Mmb1 lec2 qb2013

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Mmb1 lec2 qb2013

  1. 1. ACTIVITIES DATE ACTIVITY JULY 5 LAST PPT LECTURE JULY 9 VIRUTAL LABORATORY ACTIVITY JULY 12 FIRST EXAMINATION JULY 16 FREE DAY: LIBRARY WORK ON HOW TO DEVELOP YOUR GMO JULY 19-23 GROUP PRESENTATION (PPT) -What to Develop -Why Develop -How will you Develop -Dangers or Issues to Expect JULY 26 APPLICATION OF BIOTECHNOLOGY LECTURE SERIES
  2. 2. REQUIREMENTS •  GMO DEVELOPMENT PRESENTATION •  BIOWEEK EXHIBIT •  FIELD TRIP PORTFOLIO •  Expectations Before the Trip •  Learnings During the Trip •  Advocacy: Spreading knowledge of Biotechnology to Others
  3. 3. exams •  Examination 1: INTRODUCTION TO TECHNIQUES IN BIOTECHNOLOGY •  Examination 2: APPLICATIONS OF BIOTECHNOLOGY •  Examination 3: LAWS AND INTERNATIONAL GUIDELINES CONCERNING BIOTECHNOLOGY •  Take-home (online)
  4. 4. LAST MEETING: HOW WERE YOUR GENES ISOLATED? • Golden Rice • BT Corn • Edible Vaccine in Potato • GMO Soya • Recombinant Hepatitis B vaccine www.designingdigitally.com
  5. 5. GENE ISOLATION www.koihealth.org Source of Genes Primers will amplify target Gene of Interest Amplicon for “CONSTRUCT” www.invitrogen.com www.sciencedirect.com
  6. 6. POLYMERASE CHAIN REACTION www.neb.com www.teachersdomain.org
  7. 7. MAKING YOUR CONSTRUCT www.addgene.org
  8. 8. EXAMPLES OF RESTRICTION ENZYMES
  9. 9. TRANSFORMATION & SELECTION MARKERS bio.davidson.edu passel.unl.edu
  10. 10. BACTERIAL CLONING: THE PROCESS
  11. 11. CONFIRMATION OF EXPRESSION •  HOW WILL YOU KNOW IF THE GENE THAT YOU INSERTED IS EXPRESSED? viswagenbiotech.com en.wikipedia.org igece.org
  12. 12. www.designingdigitally.com
  13. 13. HOW ABOUT PLANTS? A plant part Is cultured Callus grows Shoots develop Shoots are rooted; plant grows to maturity clonal-solutions.com.au
  14. 14. DELIVERING GENES (Agrobacterium & The Gene Gun) apps.rhs.org.uk users.comcen.com.au
  15. 15. ehow.com weirdasianews.com cbc.ca
  16. 16. Cloning overview •  Organismal cloning produces one or more organisms genetically identical to the parent that donated the single cell •  may lead to production of stem cells for research and other applications •  CLONE: genetically identical individuals produced from one parent toonrefugee.com
  17. 17. Cloning plants bbc.co.uk •  A totipotent cell is one that can generate a complete new organism •  Plant cloning is used extensively in agriculture
  18. 18. Cloning animals •  Nuclear Transfer (e.g. BRANDY (2004) the cow and DOLLY (1997) the sheep) •  the nucleus of an unfertilized egg cell or zygote is replaced with the nucleus of a differentiated cell •  Embryo Splitting (production of high value herds and livestock breeds)
  19. 19. Nuclear transfer skinnerscience.com
  20. 20. Embryo splitting csus.edu
  21. 21. HUMAN CLONING & STEM CELLS Should we or Should we NOT?
  22. 22. 2 kinds of cloning ipscell.com
  23. 23. The ivf way to have a baby
  24. 24. Stem cell therapy stemcellsforhope.com
  25. 25. Stem cells for therapy home.honolulu.hawaii.edu
  26. 26. READING ASSIGNMENT: Success stories and fail stories using stem cell therapy Be sure to Identify the points of success and points of failure thinkprogress.org
  27. 27. NEXT MEETING: the virtual laboratory PER GROUP: 1. Laptop 2. Internet Source
  28. 28. ACTIVITY THE VIRTUAL LABORATORY Main reference: http://learn.genetics.utah.edu
  29. 29. WE WILL NEED AN I-NET CONNECTION •  http://learn.genetics.utah.edu/content/labs/ extraction/ •  http://learn.genetics.utah.edu/content/labs/pcr/ •  http://learn.genetics.utah.edu/content/labs/gel/ •  http://learn.genetics.utah.edu/content/labs/ microarray/ •  http://learn.genetics.utah.edu/content/tech/cloning/ clickandclone/
  30. 30. FOR DISCUSSION •  What is the significance of DNA isolation? •  When is a PCR useful? •  Why do bands appear in an electrophoresis? •  How can a microarray data reveal gene expression? •  When do you think cloning is not indicated?
  31. 31. NEXT MEETING: EXAM exam number 1

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