GABRIEL – Course/Workshop
Immunological methods for epidemiological studies followed by specialized
analysis for immunological data
Dates: 19-22 November 2007
Location: Leiden University Medical Center, Leiden, the Netherlands
Christine Benn (Staten Serum Institue, Denmark)
Jeanine Houwing-Duistermaat (Leiden University Medical Center, The Netherlands)
Laura Rodrigues (London School of Hygiene and Tropical Medicine, United Kingdom)
Hae-Won Uh (Leiden University Medical Center, The Netherlands)
Maria Yazdanbakhsh (Leiden University Medical Center, The Netherlands)
Aim: to teach those interested in epidemiological studies to use simple, standardized,
feasible, high capacity and cutting edge techniques to understand the immune system at
the cellular and molecular level.
First part of the course (19,20th Nov) will consist of audio visually assisted teaching of
blood culture methods, FACS and cytokine analysis as well as mRNA isolation for gene
expression studies. Teaching material will provide the participants with tools to study
Th1, Th2, Th17 and Treg cells in cross sectional or longitudinal studies whereby the data
can then be used to understand the effect that exogenous factors have on the
development of the immune system.
Second part of the course (21,22nd Nov) will focus on data generated from
immunoepidemiological studies and how to analyze them. Immunological data have
characteristics that need special statistical techniques for appropriate analyses. A data
set will be used by participants as an example to explore different methods of analysis.
Lectures will be given by experts in the field in the morning and practical sessions on
data analysis will be held in the afternoon.
Immunological methods Data analysis
Bart Everts (LUMC) Christine Benn (SSI)
Alwin van der Ham (LUMC) Jeanine Houwing (LUMC)
Franca Hartgers (LUMC) Nico Nagelkerke (UAEU)
Yvonne Kruize (LUMC) Laura Rodrigues (LSHTM)
Hermelijn Smits (LUMC) Stijn Vansteelandt (GENTU)
Sunny Sapthu (LUMC) Hae-won Uh (LUMC)
Erliyani Sartono (LUMC)
Maria Yazdanbakhsh (LUMC)
Max number of participants: 40
Deadline for GBRIEL members registration: 30th Sept 2007
Information: M.Yazdanbakhsh@lumc.nl (subject : immunology workshop)
[Please select GABRIEL member box when it comes to registration page that requests
payment- then you will be exempted from payment]
The workshop consists of two days of Immunological Methods (19th and 20th of November 2007)
followed by two days of Statistical Analysis of Immuno-epidemiological data (21st and 22nd of
November 2007). Two different locations will be used as follows:
19 and 20 November
Location: Leiden University Medical Center (www.lumc.nl/english/general/addresses.html )
9.00-12.00 Leiden University Medical Center : Lecture Hall 4
13.00-17.00 Laboratories at Leiden University Medical Center (details of which will be given
upon your arrival.
Registration 8.30 – 9.00 in front of Lecture Hall 4
21 and 22 November
Location: Leiden University Plexus Study Center ( www.plexus.leidenuniv.nl )
Golden tulip Leiden www.goldentulipleidencentre.nl (10 rooms in reservation) participants make
their own reservation
Holiday Inn Leiden www.holiday-inn-leiden.nl (10 rooms in reservation) participants make their
LUMC How to get there:
By public transport
The LUMC is situated at the back of Leiden Central Station and is within walking distance from
the station. All (region) busses stop at the bus station at the front of the train station.
A few buses stop at the Bargelaan stop near the LUMC. All buses stop at the front of Leiden
Central Station. Within walking distance from the LUMC you will also find the Educational and
Student Affairs Office and the Postgraduate Training Office (Boerhaave Committee).
For current public transport information call the Public Transport-travel information number
0900-9292 (calls are 75 cents per minute) or use the Public Transport Guide on the Internet at
Coming from direction Rotterdam (A13):
- Follow the A13 and after that the A4 direction Amsterdam until exit 6a (Zoeterwoude-
Rijndijk, Leiden-Oost). At the end of the exit make a left turn. At the end of the road make
a right turn. Then follow the signposts "H-ziekenhuizen".
Coming from the direction Utrecht (A12, N11):
- Follow the A12 and take the exit Leiden (N11) at junction Bodegraven. At the end of the
N11 make a right turn. Then follow the signposts "H-ziekenhuizen".
Coming from the direction Den Haag (N44, A44):
- Follow the N44 (later A44) until exit 8 (Leiden, Noordwijk, Katwijk). At the end of the exit
make a right turn. Then follow the signposts "H-ziekenhuizen".
Coming from the direction Amsterdam (A4, A44):
- Follow the A4 and after that the A44 direction Leiden-West. Follow the A44 until exit 8
(Leiden, Noordwijk, Katwijk, N206). After the exit make a left turn and drive under the
viaduct. Then follow the signposts "H-Ziekenhuizen".