Eric C.Y., LEE   2011.Jan.04eric@nucleoti.de
Why this paper?
Sequencing Technology      Sampling     Amplification    Sequencing                     Bacterial       Separation1st   DNA...
Labeling
Nanopore by IBM
Oxford Nanopore Technology Enzyme
Tunneling Detector and      Nanopore
Si3N4•   High strength over a wide temperature range•   High fracture toughness•   High hardness•   Outstanding wear resis...
STM Picture
Why minutes?• The tunneling data are recorded at a rate  of 5kHz.       300,000,000/5,000=60,000 sec            = 1000 min...
Comparison         Reference:   (2010)
Conclusion• The successful depends on the chip  process.• In general temperature and environment,  it’s work.• More close ...
Thanks for your attention!
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Introduction to 3rd sequencing

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  • Introduction to 3rd sequencing

    1. 1. Eric C.Y., LEE 2011.Jan.04eric@nucleoti.de
    2. 2. Why this paper?
    3. 3. Sequencing Technology Sampling Amplification Sequencing Bacterial Separation1st DNA Sample Colonization or Synthetic2nd DNA Sample PCR Synthetic
    4. 4. Labeling
    5. 5. Nanopore by IBM
    6. 6. Oxford Nanopore Technology Enzyme
    7. 7. Tunneling Detector and Nanopore
    8. 8. Si3N4• High strength over a wide temperature range• High fracture toughness• High hardness• Outstanding wear resistance, both impingement and frictional modes• Good thermal shock resistance• Good chemical resistance
    9. 9. STM Picture
    10. 10. Why minutes?• The tunneling data are recorded at a rate of 5kHz. 300,000,000/5,000=60,000 sec = 1000 min ~ 16hr• In further studies, we intend to modify the electronics to perform tunneling experiments at the same acquisition rate as that of the ionic current (50 kHz). 100min ~1.6hr
    11. 11. Comparison Reference: (2010)
    12. 12. Conclusion• The successful depends on the chip process.• In general temperature and environment, it’s work.• More close to personalized medicine.
    13. 13. Thanks for your attention!

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