Specialty Chemical Manufacturing Expertise Since 1928GFS ChemicalsChromatographyProducts2006-2007 PRODUCT CATALOG
Products For Chromatography And Material Separation/ PurificationThe GFS tradition of personal • Specialty Silicas, • Florisil®, Ion Exchangeservice and attention to detail Unmodified, 40, 60, 90, and Resins (p. 134-135)has attracted the attention of 150 Angstrom, GFSelectTM • Drierite®, Activatedexperts in chromatography grade (page 130), Bio- Carbons, Diatomaceouswho have long seen a need for refinedTM qualified by Earths (page 136) CHROMATOGRAPHYthis type of commitment to RNase, DNase and Protease • Specialty Reagents forproblem solving in separation testing. Chromatography (page 137)sciences. Our alignment with • Aluminas (Brockmann I: • Bio-RefinedTM Productsthe entrepreneurial resources Acidic, Basic, and Neutral); (page 138)of Diazem Corp. (Midland, MI) W. R. Grace silicas (page • Royer® Anion Exchangehas already provided industry 131-132) Resins and Precious Metalwith practical solutions to • Silica Gel Desiccants; Catalysts (page 139)environmental and process Molecular Sieves (page 133)problems. We invite you totalk to us about using this Diazem MCACportfolio of novel technolo- Flow through a Stainlessgies to address your materials Removal of Pt steel Column of anrecovery or separations from a fluid stream aqueous solution of Pt (0.01M) gave capture ofissues (note example at right). 0.029g of Pt on 0.3g of media which is 9.9% Pt• Custom Modified Silicas for relative to the mass of the media. Tough Separations Problems (page 124) Measurement of the effluent showed less than Diazem/Dow tailored 200ppb Pt at the baseline technologies for the effluent.• HPLC Solvents (page 126)• Macherey-Nagel NUCLEOSIL®, NUCLEODUR®, unmodified spherical and irregular silica gels (page 127-130) Plus access to the 27 36 45 full Macherey-Nagel Line of 0 9 18 mL of 0.1M Pt TLC and Column Chromatography Products
Silicas for Advanced The Vision The GFS Connection Separations from Diazem Diazem and the Dow Chemical The GFS commitment to offer Technologies Co. have announced a joint several prominent lines of sili- All the chromatographic sili- technology and licensing cas, including Macherey-Nagel cas on the following pages are agreement that will provide and W. R. Grace products, capable of being modified by the bio-tech market with spe- gives separations scientists a technology recently patented cialty chromatographic broad base of qualified prod- by Diazem Corp. The resulting sorbents and engineering ucts, traceable by lot analysis products frequently allow processes. This includes a under ISO-certification, which chromatographic separations critical range of state-of-the- may ultimately be enhanced that are unattainable by stan- art separation capabilities by Diazem/Dow technology. dard silica media. This novel suitable for geometric and Your customized project can silica/silane surface chemistry optical isomeric molecules, be managed from research toCHROMATOGRAPHY exhibits a utility and, more peptides and proteins. This commercialization with com- importantly, a predictability in technology will impact the plete confidence in the consis- solving real-life problems most challenging issues of tency of quality of the sorbent faced in separation science. analytical and preparative of your choice. GFS technical chromatography, and address and product support both industry needs from drug dis- help provide a new gateway The Technology covery to commercialization. to problem solving in cutting- Diazem’s Dual ZoneTM Material edge separation sciences. technology uses pore-diffu- sion control silylation with Unique Diazem Products two (or more) silane bonding Despite the customized agents to give selective bond- nature of Diazem products, ing of one chemical composi- GFS offers a selection of C-18 tion on the outer zone of a Diazem modified silicas for porous material and a differ- your consideration. These ent chemical composition on include substrates with poly- the inner zone. Each zone meric/hydrophilic character then has a different chemical useful in pharma-biotech sep- reactivity, and the process arations. New Diazem metal also allows for better silanol chelating media are also avail- control. Sorbents tailored via able from GFS. Please check in this technology can separate the alphabetical section under diverse materials, especially “Diazem.” biological substrates, more completely and efficiently than standard separations media.
New Diazem Corporation These resins have also shown • Recovery of precious metalsChelation Technology the ability to remove radioac- • Removal of metals that Diazem Corporation’s new tive radium from a contami- could otherwise interfereChelation adsorbent contin- nated source. The Diazem with analytical proceduresues to show promise in sever- MCAC phase reduced the • Purification of product oral markets. radium levels from 15 pico product streams curies to less than 1 pico • Removal or recovery ofThe new materials, referred to curie. Mercury levels were organic and inorganic acidas “metal chelation and acid reduced to below ppb levels. contaminantscapture” (MCAC) phases, are • Separation of proteins,based on the immobilization The MCAC phases are an peptides, or nucleotidesof hyperbranched macromole- important extension of • Removal of anion contami-cules on various support Diazem’s unique, proprietary nants such as phosphates Dual ZoneTM Materials CHROMATOGRAPHYmedia. These phases offerthe advantage of effectively Technology. In Dual ZoneTM Diazem Corporation has takenremoving metal cation and Materials, silanes are chemi- classical silane, surface sci-anion contamination from cally bonded to porous silica ence and polymer chemistrieschemical processes, waste- support media by precisely and used them in unconven-water discharge, and drinking controlling coverage density, tional ways to address marketwater. In addition, the materi- silanol capture and phase needs. Feel free to contactals are very effective acid polarity. Dual Zone Materials GFS regarding possible appli-scavengers. Applications can be chemically tailored to cations of interest to you.include removal of Fe, Co, Ni, achieve a range of separationCu, Zn, Pt, Pd, Pb, and Hg. Pt gradients for a variety of com-removal is carried out at a plex separations.rate up to 20% relative to theweight of the media. Anions can be removed with similar efficiency by using Diazem Anion Capture Phase. The possible uses of these materials include: • Recovery of transition-metal complexes used in homoge- neous catalytic processes • Treatment of waste streams to remove low levels of toxic anions
HPLC & Specialty Solvents GFS has decades of experi- ence with chemical distilla- tions, both organic and inor- ganic. Our growing experience with the Farchan LaboratoriesCHROMATOGRAPHY line of acetylenic and olefinic research intermediates has given us even more insights into the purification of volatile, moisture and temper- Each of our specialty solvents capillary gas chromatography ature sensitive materials. The is carefully analyzed and certi- with electron capture and grades of solvent offered in fied to meet our published flame ionization detectors. this catalog cover a full range specifications. The quality of of applications and sensitivi- each solvent far exceeds Ultra Low Cut-off, Distilled in ties. These include products reagent specifications set by Glass Solvents — consistently that are distilled-in-glass or the American Chemical pure solvents for: quartz, and HPLC solvents Society. Quality is controlled • HPLC where the emphasis is on not only by UV Spectro- • Gas Chromatography High Performance. photometry, but also by • Pesticide Residue Analysis • Spectrophotometry Min Assay % (by GLC) Max Water Max UV Cutoff Max Residue ACETONE 99.7 0.50% 330nm 3ppm ACETONITRILE 99.7 0.02% 190nm 3ppm CHLOROFORM 99.7 0.02% 245nm 3ppm CYCLOHEXANE 98.5 0.01% 195nm 3ppm DICHLOROMETHANE 99.8 0.01% 233nm 3ppm ETHYL ACETATE 99.6 0.05% 256nm 3ppm ETHYL ETHER 99.5 0.08% 218nm 3ppm FORMIC ACID 88.0 N/A N/A 1ppm HEPTANE 99.5 0.01% 200nm 3ppm HEXANE ___ 0.01% 200nm 3ppm METHANOL 99.8 0.08% 205nm 3ppm PENTANE 98.5 0.01% 195nm 3ppm PETROLEUM ETHER ___ 0.02% ____ 3ppm ISOPROPYL ALCOHOL 99.5 0.10% 205nm 3ppm TETRAHYDROFURAN 99.7 0.03% 215nm 3ppm TOLUENE 99.7 0.03% 284nm 3ppm 2,2,4-TRIMETHYLPENTANE 99.7 0.02% 210nm 3ppm
New Math For Silica Gel and Chromatography ProductsMACHEREY-NAGEL...90+Years in Products forChromatography Markets.GFS CHEMICALS 70+ YearsServing Analytical and CHROMATOGRAPHYResearch MarketsIt Adds Up...Partners with160+ years of combined expe-rience, just a phone call away.GFS is your gateway to a hostof chromatography productsfrom Macherey-Nagel GmbH, aleader in the development ofmedia and other products forseparation sciences for over90 years. GFS stocks a wide Larger quantities of these their consistent and well-range of M-N unmodified materials are also available. defined character:media, including such popular Please let us know how wetrademarked brands as can help you. • High bed stability due toNUCLEOSIL®, NUCLEODUR®, spherical particle shapePolygosil®, and Polygoprep®. NUCLEOSIL® Products – • High efficiency due toWhen your needs extend to narrow particle size the Industry Leadermodified silicas, cellulose, and NUCLEOSIL® is a group of distributionTLC products derived from totally porous spherical sili- • High separation perform-the list shown below, GFS can cas, featuring very pure, uni- ance due to optimizedalso provide you with those form SiO2 structure distrib- bonding propertiesmaterials in a timely manner. • High chemical and uted within a small (10 micron mechanical stability and finer), narrow particlePlease refer to the following • High load capacity and size range. Their outstandingtables and the alphabetical recovery rates performance in analytical assection for the core GFS prod- • High reproducibility from well as preparative columnucts from Macherey-Nagel. lot to lot chromatography stems from
All NUCLEOSIL® narrow-pore NUCLEODUR® describes a As the name implies, the dura- packings are stable to 8,500 new group of spherical silicas bility of this collection of sili- psi; wide bore packings are having average particle size cas is one of its primary fea- stable to greater than 4,000 falling in ranges greater than tures. The new particle size psi. This means there are no 10 microns. It takes the place ranges allow for greater sensi- limitations to the HPLC appli- of the NUCLEOPREP® group, tivity and versatility in its cations that can benefit from having the advantage of a broad range of applications. the NUCLEOSIL® product greater selection of narrow While the NUCLEOSIL® line of group. particle sizes for the 100 finer spherical silicas is espe- micron pore size: cially recommended for adsorption chromatography, SPHERICAL SILICAS the NUCLEODUR® line is also GFS # M-N Code Description well suited to partition chro- (angstrom - micron) IRREGULAR SILICAS matography.CHROMATOGRAPHY 2829 712000 Nucleosil 50-3 GFS # M-N Code Description 2830 712010 Nucleosil 50-5 (angstrom - micron) 2831 712020 Nucleosil 50-7 2862 711010 Polygosil 60-5 GFS# NUCLEODUR® 2832 712030 Nucleosil 50-10 2863 711280 Polygosil 60-7 2853 713610 Nucleodur 100-10 2864 711020 Polygosil 60-10 2854 713620 Nucleodur 100-16 2833 712360 Nucleosil 100-3 2865 711510 Polygosil 100-5 2855 713600 Nucleodur 100-20 2834 712040 Nucleosil 100-5 2835 712050 Nucleosil 100-7 2866 711520 Polygosil 100-7 2856 713630 Nucleodur 100-30 2836 712060 Nucleosil 100-10 2867 711530 Polygosil 100-10 2857 713551 Nucleodur 100-50 2837 712390 Nucleosil 120-3 2868 711600 Polygosil 300-7 2838 712400 Nucleosil 120-5 2839 712410 Nucleosil 120-7 2869 711890 Polygosil 1000-7 2840 712420 Nucleosil 120-10 The new NUCLEODUR® C18 2870 711001 Polygoprep 60-12 Gravity silica support and col- 2841 712430 Nucleosil 300-5 2871 711240 Polygoprep 60-20 umn packing exhibits excel- 2842 712440 Nucleosil 300-7 2872 711250 Polygoprep 60-30 2843 712450 Nucleosil 300-10 2873 711260 Polygoprep 60-50 lent long-term stability and 2874 711270 Polygoprep 60-80 column performance at low 2844 712680 Nucleosil 500-5 2875 711037 Polygoprep 60-130 and high pH levels. It is espe- 2845 712690 Nucleosil 500-7 2846 712700 Nucleosil 500-10 2876 711002 Polygoprep 100-12 cially well suited for method 2877 711003 Polygoprep 100-20 development because of its 2847 712710 Nucleosil 1000-5 2878 711540 Polygoprep 100-30 robust character and repro- 2848 712730 Nucleosil 1000-10 2879 711550 Polygoprep 100-50 2880 711033 Polygoprep 100-80 ducibility in RP-HPLC. Call or 2849 712850 Nucleosil 4000-5 2881 711034 Polygoprep 100-130 e-mail for a detailed technical 2850 712860 Nucleosil 4000-7 data sheet; please also inquire 2851 712870 Nucleosil 4000-10 2882 711004 Polygoprep 300-12 2883 711610 Polygoprep 300-20 regarding the availability of 2884 711620 Polygoprep 300-30 additional grades of NUCLEO- 2885 711630 Polygoprep 300-50 DUR® having larger pore sizes. 2886 711035 Polygoprep 1000-12 2887 711036 Polygoprep 1000-20 2888 711005 Polygoprep 1000-30 2889 711006 Polygoprep 1000-50
STANDARD SILICAS plates and sheets manufac- (60 Angstrom) tured from exactly the same grades (per your choice) of for Low Pressure silica or cellulose. Their range Column Chromatography of ready-to-use layers pro- and Thin Layer vides an added measure of Chromatography versatility to your problem- solving arsenal of resources. TLC Layers Macherey-Nagel silicas can be used immediately as received One of the advantages of – no tedious coating or working with Macherey-Nagel impregnation processes are TLC grades of silica (60 needed. These TLC plates Angstrom, below), and cellu- have homogeneous, smooth, CHROMATOGRAPHY lose (below) is the assurance well-adhering layers that that you also have prompt ensure reproducible, quantita- access to high quality TLC tive evaluations. Standard silica plates feature GFS # M-N Code Description (CC) silica particles between 5 and 2825 815380 230-400 mesh 17 microns. The thickness of 2826 815390 130-270 mesh the silica layer is 0.25 mm for 2827 815320 70-270 mesh analytical plates, and 2828 815330 70-230 mesh typically 0.5mm or 1mm thick (TLC) for preparative TLC layers. 2819 816330 5-25 micron 2820 816310 5-25 micron (GB) Most forms are available with 2821 816340 5-25 micron (FI) or without fluorescent indica- 2822 816410 5-25 micron (GB, FI) tor; this includes polyester 2823 816410HP 2-25 micron sheets, aluminum sheets, and 2824 816430HP 2-25 micron (GB) glass plates. Additional infor- mation on these and other TLC products is availableCELLULOSE - unmodified powders for TLC from the Macherey-Nagel Chromatography Guide. AllGFS # M-N Code Description these products are available through GFS.2890 816250 MN grade 301 (native, fibrous)2891 816260 MN grade 301 UV254 (with indicator)2892 816270 MN 301 HR (acid washed, de-fatted)2893 815050 MN 100 (native, fibrous, purified)2894 815060 MN 2100 (high purity grade)2895 815070 MN 2100ff (high purity, de-fatted)2896 815290 Avicel® (microcrystalline)
TM HPTLC Layers C-18 Silica GFSelect Silicas purity, acid washed, irregular silicas having pore sizes of 40, Reversed Phase Plates for Chromatography 60, 90, and 150 Angstrom In contrast to methodologies • Silica activity is stabilized at involving normal hydrophilic For the chromatographer who 7% water; pH (slurry) of 6.5- silicas, many difficult separa- wants to try something a little 7.0 is standard tions can be achieved using different at a reasonable cost, • TLC grade silicas are avail- lipophilic silica layers and the GFSelect™ line of chro- able in 5-15 micron particle reversed phase techniques. matographic silicas provides size distribution, 500 m2/gm Given the continuing develop- several distinct features: specific surface area, 60 ment of HPLC methods, the Angstrom pore diameter demand for TLC plates that • Automated processing pro- • Complementary products can be used for orienting pre- vides tight 40-63 micron parti- are available, including TLC plates, modified and reverseCHROMATOGRAPHY separations will also increase. cle size range where low fines Such systems frequently use are preferred for higher reso- phase silicas, and spherical C-18 and C-8 silanized silicas lution, and reproducibility silicas in 70, 100, 300, and 800 especially suited for the sepa- is critical Angstrom pore sizes ration of very polar • Advanced production tech- (hydrophilic) substrates nologies yield high which previously could only GFS # Pore Particle Size Range be separated by ion exchange 2797 40A 15-35 micron chromatography. Macherey- 2798 40A 40-63 micron Nagel has chosen to address 2799 40A 60-200 micron this need only with C-18 2800 40A 200-500 micron silanized 60 Angstrom (2-10 2801 60A 5-15 micron micron) silicas that have been 2802 60A 15-35 micron modified with different 2803 60A 40-63 micron degrees of silanization. Please 2804 60A 60-120 micron let us know if we can help you 2805 60A 120-200 micron select or provide the product 2806 60A 60-200 micron 2807 60A 200-500 micron that you need for your reversed phase separation 2808 90A 15-35 micron problem. 2809 90A 40-63 micron 2810 90A 60-200 micron Macherey-Nagel offers a broad 2811 90A 200-500 micron range of chromatography 2812 150A 40-63 micron products designed to address 2813 150A 60-120 micron the separations issues you are 2814 150A 60-200 micron most likely to face in your work. 2815 150A 120-200 micron Full information and valuable technical data are available in 2816 60A 5-15 micron, TLC/Gypsum the Macherey-Nagel 2817 60A 5-15 micron, TLC//Indicator 2818 60A 5-15 micron, TLC/Gyp.+ Ind. Chromatography Products guide.
The main section of the GFScatalog provides specifica- Specialty Silicas Grade 12 GFS #2780 28-200 mesh; may be particu-tions and order information from W. R. Grace larly useful in hydrocarbonfor these products. and steroid separations. Grace specialty silicas reflectOther attractive features of decades of experience in sur- Grade 15 GFS #2783this product line include face chemistry and related 30-60 mesh; narrower particle• availability of bulk quanti- separations technology. Their size distribution providesties of most materials expertise in both adsorption enhancement of separations• most grades can be modi- and absorption chemistries in achieved by Grade 12.fied to provide standard, cus- major commercial marketstom or reversed phase silicas includes industrial and spe- Grade 22 GFS #2784• ongoing development of cialty catalyst formulations. 60-200 mesh; complementsadsorbents for specialty appli- The following products find CHROMATOGRAPHY Grades 12 and 15.cations such as ion chelation favor in a wide variety of applications, and can be sup- plied in bulk quantities in a Grade 59 GFS #2788 8 mesh, intermediate density timely manner. (25 lb/cu.ft.), low surface area (300 m2/gm); a wide-pore, non-decrepitating buffer gel capable of high liquid adsorp- tion in applications such as color body removal; 99.6% SiO2. Grade 62 GFS #2789 60-200 mesh, 150 Angstrom; intermediate density, suitable for some catalyst support applications. Grade 922 GFS #2793 -200 mesh; general chromatog- raphy use. Grade 923 GFS #82 100-200 mesh, high purity chromatographic silica suit- able for ASTM D-1319-70.
Alumina, Brockmann I activity. Acidic and neutral grades are pro- Chromatographic duced by the measured addi- Grades from Camag tion of HCl (gas) to basic alu- mina, which is the natural product of calcination of alu- Chromatographic grade alumi- minum hydroxide. na is a white, fine-grained powder characterized by par- ticle size, surface area, porosi- ty and Brockmann activity. Minimal moisture content allows a designation of Brockmann I activity for theCHROMATOGRAPHY most popular grades of alumi- na. Less active grades (Brockmann II-V) can be achieved by adding controlled amounts of mois- ture to the material. All alumi- na can absorb moisture from the air, so care must be taken in storage and handling in order to preserve the Grade Designation GFS # 2619 GFS # 2620 GFS # 2618 Camag Product Code 5016-A-I basic 507-C-I neutral 504-C-I acidic pH of a 5% aqueous suspension (stirred) 9.5 +/- 0.5 7.0 +/- 0.5 4.5 +/- 0.5 Specific surface (m2/gm) 150 +/- 10% 150 +/- 10% 150 +/- 10% Brockmann activity I I I Mean pore diam. (Angstrom) 60 60 60 Pore volume (mL/gm) ca. 0.25 ca. 0.25 ca. 0.25 Chloride (mval/gm) nil 0.03 0.14 Fe2O3 (%) 0.01 – 0.03 0.01 – 0.03 0.01 – 0.03 Na2O (%) 0.25 – 0.4 0.25 – 0.4 0.25 – 0.4 Bulk density (gm/L) ca. 920 ca. 920 ca. 920 Granulometry >200 micron 10-20%; 160-200 micron 10-20%; 100-160 micron 35-80%; 71-100 micron 6-15%; 40-71 micron 2-10%; <40 micron 2-5%
Silica Gel Desiccants, this area; please inquire if youAdsorbents, Molecular Sieves would need information on more specialized silicas orfrom W. R. Grace zeolites that may be under development.The broad range of conditionsunder which moisture adsorp- These products all have 0.43tion plays a critical step in cc/gm pore volume, loss oncommercial and laboratory ignition (1750°F) of ca. 6.5%,processes has led to the and bulk density of ca. 45development of several types lb/cu.ft. Typical equilibriumof specialized products in the adsorption at 10% relativesilica gel and molecular sieve humidity is around 7 wt.%.families. The products listed CHROMATOGRAPHYbelow address basic needs in Feature Grade 03 Grade 13 Grade 40 Grade 41 Grade 47 Grade 48 (GFS #) 2779 2782 2785 1019 2786 2787 mesh on 8 30-200 6-14 3-9 1-2 mm 2-5 mm indicator none blue ind. none none blue ind. blue ind. use or drying need for fine MIL-D-3716B MIL-D-3716 99% SiO2 99% SiO2 criteria of gases particle size type II gr. H type I gr. H 750 m2/g 750 m2/g (internal surface area) Molecular Sieves 50% of the total volume of the solid. Drying of common liq- Grace Davison molecular uids and gases are typically sieves are convenient bead performed using 3, 4, or 5 forms of specific aluminosili- Angstrom pore diameter zeo- cates also known as zeolites. lites, chosen by virtue of the Adjusting the Group I or molecular dimension of the Group II component of the chemical to be adsorbed. zeolite formulation allows the Moisture removal is frequent- synthesis of material charac- ly carried out using 4A terized by cavities having spe- (4 Angstrom) molecular cific pore diameters. Internal sieves. The following GFS channels comprise close to products are available: TYPE FORM/SIZE GFS# FORM/SIZE GFS# FORM/SIZE GFS# 3A bead/4-8 mesh 2709 bead/8-12 mesh 963 powder/3-5 micron 2710 4A bead/4-8 mesh 2711 bead/8-12 mesh 964 powder/<5 micron 2712 5A bead/4-8 mesh 2713 bead/8-12 mesh 965 powder/3-5 micron 2714 13X bead/4-8 mesh 2715 bead/8-12 mesh 1067 powder/3-5 micron 2716 Please inquire regarding additional forms and types of zeolite/molecular sieve materials.
Florisil® from U.S. Silica DOWEX® - Fine Mesh The Florisil®line has found Spherical Ion Exchange Resins extensive use in preparative from Dow Chemical and analytical chromatogra- phy: Dowex® resins have a long and successful history in • Activated magnesium sili- areas such as fine chemical cate (“magnesium-silica and pharmaceutical column gel”), ca. 15.5% MgO, 84% separations. Based on a SiO2 microporous copolymer of • White, hard powdered mate- styrene and divinylbenzene rial which is a highly selec- (DVB), these beaded materi-CHROMATOGRAPHY tive adsorbent als are designed for maximum • TLC Grade, ultrafine (-325 resistance to oxidation, reduc- mesh) powder suitable for tion, mechanical wear and thin layer chromatography fracturing. Selectivity, consis- • Reagent Grade, activated at tency and reliability in column 675o C, suitable for pesticide separations makes Dowex® residue analysis resins the media of choice for • Standard Grade, activated at a broad range of applications. 650oC, general chromato- graphic adsorbent use The Dow resin nomenclature reflects the type of resin and Standard mesh cuts include degree of cross-linking to DVB 16-30, 30-60, 60-100, 60-200, that is present: 100-200, and -200. • Type I is a strong base (Cl- form) anion exchange resin; for example, a 1X2-100 resin contains 2% DVB, 50-100 mesh size. • 50W designates a strong acid (H+ form) cation exchange resin; Dowex 50WX4-200 has 4% DVB cross-linking and a particle size range of 100-200 mesh. If you don’t find the DOWEX® resin you need in the alpha- betical section of this catalog, please inquire.
GFS # Description Features2642 Amberlite IR-120(Plus) ® hydrogen form, strongly acidic, gel-type, 8% cross-linked; catalyst2643 Amberlite® IR-120, Na sodium form, gel-type, sulfonic acid functionality, some catalyst use2644 Amberlite® IRA-67 tertiary amine functionality, weakly basic, 1.6 meq/ml exch. capacity2645 Amberlite® IRA-400(Cl) quat-ammonium functionality, strongly basic, gel-type, chloride form2646 Amberlite® IRA-410 strongly basic gel-type resin also useful in catalytic applications2647 Amberlite® IRA-900 strongly basic, macroreticular resin, moderately high porosity2648 Amberlite® IRC-50 weakly acidic resin, selectively adsorbs alkaloids, amino acids CHROMATOGRAPHY2649 Amberlite® XAD-4 high surface area (750 m.sq./gm) polystyrene adsorbent, 20-60 mesh2652 Amberlyst® 15 strongly acidic, macroreticular, suitable for non-aqueous catalysis2653 Amberlyst® 15 (wet) strongly acidic, macroreticular, suitable for aqueous catalysis2654 Amberlyst® A-21 weakly basic (free base), macroreticular, non-aqueous use, AMBERLITE®/AMBERLYST® 20-60 mesh Resins and Polymeric Adsorbents from Rohm and Haas Polymeric materials technolo- gy at Rohm and Haas fostered the development of a variety of adsorbent media that are of particular interest to synthet- ic organic chemists and tech- nicians dealing with separa- tions and purifications in both aqueous and non-aqueous systems. Key characteristics are provided above. Please advise if you would like to see additional Rohm and Haas products offered in the GFS catalog. Our objective is to make GFS a more valuable resource for you.
Drierite® Calcium Sulfate non-channeling, chemically Diatomaceous Earth Desiccants from inert except toward water, Filter Aids constant in volume, neutral W. A. Hammond Co. Ltd. in pH. Products having trade names Drierite® is an all-purpose dry- such as Celite® and Celatom® For further information on are recognized for their deri- ing agent that is compatible desiccant efficiencies see the vation from naturally occur- with most chemical environ- series of articles by David R. ring silicate materials deposit- ments. It removes moisture Burfield et al., starting with J. ed over millennia via the efficiently from gases (includ- Org. Chem. 1977, 42, 3060-5. decomposition of aquatic ing air), refrigerants, organic liquids and solids. Tests have plants related to algae shown that the moisture Activated Carbon (diatoms). Their intricate remaining in gases dried with micro geometric forms, poros-CHROMATOGRAPHY Activated carbons are typical- ity and inertness give them Drierite® at ambient condi- ly amorphous powders or great versatility in chemical tions is 0.005 mg/liter. Air can granules that are character- absorption and filtration be dried to a dew point of ized by high adsorptivity for processes. GFS has used them -100°F, and organic liquids are many gases, vapors, colloidal for decades; we now offer often dried to the low part per solids, organic substrates and them in this catalog for your million range. Drying efficien- some inorganic species. convenience. cy is usually maintained even Common uses include decol- as application temperatures orization and purification or approach 100°C. Indicating separation of organic sub- forms containing 3% cobalt strates. GFS offers several chloride are available. See the popular grades; let us know if alphabetical section for GFS you can’t find the activated listings of both forms in 4, 6, carbon you need. 8, 10-20, and 20-40 mesh sizes. In liquid drying, Drierite® instantly absorbs 6.6 wt.% water by chemical action. In GFS # Code / Description drying gases, its water capaci- 2634 Darco 4-12 mesh granular, acid washed, lignite ty is 10 to 14 weight percent, carbon, general purpose use attributable to combined 2635 Darco 12-20 mesh granular, acid washed, lignite chemical and capillary action carbon, general purpose that is derived from a granule 2636 Darco 20-40 mesh granular, acid washed, lignite carbon, general purpose pore space volume of 38%. 717 Darco G-60, -100 mesh powder, high-purity, used Drierite® typically can be to purify/upgrade organics regenerated numerous times. 2634 Darco KB,-100 mesh wet powder (<30% H2O); It is non-toxic, non-corrosive, 1500 m2/g s.a. (dry), 1.5 cc/g pore volume (dry) 2640 Norit® RO 0.8, pellets, for liquid-phase adsorption
Specialty Reagents The following products find frequent use as reagents in ed by other standard proto- cols such as dinitrophenylhy- and Media for Ion Pair Chromatography: drazone derivatization. Chromatography GFS #1202 SILICIC ACIDS; WHITE Tetrabutylammonium hydrox- QUARTZ SILICA SAND ide, 1.0 M in water. The immense scope of chro- GFS #2262 matographic techniques andQUATERNARY AMMONIUM Tetrabutylammonium hydrox- tools is necessary to keepCOMPOUNDS for Ion-Pair ide, 0.4 M in water. pace with the growth andChromatography technical sophistication of GFS #2517 current separations technolo-The GFS line of Electrometric Tetrabutylammonium hydro- gies. Materials as elegant as CHROMATOGRAPHYand Analytical Reagent Grade gen sulfate, 98%, crystal. high purity silicic acid and as(EARG) quaternary ammoni- simple as silica sand are bothum compounds includes sev- GFS #2433 important resources for theeral products of particular use Tetrabutylammonium dihy- problem-solving chromatogra-in the field of Ion-Pair drogen phosphate, 98+%, pher. GFS now offers theseChromatography. Acquired crystal. materials for your conven-from Sachem, Inc. (Austin TX) ience:in 1999, these EARG quats are KETONE DERIVATIZINGhigh-purity materials especial- AGENTS GFS # 2794 Silicic acid, -100ly suitable for applications mesh, loss on ignition <16%,that must conform to analyti- An example of a superior for chromatographycal protocols having stringent ketone derivatizing agentpurity requirements. manufactured by GFS is: GFS # 2795 Silicic acid, <20Characterized by low levels of 2-diphenylacetyl-1,3-indane- micron, 99.9% for chromatog-alkali metals, chloride and dione-1-hydrazone raphyash, the EARG quats meet the (GFS #2366).criteria for use in various GFS # 2796 Silica sand, whitechromatographic protocols. This reagent facilitates the quartz, -50+70 mesh, suitable determination of volatile alde- for chromatography hydes and ketones by HPLC, even demonstrating great sen- sitivities at very low concen- trations. Using spectroscopic or fluorometric techniques, detection limits can be enhanced to 0.25 parts per bil- lion, surpassing limits provid-
GFS Products Better Buffers™ The GFS bio-analytical line toward metal ion complexa- tion. This is believed to be a for also includes a line of “Better primary source of interfer- Life Sciences Buffers”– Good’s buffer alter- natives that are better suited ences in protein analysis, where the complexation of to bioanalytical buffering situ- Cu(II) has been shown to be a ations that are sensitive to particular problem. The interferences caused by metal Better Buffers also allow the ion complexations, especially minimization of amine-metal GFS Bio-RefinedTM Products copper ion. These non-com- complexation promoted by We introduced our Bio- plexing tertiary amine buffers the presence of donor atoms Refined™ product line to meet cover the entire pH range (3- such as hydroxymethyl and the growing need in the Life 10) for which bioanalytical hydroxyethyl groups. Sciences and biochemical buffering is usually required.CHROMATOGRAPHY fields for high-purity, well- This includes most of the cur- This GFS line also includes characterized inorganic and rent focus on pH-dependent three buffers studied by Good organic salts for research and phenomena observed in bio- and co-workers: MES, MOPS, bio-analytical applications. logical and physiological and PIPES; the newly avail- These products are carefully studies. able MOBS (4-N-morpholino)- purified and checked to meet butanesulfonic acid, is also specific pH requirements as The N-substituents on the shown on the chart of pH well as for conformance to Better Buffers render the ranges of non-complexing optical absorbance and trace amine sterically inaccessible buffers. metal ion specifications. In response to the need for DESPEN greater assurances of bio- DEPP chemical purity in many prod- PIPBS ucts, GFS is developing proto- PIPPS cols for qualifying products for criteria such as the MOBS absence of DNase, RNase and MOPS protease. Detection method- PIPES ologies such as advanced fluo- TEEN rescence spectrophotometry will be used to ensure product MES conformance to these specifi- DESPEN cations. DEPP PIPBS PIPPS PIPES 2 4 6 8 10 12
Since the catalyst is not on lize molecules with several The Royer ® carbon, its contamination active sites, such asPalladium Catalyst- effects are significantly less nucleotides or proteins. A Breakthrough in than palladium on carbon. The Royer Palladium Catalyst A short time later, Royer andPalladium Catalysts is available in either powder colleagues2 demonstrated the form or beads. We also have feasibility of using formic acid manufactured and made avail- as a hydrogen donor for theBy employing a unique, able Platinum, Rhodium and removal of several protectingpatented Polyethylenimine- Ruthenium on the patented groups in the synthesis ofPolymer matrix to support Royer Substrate. peptides by catalytic transferpalladium metal, we have cre- hydrogenation on palladium.ated a new, highly effective The Royer® Products This idea was later improved upon when it was discovered CHROMATOGRAPHYpalladium catalyst which issuperior for hydrogenation While pursuing better meth- that the PEI “ghosts” wouldreactions. ods of peptide synthesis, complex palladium as well as Professor Gar Royer discov- other metals. Palladium wasThe Royer Palladium ered a method of preparing a complexed on the PEI thatCatalyst’s high surface area new polymeric material which was on silica beads and thenmakes it extremely easy to could be used for numerous reduced to the metal3. Thework with and eliminates the things. It was first reported in comparison of the rates ofneed to bother with the the literature in 19771 and a hydrogenolysis of Cbz-alaninemessy and hard-to-handle patent application was filed. using formic acid at roompalladium on carbon. The patent was granted in temperature and pressure are 1982, US 4,326,009. shown in Fig.1. This catalystThis Unique Palladium is available now as 1%Catalyst Offers These The first published applica- Palladium on PEI/SiO2 beadsAdvantages: tion of the material was as (GFS #1004) or powder (GFS catalyst in the hydrolysis of #1005) and 3% Palladium on1. Reusable p-nitrotrifluoroacetanilide and PEI/SiO2 powder (GFS #1068). Unlike other palladium cat- involved the preparation of a These palladium catalysts can alysts, the Royer Palladium polyethylenimine (PEI) also be used effectively with Catalyst can be reused up “ghost”. PEI was deposited on hydrogen gas for reduction to 10 times with little or no the surface of an alumina medium by decantation (in activity loss. bead, cross-linked with glu- contrast with the filtration2. Extremely low pyrophoric taraldehyde and the bead was required for palladium on car- potential then dissolved away, leaving bon) and have low pyrophoric3. Excellent stability the porous polymer shell or potential. That they are readi-4. Easy separation from “ghost”. The cross-linked ly reusable is shown in Fig. 2. reaction product polymer has many active sites5. Little or no contamination and will complex or immobi-
Further work with the affinity Figure 1. Rate of Pd-catalyzed of PEI polymers for anions led hydrogenolysis of Cbz-alanine to the application by Royer using formic acid as the hydro- and co-workers4 of several gen donor. Pd-PEI “ghosts”, 100 mg (•); Aldrich 10% Pd on char- compositions of PEI polymers coal, lot no. 031597, 10 mg to the separation of ( ); Research Inorganic/Organic nucleotide mixtures by col- Chemical 10% Pd on charcoal, umn chromatography. Several two different samples, 10 mg (X and O); Pd-black, 1 mg (∆). of these are shown in Figures 3, 4, and 5. The ion-exchange resins are available now as Royer® Anion Exchange Resins; PEI 40 and 100 are glutaraldehyde cross-linkedCHROMATOGRAPHY material on silica beads 20-40 mesh and silica gel 40-200 mesh, GFS #1000 and #1001. BZL-PEI 40 and 100 are benzy- lated derivatives of the glu- taraldehyde cross-linked Figure 2. Reuse of Royer product in the same mesh Palladium Catalyst. sizes, GFS #1002 and #1003, Atmospheric pressure hydrogenation of 0.1 mole respectively. PEI-X-40 and 100 nitrobenzene in 90 ml of are the Xama cross-linked methanol with 2 g of 1% materials, GFS #1065 and Royer Palladium Catalyst. #1066. 1. Meyers, W.E.; Royer, G.P.; J. Am. Chem. Soc., 99, 6141 (1977) 2. El Amin, B.; Anantharamaiah, G.M.; Royer, G.P.; Means, G.E.; J. Org. Chem., 44, 3442 (1979) 3. Coleman, D.R.; Royer, G.P.; J. Org. Chem., 45, 2268 (1980) 4. Watanabe, K; Chow W.-S.; Royer, G.P.;Anal. Biochem., 127, 155 (1982)
Figure 3. PEI-glutaraldehyde-sili-ca resolution of adenosine, AMP,ADP, and ATP. Conditions: columnsize 28 X 0.6 cm; flow rate, 1ml/min; one fraction equals 3 ml;adenosine (0.8 µmol) eluted withTris-HCl (pH 8, 10 mM), AMP (2.4µmol) with buffer containing 0.15M NaCl, ADP (2.3 µmol) and ATP(1.5 µmol) with a linear gradient(0.3-0.5 M). CHROMATOGRAPHYFigure 4. Separation of adeno-sine, AMP, ADP, and ATP onPEI-Xama silica gel. The col-umn (6 X 0.5 cm) was equili-brated with Tris-HCl (10 mM,pH 8); the nucleotides andadenosine (1 mg each) wereapplied in 2 ml of the samebuffer. Recoveries were quan-titative: flow rate, 1 ml/min; onefraction equals 3 ml.Figure 5. (A) Resolution of amixture of 3’:5’-cyclic AMP (0.13µmol) and MAP, ADP, ATP (1.3µmol each). Conditions: PEI-Xama silica gel column (6 X 0.5cm); flow rate, 1 ml/min; fractionvolume equals 3 ml. Tris-HCl (pH8, 10mM) was used to elute3’:5”-cyclic AMP. The NaCl gra-dient followed immediately.(B) In this case, the Tris-HClwas run for 30 min. Otherwise,conditions are identical to thoseof A.
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