro de Tecnologías de la Información
Type of course: Optional
Level of course: Postgraduate
Year of study: First
Timetable: See the timetable for the Master in Microbiology programme
Language of instruction: Spanish/Catalan, reading comprehension skills in
Name: Dr. Sebastián Albertí Serrano Contact: email@example.com
A solid background (at the bachelor’s degree level) in biochemistry, molecular biology,
genetics, cellular biology, microbiology, plant-animal physiology and chemistry
Number of ECTS credits 5
Number of classroom hours: 40
Independent study hours: 85
The molecular and cellular aspects of immune reactions. Integration of the immune
response in organisms.
E1- Become conversant with the components of the immune system and the molecular
and cellular bases of the immune response, as well as the integrated response of the
immune system in organisms and its role in different pathologies
E2- Become familiar with and adept at handling various immunological techniques
G1- Acquire an integrated view of microorganisms, their biological properties and their
roles and applications in the fields of ecology, health, industry, agriculture and
G2- To enhance prior knowledge of microbiology and the elements involved in
developing and/or applying ideas at the research level as well as in other areas
1. Introduction to the immune system. Innate and adaptive immunity. Immune system
cells. Soluble mediators in immunity. Antigens. Immune responses. Inflammation.
2. The complement. Introduction. Complement activation. Complement receptors.
Biological effects of the complement.
3. Cells that intervene in the immune response. Markers, lymphocytes, mononuclear
phagocytes, antigen-presenting cells, polymorphonuclears, mast cells and platelets.
4. Cell migration and inflammation. Cell migration patterns. Intercellular adhesion
molecules. Cell migration mechanisms. Inflammation
5. Antibodies and their receptors. Introduction. Antibody structure. Antibody classes
and subclasses. Antibody functions. Antibody receptors.
6. The T cell receptor and the Major Histocompatibility Complex.
7. Generating diversity. Theories on antibody synthesis. Immunoglobulin variability.
Immunoglobulin gene recombination. Somatic mutations. Heavy-chain constant
region gene. Immunoglobulin synthesis. T cell antigen receptor genes.
8. Recognising antigens. Antigen-antibody union. Antibody affinity and specificity. T
cell antigen recognition. Antigen processing and presentation.
9. Cell collaboration in antibody response. Antigen presentation in T cells. T and B cell
interactions. Cytokine actions on B and T cells. In vivo antibody responses.
10. Cell mediated immune reactions. Cytokines and their receptors. T cell independent
mechanisms. T cell and T cell dependent mediated responses. Cell-mediated
cytotoxicity. The role of macrophages in immune responses. Immunopathology.
11. Hypersensitivity phenomena. Type I hypersensitivity. Type II hypersensitivity. Type
III hypersensitivity. Type IV hypersensitivity.
12. Immunopathology. Primary immunodeficiencies. Secondary immunodeficiencies.
1. Antibody production, purification, identification and quantification. Electrophoresis.
Affinity chromatography. Western blot. Immunodiffusion.
2. Antigen-antibody reaction. Antigen quantification. ELISA
Methodology and student workload
1. Teaching method: Classroom sessions
Classroom/independent work: 25/60
Type of group: whole group
2. Teaching method: Laboratory
Classroom/independent work: 10/0
Type of group: pairs
3. Teaching method: Tutorials
Classroom/independent work: 2/0
Type of group: groups of four
4. Teaching method: Theoretical study
Classroom/independent work: 0/25
Type of group: individual
5. Teaching method: Evaluation
Classroom/independent work: 3/0
Type of group: individual
Assessment instruments, criteria and learning agreement
Knowledge of the components of the immune system and the molecular and cellular
bases of the immune response, as well as a grasp of the integrated response of an
organism’s immune system and its role in diverse pathologies. Familiarity with and
capacity to handle different immunological techniques. Students must attend practical
sessions and pass the final exam to earn a passing grade in the course.
A written exam on the course’s theoretical and practical content
A written exam on theoretical and practical course content: 75%
On-going assessment of laboratory notebook: 15%
On-going assessment of lecture classes: 10%
Assessment based on a learning agreement: No
Independent study material and recommended reading
Bibliography, resources and annexes
Inmunología. 5a edición Roitt, I., J. Brostoff, and D. Male. 2000. Harcourt-Mosby,
London. ISBN 84-8174-497-2.
Inmunología celular y molecular, 4a. edición. A.K. Abbas, A.H. Lichtman and
J.S.Pober. 2002. Interamericana-McGraw Hill. ISBN 84-486-0405-9.
Immunology: A Short Course. Benjamini, E., Coico, R., Sunshine, G. Wiley-Liss, ISBN
Biología y patología del sistema inmune. 2a edición. J.R. Regueiro, C. López. 1997.
Editorial Médica Panamericana, Madrid. ISBN 84-7903-403-3.
Molecular biology en Medicina". T.M. Cox, J. Sinclair. 1997. Editorial Médica
Panamericana, Madrid. ISBN 84-7903-412-2.
JOURNALS (available on-line at the UIB library)
CELL. Cell Press. Cambridge.
EUROPEAN JOURNAL OF IMMUNOLOGY. Springer Verlag. New York.
INVESTIGACION Y CIENCIA. Prensa Científica, S.A. Barcelona.
IMMUNITY. Rockefeller University Press. New York.
IMMUNOLOGY TODAY. Rockefeller University Press. New York.
JOURNAL OF IMMUNOLOGY. Rockefeller University Press. New York.
LA RECHERCHE. Societé des Éditions Scientifiques. Paris.
MUNDO CIENTIFICO.Ed.Fontalba,S.A. Barcelona.
NATURE. McMillan Co., Hampshire.
SCIENCE. American Association for the Advancement of Science, Washington.
SCIENTIFIC AMERICAN. Science American Inc.,New York.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES.National
Academy of Sciences of USA. Washington.
TRENDS IN IMMUNOLOGY.Elsevier Trends Journals. New York.
Link to the course teaching guide