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Wb cs count

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  • 1.  White blood cells or leukocytes are cells of the immune system which defend the body against both infectous disease and foreign materials.  The white blood cells have a rather short life cycle, living from a few days to a few weeks.  White cell count (WBC) is the total number of leukocytes in a volume of blood, expressed as thousands/µl.
  • 2.  Principle: Using diluent that destruct all blood cell leaving only WBCS intact
  • 3.  Equipment and reagents: 1. Hemacytometer and cover slip. 2. Glass pipette.
  • 4.  Reagents: 1. Turks' solution: which is formed of:  Glacial acetic acid  Gentian violet  Distilled water.  Specimen: EDTA- anticoagulated blood
  • 5.  Procedures: 1. Blood dilution: Add 10 µl of well mixed blood sample to 200µl of the diluting reagent. The dilution factor is 1:20. 2. leave the mix for 2 mins. till complete lyses of RBCs.
  • 6. 2- Charging :  Charge the hemocytometer between couver slip and the counting chamber.  Leave the cells to settle down. 3- Counting:  Count the WBCs in 4 1ry squares.  Count in L- shape manner.  Count under medium power.
  • 7.  ????? Cell / 4 squares.
  • 8.  Leukocytes µl of blood =  No. of cells x depth factor x dilution factor = no. of square counted
  • 9.  Leukocytosis is a condition characterized by an elevated number of white cells in the blood, which is usually due to:  Bacterial infection such as appendicitis, tonsillitis, ulcers and urinary tract infection  Leukemia.  Following exercise.  stress.
  • 10.  Leukopenia is a condition characterized by a decreased number of white cells in the blood, which is usually due to:  Viral disease such infectious hepatitis.  Some bacterial infections such as brucellosis.  Radio therapy and chemotherapy.
  • 11.  In case of counting WBCs and RBCs in poultry or fish blood, one is preferred to use Natt and Herrick solution.