recombinant DNA techolongy

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    recombinant DNA techolongy - Presentation Transcript

    1. Genetic Engineering & GMF Sami 05/26/09
    2. Content 05/26/09
      • Introduction to Genetic Engineering
      • Process of GE
      • Applications of GE
      • Genetically Modified Crop
      • Benefits of GMC
      • Risks of GMC
      • Labelling of GMC
    3. Genetic Engineering 05/26/09
    4. 05/26/09
      • Transgenes are inserted by using vectors like :
        • Bacterial Plasmids
        • Biolistic
        • Viruses
    5. Restriction Enzymes & Plasmids 05/26/09
    6. Gene gun 05/26/09 In Biolistic usage, a microscopic gold beads coated with a transgene & shot into the cell with a pulse of helium. Inside, transgene comes off the bead and integrates into the cell's genome through transcription
    7. Medical Application
        • Production of pharmaceuticals:
          • Treatment of diseases (human insulin & antibiotics).
          • Disease prevention. (hepatitis B vaccine)
      • Clonal propagation: a source of tissue or organ for transplantation avoid all problems of immunoin-compatibility
      • Gene therapy: Artificially replace the disease-causing gene with a normal allele. By using a virus vector to the target tissues.
      05/26/09
    8. Industrial Applications
      • Use of GM MO to make stone-wash jeans & to produce detergents.
      • GM MO with enhanced ability to break down environmental pollutants (E. coli to break down cellulose)
      • Fast growing Salmon
      05/26/09 Tomato cell Fish cell Fish DNA Denucleated fish egg
    9. Agricultural Application
      • Addition of Bt gene from Bacillus thuringiensis, as insecticide.
      • First whole GM food (Flavr Savr)
      05/26/09
    10. GMC
      • Selective breading
      • slow
      • imprecise
      • modification of genes occurs naturally
      • uncontrollable crops
      • Hidden traits
      • GE
      • very fast
      • precise
      • we introduce genes into an organism
      • increased food security for growth challenges
      • complete coding genome knowledge
      05/26/09
    11. GMC
      • Increase yields & more efficient use of lands
      • Increase the tolerance to adverse growing conditions
      • Reduced maturation time & Longer shelf life
      • Improve the nutrient composition
      • Improved resistance to disease, pests, weeds and herbicides
      • Improve sensory attributes
      • Improve processing characteristics so as to reduce wastage and environmental bills.
      • Eliminate allergenicity
      • Phytochemicals enhanced
      • Saves money and promotes higher profits
      05/26/09
    12. Effects of GMC
      • Production of toxin in pollen
      • Newly formed dangerous pathogens, super weeds & super pests
      • Triggering of catastrophic ecological imbalance
      • Inevitable out-crossing of transgenics’ with natures’
      • Unknown effect on men.
      • Domination of world food production by few companies & rich countries
      • Having enough but un fairly distributed
      05/26/09
    13. Pros and Cons of labeling systems 05/26/09
    14. Bibliography 05/26/09
      • The National Biotechnology Information Facility, New Mexico State University
      • The Science and Environmental Health Network, http:// www.sehn.org
      • http://www.Bio-Rad.com
      • http://www.ornl.gov/sci/techresources/Human_Genome/elsi/gmfood.shtml
      • http:// www.inspection.gc.ca/english/plaveg/pbo/pbobbve.shtml
      • http://www.bayercropscience.com/bayer/cropscience/cscms.nsf/id/OurCustomers
      • http:// allergies.about.com/library/blificbio.htm
      • Draper, D. (2002). Our Environment: A Canadian Perspective 2nd Ed . Scarborough: Thompson Canada Lmt.
      • Thank you

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    a basic introduction to Genetic engineering, its ap more

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