Ethanol from Xylose

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Seminar given to grad student @ Dept of Biotechnology, KKU in Jan 2008.

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Ethanol from Xylose

  1. 2. <ul><li>Lignocellulose </li></ul>Lignin Glucose Xylose Other sugars Acid & enzyme hydrolysis Strain selection Co-cultivation in glucose/xylose medium Ethanol from xylose : free cells Ethanol from xylose : immobilised cells
  2. 3. <ul><li>Saccharomyces cerevisiae </li></ul><ul><li>Zymomonas mobilis </li></ul>
  3. 4. <ul><li>Kluyveromyces marxianus </li></ul><ul><li>Candida shehatae </li></ul>Candida shehatae Ethanol Production phase
  4. 5. <ul><li>Z. mobilis and C. shehatae in 2-stage cultivation </li></ul>
  5. 6. <ul><li>C. shehatae in fermenter at various pO 2 levels (xylose concentration) </li></ul>25% 25%
  6. 7. <ul><li>C. shehatae in fermenter at various pO 2 levels (biomass concentration) </li></ul>
  7. 8. <ul><li>C. shehatae in fermenter at various pO 2 levels (ethanol concentration) </li></ul>
  8. 9. <ul><li>Ongoing experimental works </li></ul><ul><li>Use C. shehatae biomass immobilised in Ca-alginate beads </li></ul><ul><li>Varying cell concentration in beads </li></ul><ul><li>Varying alginate concentration </li></ul><ul><li>Repetitive cultivation </li></ul>
  9. 10. <ul><li>Acid Hydrolysis </li></ul><ul><li>Enzymatic hydrolysis </li></ul>NH 4 OH Dilute acids (HCl, H 2 SO 4 ) Conc acids (HCl, H 2 SO 4 ) Cellulose, cellobiase Lignin removal Hemicellulose hydrolysis Cellulose hydrolysis
  10. 11. <ul><li>Collaboration with </li></ul><ul><li>Dr. Atcha Boonmee, Microbiology, KKU </li></ul><ul><li>Cloning of genes responsible for xylose utilisation </li></ul><ul><li>C. shehatae as gene donor </li></ul><ul><li>S. cerevisiae as host cells </li></ul>
  11. 12. <ul><li>Lactic acid production process improvement </li></ul><ul><ul><li>Integration of external modules for lactic acid removal : improvement towards continuous production </li></ul></ul><ul><ul><li>Downstream process : recovery without solid waste (collaboration with Dr. Arthit Neramittagapong, Chem. En., KKU) </li></ul></ul><ul><li>Xylose utilisation for production of chemicals </li></ul>
  12. 14. <ul><li>University library </li></ul>
  13. 15. <ul><li>Walkway between Electrical Engineering and Mechanical Engineering </li></ul>
  14. 16. <ul><li>Overlook from middle campus </li></ul>
  15. 17. <ul><li>Biological Sciences Building </li></ul>
  16. 18. <ul><li>One of lab bench in my G17 lab </li></ul>
  17. 19. <ul><li>In G17 with Martin, my lab manager </li></ul>
  18. 20. <ul><li>In G17 lab with Nick </li></ul>
  19. 21. <ul><li>Also in G17 lab with Caine and Tony </li></ul>
  20. 22. <ul><li>Pilot plant as part of my lab </li></ul>
  21. 23. <ul><li>Another view of the lab </li></ul>
  22. 24. <ul><li>Trichoderma harzianum cultivation in 5-L fermenter </li></ul>
  23. 25. <ul><li>Normal set-up of batch fermentation system </li></ul>
  24. 26. <ul><li>Set-up for ion exchange resin regeneration loop </li></ul>
  25. 27. <ul><li>Set-up of fermentation system with electrodialysis (1) </li></ul>
  26. 28. <ul><li>Set-up of fermentation system with electrodialysis (2) </li></ul><ul><li>Electrodialysis system </li></ul>
  27. 30. May you all cross the bridge Over all the obstacles Towards the success you wish … HAPPY NEW YEAR 2008 …

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