Texto adicional Texto adicional Texto adicional texto adicional Texto adicional texto adicional Lennis  Bedoya  Muñoz  ID:...
 
<ul><li>Anterior chamber  cornea, iris, pupil and lens. </li></ul><ul><li>Filled with a watery fluid called the aqueous hu...
<ul><li>Responsible for the  supply of nutrients. </li></ul><ul><li>Removal of metabolic wastes from the avascular tissues...
PRIMARY OPEN-ANGLE TYPE
 
CLOSED-ANGLE GLAUCOMA
<ul><li>Age  Especially  for  people  over  60  years  old </li></ul><ul><li>Family history </li></ul><ul><li>Race  Africa...
<ul><li>PREVIOUS STUDIES REPORTED SOME PROTEINS ARE ELVATATED IN PATIENTS WITH POAG </li></ul>
<ul><li>F irst coined in 1995  Complement of a cell line, tissue, or organism. </li></ul><ul><li>Today  Two definitions of...
<ul><li>PROTEOMIC USES </li></ul><ul><li>Identifying  components of the proteome. </li></ul><ul><li>Comparing the expressi...
 
 
PROTEOMICS  HAS BEEN USED TO STUDY EYE DISEASES
 
Understand Proteomic analysis of aqueous humor from patients with primary  open angle glaucoma  to identify the possible r...
 
Estudiar proteínas diferenciales  presentes en los pacientes con POAG.  Distribución de las proteínas diferenciales presen...
 
<ul><li>Recolección de las muestras  </li></ul><ul><li>BeijingTongren Eye Center </li></ul><ul><li>Capital médica universi...
 
<ul><li>Aprobado  por  la  declaración  de  Helsinki </li></ul><ul><li>Aprobación  del consentimiento por escrito </li></u...
<ul><ul><li>Comparar los resultados en los pacientes con POAG y en los pacientes control, analizando la presencia o ausenc...
 
Concentración total de proteínas presentes  en el humor acuoso
<ul><li>Método de Bradford </li></ul>
<ul><li>ELECTROFORESIS EN GEL DE 2 DIMENSIONES </li></ul><ul><li>Separación de proteínas  Movimiento en el campo eléctrico...
<ul><li>Aíslan proteínas obtenidas con la electroforesis  7 </li></ul><ul><li>Proteólisis con  tripsina modificada y  bica...
<ul><li>SDS-PAGE  Separación del AH </li></ul><ul><li>TBS  Lavado de la membrana </li></ul><ul><li>Determinar la presencia...
 
<ul><li>Pacientes con Glaucoma de ángulo abierto fue de 0.4382±0.1675 mg/ml (Rango=0.2796~0.6517 mg/ml). </li></ul><ul><li...
Patrones en el Gel de Electroforesis de 2 Dimensiones Paciente con Glaucoma Paciente Control
<ul><li>Pacientes con Glaucoma mostraron una mayor intensidad y número de manchas plateadas que los pacientes control. </l...
<ul><li>Se aislaron 7 proteínas encontradas en las manchas producidas en el gel de electroforesis, mediante el kit  nanoLC...
Identificación de Proteinas Prostaglandina Humana H2 D-Isomerasa Caspasa 14 transthyretin albumina transferrina albumina C...
<ul><li>La Anti-Prostaglandina Humana H2 D-isomerasa estaba presente en todas las muestras de Humor Acuoso. Indicando nive...
 
Doctors Argument Agreed Ferreira SM. “ Although POAG has been widely considered a multifactorial disease, IOP is the most ...
Author Argument Agreed Choi J “ Reducing IOP can still be considered the most beneficial treatment in terms of halting vis...
Doctors Argument Agreed Denecker G “ There were few reports about caspase 14 in eyes.Caspase-14 is a unique member of the ...
 
<ul><li>Molecular biology has allowed health personnel access to new diagnostic tools that every day as close to knowing t...
<ul><li>This study is very important because is a new opportunity to  to show the importance of proteomics in the diagnosi...
 
Juan Carlos Agudelo
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Glaucoma 1.1ppt

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Glaucoma 1.1ppt

  1. 1. Texto adicional Texto adicional Texto adicional texto adicional Texto adicional texto adicional Lennis Bedoya Muñoz ID: 000087035 Juan Carlos Agudelo Jiménez ID:000149094 MOLECULAR BIOLOGY UPB Proteomic analysis of aqueous humor from patients with primary open angle glaucoma. Xiaoming Duan .
  2. 3. <ul><li>Anterior chamber cornea, iris, pupil and lens. </li></ul><ul><li>Filled with a watery fluid called the aqueous humor. </li></ul><ul><li>Increased pressure of aqueous humor in the eyes </li></ul>Leading cause of irreversible visual impairment and blindness on a global level.
  3. 4. <ul><li>Responsible for the supply of nutrients. </li></ul><ul><li>Removal of metabolic wastes from the avascular tissues of the eye. </li></ul><ul><li>Contains proteins secreted from anterior segment tissues. </li></ul><ul><li>Protein levels in AH change in many eye diseases. </li></ul>
  4. 5. PRIMARY OPEN-ANGLE TYPE
  5. 7. CLOSED-ANGLE GLAUCOMA
  6. 8. <ul><li>Age Especially for people over 60 years old </li></ul><ul><li>Family history </li></ul><ul><li>Race African or Hispanic descent </li></ul><ul><li>Myopia </li></ul><ul><li>Raised intraocular pressure (IOP). </li></ul>No one knows exactly what causes glaucoma but some factors raise
  7. 9. <ul><li>PREVIOUS STUDIES REPORTED SOME PROTEINS ARE ELVATATED IN PATIENTS WITH POAG </li></ul>
  8. 10. <ul><li>F irst coined in 1995 Complement of a cell line, tissue, or organism. </li></ul><ul><li>Today Two definitions of proteomics are encountered. </li></ul>
  9. 11. <ul><li>PROTEOMIC USES </li></ul><ul><li>Identifying components of the proteome. </li></ul><ul><li>Comparing the expression of proteins between normal and diseased organs. </li></ul><ul><li>Determine how proteins interact with each other in vivo. </li></ul><ul><li>Identification and characterization of proteins post-translationally. </li></ul><ul><li>Study the structure and function of protein complexes. </li></ul><ul><li>Understand the organization of cells at the molecular level. </li></ul>
  10. 14. PROTEOMICS HAS BEEN USED TO STUDY EYE DISEASES
  11. 16. Understand Proteomic analysis of aqueous humor from patients with primary open angle glaucoma to identify the possible roles of these AH proteins in POAG and the respective relationships between POAG and elevated intraocular pressure (IOP).
  12. 18. Estudiar proteínas diferenciales presentes en los pacientes con POAG. Distribución de las proteínas diferenciales presentes en el humor acuoso. Comprender de mejor manera la relación entre la biología molecular y el desarrollo de los pacientes con POAG
  13. 20. <ul><li>Recolección de las muestras </li></ul><ul><li>BeijingTongren Eye Center </li></ul><ul><li>Capital médica universitaria de la China. </li></ul><ul><li>Antes de la cirugía </li></ul>
  14. 22. <ul><li>Aprobado por la declaración de Helsinki </li></ul><ul><li>Aprobación del consentimiento por escrito </li></ul><ul><li>El protocolo de estudio Aprobado por el comité de ética médica </li></ul><ul><li>Capital médica universitaria de Beijing </li></ul>
  15. 23. <ul><ul><li>Comparar los resultados en los pacientes con POAG y en los pacientes control, analizando la presencia o ausencia de factores que favorezcan el aumento de la PI </li></ul></ul>Paciente control Pacientes estudiados
  16. 25. Concentración total de proteínas presentes en el humor acuoso
  17. 26. <ul><li>Método de Bradford </li></ul>
  18. 27. <ul><li>ELECTROFORESIS EN GEL DE 2 DIMENSIONES </li></ul><ul><li>Separación de proteínas Movimiento en el campo eléctrico. </li></ul><ul><li>Determinar la cantidad de las proteínas presentes en los pacientes con POAG y en los control de acuerdo al grado de tinción obtenido. </li></ul><ul><li>Determinar si hay incremento en el volumen de las manchas de los pacientes con POAG y las pruebas control. </li></ul>
  19. 28. <ul><li>Aíslan proteínas obtenidas con la electroforesis 7 </li></ul><ul><li>Proteólisis con tripsina modificada y bicarbonato de amonio </li></ul><ul><li>Obtener sobrenadante y extraer más extractos de péptidos </li></ul><ul><li>Realizar cromatografía de líquidos y espectrofotometría de masas </li></ul><ul><ul><li>Identificación de proteínas </li></ul></ul>
  20. 29. <ul><li>SDS-PAGE Separación del AH </li></ul><ul><li>TBS Lavado de la membrana </li></ul><ul><li>Determinar la presencia de anticuerpos anti PGDS </li></ul><ul><ul><li>Confirmar la existencia de D- isomerasa de prostaglandina H2(PGDS) en los pacientes con glaucoma </li></ul></ul>
  21. 31. <ul><li>Pacientes con Glaucoma de ángulo abierto fue de 0.4382±0.1675 mg/ml (Rango=0.2796~0.6517 mg/ml). </li></ul><ul><li>Pacientes control fue de 3124±0.0567 mg/ml (rango=0.2484~0.3984 mg/ml). </li></ul>
  22. 32. Patrones en el Gel de Electroforesis de 2 Dimensiones Paciente con Glaucoma Paciente Control
  23. 33. <ul><li>Pacientes con Glaucoma mostraron una mayor intensidad y número de manchas plateadas que los pacientes control. </li></ul>Patrones en el Gel de Electroforesis de 2 Dimensiones Se encontró diferencias significativas entre el volumen % entre los pacientes con Glaucoma y los pacientes Control.
  24. 34. <ul><li>Se aislaron 7 proteínas encontradas en las manchas producidas en el gel de electroforesis, mediante el kit nanoLC MS/ MS para identificación. </li></ul>Identificación de Proteinas
  25. 35. Identificación de Proteinas Prostaglandina Humana H2 D-Isomerasa Caspasa 14 transthyretin albumina transferrina albumina Cistatina C AQGFTEDTIVFLPQTDK
  26. 36. <ul><li>La Anti-Prostaglandina Humana H2 D-isomerasa estaba presente en todas las muestras de Humor Acuoso. Indicando niveles altos en pacientes con Glaucoma de Ángulo Abierto. </li></ul>Analisis Western blott
  27. 38. Doctors Argument Agreed Ferreira SM. “ Although POAG has been widely considered a multifactorial disease, IOP is the most important known risk factor for the development of glaucomatous optic nerve damage.” Grus FH, Joachim SC, Sandmann S, Thiel U, Bruns K, Lackner KJ, Pfeiffer N. “ Most recent insights regarding POAG have come from studies on animal models. Other studies of AH typically focus only on proteins. Grus et al. has studied transthyretin changes in AH of POAG using surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) ProteinChip arrays and two-dimensional electrophoresis.”
  28. 39. Author Argument Agreed Choi J “ Reducing IOP can still be considered the most beneficial treatment in terms of halting visual field damage progression, even in normal pressure glaucoma. However, the mechanisms of elevated IOP have not been fully elucidated. At present, it is well known that elevated IOP could result from a variety of cell deaths in the trabecular meshwork. AH, the circulated intraocular fluid, includes many kinds of cytokines, which could likely play significant roles in the trabecular meshwork.”
  29. 40. Doctors Argument Agreed Denecker G “ There were few reports about caspase 14 in eyes.Caspase-14 is a unique member of the evolutionarily conserved family of cysteinyl aspartate-specific proteinases, which are mainly involved in inflammation and apoptosis. Previous studies demonstrated that caspase 8 and caspase 9 are the main kinds of caspase that play roles in the apoptosis of ocular tissues. According to this study, caspase14 might be involved in the apoptosis of ocular tissues by either directly mediating or inducing caspase 8 and caspase 9 activation.”
  30. 42. <ul><li>Molecular biology has allowed health personnel access to new diagnostic tools that every day as close to knowing the true etiology of diseases such as open-angle glaucoma and make decisions based on facts and effective and timely treatments. </li></ul><ul><li>A wide variety of proteins, still unknown, are present in many pathologies, that is why we must consider the molecular biology and engineering as fundamental molecular diagnostic methods. </li></ul>
  31. 43. <ul><li>This study is very important because is a new opportunity to to show the importance of proteomics in the diagnosis and treatment of deseases in which traditional methods has been limited. </li></ul><ul><li>this article made ​​a great contribution to molecular biology, medicine and scientific world Because researchers not satisfied with talk of glaucoma but also achieved to find the proteins that increased in POAG and analyze what is their role in this pathology. </li></ul><ul><li>  </li></ul>
  32. 45. Juan Carlos Agudelo

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