Chapter 1 part 2 lab-equipment

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Chapter 1 part 2 lab-equipment

  1. 1.  Microbes are ubiquitous. Tobe able to study microbes one need a pure culture of it Pure culture  Single unadulterated species of cells.  Meaning a colony of only 1 kind of microbe.
  2. 2.  The survival and continued growth of microbes depend on adequate supply of nutrients and a favorable growth environment Medium  A solution containing these nutrients. Culture media can either be:  Liquid (broth medium)  Semi-solid  solid
  3. 3. 1. Broth medium – lack solidifying agent (AGAR)  AGAR – an extract of seaweed, complex carbohydrate composed mainly of galactose and without nutritional value.2. Semisolid medium  Contain <1% agar
  4. 4.  Colony  Cluster of cells that originates from multiplication of a single cell and represents the growth of a single species of microbe. Pure culture  Defined and well isolated colony
  5. 5. 3. Solid media  Hardened surface  Prepared in 3 ways: 1. Agar slants – used to maintain pure culture.  Agar is harden in a slanted position (test tube)
  6. 6.  Agar deep  used for the study of gaseous requirements of microbes
  7. 7.  Agar plates  harden in a large surface area usually in a petri dish
  8. 8. Broth Semisolid Agar slant Media solid Agar deep Agar plate Autoclave Bunsen burnerEQUIPMENT Culture tubes Petri dishes Wire loops & needles Transfer instruments Pipettes Waterbath Cultivation chambers Incubators Refrigerators Streak plate Pure culture techniques Pour plate Isolation of pure culture Spread plate
  9. 9.  alsocalled a pipet, pipettor or chemical dropper isa laboratory instrument used to transport a measured volume of liquid.
  10. 10. •is a piece of laboratoryequipment, driven by a motor,which spins liquid samples athigh speed.
  11. 11.  isa piece of laboratory equipment that provides a safe working area for people handling material potentially contaminated with pathogens (germs)
  12. 12.  laminar flow cabinet  is a carefully enclosed bench designed to prevent contamination of semiconductor wafers, biological samples, or any particle sensitive device.
  13. 13.  isa device for controlling the temperature, humidity, and other conditions in which a microbiological culture is being grown.
  14. 14. A water bath is used in thelaboratory to allow a chemicalreaction to occur at anelevated temperature
  15. 15. Water distillers produce highlytreated and disinfected water forlaboratory usage. The distillationprocess removes minerals andmicrobiological contaminants andcan reduce levels of chemicalcontaminants.
  16. 16.  is a technique widely used in molecular biology. As PCR progresses, the DNA generated is used as a template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified. It is possible to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece.
  17. 17.  isa pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization.
  18. 18.  is a photometer (a device for measuring light intensity) that can measure intensity as a function of the color, or more specifically, the wavelength of light.
  19. 19. a thin metal pincher that allows you to grasp test tubes at extreme temperatures.
  20. 20.  a bottle or flask provided with one bent tube passing through the stopper for directing a stream of water on anything to be washed or rinsed and with means for forcing (as by blowing into a second tube passing through the stopper or by squeezing if the bottle is flexible) the water through the tube
  21. 21. a flask having a wide base, narrow neck, and conical form, convenient in laboratory experimentation for swirling liquids by hand.

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