PII: S0022-1759(98)00158-6
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PII: S0022-1759(98)00158-6 PII: S0022-1759(98)00158-6 Document Transcript

  • Journal of Immunological Methods 220 Ž1998. 179–188 A yeast surface display system for the discovery of ligands that trigger cell activation Bryan K. Cho a , Michele C. Kieke a , Eric T. Boder b, K. Dane Wittrup b, David M. Kranz a,) a Department of Biochemistry, UniÕersity of Illinois, 600 S. Mathews, Urbana, IL 61801, USA b Department of Chemical Engineering, UniÕersity of Illinois, 600 S. Mathews, Urbana, IL 61801, USA Received 1 August 1998; accepted 19 August 1998 Abstract Opposing cells often communicate signalling events using multivalent interactions between receptors present on their cell surface. For example, T cells are typically activated when the T cell receptor ŽTCR. and its associated costimulatory molecules are multivalently engaged by the appropriate ligands present on an antigen presenting cell. In this report, yeast expressing high cell-surface levels of a TCR ligand Ža recombinant antibody to the TCR Vb domain. were shown to act as ‘pseudo’ antigen presenting cells and induce T cell activation as monitored by increased levels of CD25 and CD69 and by downregulation of cell surface TCR. Similar levels of T cell activation could occur even when a 30-fold excess of irrelevant yeast was present, suggesting that such a yeast display system, by virtue of its ability to present ligands multivalently, may be used in highly sensitive procedures to identify novel polypeptides that interact multivalently with cell surface receptors and thereby trigger specific cellular responses. q 1998 Elsevier Science B.V. All rights reserved. Keywords: Yeast display; scFv; T cell activation; Antigen presentation; Biological screening 1. Introduction Fv antibody domains ŽscFv. 2 that bind to tumor-as- sociate antigens ŽWinter et al., 1994.. It would be Display systems have typically been used to dis- advantageous to develop a system that not only cover novel molecules that bind to a specific ligand. identifies polypeptides that bind to ligands, but that For instance, phage display has been used in lieu of also elicit defined cellular responses including apop- B cell hybridoma technology to generate single-chain tosis, gene expression, cytokinesis, secretion, or en- try into the cell cycle. Many of these responses are the culmination of multiple amplification events that follow ligation of cell surface receptors. By exploit- Abbreviations: scFv, single-chain Fv; TCR, T cell receptor; ing these amplification processes, it is possible, in RAG, recombination activating gene; AGA-HA, Aga2-hemag- principle, to greatly enhance the sensitivity with glutinin; 7AAD, 7-aminoactinomycin D; FITC, 5-aminofluorescein isothiocyanate; MHC, major histocompatibility complex which displayed polypeptides are selected. ) Corresponding author. Tel.: q1-217-244-2821; Fax: q1-217- To activate many cellular responses, high valency 244-5858; E-mail: d-kranz@uiuc.edu interactions between receptors on one cell with com- 0022-1759r98r$ - see front matter q 1998 Elsevier Science B.V. All rights reserved. PII: S 0 0 2 2 - 1 7 5 9 Ž 9 8 . 0 0 1 5 8 - 6
  • 180 B.K. Cho et al.r Journal of Immunological Methods 220 (1998) 179–188 plementary molecules on another cell are important cells even in the presence of a great excess of Žreviewed in Metzger, 1992; Singer, 1992.. For ex- unmodified yeast cells, suggesting that this system ample, stimulation of proliferative activity of a rest- may be suitable for screening ‘libraries’ for bioactive ing T cell requires multiple interactions at the inter- peptides or proteins ŽPetsko, 1996.. face of the T cell and antigen presenting cell ŽJorgensen et al., 1992; Allison and Krummel, 1995.. These interactions not only involve different proteins 2. Materials and methods on the T cell surface and their respective polypeptide ligands on the antigen presenting cell, but they typi- 2.1. Mice cally require multivalent clustering of the T cell receptor ŽValitutti et al., 1995; Scharenberg and Kinet, 1996; Reich et al., 1997.. Because of this To produce the 2C TCRrrecombination activat- circumstance, phage display systems which express ing gene ŽRAG.-1yry strain ŽH-2 b . of mice, 2C few molecules per virion have a limited capacity to TCR transgenic mice were bred with RAG-1 knock- induce many cellular responses. Although a recent out mice and the F1 generation was backcrossed to report describes a modification of phage display to RAG-1yry mice ŽManning et al., 1997.. 2C detect receptor activation through dimerized phage TCRrRAGyry mice contain ) 95% CD8q1B2q particles ŽZaccolo et al., 1997., dimerization is not peripheral T cells and lack B cells and T cells of any always sufficient. other specificity. Mice were maintained in barrier In order to design a system that would allow for cages at the University of Illinois animal care facil- rapid screening of polypeptides that trigger cell acti- ity. In the studies presented here, spleens from 2C vation, we have explored the use of yeast cell surface TCRrRAGyry mice were isolated, red blood cells display. In contrast to other display systems, yeast were lysed, and splenocytes were used without fur- offers the advantage of protein folding pathways and ther enrichment. codon usage that are closely related to mammalian cells, thereby eliminating library biases that might be 2.2. Generation of scFÕ-yeast encountered in phage or bacterial display. Yeast also expresses as many as 10 5 fusion proteins per cell at The scFv-KJ16 gene ŽCho et al., 1995., including the yeast surface ŽBoder and Wittrup, 1997., poten- a 3X c-myc epitope tag, was cloned as a downstream tially allowing multivalent interactions to occur be- fusion to the yeast Aga2 gene in the yeast surface tween the yeast and target cell. This system was first display vector pCT202 ŽBoder and Wittrup, 1997.. used to select for higher affinity antibody molecules This construct was transfected into the S. cereÕisiae ŽscFv. against the hapten fluorescein ŽBoder and strain EBY100 Ž trp1 leu2 D1 his3D 200 pep4::HIS2 Wittrup, 1997.. We have also selected for higher prbD1.6R can1 GAL ŽBoder and Wittrup, 1997.. and affinity scFv that recognize protein antigens such as these yeast were termed scFv-yeast. Yeast that only the TCR from a library of scFv generated by random express the Aga2 gene linked to a hemagglutinin mutagenesis ŽKieke et al., 1998.. In this report, a epitope tag Žtermed AGA-HA yeast. were prepared model system is used to demonstrate that yeast are by transfecting EBY100 cells with pCT202, which capable of inducing biological activity by virtue of does not contain a scFv gene. Individual colonies of multivalently presented polypeptides that, as the transfected yeast were grown overnight in Trpy monomers, do not trigger cell activation. Specifi- media containing glucose and harvested in log phase cally, we express a single-chain Fv domain of an ŽOD600 s 0.5–1.5., then grown an additional ; 10 anti-Vb8 antibody ŽKJ16. on the surface of yeast, to 15 h in Trpy media containing galactose in order and show that these yeast Žtermed scFv-yeast. stimu- to induce expression of the fusion protein gene, late naive T cells to express ‘activation’ markers which was cloned behind a GAL1 promoter. Yeast ŽCD69 and the IL-2 receptor ŽCD25.. and to down- were harvested by centrifugation and washed with regulate TCR molecules on the responding T cell. sterile PBS Ž10 mM NaPO4 , 150 mM NaCl, pH 7.3. We also demonstrate that scFv-yeast can activate T before use.
  • B.K. Cho et al.r Journal of Immunological Methods 220 (1998) 179–188 181 2.3. Flow cytometric analysis of scFÕ-KJ16 expres- vested, washed in PBS that contained 0.1% BSA and sion 0.05% azide and stained with biotinylated clonotypic antibody, 1B2 ŽKranz et al., 1984.. After washing, Approximately 10 6 yeast cells Žone ml at one bound 1B2 and activation markers were detected OD600 ; 10 7 cells. displaying either scFv-KJ16 or with a mixture of streptavidin: PE and FITC Aga2-hemagglutinin ŽAGA-HA. were incubated with labeled-anti-CD69 or FITC labeled-anti-CD25 ŽIL- a mouse anti-c-myc IgG antibody Ž1r100 dilution of 2R. antibody. The yeast: T cell mixture was ana- 9E10 ascites. and scTCR–biotin conjugate at ; 1.5 lyzed by flow cytometry, gating on 1B2-positive T mM ŽSchodin et al., 1996. for 30 min on ice. The cells that were viable, based on exclusion of the vital scTCR contained a Vb8 domain that is recognized dye 7-aminoactinomycin D Ži.e. PEq 7-AADy .. For by the KJ16 antibody. Cells were washed, pelleted experiments monitoring the levels of surface TCR, and resuspended in media containing fluorescein- biotinylated 1B2 antibody was detected with strepta- labeled ŽFITC. goat anti-mouse IgG and streptavidin: vidin: PE and the yeast: T cell mixture was analyzed phycoerythrin ŽPE. as secondary reagents. Yeast were by flow cytometry gating on T cells by light scatter analyzed on a Coulter Epics XL flow cytometer at and cells that were 7AADy. the Flow Cytometry Center of the University of Illinois Biotechnology Center. From the fluorescence intensities observed using anti-HA and anti-c-myc 3. Results and discussion antibodies for scFv-KJ16-yeast in comparison with other scFv-yeast systems where more quantitative 3.1. Induction and characterization of scFÕ-KJ16 on assays were used ŽBoder and Wittrup, 1997; Kieke et the surface of yeast al., 1998; and unpublished results., we estimated that approximately 50,000 scFv-KJ16 molecules are ex- To explore the use of yeast cell surface display in pressed per yeast cell. T cell activation, a scFv was expressed as a fusion protein in which the scFv was linked to the C- 2.4. Preparation of KJ16 coupled latex microspheres terminus of Aga2p, an a mating type agglutinin protein that binds to the corresponding a agglutinin KJ16 IgG, purified as described by Cho et al. on the surface of a cells during yeast mating ŽBoder Ž1995 ., was covalently coupled to 5 m m and Wittrup, 1997.. Since the agglutinins’ native carboxylate-modified latex microspheres ŽBangs function involves external protein–protein recogni- Laboratories, Indiana. using a two-step coupling pro- tion, the Aga2p molecules are positioned at the cell cedure involving pre-activation with 1-Ž3-dimethyl- surface to allow for normal cell to cell interactions aminopropyl.-3-ethylcarbodiimide, as recommended ŽLu et al., 1995.. The scFv derived from the anti-Vb8 by the manufacturer. KJ16 IgG was coupled in 0.1 antibody KJ16 binds to the TCR of a cytotoxic T M borate buffer, pH 8.5 at a concentration of 1 lymphocyte ŽCTL. clone called 2C ŽRoehm et al., mgrml at room temperature overnight. Ethanolamine 1985; Cho et al., 1995.. The KJ16 scFv gene was Ž5 mM. was used to mask uncoupled sites and cloned and linked to the 3X end of the Aga2 gene. A microspheres were washed with sterile PBS prior to c-myc epitope tag was included at the C-terminus of use in culture. As a control, an irrelevant monoclonal the Aga2rKJ16 scFv fusion, to allow surface levels IgG Ž800E6. was coupled following the same condi- of scFv to be quantitated Žwith anti-c-myc antibody. tions. independent of antigen binding ability. Aga2–scFv fusion expression is induced by growth in galactose. 2.5. ActiÕation assays The scFv-yeast cells were examined by flow cy- tometry to determine if the protein was expressed on Splenocytes Ž10 5 . from 2C TCRrRAG-1y_y mice the cell surface and if so, whether they bound to were incubated at 378C, 5% CO 2 with various num- TCR molecules. To detect the yeast surface protein, bers of yeast cells that bear either scFv-KJ16 or a mouse antibody to the c-myc epitope was used AGA-HA. After 20 h in culture, cells were har- together with a FITC-labeled secondary anti-mouse
  • 182 B.K. Cho et al.r Journal of Immunological Methods 220 (1998) 179–188 IgG. To detect active scFv, the scFv-yeast cells were bated overnight with naive Vb8q T cells from 2C incubated with biotinylated-soluble TCR containing TCRrRAG-1yry transgenic mice ŽManning et al., a Vb8 domain ŽSchodin et al., 1996., followed by 1997. ŽFig. 2A.. The fraction of T cells that express phycoerythrin–streptavidin. Results were compared upregulated CD69 is dependent on the ratio of with a negative control yeast strain that expressed KJ16rmicrospheres to T cells, whereas control mi- only Aga2p ŽAGA-HA, Fig. 1. and yeast that ex- crospheres that contain an irrelevant IgG fail to press an scFv domain from an antibody of unrelated activate cells even at ratios that are 100 times those specificity Ždata not shown.. The scFv-yeast express- of the specific microspheres ŽFig. 2A.. ing a functional antibody domain on their surface Previous studies of scFv displayed on yeast have bound both the anti-c-myc antibody and soluble shown that as many as 10 5 fusion protein molecules Vb8q TCR. Binding of the soluble TCR was com- are expressed per cell ŽBoder and Wittrup, 1997., pletely inhibited by excess intact KJ16 antibody resulting in an antigen density considerably higher Ždata not shown.. than the density of peptiderMHC complexes re- quired for stimulating cytolytic activity ŽSykulev et 3.2. ActiÕation of 2C TCR r RAG1-1yry splenocytes al., 1996.. Furthermore, the average diameter of with scFÕ-KJ16 yeast yeast Ž4–5 mm. is the optimal cell size for stimulat- ing CTL responses ŽMescher, 1992.. To determine if Soluble anti-Vb8 antibody ŽKJ16. or the scFv surface expressed scFv could activate naive T cells, derived from this antibody are unable to induce T scFv-yeast or control yeast that only express the cell activation ŽRojo and Janeway, 1988, and data Aga2 protein were incubated at various cell numbers not shown.. However, like other anti-TCR antibodies with naive Vb8q T cells from the 2C TCRrRAG- Že.g. Deeths and Mescher, 1997., KJ16 IgG pre- 1yry transgenic mice. After co-incubation of yeast sented in multivalent form on five micron latex and resting T cells for 20 h, T cells were analyzed microspheres can induce signals that result in T cell for the induction of the activation markers CD69 and activation and upregulation of CD69, when incu- CD25 by flow cytometry. The clonotypic anti-TCR Fig. 1. Yeast that display the Aga2rscFv-KJ16rc-myc fusion on their surface bind biotinylated-Vb8 scTCR. Yeast transfected with Aga2rscFv-KJ16 or Aga2-hemagglutinin ŽAGA-HA. were incubated with anti-c-myc antibody Ž1r100 dilution of 9E10 ascites. and Vb8q scTCR–biotin Ž; 1.5 mM. for 30 min on ice. Cells were washed, pelleted and resuspended in media containing FITC-labeled goat anti-mouse IgG and streptavidin: PE as secondary reagents. Yeast were analyzed on a Coulter Epics XL flow cytometer at the Flow Cytometry Center of the University of Illinois Biotechnology Center.
  • B.K. Cho et al.r Journal of Immunological Methods 220 (1998) 179–188 183 Fig. 2. Induction of T cell activation markers on naive splenocytes in the presence of KJ16rmicrospheres and scFv-yeast. Splenocytes Ž10 5 . from 2C TCRrRAG-1y_y mice were cultured with ŽA. antibody-coupled microspheres or ŽB, C. yeast cells that bear either KJ16 scFv or Aga2-hemagglutinin ŽAGA-HA. for 20 h at 378C, 5% CO 2 . Cells were harvested, washed, and stained with biotinylated clonotypic antibody, 1B2. After washing, bound 1B2 and activation markers were detected with a mixture of streptavidin: PE and FITC labeled-anti-CD69 ŽA,B. or FITC labeled-anti-CD25 ŽIL-2R. antibody ŽC.. The yeast: T cell mixture was analyzed by flow cytometry, gating on 1B2-positive T cells that were viable, based on exclusion of the vital dye 7-aminoactinomycin D Ži.e. PEq 7-AADy .. For CD25 upregulation ŽC., results using a yeast to T cell ratio of 1:1 are shown. The percent of cells that were judged to be positive for CD69 Ži.e. within the area of the cursor bar. are indicated in each panel.
  • 184 B.K. Cho et al.r Journal of Immunological Methods 220 (1998) 179–188 antibody 1B2 was used in double staining to analyze able to completely inhibit the induction of the activa- only 2C Vb8q T cells ŽKranz et al., 1984.. Both tion markers ŽFig. 3.. CD69 ŽFig. 2B. and CD25 ŽFig. 2C. were upregu- Another process that occurs during TCR-induced lated on T cells exposed to the scFv-yeast. The activation of T cells is the down-regulation of sur- amount of upregulation correlated with the number face TCR after serial triggering with ligands of yeast used as activators and could be detected at Žpeptide–MHC complexes. of the appropriate affin- yeast to T cell ratios as low as 0.1 to 1 ŽFig. 2B., ity ŽValitutti et al., 1995.. The clonotypic anti-TCR similar to antibody-coupled microspheres ŽFig. 2A.. antibody, 1B2, and the anti-Vb8 antibody, KJ16, This ratio is considerably lower than the 1:1 ratio bind to different and non-overlapping epitopes on the typically used when transfected insect cells have TCR of 2C T cells ŽSchodin et al., 1996.. Therefore, served as ‘antigen presenting cells’ ŽCai et al., 1996; the 1B2 antibody could be used to evaluate changes Sun et al., 1996., even though the binding affinity of in the levels of TCR molecules on 2C T cells that the KJ16 antibody Ž K D ; 120 nM; Cho et al., 1995. interacted with the scFv-yeast. As shown in Fig. 4, is nearly the same as the peptiderMHC ligand, yeast that express cell surface scFv-KJ16 induced the QL9rLd Ž K D ; 100 nM; Sykulev et al., 1994. used in the latter studies. The lack of T cell activation in the presence of the control yeast ŽFig. 2B. indicated that yeast, like insect cells ŽCai et al., 1996; Sun et al., 1996., provide a relatively inert surface from which molecules important for eliciting a specific T cell response can be characterized. However, unlike in- sect cells, yeast are compatible with the optimal temperature for mammalian cell culture Ž378C.. This feature is particularly important for studies with im- mune cells, as incubation of insect cells at 378C leads to cell lysis and release of DNA that can result in activation and thus may complicate the analyses ŽCai et al., 1996; Sun et al., 1996.. For instance, previous studies showed that DNA derived from bacteria, yeast, and insect cells can induce spleen cell activation, as determined by proliferation assays ŽSun et al., 1996; Pisetsky, 1996.. Similarly, bacte- rial display systems that have been developed for combinatorial library screening ŽGeorgiou et al., 1997. are also likely to trigger nonspecific inflamma- tory responses. In addition, the lipopolysaccharide layer could introduce a steric barrier that would inhibit cell to cell contacts. In the present study, three lines of evidence demonstrate that the T cell activation induced by the scFv-yeast does not involve DNA-mediated stimula- tion. First, control yeast that do not bear the appro- Fig. 3. KJ16 Fab fragments inhibit T cell activation by scFv-yeast. priate scFv do not induce T cell activation markers Splenocytes Ž10 5 . from 2C TCRrRAG-1y_y mice were incu- ŽFig. 2B.. Second, the T cell population used here is bated at a 1:1 ratio with scFv-yeast in the presence or absence of 10 mgrml KJ16 Fab fragments or in the presence of a three-fold from a TCRrRAGyry transgenic mouse that contain excess of AGA-HA yeast Žtop panel.. After 20 h, the cell mixtures no B cells which are required in the DNA-induced were harvested and analyzed for CD69 induction as described in response. Finally, soluble KJ16 Fab fragments are Fig. 2.
  • B.K. Cho et al.r Journal of Immunological Methods 220 (1998) 179–188 185 3.3. ActiÕation of 2C TCR r RAG1-1yry splenocytes in the presence of irreleÕant yeast The ability to use the yeast display system to identify scFv or novel polypeptide ligands that act as agonists or antagonists depends ultimately on the ability to screen yeast libraries for a yeast cell that bears a specifically reactive polypeptide among many cells that bear different Žirrelevant. polypeptides. To find out if yeast meet this requirement, T cells were incubated with scFv-ŽKJ16. yeast in the presence or absence of various excesses of yeast expressing only the Aga2 polypeptide. After 20 h of incubation, CD69 levels were measured by flow cytometry, gat- ing on 1B2-positive Ž2C. T cells. All mixtures, in- cluding the highest ratio of nonspecific yeast to scFv-yeast tested Že.g. 30:1. induced levels of CD69 that were comparable to those induced by scFv-yeast alone ŽFig. 5.. Similar results were obtained when CD25 upregulation was monitored Ždata not shown. indicating that specific cell activation could occur even in the presence of a great excess of irrelevant yeast. Therefore, by using high-throughput screening assays in 96-well plate formats it should be possible to screen libraries in the range of 10 5 to 10 6 inde- pendent clones for yeast that express candidate poly- peptides capable of inducing such cellular responses. Over the past several years, many screening pro- cedures have been developed to identify new ligands that bind to a given receptor. However, procedures that are based solely upon binding are likely to lack Fig. 4. Downregulation of the TCR on T cells induced by scFv- yeast. scFv-yeast were incubated with 10 5 splenocytes from 2C the sensitivity of other procedures that exploit the TCRrRAG-1y_y mice at several different ratios of yeast to T great amplification that results from transducing sur- cells. After 20 h the cells were harvested and incubated with face receptor binding into cellular responses. For biotinylated-1B2 IgG followed by streptavidin: PE. TCR expres- instance, phage display typically selects for ligands sion was monitored by flow cytometry gating on T cells by light that bind to receptors in the mM to nM range. In scatter and cells that were 7AADy. The population at the lower PE levels Žlocated outside the region of the cursor bar. are yeast contrast, cellular responses to appropriate ligation of cells that were not entirely excluded by the light scatter criteria. surface receptors by natural ligands can often be Mean fluorescence units Žmfu. of the region within the cursor bar detected at pM concentrations or lower. However, are indicated. the triggering of such receptors often requires dimer- ization or oligomerization to induce activity Žre- viewed by Heldin, 1995.. Many display systems lack loss of TCR molecules from the 2C T cell surface, the ligand density required for activating cellular whereas control yeast without surface scFv were responses, and therefore cannot take advantage of the unable to induce this downregulation. These results signal amplification inherent in these processes. Ac- are consistent with recent findings that TCR down- cordingly, previous reports that have screened for regulation can occur in the absence of costimulation agonist molecules that induced receptor dimerization ŽViola and Lanzavecchia, 1996.. have had to rely on multiple steps of which the first
  • 186 B.K. Cho et al.r Journal of Immunological Methods 220 (1998) 179–188 is direct binding ŽWrighton et al., 1997; Xie et al., of interest, and triggers cellular responses. These 1997.. Because binding assays lack the sensitivity of findings suggest that the yeast Aga2-fusion system cellular response assays, novel ligands may not be has the potential for generating and screening pep- detected. tide or cDNA libraries for bioactive molecules. For In this report, we describe a yeast display system example, thymus-specific cDNA libraries might be that expresses a high density of cell surface fusion screened for novel cell surface ligands that are in- proteins, crosslinks target receptors present on cells volved in particular T cell developmental functions ŽCibotti et al., 1997. or covalently linked peptide– MHC complexes ŽKozono et al., 1994. might be screened as T cell agonists. It is also reasonable to suggest that in cases where ligation of multiple different receptors are required, that it will be possi- ble to co-express different ligands using multiple selectable markers on the appropriate Aga2-yeast vectors. Although our results emphasize the utility of this system as it applies to T cells, a similar approach may be used to generate agonist or antagonist molecules towards a wide variety of other cell sur- face receptors that likewise induce cellular responses upon crosslinking ŽHeldin, 1995.. It is important to note that yeast display is signifi- cantly different from presentation of proteins on the surface of inert latex or silica microspheres. For instance, yeast display avoids the need to clone, express, refold in vitro, andror purify the gene productŽs. to be tested. Indeed, the inherent attrac- tion for this system is its potential for screening the biological activity of polypeptides derived from, as of yet, unidentified genes or even from uncharacter- ized open-reading frames. For these purposes, cur- rent techniques provide yeast transformation effi- ciencies that are on the order of bacterial transforma- tions with library sizes of up to 10 7 independent colonies ŽGietz et al., 1995 and unpublished data.. Furthermore, since yeast have a well-defined genetic system with many selectable markers, they have the Fig. 5. Yeast that bear scFv-KJ16 activate T cells in the presence of excess irrelevant yeast. Yeast that express only Aga2 ŽAGA-HA. were mixed with a constant number of scFv-ŽKJ16. yeast at several different ratios and the mixture was incubated with 10 5 splenocytes from 2C TCRrRAG-1y_y mice. After 20 h in culture, the levels of CD69 induction on T cells were determined by flow cytometry, as described in Fig. 2. Similar results have been obtained when T cells were analyzed for CD25. The control Žtop panel. indicates the level of CD69 expression on T cells when incubated only with irrelevant yeast, at the highest yeast to T cell ratio used.
  • B.K. Cho et al.r Journal of Immunological Methods 220 (1998) 179–188 187 potential for displaying several polypeptides simulta- Jorgensen, J.L., Reay, P.A., Ehrich, E.W., Davis, M.M., 1992. neously on the same yeast cell allowing for the study Molecular components of T-cell recognition. Annu. Rev. Im- munol. 10, 835. or even selection of co-receptor molecules. Kieke, M.C., Cho, B.K., Boder, E.T., Kranz, D.M., Wittrup, K.D., 1998. Isolation of anti-T cell receptor scFv mutants by yeast surface display. Protein Eng. 10, 1303. Acknowledgements Kozono, H., White, J., Clements, J., Marrack, P., Kappler, J., 1994. Production of soluble MHC class II proteins with cova- lently bound single peptides. Nature 369, 151. We thank Drs. Kappler and Marrack for originally Kranz, D.M., Tonegawa, S., Eisen, H.N., 1984. Attachment of an providing the KJ16 hybridoma line and Gary Durack anti-receptor antibody to non-target cells renders them suscep- and the University of Illinois Flow Cytometry Facil- tible to lysis by a clone of cytotoxic T lymphocytes. Proc. Natl. Acad. Sci. USA 81, 7922. ity for assistance with flow cytometry. We also thank Lu, C.F., Montijn, R.C., Brown, J.L., Klis, F., Kurjan, J., Bussey, Dr. Herman Eisen for helpful comments on the H., Lipke, P.N., 1995. Glycosyl phosphatidylinositol-depen- manuscript. This work was partially supported by dent cross-linking of alpha-agglutinin and beta 1,6-glucan in grants from the Whitaker Biomedical Engineering the Saccharomyces cereÕisiae cell wall. J. Cell Biol. 128, 330. Foundation Žto KDW. and the NIH ŽR01AI35990 to Manning, T.C., Rund, L.A., Gruber, M.M., Fallarino, F., Gajew- ski, T.F., Kranz, D.M., 1997. Antigen recognition and allo- DMK.. E. Boder was supported by an NSF Graduate genic tumor rejection in CD8qTCR transgenicrRAGyry Fellowship. M. Kieke was supported as a trainee on mice. J. Immunol. 159, 4665. NIH T32 GM07283. Mescher, M.F., 1992. Surface contact requirements for activation of cytoxic T lymphocytes. J. Immunol. 149, 2402. Metzger, H., 1992. Transmembrane signaling: the joy of aggrega- tion. J. Immunol. 149, 1477. References Petsko, G., 1996. For medicinal purposes. Nature 384, 7, ŽSupple- ment.. Allison, J.P., Krummel, M.F., 1995. The yin and yang of T cell Pisetsky, D.S., 1996. The immunologic properties of DNA. J. costimulation. Science 270, 932. Immunol. 156, 421. Boder, E.T., Wittrup, K.D., 1997. Yeast surface display for Reich, Z., Boniface, J.J., Lyons, D.S., Borochov, N., Wachtel, screening combinatorial polypeptide libraries. Nat. Biotech. E.J., Davis, M.M., 1997. Ligand-specific oligomerization of 15, 553. T-cell molecules. Nature 387, 617. Cai, Z., Brunmark, A., Jackson, M.R., Loh, D., Peterson, P.A., Roehm, N.W., Carbone, A., Kushnir, E., Taylor, B.A., Riblet, Sprent, J., 1996. Transfected drosophila cells as a probe for R.J., Marrack, P., Kappler, J.W., 1985. The major histocom- defining the minimal requirements for stimulating unprimed patibility complex-restricted antigen receptor on T cells: the CD8q T cells. Proc. Natl. Acad. Sci. USA 93, 14736. genetics of expression of an allotype. J. Immunol. 135, 2176. Cho, B.K., Schodin, B.A., Kranz, D.M., 1995. Characterization of Rojo, J.M., Janeway, C.A. Jr., 1988. The biologic activity of a single-chain antibody to the b-chain of the T cell receptor. J. anti-T cell receptor V region monoclonal antibodies is deter- Biol. Chem. 270, 25819. mined by the epitope recognized. J. Immunol. 140, 1081. Cibotti, R., Punt, J.A., Dash, K.S., Sharrow, S.O., Singer, A., Scharenberg, A.M., Kinet, J.P., 1996. The emerging field of 1997. Surface molecules that drive T cell development in vitro receptor-mediated inhibitory signaling: SHP or SHIP?. Cell in the absence of thymic epithelium and in the absence of 87, 961. lineage-specific signals. Immunity 6, 245. Schodin, B.A., Schlueter, C.J., Kranz, D.M., 1996. Binding prop- Deeths, M.J., Mescher, M.F., 1997. B7-1-dependent co-stimula- erties and solubility of single-chain T cell receptors expressed tion results in qualitatively and quantitatively different re- in E. coli. Mol. Immunol. 33, 819. sponses by CD4q and CD8q T cells. Eur. J. Immunol. 27, Singer, S.J., 1992. Intercellular communication and cell–cell ad- 598. hesion. Science 255, 1671. Georgiou, G., Statopoulos, C., Daugherty, P.S., Nayak, A.R., Sun, S., Cai, Z., Langlade-Demoyen, P., Kosaka, H., Brunmark, Iverson, B.L., Curtiss, R., 1997. Display of heterologous A., Jackson, M.R., Peterson, P.A., Sprent, J., 1996. Dual proteins on the surface of microorganisms: from the screening function of drosophila cells as APCs for naive CD8qT cells: of combinatorial libraries to live recombinant vaccines. Nat. implications for tumor immunotherapy. Immunity 4, 555. Biotech. 15, 29. Sykulev, Y., Brunmark, A., Tsomides, T.J., Kageyama, S., Jack- Gietz, R.D., Schiestl, R.H., Willems, A.R., Woods, R.A., 1995. son, M., Peterson, P.A., Eisen, H.N., 1994. High-affinity Studies on the transformation of intact yeast cells by the reactions between antigen-specific T-cell receptors and pep- LiAcrSS-DNArPEG procedure. Yeast 11, 355. tides associated with allogenic and syngeneic major histocom- Heldin, C.H., 1995. Dimerization of cell surface receptors in patibility complex class I proteins. Proc. Natl. Acad. Sci. USA signal transduction. Cell 80, 213. 91, 11487.
  • 188 B.K. Cho et al.r Journal of Immunological Methods 220 (1998) 179–188 Sykulev, Y., Joo, M., Vturina, I., Tsomides, T.J., Eisen, H.N., Wrighton, N.C., Balasubramanian, P., Barbone, F.P., Kashyap, 1996. Evidence that a single peptide–MHC complex on a A.K., Farrel, F.X., Jolliffe, L.K., Barrett, R.W., Dower, W.J., target cell can elicit a cytolytic T cell response. Immunity 4, 1997. Increased potency of an erythropoietin peptide mimetic 565. through covalent dimerization. Nat. Biotechnol. 15, 1261. Valitutti, S., Muller, S., Cella, M., Padovan, E., Lanzavecchia, A., Xie, M.H., Yuan, J., Adams, C., Gurney, A., 1997. Direct demon- 1995. Serial triggering of many T-cell receptors by a few stration of MuSK involvement in acetylcholine receptor clus- peptide–MHC complexes. Nature 375, 148. tering through identification of agonist scFv. Nat. Biotech. 15, Viola, A., Lanzavecchia, A., 1996. T cell activation determined by 768. T cell receptor number and tunable thresholds. Science 273, Zaccolo, M., Griffiths, A.P., Prospero, T.D., Winter, G., Gherardi, 104. E., 1997. Dimerization of Fab fragments enables ready screen- Winter, G., Griffiths, A.D., Hawkins, R.E., Hoogenboom, H.R., ing of phage antibodies that affect hepatocyte growth 1994. Making antibodies by phage display technology. Annu. factorrscatter factor activity on target cells. Eur. J. Immunol. Rev. Immunol. 12, 433. 27, 618.