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Ethanol from Xylose
 

Ethanol from Xylose

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Seminar given to grad student @ Dept of Biotechnology, KKU in Jan 2008.

Seminar given to grad student @ Dept of Biotechnology, KKU in Jan 2008.

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    Ethanol from Xylose Ethanol from Xylose Presentation Transcript

    •  
      • Lignocellulose
      Lignin Glucose Xylose Other sugars Acid & enzyme hydrolysis Strain selection Co-cultivation in glucose/xylose medium Ethanol from xylose : free cells Ethanol from xylose : immobilised cells
      • Saccharomyces cerevisiae
      • Zymomonas mobilis
      • Kluyveromyces marxianus
      • Candida shehatae
      Candida shehatae Ethanol Production phase
      • Z. mobilis and C. shehatae in 2-stage cultivation
      • C. shehatae in fermenter at various pO 2 levels (xylose concentration)
      25% 25%
      • C. shehatae in fermenter at various pO 2 levels (biomass concentration)
      • C. shehatae in fermenter at various pO 2 levels (ethanol concentration)
      • Ongoing experimental works
      • Use C. shehatae biomass immobilised in Ca-alginate beads
      • Varying cell concentration in beads
      • Varying alginate concentration
      • Repetitive cultivation
      • Acid Hydrolysis
      • Enzymatic hydrolysis
      NH 4 OH Dilute acids (HCl, H 2 SO 4 ) Conc acids (HCl, H 2 SO 4 ) Cellulose, cellobiase Lignin removal Hemicellulose hydrolysis Cellulose hydrolysis
      • Collaboration with
      • Dr. Atcha Boonmee, Microbiology, KKU
      • Cloning of genes responsible for xylose utilisation
      • C. shehatae as gene donor
      • S. cerevisiae as host cells
      • Lactic acid production process improvement
        • Integration of external modules for lactic acid removal : improvement towards continuous production
        • Downstream process : recovery without solid waste (collaboration with Dr. Arthit Neramittagapong, Chem. En., KKU)
      • Xylose utilisation for production of chemicals
    •  
      • University library
      • Walkway between Electrical Engineering and Mechanical Engineering
      • Overlook from middle campus
      • Biological Sciences Building
      • One of lab bench in my G17 lab
      • In G17 with Martin, my lab manager
      • In G17 lab with Nick
      • Also in G17 lab with Caine and Tony
      • Pilot plant as part of my lab
      • Another view of the lab
      • Trichoderma harzianum cultivation in 5-L fermenter
      • Normal set-up of batch fermentation system
      • Set-up for ion exchange resin regeneration loop
      • Set-up of fermentation system with electrodialysis (1)
      • Set-up of fermentation system with electrodialysis (2)
      • Electrodialysis system
    •  
    • May you all cross the bridge Over all the obstacles Towards the success you wish … HAPPY NEW YEAR 2008 …