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Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
Diagnosing HIV in mothers and infants
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Diagnosing HIV in mothers and infants

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  • 1. PROVING HIV INFECTION IN MOTHERS AND BABIES The Perth Group Western Australia www.theperthgroup.com www.virusmyth.net/aids/perthgroup [email_address] This file has speaker notes
  • 2. PROVING HIV INFECTION IN MOTHERS AND BABIES A more detailed analysis of this problem is available at www.virusmyth.net/aids/perthgroup In the Nevirapine presentation
  • 3. DIAGNOSIS OF HIV INFECTION IN MOTHERS ANTIBODY TESTS
    • Blood sample
    • HIV proteins
    • Technique (ELISA and Western blot)
  • 4. HIV PROTEINS Montagnier 1983 & Gallo 1984 Claimed to have isolated/purified a retrovirus HIV by separating it from everything else including proteins in cell cultures Barré-Sinoussi, F et al. (1983). “Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).” Science 220 : 868-71. Gallo, RC et al . (1984). “Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.” Science 224 : 500-503 .
  • 5. WHY NO PICTURES OF “PURIFIED VIRUS”? Montagnier videotaped interview at Pasteur Institute 18 th July 1997 Copyright to and available from Djamel Tahi <djamel@hotmail.com> Text of interview published in Continuum (1998) 5: 30-34. www.virusmyth.com/aids/data/dtinterviewlm.htm
  • 6. No particles “typical of retroviruses” in “purified virus” Montagnier interview at Pasteur Institute July 1997 Continuum (1998) 5: 30-34. www.virusmyth.com/aids/data/dtinterviewlm.htm “ Did Gallo purify? “ Gallo?..I don’t know if he really purified. I don’t believe so” MONTAGNIER ON MONTAGNIER AND GALLO
  • 7. “ I repeat, we did not purify” Montagnier interview at Pasteur Institute July 1997 Continuum (1998) 5: 30-34. www.virusmyth.com/aids/data/dtinterviewlm.htm MONTAGNIER DID NOT ISOLATE/PURIFY HIV
  • 8. Bess JW, Gorelick RJ, Bosche WJ, Henderson LE, Arthur LO. Microvesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations. Virology 1997; 230: 134-144.
  • 9. HIV PROTEINS IN NORMAL HUMAN PLACENTA p18/p24/p120 “ Placentae from 25 normal term pregnancies were collected by vaginal delivery...Antigens gp120 and p17 were identified in normal chorionic villi…Antigen p24…in villous mesenchymal cells...localized to HLA-DR positive cells” Faulk, WP et al (1991). “HIV proteins in normal human placentae.” American Journal of Reproductive Immunology 25 : 99-104.
  • 10. THE “HIV” PROTEINS p41/p120/p160 Montagnier considers p41 to be cellular actin p160, p120 in “HIV” WB are oligomers of p41 Pinter AW et al (1989). “Oligomeric structure of gp41, the transmembrane protein of human immunodeficiency virus type 1 Journal of Virology 63 : 2674-9. Zolla‑Pazner S et al (1989). Reinterpretation of Human Immunodeficiency virus Western Blot patterns. NEJM 320 :1280‑1281.
  • 11.
  • 12. “ We agree that you can come to the conclusion from gel electrophoresis patterns that there are only quantitative differences between HIV and [cellular] microvesicles” Bess et al National Cancer Institute USA Bess, J. W., R. J. Gorelick, et al. (1997). Email correspondence August 2000 re Microvesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations. Virology 230: 134-144 “ We have been unsuccessful in separating microvesicles from HIV”
  • 13.
  • 14. Immune complexes, rheumatoid factor, anti‑cardiolipin, anti‑nuclear factor, anti‑cellular, anti‑platelet, anti‑red cell, anti‑actin, anti‑DNA, anti‑tubulin, anti‑thyroglobulin, anti‑albumin, anti‑myosin, anti‑trinitrophenyl anti‑thymosin, anti-lactoferrin, anti-TNF- α, anti-beta-2 glycoprotein I, anti-prothrombin, anti-neutrophil cytoplasmic, anti-ssDNA, anti-RNA, anti-histones, anti-nuclear antigen SS-A, anti-mitochondrial,anti-reticulin, anti-smooth muscle, anti-gut epithelial cell, anti-lymphocytic ganglioside, anti-Fab, anti-protein S, anti-brain proteins, anti-synthetic peptides of ubiquitinated histone H2A, anit-Sm-D antigen, anti-U1-A RNP antigen, anti-60 kD SSA/Ro antigen, anti-histone H1 and anti-histone H2B antibodies. Anti‑lymphocyte auto‑antibodies in 87% of seropositives. AUTO-ANTIBODIES IN HIV/AIDS PATIENTS
  • 15.
    • Hypergammaglobulinaemia predicts seropositivity *
    • Antibodies directed against fungi and mycobacteria cross-react with HIV proteins
    • Fungal and mycobacterial diseases are the indicator diseases present in 90% of AIDS patients
    • Kashala et al 1995 advised caution using Western blot in high prevalence mycobacterial areas
    ANTIBODY CROSS-REACTIVITY *Brenner, B., S. Schwartz, et al. (1991). “The prevalence and interaction of human immunodeficiency virus and hepatitis B infections in Israeli hemophiliacs.” Israel journal of medical sciences 27 : 557-561.
  • 16.
  • 17. ENV POL GAG p160 p120 p32 AFR AUS FDA RCX CDC 1 CDC 2 CON MAC UK FRA GER ANY 2 HIV WESTERN BLOT STRIP p32 p32 p32 p24 p24 ANY 1 ANY 1 p160/ p120 AND p41 p160/ p120 OR p41 p160/ p120 OR p41 OR ALL 3 OR ANY 1 GAG OR POL ANY 1 ANY 1 ANY STRONG BAND 3 WEAK BANDS ANY 3 GAG OR POL p24 AND AND AND AND ANY 1 ANY 1 OR ANY 1 ANY 1 ANY 1 p24 NONE ESSEINTIAL p41 p68 p53 p55 p39 p24 p18
  • 18. GOLD STANDARD HIV ITSELF HIV ISOLATION/PURIFICATION
  • 19. ANTIBODY DIAGNOSIS IN CHILDREN Additional problem Persistence of maternal antibodies in infant
  • 20. Mother-to-child transmission of HIV infection. The European Collaborative Study. (1988). Lancet ii : 1039-43.
  • 21. “ In adults, adolescents, and children infected by other than perinatal exposure, plasma viral RNA nucleic acid tests should NOT be used in lieu of licensed HIV screening tests (e.g., repeatedly reactive enzyme immunoassay)” (emphasis in original). “ HIV nucleic acid (DNA or RNA ) detection tests are the virologic methods of choice to exclude infection in children aged <18 months” (“Positive results on two separate specimens ) ( emphasis added ). CDC 2000 Revised AIDS Surveillance Definition   Centers for Disease Control and Prevention. Mortality and Morbidity Weekly Reports 1999;48 (RR-13):1-27, 29-31.
  • 22. “ The Amplicor HIV-1 [RNA] Monitor test is not intended to be used as a screening test for HIV-1 or as a diagnostic test to confirm the presence of HIV-1 infection” Roche Diagnostic Systems, 06/96, 13-08088-001. Packet Insert Roche Laboratories
  • 23.
    • Recommendations:
    • 1. The public should be informed there is a scientific problem.
    • A small, international conference or a congressional review of the scientific evidence for and against the HIV theory of AIDS. In the presence of highly regarded, international, disinterested adjudicators, not all scientists, and of Nobel Laureate standard. Acceptable to both sides of the debate and the conveners.
    • 3. Funding dissident scientists to undertake experiments to prove or disprove the HIV theory. A relatively inexpensive undertaking.
    • 4. To be conducted and concluded this year.
    • 5. A moratorium on HIV testing until results are known.
  • 24.  

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