Principles of Cellular and Molecular Immunology


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Principles of Cellular and Molecular Immunology

  1. 1. Universitat de les UIB Illes Balears Master in Microbiology COURSE DESCRIPTION 2006-2007 Academic Year Technical information Course Course title: Principles of Cellular and Molecular Immunology Course code: a cumplimentar por el Centro de Tecnologías de la Información Type of course: Optional Level of course: Postgraduate Year of study: First Semester: Second Timetable: See the timetable for the Master in Microbiology programme Language of instruction: Spanish/Catalan, reading comprehension skills in English required Lecturers Supervising lecturer Name: Dr. Sebastián Albertí Serrano Contact: Other lecturers Name: Contact: Name: Contact: Name: Contact: Name: Contact: Prerequisites: A solid background (at the bachelor’s degree level) in biochemistry, molecular biology, genetics, cellular biology, microbiology, plant-animal physiology and chemistry Number of ECTS credits 5 Number of classroom hours: 40 Independent study hours: 85 Description: The molecular and cellular aspects of immune reactions. Integration of the immune response in organisms. Course competences Specific: E1- Become conversant with the components of the immune system and the molecular and cellular bases of the immune response, as well as the integrated response of the immune system in organisms and its role in different pathologies E2- Become familiar with and adept at handling various immunological techniques Generic: G1- Acquire an integrated view of microorganisms, their biological properties and their roles and applications in the fields of ecology, health, industry, agriculture and biotechnology
  2. 2. G2- To enhance prior knowledge of microbiology and the elements involved in developing and/or applying ideas at the research level as well as in other areas Course contents THEORETICAL CONTENT 1. Introduction to the immune system. Innate and adaptive immunity. Immune system cells. Soluble mediators in immunity. Antigens. Immune responses. Inflammation. Vaccination. Immunopathology. 2. The complement. Introduction. Complement activation. Complement receptors. Biological effects of the complement. 3. Cells that intervene in the immune response. Markers, lymphocytes, mononuclear phagocytes, antigen-presenting cells, polymorphonuclears, mast cells and platelets. 4. Cell migration and inflammation. Cell migration patterns. Intercellular adhesion molecules. Cell migration mechanisms. Inflammation 5. Antibodies and their receptors. Introduction. Antibody structure. Antibody classes and subclasses. Antibody functions. Antibody receptors. 6. The T cell receptor and the Major Histocompatibility Complex. 7. Generating diversity. Theories on antibody synthesis. Immunoglobulin variability. Immunoglobulin gene recombination. Somatic mutations. Heavy-chain constant region gene. Immunoglobulin synthesis. T cell antigen receptor genes. 8. Recognising antigens. Antigen-antibody union. Antibody affinity and specificity. T cell antigen recognition. Antigen processing and presentation. 9. Cell collaboration in antibody response. Antigen presentation in T cells. T and B cell interactions. Cytokine actions on B and T cells. In vivo antibody responses. 10. Cell mediated immune reactions. Cytokines and their receptors. T cell independent mechanisms. T cell and T cell dependent mediated responses. Cell-mediated cytotoxicity. The role of macrophages in immune responses. Immunopathology. 11. Hypersensitivity phenomena. Type I hypersensitivity. Type II hypersensitivity. Type III hypersensitivity. Type IV hypersensitivity. 12. Immunopathology. Primary immunodeficiencies. Secondary immunodeficiencies. PRACTICAL CONTENT 1. Antibody production, purification, identification and quantification. Electrophoresis. Affinity chromatography. Western blot. Immunodiffusion. 2. Antigen-antibody reaction. Antigen quantification. ELISA Methodology and student workload 1. Teaching method: Classroom sessions Classroom/independent work: 25/60 E-learning: yes Type of group: whole group 2. Teaching method: Laboratory Classroom/independent work: 10/0 E-learning: no Type of group: pairs 3. Teaching method: Tutorials Classroom/independent work: 2/0 E-learning: no Type of group: groups of four 4. Teaching method: Theoretical study
  3. 3. Classroom/independent work: 0/25 E-learning: yes Type of group: individual 5. Teaching method: Evaluation Classroom/independent work: 3/0 E-learning: no Type of group: individual Assessment instruments, criteria and learning agreement Assessment criteria: Knowledge of the components of the immune system and the molecular and cellular bases of the immune response, as well as a grasp of the integrated response of an organism’s immune system and its role in diverse pathologies. Familiarity with and capacity to handle different immunological techniques. Students must attend practical sessions and pass the final exam to earn a passing grade in the course. Assessment instruments: A written exam on the course’s theoretical and practical content Laboratory notebook Lecture classes Grading criteria: A written exam on theoretical and practical course content: 75% On-going assessment of laboratory notebook: 15% On-going assessment of lecture classes: 10% Assessment based on a learning agreement: No Independent study material and recommended reading Bibliography, resources and annexes BOOKS Inmunología. 5a edición Roitt, I., J. Brostoff, and D. Male. 2000. Harcourt-Mosby, London. ISBN 84-8174-497-2. Inmunología celular y molecular, 4a. edición. A.K. Abbas, A.H. Lichtman and J.S.Pober. 2002. Interamericana-McGraw Hill. ISBN 84-486-0405-9. Immunology: A Short Course. Benjamini, E., Coico, R., Sunshine, G. Wiley-Liss, ISBN 0-471-34890-2. Biología y patología del sistema inmune. 2a edición. J.R. Regueiro, C. López. 1997. Editorial Médica Panamericana, Madrid. ISBN 84-7903-403-3. Molecular biology en Medicina". T.M. Cox, J. Sinclair. 1997. Editorial Médica Panamericana, Madrid. ISBN 84-7903-412-2. JOURNALS (available on-line at the UIB library) CELL. Cell Press. Cambridge. EUROPEAN JOURNAL OF IMMUNOLOGY. Springer Verlag. New York. INVESTIGACION Y CIENCIA. Prensa Científica, S.A. Barcelona. IMMUNITY. Rockefeller University Press. New York. IMMUNOLOGY TODAY. Rockefeller University Press. New York. JOURNAL OF IMMUNOLOGY. Rockefeller University Press. New York. LA RECHERCHE. Societé des Éditions Scientifiques. Paris. MUNDO CIENTIFICO.Ed.Fontalba,S.A. Barcelona.
  4. 4. NATURE. McMillan Co., Hampshire. SCIENCE. American Association for the Advancement of Science, Washington. SCIENTIFIC AMERICAN. Science American Inc.,New York. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES.National Academy of Sciences of USA. Washington. TRENDS IN IMMUNOLOGY.Elsevier Trends Journals. New York. Link to the course teaching guide