Mechanisms of Microbial Pathogenicity


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Mechanisms of Microbial Pathogenicity

  1. 1. Universitat de les UIB Illes Balears Master in Microbiology COURSE DESCRIPTION 2006-2007 Academic Year Technical information Course Course title: Mechanisms of Microbial Pathogenicity Course code: a cumplimentar por el Centro de Tecnologías de la Información Type of course: Optional Level of course: Postgraduate Year of study: First Semester: Second Timetable: See the timetable for the Master in Microbiology programme Language of instruction: Spanish/Catalan, reading comprehension skills in English required Lecturers Supervising lecturer Name: Dr. Sebastián Albertí Contact: Other lecturers Name: Contact: Name: Contact: Name: Contact: Name: Contact: Prerequisites: A solid background (at the bachelor’s degree level) in biochemistry, molecular biology, genetics, cellular biology, microbiology, plant-animal physiology and chemistry Number of ECTS credits 5 Number of classroom hours: 40 Independent study hours: 85 Description: Integrated immune system responses to infectious agents. Therapeutic applications. Course competences Specific: E2- Become familiar with and adept at handling various immunological techniques E4- Apply methodological procedures to the study of antimicrobials E5- Grasp the strategies (pathogenicity mechanisms) used by microorganisms to escape the immune system and cause infection and the therapeutic applications involved Generic: G1- Acquire an integrated view of microorganisms, their biological properties and their roles and applications in the fields of ecology, health, industry, agriculture and biotechnology
  2. 2. G2- To enhance prior knowledge of microbiology and the elements involved in developing and/or applying ideas at the research level as well as in other areas Course contents THEORETICAL CONTENT 1. Bacteria-host interactions. Types of interactions. Normal microbiota. Parasite interactions: the concepts of pathogenicity and virulence. Stages in infectious processes. 2. Epidemiology of infectious disease. Principles and methods. Transmission and control. Principles of chemoprophylaxis and immunoprophylaxis 3. Immunity to viruses 4. Immunity to bacteria 5. Immunity to fungi 6. Factors in microbial virulence. Identification of virulence factors. Adhesins. Invasins. Toxins. Immune system resistance factors. Other factors in virulence. 7. Infectious diseases, their etiologic agents and virulence factors: Gram-positive microorganisms (Staphylococcus aureus, Staphylococcus epidermidis and other coagulase-negatives, Streptococcus Beta-haemolytic, Streptococcus pneumoniae, Listeria monocytogenes and Bacillus anthracis) 8. Infectious diseases, their etiologic agents and virulence factors: Gram-negative microorganisms (Neisseria meningitidis, Neisseria gonorrhoeae, Vibrio cholerae, Enterobacteria, Pseudomonas aeruginosa, Haemophilus influenzae, Brucella, Legionella pneumophila) 9. Infectious diseases, their etiologic agents and virulence factors: Other microorganisms (Clostridium, Bacteroides, Mycobacterium tuberculosis, Fungi) 10. Main clinical systems and their correlation with virulence factors PRACTICAL CONTENT 1. In vitro study of innate and specific immune effect mechanisms. Bacterial factors of resistance. The complement system. Opsonization. Antimicrobial peptides. Phagocytosis. The effect of antibodies. 2. Integrated study of the immune system response against bacterial infection. Animal models in studying infections. Methodology and student workload 1. Teaching method: Classroom sessions Classroom/independent work: 10/50 E-learning: yes Type of group: whole group 2. Teaching method: Laboratory Classroom/independent work: 25/10 E-learning: yes Type of group: pairs 3. Teaching method: Tutorials Classroom/independent work: 2/0 E-learning: no Type of group: groups of four 4. Teaching method: Theoretical study Classroom/independent work: 0/25 E-learning: yes
  3. 3. Type of group: individual 5. Teaching method: Evaluation Classroom/independent work: 3/0 E-learning: no Type of group: individual Assessment instruments, criteria and learning agreement Assessment criteria: Knowledge of microorganism strategies (pathogenicity mechanisms) to escape the immune system. Familiar with the different methodologies involved in studying antimicrobials and skill at handling various immunological techniques. Students must attend practical sessions and pass the final exam to earn a passing grade in the course. Assessment instruments: A written exam on the course’s theoretical and practical content Laboratory notebook Lecture classes Grading criteria A written exam on theoretical and practical course content: 75% On-going assessment of laboratory notebook: 20% On-going assessment of lecture classes: 5% Assessment based on a learning agreement: No Independent study material and recommended reading Bibliography, resources and annexes BOOKS ABBAS, K.A., POBER, J.S. and LICHTMAN, AH. 2003. Cellular and molecular immunology. McGrawHill. New York. AYOUB, E.M., CASSELL, G.H., BRANCHE, W.C. and HENRY, T.J. 1990. Microbial Determination of Virulence and Host Response. ASM. Washington. DAVIS, B.D., DULBECCO, R., EISEN, H.N. and GINSBERG, H.S. 1997. Tratado de Microbiology. Salvat SA. Barcelona. DORMAN, C.J. 1994. Genetics of bacterial virulence. Blackwell Scientific Publications. London. EVANS, A.S. and FELDMAN, H. 1991. Bacterial Infections of Humans. Epidemiology and Control. Plenium Press. London. ISENBERG, H.D. 2004. Clinical microbiology. Procedures Handbook. ASM. Washington. KREIER, J.P. and MORTENSEN, R.F. 2001. Infection, Resistance and Immunity. ASM. Washington. MANDELL, G.L., BENNETT, J.E. and DOLIN, R. 2000. Principles and practice of infectious diseases. Churchill-Livingstone. NW. MIMS, C.A., PLAYFAIR, J.H.L., ROITT, I.M., WAKELIN, D. and WILLIAMS, R. 1993. Vaccination. Mosby. London. MISS, C., IMMOCK, N., NASH, A. and STEPHEN, J. 1995. (4ª ed). Mims' Pathogenesis of Infectious Diseases. Academic Press. London. MURRAY, P.R. 2002. Microbiology médica. Mosby España. Madrid. PUMAROLA, A., RODRÍGUEZ TORRES, A., GARCÍA RODRÍGUEZ, J.A. y PIEDROLA ANGULO, G. 1999. Microbiology y parasitología médicas. Masson. Barcelona.
  4. 4. ROITT, I.M. 2001. Essential Immunology. Blackwell scientific. Oxford. ROTH, J.A. 1995. Virulence Mechanisms of Bacterial Pathogens. ASM. Washington. SALYERS, A. A. andWHITT, D.D. 2001. Bacterial pathogenesis. A molecular approach. ASM. Washington. RYAN, K.J.and RAY, C.G. 2004. Sherris medical microbiology. An introduction to infectious diseases. Elsevier. New York. SUSSMAN, M. 1997. Escherichia coli: Mechanisms of virulence. Cambridge University Press. Cambridge. TORTORA, G.J., FUNKE, B.R. and CASE, C.L. 2004. Microbiology: An introduction. Pearson Education Inc. Benjamin Cummings. Menlo Park. CA. JOURNALS (available on-line at the UIB library) DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASES. Elsevier Science Publishers. New York. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTIOUS DISEASES. Vieweg Sohn Verlag. Wiesbaden. INFECTION AND IMMUNITY. American Society for Microbiology. Washington. INVESTIGACION Y CIENCIA. Prensa Científica, S.A. Barcelona. JOURNAL OF CLINICAL MICROBIOLOGY. American Society for Microbiology. Washington. JOURNAL OF INFECTIOUS DISEASES. University Chicago Press. Chicago. JOURNAL OF MEDICAL MICROBIOLOGY. Churchill Livingstone. Edinburgh. LA RECHERCHE. Societé des Éditions Scientifiques. Paris. MOLECULAR MICROBIOLOGY. Blackwell Scientific Publications. Oxford. MUNDO CIENTIFICO.Ed.Fontalba, S.A. Barcelona. NATURE. McMillan Co., Hampshire. SCIENCE. American Association for the Advancement of Science, Washington. SCIENTIFIC AMERICAN. Science American Inc., New York. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES.National Academy of Sciences of USA. Washington. Link to the course teaching guide