Microbes are ubiquitous. Tobe able to study microbes one need a pure culture of it Pure culture Single unadulterated species of cells. Meaning a colony of only 1 kind of microbe.
The survival and continued growth of microbes depend on adequate supply of nutrients and a favorable growth environment Medium A solution containing these nutrients. Culture media can either be: Liquid (broth medium) Semi-solid solid
1. Broth medium – lack solidifying agent (AGAR) AGAR – an extract of seaweed, complex carbohydrate composed mainly of galactose and without nutritional value.2. Semisolid medium Contain <1% agar
Colony Cluster of cells that originates from multiplication of a single cell and represents the growth of a single species of microbe. Pure culture Defined and well isolated colony
3. Solid media Hardened surface Prepared in 3 ways: 1. Agar slants – used to maintain pure culture. Agar is harden in a slanted position (test tube)
Agar deep used for the study of gaseous requirements of microbes
Agar plates harden in a large surface area usually in a petri dish
Broth Semisolid Agar slant Media solid Agar deep Agar plate Autoclave Bunsen burnerEQUIPMENT Culture tubes Petri dishes Wire loops & needles Transfer instruments Pipettes Waterbath Cultivation chambers Incubators Refrigerators Streak plate Pure culture techniques Pour plate Isolation of pure culture Spread plate
alsocalled a pipet, pipettor or chemical dropper isa laboratory instrument used to transport a measured volume of liquid.
•is a piece of laboratoryequipment, driven by a motor,which spins liquid samples athigh speed.
isa piece of laboratory equipment that provides a safe working area for people handling material potentially contaminated with pathogens (germs)
laminar flow cabinet is a carefully enclosed bench designed to prevent contamination of semiconductor wafers, biological samples, or any particle sensitive device.
isa device for controlling the temperature, humidity, and other conditions in which a microbiological culture is being grown.
A water bath is used in thelaboratory to allow a chemicalreaction to occur at anelevated temperature
Water distillers produce highlytreated and disinfected water forlaboratory usage. The distillationprocess removes minerals andmicrobiological contaminants andcan reduce levels of chemicalcontaminants.
is a technique widely used in molecular biology. As PCR progresses, the DNA generated is used as a template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified. It is possible to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece.
isa pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization.
is a photometer (a device for measuring light intensity) that can measure intensity as a function of the color, or more specifically, the wavelength of light.
a thin metal pincher that allows you to grasp test tubes at extreme temperatures.
a bottle or flask provided with one bent tube passing through the stopper for directing a stream of water on anything to be washed or rinsed and with means for forcing (as by blowing into a second tube passing through the stopper or by squeezing if the bottle is flexible) the water through the tube
a flask having a wide base, narrow neck, and conical form, convenient in laboratory experimentation for swirling liquids by hand.