• Save
Genetic Modification in Maize, Papaya, Fritos® Corn Chips, and Natura® Soymilk
Upcoming SlideShare
Loading in...5
×
 

Genetic Modification in Maize, Papaya, Fritos® Corn Chips, and Natura® Soymilk

on

  • 787 views

 

Statistics

Views

Total Views
787
Views on SlideShare
787
Embed Views
0

Actions

Likes
0
Downloads
0
Comments
0

0 Embeds 0

No embeds

Accessibility

Categories

Upload Details

Uploaded via as Adobe PDF

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

Genetic Modification in Maize, Papaya, Fritos® Corn Chips, and Natura® Soymilk Genetic Modification in Maize, Papaya, Fritos® Corn Chips, and Natura® Soymilk Presentation Transcript

  • Frank SotoLester Rosario 5/6/2011
  •  Overview Genes to identify: 35S promoter, NOS terminator, and PSII Methodologya) DNA Extractionb) DNA Amplificationc) Electrophoresis of DNA Analysis of Results Conclusion
  •  GMO’s are organisms that have been modified through genetic engineering. In the United States, under guidelines issued by USDAs Animal and Plant Health Inspection Service, genetic engineering is defined as the genetic modification of organisms by recombinant DNA techniques (USDA, 2007) According to the USDA, crops have been genetically modified ever since 1996. These crops have all kind of modifications, such as: herbicide tolerance, pesticide tolerance, and bacterial resistance.
  • GenesStructure of a gene
  •  35S promoter from the cauliflower mosaic virus (CaMV 35S) Nopaline synthase (NOS) terminator from Agrobacterium tumefaciens Photosystem II (PSII)
  •  The genes 35S and NOS will appear in the gel. The 35S gene will be found at 203bp and NOS at 225bp. We believe the corn will be identified as GMO while the soymilk won’t. In addition, both the corn chips and the papaya will be indentified as GMO.
  •  DNA Extractiona) Weighing and Grinding of samples Non-GMO Maize Soymilkb) Pipette 50µL of Slurry into tube containing 50µL of 50µL of 50µL of Instagene matrix. sample sample sample slurry slurry slurryc) Flick tube and place 500µL of 500µL of 500µL of in a 95 degrees Instagene Instagene Instagene celsius water bath for matrix matrix matrix 5 min.d) Place tubes in microcentrifuge for 5 min.
  •  PCR PMM= Plant molecular marker GMM= Genetic moleculara) 20µL of indicated master mix are marker. dispensed to each Non-GMO PMM of the samples. Non-GMO GMMb) 20µL of DNA samples are dispensed to their GMO (+) PMM corresponding tubes. GMO (+) GMMc) Once the samples Mai. Test PMM have been dispensed, the Mai. Test GMM tubes are put in the thermal cycler. Soy Test PMM Soy Test GMM
  • 1 Molecular weight Ruler Electrophoresisa) The gel used was a 3% Agarose 2 Non-GMO PMM gel. 3 Non-GMO GMMb) To prepare for electrophoresis, 10µL of Orange Loading dye was deposited into each of the samples. 4 GMO (+) PMMc) 20µL of Molecular weight ruler(mwr) were deposited to wells 5 GMO (+) GMM to wells 1 and 10.d) 20µL of samples were dispensed 6 Mai. Test PMM to wells 2-9.e) Electrophoresis was run for 7 Mai. Test GMM 30min. at 100V.f) After electrophoresis, the gel was 8 Soy Test PMM stained with ethidium bromide pads for 20min. 9 Soy Test GMM 10 Molecular Weight Ruler
  • Frank’s Gel
  • Lester’s Gel
  •  Human errors may have lead to these results. The experiment should be repeated in order to obtain reliable results.