In Spec Spectrophotometric Standards Reference Guide 2011


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Reference Guide for UV Vis Standards

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In Spec Spectrophotometric Standards Reference Guide 2011

  1. 1. SpectrophotometricStandards Reference ® Guide NIST Traceable CHEMICALS Customizable ® No Mixing or Diluting
  2. 2. Ordering Information Customer service representatives and technical support are Contents Table of Contents available Monday through Friday from 7:30 am to 4:00 pm Continuous Improvement 4-5 Eastern Time. Orders may be placed by phone, e-mail. Every order receives an order confirmation that includes items or- In-Spec® UV Standards 5-6 dered, pricing, shipping method and estimated shipping date. In-Spec® Visible Standards 5-6 GFS Chemicals, Inc. In-Spec® Patent Blue Standards 8 800 Kaderly Drive In-Spec® Amaranth Red Standards 9 Columbus, OH 43228 In-Spec® BCA Purple Standards 10 800-838-5567 extension 121 (U.S. and Canada) 740-881-5989 (Fax) In-Spec® Nano-Kit 11 001-740-881-5501 (International) Wavelength Standards 12-13 (e-mail) Stray Light Filters 14 Potassium Dichromate 15 16 Custom Standards Opening an Account Amco Clear®Turbidity Standards 17 GFS Customer Service Representatives will be glad to help Application Notes 18-20 you open an account. For terms and conditions see Inside Analytical & Lab Chemicals 21 Back Cover. For complete Terms and Conditions, please visit: Troubleshooting Guide 22 termsandconditions.html Ordering2 3
  3. 3. ContinuousGFS Chemicals has been manufacturing standards forover twenty years for unique customer applications. The In-Spec® product line is constantly growing and currently includes standards to check not just photometric accuracy but also wave- length, stray light, and resolution for a variety of instrumentation.Many of our standards are specific to your industry. Some recent areas of focus have been short pathlength instruments and microplate readers used inSeTTINg The STaNDarD for many pharmaceutical and biotechSpeCTrophoToMeTrIC laboratories.aNalySIS & verIfICaTIoNContinuous improvement at GFSChemicals means:• Development of new standards for the latest instruments. • R&D for new uses and ® applications for the In-Spec® product line.• Investment in a new labora- Features Benefits tory to meet higher quality control Non-Toxic No disposal fees standards to meet the demands Green Alternative of the most stringent industries. Inexpensive Save cost compared to• Certification for ISO 17025 is Sealed cuvette alternatives underway. Easy to Use No wasted time with dilutions No special precautions• Experienced technical support to troubleshoot and answer your NIST Traceable Confidence in instrumentation & readings Meets regulatory requirements specific questions. Live Technical Assistance Assistance for method development Help with troubleshooting Customizable Made for your application Multiple pathlengths and wavelengths available Extensive data provided Manufactured by GFS In stock and ready to ship Trusted name for quality in the industry4 Improvement 3 5
  4. 4. Selection Gui de In-Spec® How to Use: Choose your wavelength of interest then go across the chart Photometric to find your closest target absorbance value. The top of the Standards column will identify the item number and product name Selection Guide associated with that value. UV Standards Wave- Uv-1 Uv-2 Uv-3 Uv-4 Uv-5 Uv-6 Uv-7 Uv-8 Uv-20 Uv-30 length #8301 #8302 #8303 #8304 #8305 #8306 #8307 #8308 #8309 #8310 250 0.086 0.127 0.177 0.305 0.347 0.447 0.513 0.570 1.135 1.664 254 0.081 0.120 0.165 0.283 0.323 0.416 0.477 0.528 1.054 1.551 In-Spec® UV In-Spec® Visible 260 0.075 0.112 0.152 0.259 0.297 0.382 0.437 0.484 0.967 1.420 Standards Standards 280 0.056 0.870 0.112 0.188 0.220 0.283 0.322 0.355 0.715 1.058 In-Spec® UV Standards are de- The In-Spec® Visible Standards 300 0.039 0.060 0.79 0.131 0.155 0.201 0.230 0.253 0.508 0.754 signed to check the photometric are designed to check the pho- 350 0.011 0.030 0.042 0.072 0.086 0.110 0.127 0.139 0.284 0.420 accuracy of a traditional spectro- tometric accuracy of a traditional photometer from 250nm up to spectrophotometer from 350nm 400 0.007 0.018 0.026 0.045 0.054 0.069 0.080 0.088 0.179 0.266 400nm. up to 850nm. Visible Standards UV Standards - Item Numbers Visible Standards-Item Numbers UV-1: 8301 Vis 0.05: 8311 Wave- vIS-0.5 vIS-0.1 vIS-0.3 vIS-0.5 vIS-0.8 vIS-1 vIS-4 UV-2: 8302 Vis 0.1: 8312 length #8311 #8312 #8322 #8313 #8314 #8315 #8316 UV-3: 8303 Vis 0.3: 8322 400 0.122 0.260 0.724 1.254 1.882 2.436 3.43 UV-4: 8304 Vis 0.5: 8313 450 0.082 0.177 0.500 0.871 1.323 1.780 3.326 UV-5: 8305 Vis 0.8: 8314 500 0.059 0.126 0.360 0.628 0.957 1.298 3.238 UV-6: 8306 Vis 1.0: 8315 550 0.043 0.093 0.270 0.470 0.719 0.975 3.12 UV-7: 8307 Vis 4.0: 8316 600 0.034 0.072 0.212 0.368 0.566 0.767 2.91 UV-8: 8308 UV-20: 8309 650 0.027 0.056 0.169 0.294 0.450 0.611 2.524 UV-30: 8310 700 0.022 0.045 0.137 0.239 0.365 0.497 2.111 Available as sealed quartz cuvettes or bottles (60mL, 125mL, 250mL, 750 0.018 0.037 0.111 0.195 0.299 0.406 1.740 500mL, 1L) 800 0.017 0.030 0.092 0.161 0.247 0.334 1.440 If you need a more precise value please see the customPlease note that the values in the table are representative values only and are to assist in picking out the appropriate standard for products section on page 16.your application. The actual value of the lot you receive may vary. The data provided is representative of a 10mm pathlength cell.6 7
  5. 5. ® ® In-Spec® In-Spec® Amaranth Patent Blue Standard Red Standard Item # 8416 Item #8414 This popular product can be The In-Spec® Amaranth Red standard customized to match a variety of was designed to complement the In- wavelength and concentration levels. Spec® Patent Blue but can be used ex- This reference standard is designed clusively for a variety of other processes. for small volume instruments and When used together, the In-Spec® Patent microplate readers but is equally Blue and Amaranth Red standards cover adept on a traditional spectropho- a wider range of wavelengths that are tometer. Typical applications include associated with applications in pharma- analyses involving DNA, RNA, nu- ceutical and biotech laboratories. This cleic acids, proteins, and enzymes. reference standard is similar to the In- A commonly utilized wavelength for Spec® Patent Blue standard in that it can this reference standard is 260nm. be used on microplate readers, traditional spectrophotometers, and small path- length instruments. Popular wavelengths for this standard are 280nm and 320nm. Sizes Available Sizes Available 60ml 60m l 125ml 125ml_ 250ml 250ml 500ml 500ml 1l 1l Sealed Cuvette Sealed Cuvette8 Standards 9
  6. 6. ® ® In-Spec® BCA Purple Standard In-Spec® Nano-Kit Item # 8587   Item # 8476 The newest addition to the In-Spec® The In-Spec® Nano-Kit was awarded for the product line came about due to innovation in solving the issue of validating short demand to validate colorimeters and pathlength instruments without a toxic material. spectrophotometers used in BCA pro- Prior to the kit’s introduction the only option was tein assays. The wavelength of interest hazardous potassium dichromate solutions. The for this application is 562nm. This In-Spec® Nano-Kit consists of our popular Patent reference standard is equally customi- Blue and Amaranth Red reference standards with zable as its Patent Blue and Amaranth standard data at 260nm, 280nm and 320nm. Red counterparts. The data is certified with a 1mm pathlength cu- vette eliminating the conversion from the 10mm Sizes Available pathlength reading which is typical with many 60ml other standards. 125ml The reference standards are guaranteed for 1 50ml year from the date of shipment unlike many com- 500ml peting materials that are a one-time use product. 1l10 Standards Nano-Kit 11
  7. 7. Wavelength Accuracy Wavelength Accuracy Standards The accuracy of your instrument’s wavelength scale is crucial when obtaining absorbance readings on your sample. A slight change of less than 1nm can cause an instrument’s read- ings to shift leading to incorrect data. This puts the laboratory at risk of passing a product that should have failed quality control. GFS Chemicals offers 3 different wave- length reference filters that are used to validate the wavelength scale. These materials will add confidence to your quality control process. Listed below are the wavelength refer- Didymium Oxide Wavelength Calibration Standard ence filters available with associated Item # 8378 298, 329, 355, 445, 469, 482, 512, 522, 576, 732, 740, 795, 802, 866nm peaks. These materials are available in sealed quartz cuvettes certified for 4 years or in HDPE bottles with a 1 year shelf life. Recertification is available for the sealed quartz cuvettes. Holmium Oxide Wavelength Calibration Standard Samarium Oxide Wavelength Calibration Standard Item # 8340 241, 250, 278, 287, 334, 345, 361, 386, 416, 451, 468, 485, 537, 64 nm Item # 8379 236, 267, 280, 290, 305, 318, 332, 345, 401, 416, 442, 464, 479 nm12 13
  8. 8. Stray Light Filters Filters Stray radiant light is energy reaching the detector that is not originating from the Potassium Dichromate Potassium Dichromate is used to check the photometric ac- curacy of an instrument. GFS Chemicals Inc offers 5 different concentration levels. Using 3 or more of these values will allow you to check the linearity of your instrument. These standards instrument light source. This can come from a variety of sources and impacts come sealed in 10mm quartz cuvettes or in bottles and are de- the readings output by the instrument. Stray light can be a problem at any wave- signed for use in the UV spectrum. The sealed standards are length but is more prevalent in the UV range. certified for 1 year from shipment and recertification service is available. Custom concentrations are available. Please see the The use of a stray light filter can verify that an external light source is not effecting custom section on page 16. the results. There are five commonly used materials to check the UV range of an instrument. Each standard has a ‘cut-off’ wavelength and eliminates light trans- Catalog Numbers mission below that level. Listed below are the standards offered and their associ- 20mg/L Item #8350 ated ‘cut-off’ wavelength. These are available in sealed quartz cuvettes certified for 40mg/L Item #8368 2 years or sealed HDPE bottles with a 1 year shelf life. Recertification is available. 60mg/L ltem #8369 80mg/L Item #8348 100mg/L Item #8372 Background Solution Item # 8349 Potassium Iodide: 260nm Item #: 8380 Lithium Carbonate: 227nm Item #: 8382 Potassium Chloride: 200nm Item #: 8384 Sodium Iodide: 260nm Item #: 8381 Sodium Chloride: 205nm Item #: 838314 15
  9. 9. ®You may have a unique application Custom Standardsthat requires verification of certainabsorbance values or wavelengths AMCO Clear®that aren’t listed for our stock products. Turbidity StandardsOur experienced staff has the ability Amco Clear® is an EPA approved primary standard for cali-to work with you to develop a refer- bration of turbidity sensors. It is available in a wide range ofence standard that will validate your values. It has a guaranteed one year shelf life even if you’veinstrument for your application. opened the bottle. The convenient submicron polymer sus- pensions are non-toxic primary calibration standards approvedPlease call technical support for by the US EPA for drinking water analysis for turbidity.assistance. Amco Clear® is “The Clear Choice in Turbidity.”In order to facilitate custom standarddevelopment there are several keypieces of data that are required.With this information, technicalsupport will be able to quickly assessyour needs.• Wavelength(s) of interest• Pathlength• Instrumentation make and model• Desired or sample absorbance rangeOur problem-solving lab team isavailable Monday through Fridayfrom 7:30 am to 4:00 pm EST. AMCO Clear® Advantages: • Safe, non-toxic and disposable • Requires no mixing or inverting • Easy-to-Use/ No dilutions or preparations • Stable—does not settle out of suspension • Accurate to 1% lot-to-lot Custom • Guaranteed one year shelf life • Available in a wide range of values • NIST Traceable • Custom Standards Available16 17
  10. 10. Application Notes Specific Uv absorbance (SUva) for Total organic Carbon (ToC) analysis Standards for validation of Microplate Readers & Short Pathlength Instruments Specific UV Absorbance (SUVA) is an analysis of drinking water that With growing demand to for smaller sample analysis, microplate readers are becoming uses UV absorbance at 254nm to identify dissolved organic carbon more common place in the biotech/pharmaceutical industry. (DOC) levels. UV absorbance is easily determined using a spectropho- These instruments are typically used in performing various tests in relation to the analysis tometer. Previous studies established a relationship between SUVA of DNA/ RNA, protein and enzymes. Many of the options that are used to verify the and the levels of humic substances that are removed during enhanced accuracy of these instruments are either expensive or limited in the scope that they can coagulation and/or enhanced softening. Utilizing this relationship allows check. for a cheap and easy method to determine whether enhanced coagula- GFS Chemicals has developed standards to validate the accuracy of instru- tion or softening is required based off of the exemption allowed in Stage ments with equal to or less than 1mm pathlengths. The most popular application at this 1 D/DBPR and Stage 2 D/DBPR. Removing the extra disinfection from time is for users checking at 260 and 280nm. the process can save a water district money when it comes to TOC This type of validation is where the In-Spec® Color standards work perfectly. removal. The color standards are designed to work with the built in software programs that manip- ulate the data obtained in the plate and displays it as optical density. The color standards Under Stage 1 D/DBPR, Standards Method 5910B was the only EPA have many useful wavelengths, as exhibited on page 8 and 9. These can also be made in approved method for SUVA determination. Stage 2 D/DBPR approved a wide range of concentrations to better match the values you see in your daily testing. EPA Method 415.3 (Revision 1.1, 2005) for TOC, DOC and SUVA The color standards are available in a wide range of sizes and have a 1 year determination. This one method not only combines all three tests, but shelf life so you can keep them on the shelf and ready to test your instrument when the also provides increased quality control procedures to ensure testing ac- need arises. All you need to do is pipet an aliquot into the wells on your plate and run curacy, including calibration verification procedures for all instruments them on the reader. Once the data is obtained you then compare that to the values on utilized in the method. the certificate of analysis to verify that the instrument is performing as it should. GFS Chemicals Inc. also offers Holmium Oxide solution in individual bottles so According to the EPA method, the verification of the spectrophotom- that you may verify the wavelength scale on your scanning microplate reader. This pro- eter can be done with a commercially prepared check standard. This vides a much cheaper alternative to sealed plates with Holmium Oxide or a sealed quartz method specifically mentions the In-Spec® photometric standards, cuvette. manufactured by GFS Chemicals, Inc, which were utilized by the By utilizing both the color standards and the Holmium Oxide solution you will EPA to validate their spectrophotometer when developing 415.3. The be able to say with confidence that the data you obtained on your microplate reader is In-Spec® photometric standards are NIST traceable, non-toxic, have a accurate based off NIST-traceable standards. one-year shelf life and require no preparation to use. Data provided at 254nm for In-Spec UV Standards 1 through 30.18 19
  11. 11. amaranth red and its use in the Wine Industry Notes The In-Spec® Amaranth Red standard is ideal for use in the Analytical and Laboratory Chemicals GFS manufactures over 6,000 organic and inorganic analytical reagent chemicals and specialties in a lean wine industry for some common testing that is performed on manufacturing operation. We have most chemicals colorimeters or spectrophotometers. Wavelengths commonly needed for laboratory, bench, analytical, quality control, tested in wine samples for color intensity, hue, and total phe- materials characterization, scientific or research work. nolic concentration analysis are 520nm, 420nm, & 280 nm. Our ultra-high purity double-distilled Veritas® and ACS The color standards can be used to validate your instrument so that you know that it is performing as it should or it can be Superior™ brand acids and Watermark® brand reagents used as an inter-lab check so that you know your instrumen- are used throughout the world. Our AMCO Clear® tation in one lab is matching that in another lab. Over time an Water Analysis Division manufactures the highest water instruments light source may dim and the values you obtain calibration standards available for water treatment plants will start to drift. around the globe. Knowing when this is occurring allows you to perform the • Watermark® pyridine-free • Silver Compounds necessary maintenance before the issue impacts the quality Karl fischer reagents • Reagents for Water of the wine that you are producing. Multiple concentrations • Veritas® hplC Solvents & Wastewater labs are available so that you may check a broad range of • HPLC/GC/UV-Vis Solvents • Organic Halogen Reagent – absorbance values. • Reagent Grade Solvents OHR/Halogen Level Field • Spectroscopy Products TestKits • Primary Standard Salts • Polyethylene Glycol • Normality Solutions (small quantities) • Colors and Indicators • Conductivity Standards and ph Buffers • AA and ICP Standard Solutions • Anion Standard Solutions • Standard Solutions for Trace Metal analysis • ACS Reagents • ASTM Type I Deionized water20 21
  12. 12. TroubleShooting Guide If your reference standards are Notes not reading within the tolerance levels stated on the certificate of analysis utilize the following guide prior to calling for technical support. Having the answers to the GFS Chemicals - Innovative Chemical Technology for Innovative Applications Chemists and technicians can connect to a single company with manufacturing resources in all key areas of chemistry. Customers rely on us for our problem solving ability. Our proven technologies, technical expertise, engineering excellence and attention to detail allow us to work hand-in-hand with our customers every step of the way – from of the Society of Chemical Manufacturers & Affili- ates (SOCMA) and the Ohio Chemistry Technology Council, GFS is committed to conducting our business with integrity and foresight. In addition, GFS is committed to full implementation of the SOCMA® ChemStewards® environmental, health, safety and security management system. Why Choose GFS Chemicals? startup to commercialization. GFS technology and • 80+ years of chemical manufacturing and chem- following questions will help expedite the troubleshooting process. products have kept us steps ahead of the indus- istry experience supporting process and try’s ever- changing chemical needs. commercial applications 1) When was the last time the lamp was changed? • Knowledgeable chemists and operators who GFS Chemicals began when Professor G. Freder- have a complete understanding of the a. GFS replaces the lamps in our instrument every 6 months, but with normal ick Smith started producing perchloric acid in manufacturing process use, changing the lamps every 2 years should be sufficient. a small factory in Columbus, Ohio. Since then, • Scale-up flexibility from small sample sizes to GFS has grown to become a leading producer of drums to metric tons, to ensure consistent quality b. Many older spectrophotometers do not have diagnostic capabilities to tell the specialty chemicals with performance-proven tech- from R&D to pilot to final production user when lamp energy is depleted or how many hours they have been in use. nologies for laboratory, analytical, scientific, • International expertise shipping products to 85+ research and development, pilot scale production, countries means your products will arrive on time, and manufacturing applications. meeting all regulatory compliance regulations 2) Are your cuvettes/cells clean, scratch-free and suitable for your range of use? • Technical support and resources every step of Project Customization the way a. All data provided has been obtained in a 10mm FUV quartz cuvette unless • Dedicated, responsive customer support stated in the certificate of analysis. Our production environment gives us the flexibility personnel and points of contact to offer you innovative solutions for all of your • Competitive pricing and reliable production b. Do not allow SDVB suspensions to dry on or inside your cells as it is difficult to chemical needs. GFS research and development lead times remove. can support you with the chemicals that your • U.S.-based quality manufacturing to ensure c. Disposable plastic cuvettes are generally not designed for the UV range unless application requires. Using our proven technology material availability, consistency and delivery to customize your product, we create customer- • Experienced staff who understand the complexi- specified on the package. specific item numbers and SKUs to streamline ties and dependencies of process chemistry and safeguard your process. Our formalized and repeatable ISO manufacturing ensures that you get Associations and Affiliations 3) Was the supplied background solution* used to provide a baseline that the same exact material every time. We package GFS Chemicals has been actively involved in the reads 0.0? according to your requirements in small packages scientific community for generations. Member- through drums. ships include: a. GFS has found that the “baseline” function alone may not provide a 0.000 ab- If your application requires more stringent condi- • American Chemical Society sorbance when the background solution is scanned later. It may take a combina- tions, we have experience with Good Manufac- • Society of Chemical Manufacturers and turing Practice (GMP) processes. We are a key Associates (SOCMA) tion of “Auto zero” and “Baseline” over the range of interest. supplier of critical materials for numerous Fortune • Tier II ChemSteward® Certified for Environmental b. See Certificate of Analysis for appropriate lot number of background solution. 100, GMP manufacturers. We understand the Health and Safety needs of our GMP customers – including pharma- • American Water Works Association and Stan- ceutical manufacturers. dard Methods for the Examination of Water and 4) How recently has your instrument had a full calibration that included wavelength Wastewater scale certification and stray light detection? Unmatched Product Quality • Water Environment Federation • Ohio Water Environment Association a. GFS has this performed twice yearly by the manufacturer GFS is an ISO 9001-2008 certified company. Our • ASTM International - Committees D19, E13, b. GFS provides NIST traceable wavelength and stray light check standards as quality control team guarantees product consis- E15 and E27 tency from lot-to-lot. We guarantee our products • American Council of Independent Laboratories- well. to meet the quality standards that we publish, and Associate member ship a Certificate of Analysis and a Material Safety • Ohio Manufacturers Association Data Sheet (MSDS) with every order. • Chemical and Specialties Management Council 5) What spectral bandwidth are you using on your instrument? • Ohio Chemistry Technology Council a. All provided data is for a 1nm bandwidth unless otherwise specified. Environmental and Community Stewardship • U.S. Chamber of Commerce b. Higher bandwidths often provide lower resolution and affect the values ob- • Ohio Chamber of Commerce GFS was founded on a business philosophy of • National Federation of Independent Businesses tained. community stewardship and respect for our envi- • National Association of Manufacturers ronmental resources. As a long-standing member • Tech Columbus *Supplied with In-Spec UV and Visible standards22 23
  13. 13. Team GFS Are you sure your instrumentation is properly reading your samples? Do your standard operating procedures require a vali- dation standard? Over time small changes in your instrumenta- tion can affect the data you obtain. Our reference standards are a high quality, in-expensive way to add confidence to your measurements. GFS Chemicals Inc offers a full line of spectrophotometer validation standards. • Wavelength reference standards • Photometric reference standards • Stray Light reference standards Contact information: e-mail 800-838-5567 extension 121 for technical assistance, pricing and availability (M-F 7:30 am to 4:00 pm Eastern Standard Time) 614-824-3299 740-881-5989 FAXCHEMICALS ® Visit for technical information or Order online at