L + - +/- Tumorigenic L Eventually + + + Slowly progressive L - - - Latent L - - <<+ Persistent L +/- + + Chronic S - + + Inapparent S <3 wks + + + Acute Duration of infection Signs/ symptoms Cell death Infectious progeny
Recognition of UV-irradiated extracts of HSV-1(KOS)-infected cells by cornea-specific CD4+ T cell clones . Cornea-reactive T cell clones (C1-6 and C1-15) or the OVA-specific clone O3 (2 x~ 10 4 cells per well) were stimulated with UV-irradiated extracts of HSV-1-infected or uninfected Vero cells in the presence of -irradiated syngeneic BALB/c spleen cells (5 x~ 10 5 cells per well). Proliferation was assessed after 2 days by 16 to 18 hours of exposure to 1 µCi of [ 3 H]thymidine ([ 3 H]TdR) and is expressed as mean counts per minute (cpm) ± SEM of triplicate cultures.
Dose-dependent stimulation of cornea-specificCD4 + T cell clones by HSV UL6-(299-314) peptide. CD4 + T cell clones (C1-6 and C1-15) (2 x~ 10 4 cells per well) were incubated with the indicated peptides (0.2 µM) in the presence of irradiated syngeneic BALB/c spleen cells (5 x~ 10 5 cells per well): , p292-308 (IgG2a b )closed square; , p299-314 (UL6) open square; , p200-222 (MMTV).
Analysis of mRNA levels of cytokines 24 h following infection of astrocytes with a neurotropic (MHV-A59) and a nonneurotropic (MHV-2) virus compared with an uninfected control culture. The blots of mouse cytokine array assays are shown. The cytokine key is as follows: A, colony-stimulating factor granulocyte; B, gamma interferon; C, IL-1; D, IL-1ß; E, IL-2; F, IL-3; G, IL-4; H, IL-5; I, IL-6; J, IL-7; K, IL-9; L, IL-10; M, IL-11; N, IL-12 p35; O, IL-12 p40; P, IL-13; Q, IL-15; R, IL-16; S, IL-17; T, IL-18; U, lymphotoxin B; V, TNF-; W, TNF-ß; X, GAPDH; Y, ß-actin; Z, bacterial plasmid (pUC18).
Functional evaluation of Tat transactivation (A) expression vectors encoding the isogenic C-Tat proteins. Differences within the dicysteine motif of these vectors are highlighted.. (B) Transactivation of LTR-driven GFP expression by different Tat vectors in 293 cells. (C) Transactivation of LTR-driven SEAP expression by different Tat vectors in 293 cells. SEAP in the culture medium was quantified on day 1 (open bars) and day 3 (filled bars). (D) Rescue of the Tat-defective virus by isogenic C-Tat proteins. HLM-1 cells were transfected with different C-Tat variant expression vectors. Culture supernatants were collected on days 1, 3, 5, and 7 following transfection, and p24 levels in the culture supernatants were determined. Results of experiments using samples from day 3 are presented; similar results were observed for samples from other days. Abs, absorbance; -VE, parental vector. Contains integrated HIV with Tat defect Secreted AP
Monocyte migration induced by isogenic Tat proteins . f-MLP peptide was used as a positive control at 10 -7 and 10 -8 M concentrations. Tat proteins were used at concentrations of 100 and 20 ng/ml (12 and 2.4 nM, respectively) as indicated. No grad, wells with 100 ng of CC-Tat protein/ml in both the compartments. Differences in the numbers of monocytes that migrated with Tat-CC and Tat-CS were statistically significant Taxis assay: membrane with monocytes on one side and test protein on other Count cells on filter