Microscopy

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K12 / GRADE 7 / SECOND QUARTER / MICROSCOPY

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Microscopy

  1. 1. Microscopy What is a microscope?
  2. 2. Microscope  Microscope is a tool which can help you see tiny objects and living organisms. It makes them look bigger.  This ability of the microscope is called its magnifying power or magnification.
  3. 3. Microscope  The microscope also has the capacity to distinguish small gaps between two separate points which humans cannot distinguish. It is called its resolving power or resolution.
  4. 4. Light microscope  Light microscope uses diffused light from the sun or artificial light to illuminate the object to be observed.
  5. 5. Microscopy What are the parts of the microscope?
  6. 6. 1. Arm  Supports the body tube.
  7. 7. 2. Base  Supports and stabilizes the microscope.
  8. 8. 3. Eyepiece / ocular lens  Magnifies image produced by objective lens.
  9. 9. 4. Body Tube  Maintains the proper distance between the objective and ocular lenses.
  10. 10.  Moves stage up and down a large amount for FOCUSING. 5. Coarse adjustment knob
  11. 11.  Moves stage up and down a tiny amount to SHARPEN the image. 6. Fine adjustment knob
  12. 12. 7. Light source  (lamp or mirror) Provides light for viewing the slide.  Projects light UPWARDS through the diaphragm, the SPECIMEN, and the LENSES.
  13. 13. 8. Diaphragm  Controls the amount of light passing through the slide.
  14. 14.  Stage clips - hold the slide in place.  Stage - Supports the slide being viewed. 9. Stage and stage clips
  15. 15. 10. Objective lenses  Focus and magnify light coming through the slide.
  16. 16.  Rotates to allow use of different power objectives. 11. Revolving nosepiece
  17. 17.  Supports the arm and controls the body of the microscope. 12. Inclination joint
  18. 18. Basic Microscope Technique Rules to Follow 1. If you must carry a microscope, always hold it with one hand on the arm and the other under the base. 2. Always lower the stage or raise the objectives all the way before placing a slide under the objectives. 3. Always begin working with the LOW POWER (shortest) objective first. 4. Observe the slide from the side, not looking through the eye piece, when using the coarse focus to avoid running the objective lens into the slide. 5. Never use the coarse focus adjustment when on the medium or high power objectives. Focus on low power first and then rotate the higher power objective into place. Make final focus adjustments with the fine focus adjustment.
  19. 19. Answer Each Question 1. The lens closest to your eye when you use a microscope is the __________. 2. When using the microscope, always begin work with the __________ objective first. 3. Which knob should you never use while on medium or high power? Explain why. 4. The lens closest to the slide is the ___________. 5. When using the coarse focus knob, how should you be view the slide? Explain why. 6. Carry the microscope with one hand on the ________. and the other on the __________.
  20. 20. End of discussion

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