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Mmb1 lec2 qb2013
 

Mmb1 lec2 qb2013

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    Mmb1 lec2 qb2013 Mmb1 lec2 qb2013 Presentation Transcript

    • ACTIVITIES DATE ACTIVITY JULY 5 LAST PPT LECTURE JULY 9 VIRUTAL LABORATORY ACTIVITY JULY 12 FIRST EXAMINATION JULY 16 FREE DAY: LIBRARY WORK ON HOW TO DEVELOP YOUR GMO JULY 19-23 GROUP PRESENTATION (PPT) -What to Develop -Why Develop -How will you Develop -Dangers or Issues to Expect JULY 26 APPLICATION OF BIOTECHNOLOGY LECTURE SERIES
    • REQUIREMENTS •  GMO DEVELOPMENT PRESENTATION •  BIOWEEK EXHIBIT •  FIELD TRIP PORTFOLIO •  Expectations Before the Trip •  Learnings During the Trip •  Advocacy: Spreading knowledge of Biotechnology to Others
    • exams •  Examination 1: INTRODUCTION TO TECHNIQUES IN BIOTECHNOLOGY •  Examination 2: APPLICATIONS OF BIOTECHNOLOGY •  Examination 3: LAWS AND INTERNATIONAL GUIDELINES CONCERNING BIOTECHNOLOGY •  Take-home (online)
    • LAST MEETING: HOW WERE YOUR GENES ISOLATED? • Golden Rice • BT Corn • Edible Vaccine in Potato • GMO Soya • Recombinant Hepatitis B vaccine www.designingdigitally.com
    • GENE ISOLATION www.koihealth.org Source of Genes Primers will amplify target Gene of Interest Amplicon for “CONSTRUCT” www.invitrogen.com www.sciencedirect.com
    • POLYMERASE CHAIN REACTION www.neb.com www.teachersdomain.org
    • MAKING YOUR CONSTRUCT www.addgene.org
    • EXAMPLES OF RESTRICTION ENZYMES
    • TRANSFORMATION & SELECTION MARKERS bio.davidson.edu passel.unl.edu
    • BACTERIAL CLONING: THE PROCESS
    • CONFIRMATION OF EXPRESSION •  HOW WILL YOU KNOW IF THE GENE THAT YOU INSERTED IS EXPRESSED? viswagenbiotech.com en.wikipedia.org igece.org
    • www.designingdigitally.com
    • HOW ABOUT PLANTS? A plant part Is cultured Callus grows Shoots develop Shoots are rooted; plant grows to maturity clonal-solutions.com.au
    • DELIVERING GENES (Agrobacterium & The Gene Gun) apps.rhs.org.uk users.comcen.com.au
    • ehow.com weirdasianews.com cbc.ca
    • Cloning overview •  Organismal cloning produces one or more organisms genetically identical to the parent that donated the single cell •  may lead to production of stem cells for research and other applications •  CLONE: genetically identical individuals produced from one parent toonrefugee.com
    • Cloning plants bbc.co.uk •  A totipotent cell is one that can generate a complete new organism •  Plant cloning is used extensively in agriculture
    • Cloning animals •  Nuclear Transfer (e.g. BRANDY (2004) the cow and DOLLY (1997) the sheep) •  the nucleus of an unfertilized egg cell or zygote is replaced with the nucleus of a differentiated cell •  Embryo Splitting (production of high value herds and livestock breeds)
    • Nuclear transfer skinnerscience.com
    • Embryo splitting csus.edu
    • HUMAN CLONING & STEM CELLS Should we or Should we NOT?
    • 2 kinds of cloning ipscell.com
    • The ivf way to have a baby
    • Stem cell therapy stemcellsforhope.com
    • Stem cells for therapy home.honolulu.hawaii.edu
    • READING ASSIGNMENT: Success stories and fail stories using stem cell therapy Be sure to Identify the points of success and points of failure thinkprogress.org
    • NEXT MEETING: the virtual laboratory PER GROUP: 1. Laptop 2. Internet Source
    • ACTIVITY THE VIRTUAL LABORATORY Main reference: http://learn.genetics.utah.edu
    • WE WILL NEED AN I-NET CONNECTION •  http://learn.genetics.utah.edu/content/labs/ extraction/ •  http://learn.genetics.utah.edu/content/labs/pcr/ •  http://learn.genetics.utah.edu/content/labs/gel/ •  http://learn.genetics.utah.edu/content/labs/ microarray/ •  http://learn.genetics.utah.edu/content/tech/cloning/ clickandclone/
    • FOR DISCUSSION •  What is the significance of DNA isolation? •  When is a PCR useful? •  Why do bands appear in an electrophoresis? •  How can a microarray data reveal gene expression? •  When do you think cloning is not indicated?
    • NEXT MEETING: EXAM exam number 1