Your SlideShare is downloading. ×
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Wb cs count
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×
Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply

Wb cs count

636

Published on

Published in: Education, Technology, Business
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
636
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
23
Comments
0
Likes
0
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
No notes for slide

Transcript

  • 1.  White blood cells or leukocytes are cells of the immune system which defend the body against both infectous disease and foreign materials.  The white blood cells have a rather short life cycle, living from a few days to a few weeks.  White cell count (WBC) is the total number of leukocytes in a volume of blood, expressed as thousands/µl.
  • 2.  Principle: Using diluent that destruct all blood cell leaving only WBCS intact
  • 3.  Equipment and reagents: 1. Hemacytometer and cover slip. 2. Glass pipette.
  • 4.  Reagents: 1. Turks' solution: which is formed of:  Glacial acetic acid  Gentian violet  Distilled water.  Specimen: EDTA- anticoagulated blood
  • 5.  Procedures: 1. Blood dilution: Add 10 µl of well mixed blood sample to 200µl of the diluting reagent. The dilution factor is 1:20. 2. leave the mix for 2 mins. till complete lyses of RBCs.
  • 6. 2- Charging :  Charge the hemocytometer between couver slip and the counting chamber.  Leave the cells to settle down. 3- Counting:  Count the WBCs in 4 1ry squares.  Count in L- shape manner.  Count under medium power.
  • 7.  ????? Cell / 4 squares.
  • 8.  Leukocytes µl of blood =  No. of cells x depth factor x dilution factor = no. of square counted
  • 9.  Leukocytosis is a condition characterized by an elevated number of white cells in the blood, which is usually due to:  Bacterial infection such as appendicitis, tonsillitis, ulcers and urinary tract infection  Leukemia.  Following exercise.  stress.
  • 10.  Leukopenia is a condition characterized by a decreased number of white cells in the blood, which is usually due to:  Viral disease such infectious hepatitis.  Some bacterial infections such as brucellosis.  Radio therapy and chemotherapy.
  • 11.  In case of counting WBCs and RBCs in poultry or fish blood, one is preferred to use Natt and Herrick solution.

×