Ensura sep briefing (cell washing concentrate)

Stem Cell Partners A Regenerative Medicine CompanyStem Cell Partners LLCA Regenerative Medicine Company ENSURA-SEP POINT OF CARE CELL WASHING Contact: C t t Lee Bucklerlbuckler@celltherapygroup.com 778.278.6311 778 278 6311 1

IntroductionThe Ensura-Sep™ Canister is intended for thewashing of a cell suspension at the point of care.Cell washing and cell concentration are achievedin a single centrifugation step.Technology is density phase washing withproprietary centrifuge canister apparatus.Potential applications include removal of DMSO,Ficoll, enzymes, or red cell lysing reagents. y y g g 2

Summary of Cell Washing Method with Ensura-Sep: Slide 1 of 2 Add density blocking Ensura-Sep Tube Add cell suspension reagent to vertical Pellet cells by centrifugation fluid to reservoir chamber. chamber. (no intermingling of fluid phases occurs)Blocking fl id (bl e) has a densit that is less than cells (red circles) fluid (blue) density but greater than cell suspending fluid (yellow). 4

Summary of Cell Washing Method with Ensura-Sep: Slide 2 of 2 6. Resuspend cells & harvest5. Aspirate depleted fluid suspension cells by final aspiration into 5 from reservoir chamber. syringe.

Step 1: Add 2.4 mL of Density Blocking Reagent 6

Step 2: Add 10 - 40 mL of cell suspension 7

Step 3: Centrifuge (10 min for 2,000 x g) 8

Step 4: Empty reservoir chamber 9

Step 5: Harvest Cells (flushing to resuspend) 10

SummaryEnsura SepEnsura-Sep Cell Canisters enable thesimple and rapid washing/concentrationof a cell suspension to be achieved in asingle centrifugation step.We work with our clients to designcustom canisters and reagents to meettheir specific requirements. 11

Stem Cell Partners A Regenerative Medicine Company Stem Cell Partners LLC A Regenerative Medicine Company ENSURA-SEP POINT OF CARE CELL WASHING Contact: Lee Bucklerlbuckler@celltherapygroup.com 778.278.6311 1

http://www.celltherapygroup.com	32
http://celltherapygroup.com	8
http://app4.websitetonight.com	4

Ensura sep briefing (cell washing concentrate)

by Cell Therapy Group on Dec 13, 2011

Accessibility

Categories

Tags

Upload Details

Usage Rights

3 Embeds 44

Statistics

Ensura sep briefing (cell washing concentrate) — Presentation Transcript