Jiahuai Wang, Associate Professor,


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Jiahuai Wang, Associate Professor,

  1. 1. prepared 2/1/2009 CURRICULUM VITAE Name: Jia-huai Wang Address: 81 Alexander Avenue, Belmont, MA 02478 Place of birth: Shanghai, China Date of birth: March 19, 1941 Education: 1963 B.S. University of Science & Technology of China, Beijing, China 1979 Ph.D. Institute of Biophysics, Chinese Academy of Sciences, Beijing Post-doctoral Training: 1979-1981 Visiting Scholar, Department of Biochemistry, University of Wisconsin, Madison, WI 1981-1982 Visiting Scholar, Department of Biochemistry & Molecular Biology, Harvard University, Cambridge, MA Academic Appointments: 1979-1986 Assistant Professor, Institute of Biophysics, Chinese Academy of Sciences 1986-1988 Associate Professor, Institute of Biophysics, Chinese Academy of Sciences 1988-1990 Professor, Institute of Biophysics, Chinese Academy of Sciences 1990-1995 Research Associate, Department of Biochemistry & Molecular Biology, Harvard University, Cambridge, MA 1996-2001 Principal Scientific Associate in Medicine, Laboratory of Immunobiology and X-ray Crystallography Unit, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 1996-2001 Lecturer in Pediatrics, Children's Hospital, Harvard Medical School 2001- Associate Professor of Pediatrics (BCMP), Harvard Medical School 2001- Associate Professor, Dana-Farber Cancer Institute Institute Appointments: 1984-1987 Vice-director, Department of Protein Crystallography, Institute of Biophysics, Chinese Academy of Sciences 1987-1990 Director, Department of Protein Crystallography, Institute of Biophysics, Chinese Academy of Sciences
  2. 2. Awards and Honors: 1982 Natural Science Award of China 1987 Outstanding Scientist, awarded by State Council of China Editorial Boards: 1997- Member on the editorial board for “Macromolecular Structure Pre-90” Chief editor: Wayne Hendrickson, asssociate editors: Jan Drenth and Kurt Wuthrich, Publisher: Current Biology Ltd., London, UK. Memberships in Professional Societies: 1978-1991 Chinese Biophysics Association 1990- American Association for the Advancement of Science 1993- Society of Chinese Bioscientists in America 1993-2000 New York Academy of Sciences 1997- Ray Wu society, USA 2007- American Crystallographic Association Committee Assignments: 1987-1990 National 863 Committee of Biotechnology, China 1987-1990 Committee of Biotechnology, Chinese Academy of Sciences 1997 Allergy, Immunology, and Transplantation Research Committee of NIAID 2005-2010 Scientific Advisory Committee, Structural Biology Center, Argonne National Laboratories 2007.12 Ad Hoc member of NIAID’s Board of Scientific Counselors Major Research Interests: 1. Structure, function and evolution of immunoglobulin superfamily proteins from immune and neuronal systems. 2. Structure and function of other cell adhesion receptors. 3. Structural studies of viral proteins of HIV and flu and drug design 4. Structural studies on virus receptors and their complexes with viral surface proteins Teaching Experience: 1982-1988 Lectures on "Principles of protein structures", course in Molecular Biology Chinese Academy of Sciences Graduate School, 10 lectures per year, China 2003−2007 BCMP201: “Proteins: Structure, Function and catalysis”, Harvard Medical School, BBS graduate course, Discussion leader.
  3. 3. List of former trainees who become principal investigators: 1983.1985Master degree trainee: Zi-he Rao, now the President of Nankai University, China and a professor in the School of Life Sciences, Tsinghua University, Beijing, China 1983.1986Master degree trainee: Wen-qing Xu, now a professor in University of Washington, School of Medicine. Seattle, WA 1985.1989 Post-master trainee: Xue-jung Zhang, now an associate professor in University Of Oklahoma, Medical Research Foundation, Oklahoma city, OK 1995-1998 Postdoctor trainee (co-adviser with Timothy Springer): Jose Casasvonas, now a principal investigator at Centro Nacional de Biotecnologia, CSIC, Campus Universidad Autonoma, Spain 2001-2006 Postdoctor trainee (co-adviser with Timothy Springer): Can Xiao, now a principal investigator at NIAID, NIH 2001-2006 Postdoctor trainee: Rob Meijers, now a principal investigator at PROXIMA II beamline at Soleil Saint Aubin, France 2004-2008 Postdoctor trainee: Hongmin Zhang, now an assistant professor at Hong Kong University, Hong Kong, China Lectures: 1986 "Trichosanthin: From Chinese herb medicine to ribosome inactivating protein", Oxford University, Lab. of Molecular Biophysics, Oxford, UK 1986 "Trichosanthin: ...", Birkbeck College, Dept. of Crystallography, London, UK 1986 "Trichosanthin:...", Imperial College, The Blacket Lab., London, UK 1986 "Trichosanthin: ...", Univ. of Gröningen, Dept. of Chemistry, Gröningen, The Netherlands 1987 "Three dimensional structure of protein and nucleic acid", Intl. Society of Biophysics summer course, Beijing, China 1988 "Trichosanthin: ...", Chinese Univ. of Hong Kong, Dept. of Biochemistry, Hong Kong 1988 "Trichosanthin: ...", Forum by International Center for Biotechnology, Trieste, Italy 1989 "Trichosanthin: ...", Univ. of Oregon, Inst. of Molecular Biology, Eugene, OR 1989 "Trichosanthin: ...", Max-Planck Inst. für Biochemie, Munich, Germany 1990 "Crystal structure of N-terminal two domain fragment of CD4 and its bio- logical implication", Purdue Univ, Dept. of Biological Sciences, W. Lafayette, IN 1990 "Crystal structure of N-terminal...", Ohio State Univ, Center for Biotech- nology, Columbus, OH 1991 "Crystal structure of N-terminal...", Boston Univ., Biophysics Program, Boston, MA 1991 "Crystal structure of N-terminal...", NIH, Bethesda, MD 1992 "Crystal structure of N-terminal...", Univ. of Pennsylvania, Dept. of Biochemistry & Biophysics, Philadelphia, PA 1993 "Structural studies of CD4: How it is subverted to be the receptor for HIV" Najing University, Nanjing, China 1993 "Structure, Function and Evolution of CD4", Conference of Chinese Bioscientists in America, Baltimore, MD
  4. 4. 1993 "Structure of HIV receptor-CD4", Intl. Congress of Crystallography, Beijing, China 1994 "Crystal structure of N-terminal...", Univ. of Virginia, Dept. of Molecular Physiology & Biological Physics 1995 "Crystal structure of N-terminal two domain fragment of VCAM-1: A cyclic peptide based on the domain 1 C-D loop can inhibit VCAM-1/ a4 interaction", 6th Intl. Symp. of Chinese Bioscientists in America, Vancouver, BC 1995 "Crystal structure of VCAM-1, the comparison to that of CD4", LeukoSite Inc., Boston, MA 1995 "Crystal structure of VCAM-1...", Dana-Farber Cancer Institute 1995 "Crystal structure of VCAM-1...", Center for Blood Research, Harvard Medical School, Boston, MA 1995 "Crystal structure of VCAM-1...", Fuji Immunopharmaceutical Inc., Boston, MA 1996 “The crystal structure of ICAM-2, its comparison to other integrin-binding proteins”. 17th Intl. Congress of Crystallography, Seattle, WA 1997 “The crystal structure of ICAM-2 reveals presentation of a distinctive integrin recognition surface”, Gordon Research Conferences, Ventura, CA 1997 “Structure of a complex of T-cell receptor and Fab”, 7th Intl. Symp. of Chinese Bioscientists in America, Toronto, Canada 1997 “Crystal structures of Ig superfamily proteins as integrin ligands” 37th American Society for Cell Biology Annual Meeting, Washington, DC 1999 “Structure of a heterophilic adhesion complex between the human CD2 and CD58 (LFA-3)” 18th Intl. Congress of Crystllography, Glasgow, UK 2000 “Structures of antigen specific and non-specific immune receptor complexes” Federation of American Society of Experimental Biology, Summer Research Conference 2000, Saxtons River, MT 2000 “Structural basis of immune recognition”, Lecture in plenary session of 15th conference of Federation of Asian and Oceanian Biochemistry and Molecular Biologists, Beijing, China 2001 “Protein recognition on cell surface”, The 1st Tsinghua International Conference on Protein Science, Beijing, China 2001 “Structural basis of cellular immunity”, The Fragrant Hill meeting on immunology, Beijing, China 2002 “What makes a virus receptor: A structural perspective”, The Novum Lecture, Karolinska Institute, Stockholm, Sweden 2003 Molecular mechanism of cell adhesion: Structural perspective”, McGill University Medical Center, Montreal, Canada 2003 “Molecular structure of ICAM-1 ecto-fragment and its implicated role in immunological synapse function”, Gordon Research Conferences, Ventura, CA 2004 “Atomic resolution views of the immunological synapse National Jewish Medical and Research Center and School Of Medicine, UCHSC, Denver, CO 2004 “Molecular mechanism of cell adhesion”, International symposium on mechanism of cell adhesion. Iksan, South Korea 2004 “Atomic resolution view of immunological synapse”, 10th International symposium of The Society of Chinese Bioscientists in America. Beijing, China 2005 “Molecular mechanism of cell-cell interaction in immune system”, 2005 FASEB Summer Research Conference: Immunoreceptors, Tucson, AZ 2006 “Molecular basis of cellular immunity”, Chinese University of Hong Kong 2006 “Structure and bi-functionality of Dscam headpiece: One stone for two birds”. Annual meeting of American Crystallography Association, July 06, Hawaii
  5. 5. 2006 Two lectures at the summer school on structural biology of China: 1) “Structural immunology”; 2) “Structural perspective of viral infection”, Hefei, China 2006 “Structure and bi-functionality of Dscam headpiece: One stone for two birds”. UCLA, Department of Biological Chemistry, Los Angeles, CA. 90095 2006 Lectures of “X-ray crystallography”, an informal mini-course at Dana-Farber for postdoctoral fellows, Boston, MA. 2007 “Structure of Dscam reveals its dual function in nervous and immune systems” University of Washington, Department of Biochemistry, Seattle, WA. 2007 “Structure and function of Dscam: One stone for two birds”. Harvard Medical School-Beijing Biophysics Institute Bilateral Conference on Structural Biology, 2007, Beijing, China (I am the co-organizer) 2007.10 “What determines the binding affinity of CD8—Structural perspective” International CD8 Group conference, Amherst, MA. 2007.11 “Virus/host cell interaction: From structure to medical implication”. School of Life Sciences, Beijing University, Beijing, and Fudan Medical School, Shanghai, China 2008.6. “Decoding recognition specificity of Dscam, a neural receptor with thousands isoforms”, MRC National Institute for Medical Research, London, UK. 2008.6 “Decoding recognition specificity of Dscam, a neural receptor with thousands isoforms”, Oxford University, UK. 2008.7 “Unique features of protein-protein interaction on cell surface”, The 2nd International Conference on Molecular Aspects on Protein-Protein Interaction, Dubrovnik, Croatia. 2008.8. “Decoding recognition specificity of Dscam, a neural receptor with thousands isoforms”, 21st International Congress of Crystallography, Osaka, Japan. 2009.1 “Protein-protein interaction on cell surface”, The Scripps Institute, Lo Jolla, CA.
  6. 6. Bibliography: Original reports: 1. Lin ZJ, Wang JH, Fan HF. Crystal structure of L-pro-Gly. Science Bulletin 1965; 2:148-150. 2. Beijing Insulin Structure Research Group. Studies on crystal structure of insulin at 2.5Å. Scientia Sinica (Chinese). 1972; 1:3-30. 3. Beijing Insulin Structure Research Group. Studies on the insulin crytsal structure: The molecule at 1.8Å resolution. Scientia Sinica. 1974; 17:752-778. 4. Wu BM, Wang JH. X-ray studies on two kinds of fibrous proteins from a cadaver preserved over 2000 years in a Han tomb. Scientia Sinica 1980; 23:915-922. 5. Rubin JR, Wang JH. Sundaralingam M: X-ray diffraction study of the zinc (II) binding sites in yeast phenylalanine transfer RNA. Biochi. et Biophs. Acta. 1983; 756:111-114. 6. Stuart D, Dai JB, Lou MZ, You JM, Todd R, Li JY, Wang JH, Liang DC. X-ray studies on the structure of des-pentapeptide insulin at 2.4Å resolution—The refinement of the structure. Scientia Sinica 1984; 27:1265-1272. 7. Stuart D, Dai JB, Lou MZ, You JM, Todd R, Li JY, Wang JH, Liang DC. X-ray studies on the structure of des-pentapeptide insulin at 2.4Å resolution.—Solution of the phase problem using the anomalous scattering of cadmium and the derivation of a model structure. Scientia Sinica 1984; 27:572-582. 8. Lewis M, Wang JH. Molecular polarity in DNA-protein recognition. Scientia Sinica 1985; B28:244-246. 9. Wang JH. Major groove or minor groove. Nature 1986; 319:183-184. 10. Zhang XJ, Wang JH. The sequence homology of trichosanthin and ricin A chain. Nature 1986; 321:477-478. 11. Wang JH. The major groove of B-DNA is the best binding site for the recognition helix of protein. J Biochem (China) 1986; 2:9-12. 12. Pan KZ et al. The three dimensional structure of trichosanthin molecule. Scientia Sinica 1987; B30:386-391. 13. Ma XQ, Wang YP, Wang JH. An X-ray analysis of the orthorhombic crystal form of trichosathin at 5A. Scientia Sinica 1987; B30:692-697. 14. Wang JH, Yan Y, Garrett T, Liu J, Rodgers D, Garlick RL, Tarr GE, Husain Y, Reinherz EL, Harrison SC. Atomic structure of a fragment of human CD4 containing two immunoglobulin-like domains. Nature 1990; 348:411-418.
  7. 7. 15. Feng Z, Li WW, Yeung HW, Chen SZ, Wang YP, Lin XY, Dong YC, Wang JH. Crystals of alpha-mormorcharin, a new ribosome-inactivating protein. J Mol Biol. 1990; 214:625-626. 16. Garrett TPJ, Wang JH, Yan YW, Liu JH, Harrison SC. Refinement and analysis of the structure of the first two domains of CD4. J Mol Biol. 1993; 234:763-778. 17. Wang JH, Perpinsky RB, Karpusas M, Lin JH, Osborn L. Crystallization and preliminary crystallographic studies on N-terminal two domain fragment of VCAM-1. Proteins: Struct. Funct. Genet. 1994; 20:287-290. 18. Wang JH, Perpinsky RB, Stehle T, Liu JH, Karpusas M, Browning B, Osborn L. Crystal structure of an N-terminal two domain fragment of VCAM-1: a cyclic peptide based on the domain 1 C-D loop can inhibit VCAM-1/ α4 integrin interaction. Proc Natl Acad Sci USA 1995; 92:5714-5718. 19. Karpusas M, Hsu YM, Wang JH, Thompson J, Lederman S, Chess L, Thomas D. 2Å crystal structure of an extracellular fragment of human CD40 ligand. Structure 1995; 3:1031-1039. 20. Wang JH, Stehle T, Pepinsky RB, Liu JH, Karpusas M, Osborn L. The crystal structure of a functional fragment of VCAM-1 refined at 1.9Å resolution. Acta Cryst. 1996; D52: 369-379. 21. Liu J,Tse AGD, Chang HC, Liu JH, Wang JH, Hussey RE, Chishti Y, Reinhold B, Spoerl R, Nathenson SG, Sacchettini JC, Reinherz EL. Crystallization of a deglycosylated T cell receptor (TCR) complexed with an anti-TCR Fab fragment. J Biol Chem. 1996; 271: 33639-33646. 22. Tan K, Liu J-H, Wang J-H, Shen S, Lu M. Atomic structure of a thermostable subdomain of HIV gp41. Proc. Natl. Acad. Sci. USA. 1997; 94:12303-12308. 23. Casasnovas JM, Springer TA, Liu JH, Harrison SC, Wang JH. The crystal structure of ICAM-2 reveals a distinctive integrin recognition site. Nature 1997; 387:312-315. 24. Wang J-H, Lim K, Smolyar A, Teng M-K, Liu J-H, Tse AGD, Liu J, Hussey RE, Chishti Y, Thomson CT, Sweet RM, Nathenson SG, Chang H-C, Sacchettini JC, Reinherz EL. Atomic structure of an αβ T cell receptor (TCR) heterodimer in complex with an anti- TCR Fab fragment derived from a mitogenic antibody. EMBO J. 1998; 271:278-293. 25. Teng M-K, Smolyar A, Tse AGD, Liu J-H, Liu J, Hussey RE, Nathenson SG, Chang H- C, Reinherz EL, Wang J-H. Crystal structure of the N15 TCR in complex with its peptide/MHC (pMHC) ligand: Identification of a common docking topology with substantial variation among different TCR-pMHC complexes. Curr Biol. 1998; 8:409-412. 26. Casasnovas JM, Stehle T, Liu JH, Wang JH, Springer TA. A dimeric crystal structure for the N-terminal two domains of ICAM-1. Proc. Natl. Acad. Sci. USA. 1998; 95:4134-39
  8. 8. 27. Tan K, Casasnovas JM, Liu J-H, Briskin MJ, Springer TA, Wang J-H. The structure of immunoglobulin superfamily domains 1 and 2 of MAdCAM-1 reveals novel features important for integrin recognition. Structure. 1998; 6:793-801. 28. Ghendler Y, Teng M-K, Liu J-H, Witte T, Liu J, Kim KS, Kern P, Chang H-C, Wang J-H, Reinherz EL. Differential thymic selection outcomes stimulated by focal structural alteration in peptide/MHC ligands. Proc Natl Acad Sci USA, 1998; 95:10061-10066. 29. Kern PS, Teng M-K, Smolyar A, Liu J-H, Liu J, Hussey RE, Spoerl R, Chang H-C, Reinherz EL, Wang J-H. Structural basis of CD8 co-receptor function revealed by crystallographic analysis of a murine CD8αα ectodomain fragment in complex with H-2Kb. Immunity 1998; 9:519-530. 30. Wang JH, Smolyar A, Tan K, Liu JH, Kim M, Sun ZJ, Wagner G, Reinherz EL. Structure of a heterophilic adhesion complex between human CD2 and CD58 (LFA-3) counter-receptors. Cell 1999; 97:791-803. 31. Green N, Rosebrook J, Cochran N, Tan K, Wang J-H, Springer TA, Briskin MJ. Structural analysis of MAdCAM-1/a4b7 interactions reveals significant binding determinants in both the first and second immunoglobulin domains. Cell Adhesion and Communication, 1999; 7:167-181. 32. Reinherz EL, Tan K, Tang L, Kern P, Liu J-H, Xiong Y, Hussey RE, Smolyar A, Hare B, Zhang R, Joachimiak A, Chang H-C, Wagner G, Wang J-H. The structure of a T cell receptor in complex with peptide and MHC class II. Science 1999; 286:1913-1921. 33. Sasada T, Ghendler Y, Wang J-H, Reinherz EL. Thymic selection is influenced by subtle structural variation involving the p4 residue of an MHC class I-bound peptide. Eur J Immunol. 2000; 30:1281-1289. 34. Taraszka KS, Higgins JMG, Tan K, mandelbrot DA, Wang J-H, Brenner MB. Molecular basis for leukocyte integrin αEβ7 adhesion to epithelial (E-) cadherin. J Exp Med. 2000; 191:1555-1567. 35. Higgins JMG, Cernadas M, Tan K, Irie A, Wang J-H, Takada Y, Brenner MB. The role of α and β chains in integrin recognition by β7 integrins. J Biol Chem. 2000; 275: 25652-25664. 36. Wang J-H, Meijers R, Xiong Y, Liu J-H, Sakihama T, Zhang R, Joachimiak A, Reinherz EL. Crystal structure of the human CD4 N-terminal two domain fragment complexed to a class II MHC molecule. Proc Natl Acad Sci USA 2001; 98:10799-10804. 37. Kim MK, Sun Z-Y J, Byron O, Campbell G, Wagner G, Wang J-H, Reinherz EL. Molecular dissection of the CD2-CD58 counterreceptor interface identifies CD2 Tyr86 and CD58 Lys34 residues as the functional 'hot spot'. J Mol Biol. 2001; 312:711-20. 38. Sasada T, Touma M, Chang H-S, Clayton LK, Wang J-H, Reinherz EL. Involvement of the TCR Cβ FG loop in thymic selection and T cell function. J Exp Med. 2002; 195: 1419-31.
  9. 9. 39. Jing H, Takagi J, Liu J-H, Lindgren S, Zhang R, Joachimiak A, Wang J-H, Springer TA. Archaeal surface layer proteins contain β propeller, PKD and β helix domains and are related to metazoan cell surface proteins. Structure 2002; 10:1453-1464. 40. Tan K., Zelus BD, Meijers R, Liu J-H, Bergelson JM, Duke N, Zhang R, Joachimiak A, Holmes KV, Wang J-H. Crystal structure of murine sCEACAM1a[1,4]: a coronavirus receptor in the CEA family. EMBO J. 2002; 21:2076-2086. 41. Tan K, Duquette M, Liu J-H, Dong Y, Zhang R, Joachimiak A, Lawler J, Wang J-H. Crystal structure of the TSP-1 type 1 repeats: A novel layered fold and its biological implication. J Cell Biol. 2002; 159:373-382. 42. Sasada T, Yang Y, Lai C-C, Clayton LK, Liu J-H, Parisini E, Wang J-H, Reinherz EL. Disparate peptide-dependent thymic selection outcomes in β2M versus• TAP-1 deficient mice: Implications for repertoire formation. Eur J Immunol. 2003; 33:368-380. 43. Liu Y, Xiong Y, Naidenko OV, Liu J-H, Zhang R, Joachimiak A, Kronenberg M, Cheroutre H, Reinherz EL, Wang J-H. The crystal structure of a TL/CD8 αα complex at 2.1 resolution: Implications for modulation of T cell activation and memory. Immunity 2003; 18:205-215. 44. Shimaoka M, Xiao T, Liu J-H, Yang Y, Dong Y, Jun C-D, McCormack A, Zhang R, Joachimiak A, Takagi J, Wang J-H, Springer TA. Structures of the aL I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation. Cell 2003; 112: 99-111. 45. Takagi J, Yang Y, Liu J-H, Wang J-H, Springer TA., Complex between nidogen and laminin fragments reveals a paradigmatic β-propeller interface. Nature 2003; 424: 969-974. 46. Yang Y, Jun C-D, Liu J-H, Zhang R, Jochimiak, A, Springer TA, Wang J-H. Structural basis for dimerization of ICAM-1 on the cell surface. Mol. Cell. 2004, 14:269-276. 47. Xiao T, Takagi J, Coller BS, Wang J-H, Springer TA. Structural basis for allostery in integrins and binding of ligand-mimetic therapeutics to the platelet receptor for fibrinogen. Nature, 2004; 432: 59-67. 48. Qiao Z, Kim M, Reinhold B, Montefiori D, Wang J-H, Reinherz EL. Design, expression and immunogenicity of a soluble HIV trimeric envelope fragment adopting a prefusion gp41 configuration. J Biol Chem. 2005; 280:23138-23146. 49. Song G, Yang Y, Liu J-H, Casasnovas JM, Shimaoka M, Springer TA, Wang J-H. Atomic resolution view of ICAM recognition revealed in a 1.65Å structure of a complex between the binding domains of ICAM-3 and integrin αLβ2. Proc Natl Acad Sci USA. 2005; 102: 3366-3371 50. Attinger A, Devine L, Wang-Zhu, Y, Martin, D, Wang J. H, Reinherz EL, Kronenberg M, Cheroutre H, Kavathas P. Molecular basis for the high affinity interaction between the thymic leukemia antigen and the CD8αα molecule. J Immunol., 2005; 174: 3501-7
  10. 10. 51. Meijers R, Lai C-C, Yang Y, Liu J-H, Zhong W, Wang J-H, Reinherz EL. Crystal structures of murine MHC class I H-2 Db and Kb molecules in complex with CTL epitopes from influenza A virus: Implications for TCR repertoire selection and immunodominance. J. Mol. Biol., 2005; 345: 1099-1110. 52. Chang HC, Tan K, Ouyang J, Parisini E, Liu J-H, Le L, Wang X, Reinherz EL, Wang J- H. Structural and mutational analyses of a CD8αβ heterodimer and comparison with CD8αα homodimer. Immunity, 2005; 23: 661-671 53. Tan K, Duquette M, Liu J-H, Zhang R, Joachimiak A, Wang J-H, Lawler J. The Structures of the Thrombospondin-1 N-terminal domain (TSPN-1) and its complex with a synthetic Pentameric Heparin. Structure, 2006; 14: 33-42 54. Meijers R, Puettmann-Holgado R, Skiniotis G, Liu J-H, Walz T, Wang J-H, Schmucker D., Structural Basis of Dscam Isoform specificity, Nature, 2007; 449: 487-491 55. Parisini E, Higgins JMG, Liu J-H, Brenner MB, Wang J-H. The crystal structure of human E-cadherin domains 1 and 2, and comparison with other cadherins in the context of adhesion mechanism, J. Mol. Biol., 2007; 373: 401-411 56. Tan K, Duquette M, Liu J-H, Shanmugasundaran K, Joachimiak A, Gallagher JT, Rigby AC, Wang J-H and Lawler J. Heparin-induced cis- and trans-dimerization modes of the thrombospondin-1 N-terminal domain. J. Biol. Chem. 2008, 283: 3932-3941 57. Sun ZYJ, Oh KL, Kim M, Yu J, Brusic V, Song L, Qiao Z, Wang J-H, Wagner G, Reinherz EL. HIV-1 broadly neutralizing antibody extracts its epitope from a kinked gp41 ectodomain region on the viral membrane. Immunity, 2008, 28: 52-63 58. Grotenbreg GM, Roan NR, Guillen E, Meijers R, Wang J-H, Bell GW, Starnbach MN, Ploegh HL. Discovery of CD8+ T-cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers. Proc Natl Acad Sci USA. 2008; 105: 3831-35 59. Ng A., Zhang H., Tan K., Li Z., Liu J-H., Chan P., Li S-M., Chan W-Y., Au S., Joachimiak A., Walz T., Wang J-H., Shaw P-C. Structure of the influenza H5N1 nucleoprotein: implications for RNA binding, oligomerization and vaccine design. FASEB J., 2008, 22: 3638-3647 60. Tan K., Duquette M., Liu J-H., Joachimiak A., Lawler J., Wang J-H., The Crystal Structure of the Heparin-binding Reelin-N Domain of F-spondin. J. Mol. Biol., 2008; 381: 1213-1223 61. Zhang H., Casasnovas JM., Jin M., Liu J-H., Gamberg CG., Springer TA., Wang J-H., An unusual allosteric mobility of the C-terminal helix of a high affinity αL integrin I domain variant bound to ICAM-5. Mol. Cell, 2008; 31: 432-437
  11. 11. Reviews: 1. Lewis M, Jeffrey A, Wang JH, Ladner R, Ptashne M, Pabo CO. Structure of the operator binding domain of lambda repressor: Implication for DNA recognition and gene regulation. Cold Spring Harbor Symp. Quant. Biol. 1983; 47:435-440. 2. Wang JH. How do proteins recognize DNA. Progress in Bicohem. and Biophys (China). 1984; 1:2-8. 3. Lewis M, Wang JH, Pabo CO. Structure of the operator binding domain of lambda- repressor. In: (Jurnak & McPherson, eds), Structure of Biological Macromolecules and Assemblies, Wiley & Sons, 1985; 2:266-287. 4. Wang JH. Protein structure and gene structure. The chemistry of life. (China) 1985; 5:3-4. 5. Stuart D, Liang DC, Dai JB, Todd R, Lou MZ, You JM, Li JY, Wang JH. The solution of the structure of monomeric insulin derivative by the use of anomalous scattering from thenative crystal. In: Structural biological applications of X-ray absorption, scattering and diffraction, Academic Press. 1986; 315-328. 6. Wang JH. How to design and modify protein molecules. In Progress in Biochem. and Biophys. (China) 1988; 2:144-145. 7. Wang JH, Dong YC. Trichosanthin: from Chinese herb medicine to ribosome inactivating protein. In: (Tsou CL, ed) Current Biochemical Research in China, Academic Press, 1989; 25-37. 8. Wang JH. DNA topoisomerase. In: Gene regulation and enzymology in biotechnology. Academic Press: China, 1990; 123-141. 9. Harrison SC, Wang JH, Yan YW, Garrett T, Liu JH, Moebius U, Reinherz EL. Structure and interaction of CD4. Cold Spring Harbor Symp. on Quant. Biol. #57, CSH Laboratory Press, 1992; 541-548. 10. Harrison SC, Wang JH, Yan YW, Garrett T, Liu JH, Moebius U, Reinherz EL. Structure and interactions of CD4. Proc. of Novo Nordisk Foundation Symp. No. 6 Leukocyte Adhesion, Basic and Clinical Aspects (Gahmberg CG, Mandrup-Poulsen T, Wogensen Bach L, Hokfelt B, eds), Holland: Elsevier, 1992; 449-473. 11. Wang J-H, Springer TA. Structural specializations of immunoglobulin superfamily members for adhesion to integrins and viruses. Immunol Rev. 1998; 163: 197-215. 12. Wang JH, The insulin connection: Dorothy Hodgkin and the Beijing Insulin Group. Trends in Biochem. Sci. 1998; 23: 497-500 13. Wang J-H, Reinherz EL. Structural basis of cell-cell interactions in the immune system. Curr Op Struct Biol. 2000; 10:656-661. 14. Wang J-H, Reinherz EL. Structural basis of T cell recognition of peptides bound to MHC molecules. Mol Immunol. 2001; 38:1039-1049.
  12. 12. 15. Wang J-H. Protein recognition by cell surface receptors: physiological receptors versus virus interactions. Trends in Biochemical Sciences. 2002; 27:122-126. 16. Wang, J-H, Eck, MJ. Assembling atomic resolution views of the immunological synapse. Current Opinion in Immunol. 2003; 15: 286-293. 17. Springer TA, Wang J-H., The three-dimensional structure of integrins and their ligands, and conformational regulation of cell adhesion. Advances in Prot. Chem. 2004; Volume 68 ed. Garcia, K. C., Elsevier, San Diego, CA. pp 29-63. 18. Wang J-H., Molecular mechanism of cell-cell recognition. Progress in Biochem.. and Biophys., 2004; 31: 385-392. 19. Wang J-H, Mallis R.J. and Reinherz, E.L., Immunodominant-peptide recognition: Beta testing TCRαβ. Immunity, 2008; 28: 139-141
  13. 13. Research projects during the last three years: NIH HL 48675, Integrins and modular surface proteins in vasculature, program leader: Timothy Springer, Proj. 2: Structure and interaction of IgSF and integrin families, P.I. Jia-huai Wang Project period: 9/20/00-8/31/05; 9/1/05-8/31/10 Specific aims: The goal of this project is to study the structures of IgSF proteins on endothelium, carry out structural dissection of a leukocyte integrin molecule and study the interaction of these IgSF proteins and integrins Claudia Adams Barr Program Award: Structure and interactions of nucleoprotein (NP) of influenza virus H5N1 (PI: Jia-huai Wang). Project period: 7/1/07-6/30/09 Specific aims: The long-term goal of this project is to carry out systematic structural analyses of interactions of H5N1 NP with various viral molecules as well as proteins from the host cell. The ultimate aim is to formulate therapeutic and preventive strategies to interfere with these interactions. NIH AI43649, HIVRAD: Structural approaches to vaccine development; PI: E. Reinherz, Investigator: Proj. 1: Structure-based design for Nabs against SIV/HIV Project period: 9/30/02-7/31/07 Specific aims: Development of mini-protein variants of SIV and HIV envelope proteins for vaccine design aimed at generating broadly neutralizing antibody responses Completed: NIH 2RO1 AI25231, Molecular genetics of the receptor for MVH; P.I.: K. Holmes, Wang, subcontract leader, Project period:7/1/03-12/31/07 Specific aims of project: To understand at the molecular level what determines the species specificity of coronavirus NIH HL 49081, Structure and function of thrombospondin-4, PI: J.W. Lawler, Wang, subcontract leader, Project period: 4/15/04-3/31/08 Specific aims: To determine the structure and function of two members of the subgroup B thrombospondins, cartilage oligomeric matrix protein and thrombospondin-4. NIH GM56008, MHCI-restricted TCR recognition: Structural basis, P.I. Jia-huai Wang Project period: 1/1/98-12/31/04 (included one year no cost extension) Specific aims: X-ray crystal structural determination of ligated and unligated (with VSV8/K b complex) N15 TCR and other TCRs to unravel molecular mechanisms of TCR recognition for the MHC class I-restricted system. NIH HL 68003, Structure and function of thrombospondin-1, (PI: J.W. Lawler) Wang, subcontract leader, Project period: 4/1/02-3/31/06 Specific aims: Crystal structure determination of type 1 repeat and its complex with ligand CD36
  14. 14. NIH 1U19 AI57330, CCTR program project.: Protective orthopox immunization in normals and patients with cancer or eczema (program leader E.L. Reinherz). PI for Pilot project 2 Project period:9/30/03-8/31/08 (1.5 year for pilot project) Specific aims: Analysis of human immunity to orthopox viruses NIH 1RO1 AI50900, Thymic vaccination: Manipulating the T cell repertoire, PI: E. Reinherz Project period: 3/19/01-2/28/05 (includes one year no cost extension) Specific aims: Approaches to development of vaccines against mouse infectious pathogens (LCMV, VSV, influenza A) NIAID 1PO1 AI/MH43649, Novel HIV therapies - IPCP program project (program leader E.L. Reinherz), Proj. 2: Structural biology of HIV envelope interactions, P.I.: S. Harrison Project period: 8/1/98-7/31/02 Specific aims: Crystallography, site-directed mutagenesis and functional studies of CD4 and CD4-gp120 interaction