Spectroscopy Worshop
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Spectroscopy Worshop

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Spectroscopy Worshop Spectroscopy Worshop Document Transcript

  • Spectroscopy: Calibration curv By Wilfredo Crespo The purpose of this seminar was to learn how to make a calibration curve to obtain astandard analysis of samples when using spectroscopy. Spectroscopy is the study of theinteraction between matter and radiated energy. In this seminar, we used the absorbancemethod to know the concentration of an analyte (sample). This method consists of passing asource of light through a sample and measuring how much light is absorbed by this sample. Theabsorbance value is proportional to the concentration of the analyte. In order to do thecalibration curve, we prepared a stock solution of potassium permanganate. (Then, a dilutesolution was made, and different concentrations were obtained from it in each of the 11 flasks weused. This sentence is not clear. Re-write it.) We again made dilute solutions from the lastdiluted solution and each of them was put in an absorbance spectroscopy machine for analysis.After obtaining the concentration numbers for each solution, we constructed a calibration curvein Excel. In conclusion, I refreshed my memory about some things I knew related to spectroscopy,and I also learned new things. In addition, I used some laboratory instruments that I had not everused before.