Your SlideShare is downloading. ×
  • Like
Wb cs count
Upcoming SlideShare
Loading in...5

Thanks for flagging this SlideShare!

Oops! An error has occurred.


Now you can save presentations on your phone or tablet

Available for both IPhone and Android

Text the download link to your phone

Standard text messaging rates apply

Wb cs count



Published in Education , Technology , Business
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Be the first to comment
    Be the first to like this
No Downloads


Total Views
On SlideShare
From Embeds
Number of Embeds



Embeds 0

No embeds

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

    No notes for slide


  • 1.  White blood cells or leukocytes are cells of the immune system which defend the body against both infectous disease and foreign materials.  The white blood cells have a rather short life cycle, living from a few days to a few weeks.  White cell count (WBC) is the total number of leukocytes in a volume of blood, expressed as thousands/µl.
  • 2.  Principle: Using diluent that destruct all blood cell leaving only WBCS intact
  • 3.  Equipment and reagents: 1. Hemacytometer and cover slip. 2. Glass pipette.
  • 4.  Reagents: 1. Turks' solution: which is formed of:  Glacial acetic acid  Gentian violet  Distilled water.  Specimen: EDTA- anticoagulated blood
  • 5.  Procedures: 1. Blood dilution: Add 10 µl of well mixed blood sample to 200µl of the diluting reagent. The dilution factor is 1:20. 2. leave the mix for 2 mins. till complete lyses of RBCs.
  • 6. 2- Charging :  Charge the hemocytometer between couver slip and the counting chamber.  Leave the cells to settle down. 3- Counting:  Count the WBCs in 4 1ry squares.  Count in L- shape manner.  Count under medium power.
  • 7.  ????? Cell / 4 squares.
  • 8.  Leukocytes µl of blood =  No. of cells x depth factor x dilution factor = no. of square counted
  • 9.  Leukocytosis is a condition characterized by an elevated number of white cells in the blood, which is usually due to:  Bacterial infection such as appendicitis, tonsillitis, ulcers and urinary tract infection  Leukemia.  Following exercise.  stress.
  • 10.  Leukopenia is a condition characterized by a decreased number of white cells in the blood, which is usually due to:  Viral disease such infectious hepatitis.  Some bacterial infections such as brucellosis.  Radio therapy and chemotherapy.
  • 11.  In case of counting WBCs and RBCs in poultry or fish blood, one is preferred to use Natt and Herrick solution.